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Protein

Adenylate kinase isoenzyme 5

Gene

AK5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP. Also displays broad nucleoside diphosphate kinase activity.2 Publications

Catalytic activityi

ATP + AMP = 2 ADP.1 Publication
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei168AMP 1By similarity1
Binding sitei226AMP 1By similarity1
Binding sitei257ATP 1By similarity1
Binding sitei263AMP 1By similarity1
Binding sitei274AMP 1By similarity1
Binding sitei407AMP 2By similarity1
Binding sitei412AMP 2By similarity1
Binding sitei469AMP 2By similarity1
Binding sitei500ATP 2By similarity1
Binding sitei517AMP 2By similarity1
Binding sitei545ATP 2; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi142 – 147ATP 1By similarity6
Nucleotide bindingi191 – 193AMP 1By similarity3
Nucleotide bindingi219 – 222AMP 1By similarity4
Nucleotide bindingi386 – 391ATP 2By similarity6
Nucleotide bindingi433 – 435AMP 2By similarity3
Nucleotide bindingi462 – 465AMP 2By similarity4

GO - Molecular functioni

  • adenylate kinase activity Source: ProtInc
  • ATP binding Source: UniProtKB-KW
  • nucleoside diphosphate kinase activity Source: UniProtKB
  • nucleoside kinase activity Source: Reactome

GO - Biological processi

  • ADP biosynthetic process Source: ProtInc
  • ATP metabolic process Source: InterPro
  • dADP biosynthetic process Source: ProtInc
  • nucleobase-containing small molecule interconversion Source: Reactome
  • nucleoside diphosphate phosphorylation Source: UniProtKB
  • nucleoside triphosphate biosynthetic process Source: UniProtKB
  • pyrimidine ribonucleotide biosynthetic process Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS07943-MONOMER.
ZFISH:HS07943-MONOMER.
BRENDAi2.7.4.3. 2681.
ReactomeiR-HSA-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase isoenzyme 5 (EC:2.7.4.3, EC:2.7.4.6)
Short name:
AK 5
Alternative name(s):
ATP-AMP transphosphorylase 5
Gene namesi
Name:AK5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:365. AK5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi26289.
OpenTargetsiENSG00000154027.
PharmGKBiPA24659.

Polymorphism and mutation databases

BioMutaiAK5.
DMDMi257051028.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001589301 – 562Adenylate kinase isoenzyme 5Add BLAST562

Proteomic databases

EPDiQ9Y6K8.
PaxDbiQ9Y6K8.
PeptideAtlasiQ9Y6K8.
PRIDEiQ9Y6K8.

PTM databases

iPTMnetiQ9Y6K8.
PhosphoSitePlusiQ9Y6K8.

Expressioni

Tissue specificityi

Brain specific.1 Publication

Gene expression databases

BgeeiENSG00000154027.
CleanExiHS_AK5.
ExpressionAtlasiQ9Y6K8. baseline and differential.
GenevisibleiQ9Y6K8. HS.

Organism-specific databases

HPAiHPA019128.
HPA057255.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi117670. 8 interactors.
IntActiQ9Y6K8. 8 interactors.
STRINGi9606.ENSP00000346577.

Structurei

Secondary structure

1562
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi369 – 375Combined sources7
Beta strandi378 – 383Combined sources6
Helixi389 – 400Combined sources12
Beta strandi403 – 406Combined sources4
Helixi407 – 417Combined sources11
Helixi420 – 430Combined sources11
Helixi437 – 451Combined sources15
Beta strandi458 – 461Combined sources4
Helixi467 – 476Combined sources10
Beta strandi481 – 487Combined sources7
Helixi490 – 499Combined sources10
Helixi507 – 535Combined sources29
Beta strandi536 – 542Combined sources7
Helixi547 – 561Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BWJX-ray2.30A/B/C/D/E/F366-562[»]
ProteinModelPortaliQ9Y6K8.
SMRiQ9Y6K8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y6K8.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni133 – 316Adenylate kinase 11 PublicationAdd BLAST184
Regioni162 – 193NMPbind 1By similarityAdd BLAST32
Regioni256 – 266LID 1By similarityAdd BLAST11
Regioni377 – 559Adenylate kinase 21 PublicationAdd BLAST183
Regioni406 – 435NMPbind 21 PublicationAdd BLAST30
Regioni499 – 509LID 21 PublicationAdd BLAST11

Sequence similaritiesi

Belongs to the adenylate kinase family.Curated

Phylogenomic databases

eggNOGiKOG3079. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00390000016215.
HOVERGENiHBG059001.
InParanoidiQ9Y6K8.
KOiK00939.
OMAiHINMTDL.
OrthoDBiEOG091G0OQ7.
PhylomeDBiQ9Y6K8.
TreeFamiTF313747.

Family and domain databases

CDDicd01428. ADK. 2 hits.
Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00235. Adenylate_kinase_Adk. 2 hits.
InterProiIPR000850. Adenylat/UMP-CMP_kin.
IPR033690. Adenylat_kinase_CS.
IPR006267. AK1/5.
IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF47391. SSF47391. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR01360. aden_kin_iso1. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y6K8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNTNDAKEYL ARREIPQLFE SLLNGLMCSK PEDPVEYLES CLQKVKELGG
60 70 80 90 100
CDKVKWDTFV SQEKKTLPPL NGGQSRRSFL RNVMPENSNF PYRRYDRLPP
110 120 130 140 150
IHQFSIESDT DLSETAELIE EYEVFDPTRP RPKIILVIGG PGSGKGTQSL
160 170 180 190 200
KIAERYGFQY ISVGELLRKK IHSTSSNRKW SLIAKIITTG ELAPQETTIT
210 220 230 240 250
EIKQKLMQIP DEEGIVIDGF PRDVAQALSF EDQICTPDLV VFLACANQRL
260 270 280 290 300
KERLLKRAEQ QGRPDDNVKA TQRRLMNFKQ NAAPLVKYFQ EKGLIMTFDA
310 320 330 340 350
DRDEDEVFYD ISMAVDNKLF PNKEAAAGSS DLDPSMILDT GEIIDTGSDY
360 370 380 390 400
EDQGDDQLNV FGEDTMGGFM EDLRKCKIIF IIGGPGSGKG TQCEKLVEKY
410 420 430 440 450
GFTHLSTGEL LREELASESE RSKLIRDIME RGDLVPSGIV LELLKEAMVA
460 470 480 490 500
SLGDTRGFLI DGYPREVKQG EEFGRRIGDP QLVICMDCSA DTMTNRLLQR
510 520 530 540 550
SRSSLPVDDT TKTIAKRLEA YYRASIPVIA YYETKTQLHK INAEGTPEDV
560
FLQLCTAIDS IF
Length:562
Mass (Da):63,333
Last modified:September 1, 2009 - v2
Checksum:iCF7DB084924A782F
GO
Isoform 2 (identifier: Q9Y6K8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-365: Missing.

Note: It is unsure whether Met-1 or Met-5 is the initiator.
Show »
Length:197
Mass (Da):21,974
Checksum:iB63514CB7C3B58AF
GO
Isoform 3 (identifier: Q9Y6K8-3) [UniParc]FASTAAdd to basket
Also known as: Adenylate kinase 6

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.

Show »
Length:536
Mass (Da):60,315
Checksum:iD263FD0BABB4B82A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10L → M in AAH36666 (PubMed:15489334).Curated1
Sequence conflicti86E → G in AAH36666 (PubMed:15489334).Curated1
Sequence conflicti182L → P in AAO16520 (Ref. 2) Curated1
Sequence conflicti182L → P in AAH33896 (PubMed:15489334).Curated1
Sequence conflicti321P → S in AAO16520 (Ref. 2) Curated1
Sequence conflicti343I → T in AAO16520 (Ref. 2) Curated1
Sequence conflicti348S → Y in AAH33896 (PubMed:15489334).Curated1
Sequence conflicti385P → T in AAH33896 (PubMed:15489334).Curated1
Sequence conflicti561Missing in AAP97322 (Ref. 2) Curated1
Sequence conflicti561Missing in AAH33896 (PubMed:15489334).Curated1
Sequence conflicti561Missing in AAH12467 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_059435465R → Q.Corresponds to variant rs2803140dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0378781 – 365Missing in isoform 2. 3 PublicationsAdd BLAST365
Alternative sequenceiVSP_0378791 – 26Missing in isoform 3. 2 PublicationsAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF062595 mRNA. Translation: AAD27956.1.
AY171600 mRNA. Translation: AAO16520.2.
AF445193 mRNA. Translation: AAP97322.1.
CR541890 mRNA. Translation: CAG46688.1.
BC033896 mRNA. Translation: AAH33896.1.
BC036666 mRNA. Translation: AAH36666.1.
BC012467 mRNA. Translation: AAH12467.2.
CCDSiCCDS675.1. [Q9Y6K8-1]
CCDS676.1. [Q9Y6K8-3]
RefSeqiNP_036225.2. NM_012093.3. [Q9Y6K8-3]
NP_777283.1. NM_174858.2. [Q9Y6K8-1]
XP_006710635.1. XM_006710572.3. [Q9Y6K8-3]
XP_016856497.1. XM_017001008.1. [Q9Y6K8-3]
XP_016856498.1. XM_017001009.1. [Q9Y6K8-3]
UniGeneiHs.559718.
Hs.597002.

Genome annotation databases

EnsembliENST00000344720; ENSP00000341430; ENSG00000154027. [Q9Y6K8-3]
ENST00000354567; ENSP00000346577; ENSG00000154027. [Q9Y6K8-1]
GeneIDi26289.
KEGGihsa:26289.
UCSCiuc001dhn.4. human. [Q9Y6K8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF062595 mRNA. Translation: AAD27956.1.
AY171600 mRNA. Translation: AAO16520.2.
AF445193 mRNA. Translation: AAP97322.1.
CR541890 mRNA. Translation: CAG46688.1.
BC033896 mRNA. Translation: AAH33896.1.
BC036666 mRNA. Translation: AAH36666.1.
BC012467 mRNA. Translation: AAH12467.2.
CCDSiCCDS675.1. [Q9Y6K8-1]
CCDS676.1. [Q9Y6K8-3]
RefSeqiNP_036225.2. NM_012093.3. [Q9Y6K8-3]
NP_777283.1. NM_174858.2. [Q9Y6K8-1]
XP_006710635.1. XM_006710572.3. [Q9Y6K8-3]
XP_016856497.1. XM_017001008.1. [Q9Y6K8-3]
XP_016856498.1. XM_017001009.1. [Q9Y6K8-3]
UniGeneiHs.559718.
Hs.597002.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BWJX-ray2.30A/B/C/D/E/F366-562[»]
ProteinModelPortaliQ9Y6K8.
SMRiQ9Y6K8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117670. 8 interactors.
IntActiQ9Y6K8. 8 interactors.
STRINGi9606.ENSP00000346577.

PTM databases

iPTMnetiQ9Y6K8.
PhosphoSitePlusiQ9Y6K8.

Polymorphism and mutation databases

BioMutaiAK5.
DMDMi257051028.

Proteomic databases

EPDiQ9Y6K8.
PaxDbiQ9Y6K8.
PeptideAtlasiQ9Y6K8.
PRIDEiQ9Y6K8.

Protocols and materials databases

DNASUi26289.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000344720; ENSP00000341430; ENSG00000154027. [Q9Y6K8-3]
ENST00000354567; ENSP00000346577; ENSG00000154027. [Q9Y6K8-1]
GeneIDi26289.
KEGGihsa:26289.
UCSCiuc001dhn.4. human. [Q9Y6K8-1]

Organism-specific databases

CTDi26289.
DisGeNETi26289.
GeneCardsiAK5.
H-InvDBHIX0020812.
HGNCiHGNC:365. AK5.
HPAiHPA019128.
HPA057255.
MIMi608009. gene.
neXtProtiNX_Q9Y6K8.
OpenTargetsiENSG00000154027.
PharmGKBiPA24659.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3079. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00390000016215.
HOVERGENiHBG059001.
InParanoidiQ9Y6K8.
KOiK00939.
OMAiHINMTDL.
OrthoDBiEOG091G0OQ7.
PhylomeDBiQ9Y6K8.
TreeFamiTF313747.

Enzyme and pathway databases

BioCyciMetaCyc:HS07943-MONOMER.
ZFISH:HS07943-MONOMER.
BRENDAi2.7.4.3. 2681.
ReactomeiR-HSA-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Miscellaneous databases

EvolutionaryTraceiQ9Y6K8.
GenomeRNAii26289.
PROiQ9Y6K8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000154027.
CleanExiHS_AK5.
ExpressionAtlasiQ9Y6K8. baseline and differential.
GenevisibleiQ9Y6K8. HS.

Family and domain databases

CDDicd01428. ADK. 2 hits.
Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00235. Adenylate_kinase_Adk. 2 hits.
InterProiIPR000850. Adenylat/UMP-CMP_kin.
IPR033690. Adenylat_kinase_CS.
IPR006267. AK1/5.
IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF47391. SSF47391. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR01360. aden_kin_iso1. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKAD5_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6K8
Secondary accession number(s): Q5U622
, Q6FH66, Q7Z4T5, Q86YS0, Q8N464, Q96EC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: September 1, 2009
Last modified: November 30, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.