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Protein

Adenylate kinase isoenzyme 5

Gene

AK5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP. Also displays broad nucleoside diphosphate kinase activity.2 Publications

Catalytic activityi

ATP + AMP = 2 ADP.
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei168 – 1681AMP 1By similarity
Binding sitei226 – 2261AMP 1By similarity
Binding sitei257 – 2571ATP 1By similarity
Binding sitei263 – 2631AMP 1By similarity
Binding sitei274 – 2741AMP 1By similarity
Binding sitei407 – 4071AMP 2By similarity
Binding sitei412 – 4121AMP 2By similarity
Binding sitei469 – 4691AMP 2By similarity
Binding sitei500 – 5001ATP 2By similarity
Binding sitei517 – 5171AMP 2By similarity
Binding sitei545 – 5451ATP 2; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi142 – 1476ATP 1By similarity
Nucleotide bindingi191 – 1933AMP 1By similarity
Nucleotide bindingi219 – 2224AMP 1By similarity
Nucleotide bindingi386 – 3916ATP 2By similarity
Nucleotide bindingi433 – 4353AMP 2By similarity
Nucleotide bindingi462 – 4654AMP 2By similarity

GO - Molecular functioni

  1. adenylate kinase activity Source: ProtInc
  2. ATP binding Source: UniProtKB-KW
  3. nucleoside diphosphate kinase activity Source: UniProtKB
  4. nucleoside kinase activity Source: Reactome

GO - Biological processi

  1. ADP biosynthetic process Source: ProtInc
  2. ATP metabolic process Source: InterPro
  3. dADP biosynthetic process Source: ProtInc
  4. nucleobase-containing small molecule interconversion Source: Reactome
  5. nucleobase-containing small molecule metabolic process Source: Reactome
  6. nucleoside diphosphate phosphorylation Source: UniProtKB
  7. nucleoside triphosphate biosynthetic process Source: UniProtKB
  8. pyrimidine ribonucleotide biosynthetic process Source: ProtInc
  9. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS07943-MONOMER.
BRENDAi2.7.4.3. 2681.
ReactomeiREACT_21330. Synthesis and interconversion of nucleotide di- and triphosphates.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase isoenzyme 5 (EC:2.7.4.3, EC:2.7.4.6)
Short name:
AK 5
Alternative name(s):
ATP-AMP transphosphorylase 5
Gene namesi
Name:AK5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:365. AK5.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. cytosol Source: Reactome
  3. microtubule organizing center Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24659.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 562562Adenylate kinase isoenzyme 5PRO_0000158930Add
BLAST

Proteomic databases

MaxQBiQ9Y6K8.
PaxDbiQ9Y6K8.
PRIDEiQ9Y6K8.

PTM databases

PhosphoSiteiQ9Y6K8.

Expressioni

Tissue specificityi

Brain specific.

Gene expression databases

BgeeiQ9Y6K8.
CleanExiHS_AK5.
ExpressionAtlasiQ9Y6K8. baseline and differential.
GenevestigatoriQ9Y6K8.

Organism-specific databases

HPAiHPA019128.
HPA057255.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi117670. 8 interactions.
IntActiQ9Y6K8. 8 interactions.
STRINGi9606.ENSP00000346577.

Structurei

Secondary structure

1
562
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi369 – 3757Combined sources
Beta strandi378 – 3836Combined sources
Helixi389 – 40012Combined sources
Beta strandi403 – 4064Combined sources
Helixi407 – 41711Combined sources
Helixi420 – 43011Combined sources
Helixi437 – 45115Combined sources
Beta strandi458 – 4614Combined sources
Helixi467 – 47610Combined sources
Beta strandi481 – 4877Combined sources
Helixi490 – 49910Combined sources
Helixi507 – 53529Combined sources
Beta strandi536 – 5427Combined sources
Helixi547 – 56115Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BWJX-ray2.30A/B/C/D/E/F366-562[»]
ProteinModelPortaliQ9Y6K8.
SMRiQ9Y6K8. Positions 133-317, 368-562.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y6K8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni133 – 316184Adenylate kinase 1Add
BLAST
Regioni162 – 19332NMPbind 1By similarityAdd
BLAST
Regioni256 – 26611LID 1By similarityAdd
BLAST
Regioni377 – 559183Adenylate kinase 2Add
BLAST
Regioni406 – 43530NMPbind 2By similarityAdd
BLAST
Regioni499 – 50911LID 2By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the adenylate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0563.
GeneTreeiENSGT00390000016215.
HOVERGENiHBG059001.
InParanoidiQ9Y6K8.
KOiK00939.
OMAiVFGEDTM.
OrthoDBiEOG7060S3.
PhylomeDBiQ9Y6K8.
TreeFamiTF313747.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00235. Adenylate_kinase_Adk. 2 domains.
InterProiIPR000850. Adenylat/UMP-CMP_kin.
IPR006267. AK1/5.
IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF47391. SSF47391. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR01360. aden_kin_iso1. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y6K8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNTNDAKEYL ARREIPQLFE SLLNGLMCSK PEDPVEYLES CLQKVKELGG
60 70 80 90 100
CDKVKWDTFV SQEKKTLPPL NGGQSRRSFL RNVMPENSNF PYRRYDRLPP
110 120 130 140 150
IHQFSIESDT DLSETAELIE EYEVFDPTRP RPKIILVIGG PGSGKGTQSL
160 170 180 190 200
KIAERYGFQY ISVGELLRKK IHSTSSNRKW SLIAKIITTG ELAPQETTIT
210 220 230 240 250
EIKQKLMQIP DEEGIVIDGF PRDVAQALSF EDQICTPDLV VFLACANQRL
260 270 280 290 300
KERLLKRAEQ QGRPDDNVKA TQRRLMNFKQ NAAPLVKYFQ EKGLIMTFDA
310 320 330 340 350
DRDEDEVFYD ISMAVDNKLF PNKEAAAGSS DLDPSMILDT GEIIDTGSDY
360 370 380 390 400
EDQGDDQLNV FGEDTMGGFM EDLRKCKIIF IIGGPGSGKG TQCEKLVEKY
410 420 430 440 450
GFTHLSTGEL LREELASESE RSKLIRDIME RGDLVPSGIV LELLKEAMVA
460 470 480 490 500
SLGDTRGFLI DGYPREVKQG EEFGRRIGDP QLVICMDCSA DTMTNRLLQR
510 520 530 540 550
SRSSLPVDDT TKTIAKRLEA YYRASIPVIA YYETKTQLHK INAEGTPEDV
560
FLQLCTAIDS IF
Length:562
Mass (Da):63,333
Last modified:August 31, 2009 - v2
Checksum:iCF7DB084924A782F
GO
Isoform 2 (identifier: Q9Y6K8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-365: Missing.

Note: It is unsure whether Met-1 or Met-5 is the initiator.

Show »
Length:197
Mass (Da):21,974
Checksum:iB63514CB7C3B58AF
GO
Isoform 3 (identifier: Q9Y6K8-3) [UniParc]FASTAAdd to basket

Also known as: Adenylate kinase 6

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.

Show »
Length:536
Mass (Da):60,315
Checksum:iD263FD0BABB4B82A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 101L → M in AAH36666 (PubMed:15489334).Curated
Sequence conflicti86 – 861E → G in AAH36666 (PubMed:15489334).Curated
Sequence conflicti182 – 1821L → P in AAO16520 (Ref. 2) Curated
Sequence conflicti182 – 1821L → P in AAH33896 (PubMed:15489334).Curated
Sequence conflicti321 – 3211P → S in AAO16520 (Ref. 2) Curated
Sequence conflicti343 – 3431I → T in AAO16520 (Ref. 2) Curated
Sequence conflicti348 – 3481S → Y in AAH33896 (PubMed:15489334).Curated
Sequence conflicti385 – 3851P → T in AAH33896 (PubMed:15489334).Curated
Sequence conflicti561 – 5611Missing in AAP97322 (Ref. 2) Curated
Sequence conflicti561 – 5611Missing in AAH33896 (PubMed:15489334).Curated
Sequence conflicti561 – 5611Missing in AAH12467 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti465 – 4651R → Q.
Corresponds to variant rs2803140 [ dbSNP | Ensembl ].
VAR_059435

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 365365Missing in isoform 2. 3 PublicationsVSP_037878Add
BLAST
Alternative sequencei1 – 2626Missing in isoform 3. 2 PublicationsVSP_037879Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF062595 mRNA. Translation: AAD27956.1.
AY171600 mRNA. Translation: AAO16520.2.
AF445193 mRNA. Translation: AAP97322.1.
CR541890 mRNA. Translation: CAG46688.1.
BC033896 mRNA. Translation: AAH33896.1.
BC036666 mRNA. Translation: AAH36666.1.
BC012467 mRNA. Translation: AAH12467.2.
CCDSiCCDS675.1. [Q9Y6K8-1]
CCDS676.1. [Q9Y6K8-3]
RefSeqiNP_036225.2. NM_012093.3. [Q9Y6K8-3]
NP_777283.1. NM_174858.2. [Q9Y6K8-1]
XP_006710635.1. XM_006710572.1. [Q9Y6K8-3]
UniGeneiHs.559718.
Hs.597002.

Genome annotation databases

EnsembliENST00000344720; ENSP00000341430; ENSG00000154027. [Q9Y6K8-3]
ENST00000354567; ENSP00000346577; ENSG00000154027. [Q9Y6K8-1]
GeneIDi26289.
KEGGihsa:26289.
UCSCiuc001dhn.3. human. [Q9Y6K8-1]

Polymorphism databases

DMDMi257051028.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF062595 mRNA. Translation: AAD27956.1.
AY171600 mRNA. Translation: AAO16520.2.
AF445193 mRNA. Translation: AAP97322.1.
CR541890 mRNA. Translation: CAG46688.1.
BC033896 mRNA. Translation: AAH33896.1.
BC036666 mRNA. Translation: AAH36666.1.
BC012467 mRNA. Translation: AAH12467.2.
CCDSiCCDS675.1. [Q9Y6K8-1]
CCDS676.1. [Q9Y6K8-3]
RefSeqiNP_036225.2. NM_012093.3. [Q9Y6K8-3]
NP_777283.1. NM_174858.2. [Q9Y6K8-1]
XP_006710635.1. XM_006710572.1. [Q9Y6K8-3]
UniGeneiHs.559718.
Hs.597002.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BWJX-ray2.30A/B/C/D/E/F366-562[»]
ProteinModelPortaliQ9Y6K8.
SMRiQ9Y6K8. Positions 133-317, 368-562.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117670. 8 interactions.
IntActiQ9Y6K8. 8 interactions.
STRINGi9606.ENSP00000346577.

PTM databases

PhosphoSiteiQ9Y6K8.

Polymorphism databases

DMDMi257051028.

Proteomic databases

MaxQBiQ9Y6K8.
PaxDbiQ9Y6K8.
PRIDEiQ9Y6K8.

Protocols and materials databases

DNASUi26289.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000344720; ENSP00000341430; ENSG00000154027. [Q9Y6K8-3]
ENST00000354567; ENSP00000346577; ENSG00000154027. [Q9Y6K8-1]
GeneIDi26289.
KEGGihsa:26289.
UCSCiuc001dhn.3. human. [Q9Y6K8-1]

Organism-specific databases

CTDi26289.
GeneCardsiGC01P077747.
H-InvDBHIX0020812.
HGNCiHGNC:365. AK5.
HPAiHPA019128.
HPA057255.
MIMi608009. gene.
neXtProtiNX_Q9Y6K8.
PharmGKBiPA24659.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0563.
GeneTreeiENSGT00390000016215.
HOVERGENiHBG059001.
InParanoidiQ9Y6K8.
KOiK00939.
OMAiVFGEDTM.
OrthoDBiEOG7060S3.
PhylomeDBiQ9Y6K8.
TreeFamiTF313747.

Enzyme and pathway databases

BioCyciMetaCyc:HS07943-MONOMER.
BRENDAi2.7.4.3. 2681.
ReactomeiREACT_21330. Synthesis and interconversion of nucleotide di- and triphosphates.

Miscellaneous databases

EvolutionaryTraceiQ9Y6K8.
GenomeRNAii26289.
NextBioi48625.
PROiQ9Y6K8.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Y6K8.
CleanExiHS_AK5.
ExpressionAtlasiQ9Y6K8. baseline and differential.
GenevestigatoriQ9Y6K8.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00235. Adenylate_kinase_Adk. 2 domains.
InterProiIPR000850. Adenylat/UMP-CMP_kin.
IPR006267. AK1/5.
IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF47391. SSF47391. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR01360. aden_kin_iso1. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel human adenylate kinase. cDNA cloning, expression analysis, chromosome localization and characterization of the recombinant protein."
    Van Rompay A.R., Johansson M., Karlsson A.
    Eur. J. Biochem. 261:509-517(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  2. "Cloning and characterization of a novel human gene, adenylate kinase 6."
    Li J., Ji C., Xie Y., Mao Y.
    Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
  3. Guo J.H., Yu L., Li D.
    Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Tissue: Brain and Liver.
  6. "Identification of two active functional domains of human adenylate kinase 5."
    Solaroli N., Panayiotou C., Johansson M., Karlsson A.
    FEBS Lett. 583:2872-2876(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
  7. "The human adenylate kinase 9 is a nucleoside mono- and diphosphate kinase."
    Amiri M., Conserva F., Panayiotou C., Karlsson A., Solaroli N.
    Int. J. Biochem. Cell Biol. 45:925-931(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.
  8. "Crystal structure of adenylate kinase 5."
    Structural genomics consortium (SGC)
    Submitted (JUN-2005) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 366-562 IN COMPLEX WITH AMP.

Entry informationi

Entry nameiKAD5_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6K8
Secondary accession number(s): Q5U622
, Q6FH66, Q7Z4T5, Q86YS0, Q8N464, Q96EC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2000
Last sequence update: August 31, 2009
Last modified: March 31, 2015
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.