Q9Y6K8 (KAD5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylate kinase isoenzyme 5
Short name=AK 5
EC=2.7.4.3

Alternative name(s):
ATP-AMP transphosphorylase 5

Gene names

Name:

AK5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP.
Catalytic activity	ATP + AMP = 2 ADP.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm.
Tissue specificity	Brain specific.
Sequence similarities	Belongs to the adenylate kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ADP biosynthetic process Traceable author statement Ref.1. Source: ProtInc ATP metabolic process Inferred from electronic annotation. Source: InterPro dADP biosynthetic process Traceable author statement Ref.1. Source: ProtInc nucleobase-containing small molecule interconversion Traceable author statement. Source: Reactome pyrimidine ribonucleotide biosynthetic process Traceable author statement Ref.1. Source: ProtInc signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	centrosome Inferred from direct assay. Source: HPA cytosol Traceable author statement. Source: Reactome
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate kinase activity Traceable author statement Ref.1. Source: ProtInc cAMP-dependent protein kinase regulator activity Inferred from electronic annotation. Source: InterPro nucleoside kinase activity Inferred from experiment. Source: Reactome
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y6K8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y6K8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-365: Missing.

Note: It is unsure whether Met-1 or Met-5 is the initiator.

Isoform 3 (identifier: Q9Y6K8-3)

Also known as: Adenylate kinase 6;

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 562

562

Adenylate kinase isoenzyme 5

PRO_0000158930

Regions

Nucleotide binding

382 – 390

9

ATP By similarity

Nucleotide binding

461 – 468

8

AMP By similarity

Sites

Binding site

406

1

AMP By similarity

Natural variations

Alternative sequence

1 – 365

365

Missing in isoform 2.

VSP_037878

Alternative sequence

1 – 26

26

Missing in isoform 3.

VSP_037879

Natural variant

465

1

R → Q.
Corresponds to variant rs2803140 [ dbSNP | Ensembl ].

VAR_059435

Experimental info

Sequence conflict

10

1

L → M in AAH36666. Ref.5

Sequence conflict

86

1

E → G in AAH36666. Ref.5

Sequence conflict

182

1

L → P in AAO16520. Ref.2

Sequence conflict

182

1

L → P in AAH33896. Ref.5

Sequence conflict

321

1

P → S in AAO16520. Ref.2

Sequence conflict

343

1

I → T in AAO16520. Ref.2

Sequence conflict

348

1

S → Y in AAH33896. Ref.5

Sequence conflict

385

1

P → T in AAH33896. Ref.5

Sequence conflict

561

1

Missing in AAP97322. Ref.2

Sequence conflict

561

1

Missing in AAH33896. Ref.5

Sequence conflict

561

1

Missing in AAH12467. Ref.5

Secondary structure

1

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562

Helix Strand Turn

Details...

Helix

369 – 375

7

Beta strand

378 – 383

6

Helix

389 – 400

12

Beta strand

403 – 406

4

Helix

407 – 417

11

Helix

420 – 430

11

Helix

437 – 451

15

Beta strand

458 – 461

4

Helix

467 – 476

10

Beta strand

481 – 487

7

Helix

490 – 499

10

Helix

507 – 535

29

Beta strand

536 – 542

7

Helix

547 – 561

15

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 1, 2009. Version 2. Checksum: CF7DB084924A782F	FASTA	562	63,333
	10 20 30 40 50 60 MNTNDAKEYL ARREIPQLFE SLLNGLMCSK PEDPVEYLES CLQKVKELGG CDKVKWDTFV 70 80 90 100 110 120 SQEKKTLPPL NGGQSRRSFL RNVMPENSNF PYRRYDRLPP IHQFSIESDT DLSETAELIE 130 140 150 160 170 180 EYEVFDPTRP RPKIILVIGG PGSGKGTQSL KIAERYGFQY ISVGELLRKK IHSTSSNRKW 190 200 210 220 230 240 SLIAKIITTG ELAPQETTIT EIKQKLMQIP DEEGIVIDGF PRDVAQALSF EDQICTPDLV 250 260 270 280 290 300 VFLACANQRL KERLLKRAEQ QGRPDDNVKA TQRRLMNFKQ NAAPLVKYFQ EKGLIMTFDA 310 320 330 340 350 360 DRDEDEVFYD ISMAVDNKLF PNKEAAAGSS DLDPSMILDT GEIIDTGSDY EDQGDDQLNV 370 380 390 400 410 420 FGEDTMGGFM EDLRKCKIIF IIGGPGSGKG TQCEKLVEKY GFTHLSTGEL LREELASESE 430 440 450 460 470 480 RSKLIRDIME RGDLVPSGIV LELLKEAMVA SLGDTRGFLI DGYPREVKQG EEFGRRIGDP 490 500 510 520 530 540 QLVICMDCSA DTMTNRLLQR SRSSLPVDDT TKTIAKRLEA YYRASIPVIA YYETKTQLHK 550 560 INAEGTPEDV FLQLCTAIDS IF
	Isoform 2 [UniParc]. Checksum: B63514CB7C3B58AF	FASTA	197	21,974
	10 20 30 40 50 60 MGGFMEDLRK CKIIFIIGGP GSGKGTQCEK LVEKYGFTHL STGELLREEL ASESERSKLI 70 80 90 100 110 120 RDIMERGDLV PSGIVLELLK EAMVASLGDT RGFLIDGYPR EVKQGEEFGR RIGDPQLVIC 130 140 150 160 170 180 MDCSADTMTN RLLQRSRSSL PVDDTTKTIA KRLEAYYRAS IPVIAYYETK TQLHKINAEG 190 TPEDVFLQLC TAIDSIF
	Isoform 3 (Adenylate kinase 6) [UniParc]. Checksum: D263FD0BABB4B82A	FASTA	536	60,315
	10 20 30 40 50 60 MCSKPEDPVE YLESCLQKVK ELGGCDKVKW DTFVSQEKKT LPPLNGGQSR RSFLRNVMPE 70 80 90 100 110 120 NSNFPYRRYD RLPPIHQFSI ESDTDLSETA ELIEEYEVFD PTRPRPKIIL VIGGPGSGKG 130 140 150 160 170 180 TQSLKIAERY GFQYISVGEL LRKKIHSTSS NRKWSLIAKI ITTGELAPQE TTITEIKQKL 190 200 210 220 230 240 MQIPDEEGIV IDGFPRDVAQ ALSFEDQICT PDLVVFLACA NQRLKERLLK RAEQQGRPDD 250 260 270 280 290 300 NVKATQRRLM NFKQNAAPLV KYFQEKGLIM TFDADRDEDE VFYDISMAVD NKLFPNKEAA 310 320 330 340 350 360 AGSSDLDPSM ILDTGEIIDT GSDYEDQGDD QLNVFGEDTM GGFMEDLRKC KIIFIIGGPG 370 380 390 400 410 420 SGKGTQCEKL VEKYGFTHLS TGELLREELA SESERSKLIR DIMERGDLVP SGIVLELLKE 430 440 450 460 470 480 AMVASLGDTR GFLIDGYPRE VKQGEEFGRR IGDPQLVICM DCSADTMTNR LLQRSRSSLP 490 500 510 520 530 VDDTTKTIAK RLEAYYRASI PVIAYYETKT QLHKINAEGT PEDVFLQLCT AIDSIF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel human adenylate kinase. cDNA cloning, expression analysis, chromosome localization and characterization of the recombinant protein." Van Rompay A.R., Johansson M., Karlsson A. Eur. J. Biochem. 261:509-517(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"Cloning and characterization of a novel human gene, adenylate kinase 6." Li J., Ji C., Xie Y., Mao Y. Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[3]	Guo J.H., Yu L., Li D. Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Tissue: Brain and Liver.
[6]	"Crystal structure of adenylate kinase 5." Structural genomics consortium (SGC) Submitted (JUL-2005) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 366-562 IN COMPLEX WITH AMP.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF062595 mRNA. Translation: AAD27956.1.
AY171600 mRNA. Translation: AAO16520.2.
AF445193 mRNA. Translation: AAP97322.1.
CR541890 mRNA. Translation: CAG46688.1.
BC033896 mRNA. Translation: AAH33896.1.
BC036666 mRNA. Translation: AAH36666.1.
BC012467 mRNA. Translation: AAH12467.2.

IPI

IPI00743623.
IPI00844054.
IPI00943762.

RefSeq

NP_036225.2. NM_012093.3.
NP_777283.1. NM_174858.2.

UniGene

Hs.559718.
Hs.597002.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BWJ	X-ray	2.30	A/B/C/D/E/F	366-562	[»]

ProteinModelPortal

Q9Y6K8.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9Y6K8. 8 interactions.

STRING

9606.ENSP00000346577.

PTM databases

PhosphoSite

Q9Y6K8.

Polymorphism databases

DMDM

257051028.

Proteomic databases

PaxDb

Q9Y6K8.

PRIDE

Q9Y6K8.

Protocols and materials databases

DNASU

26289.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000344720; ENSP00000341430; ENSG00000154027.
ENST00000354567; ENSP00000346577; ENSG00000154027.

GeneID

26289.

KEGG

hsa:26289.

UCSC

uc001dhn.3. human.

Organism-specific databases

CTD

26289.

GeneCards

GC01P077747.

H-InvDB

HIX0020812.

HGNC

HGNC:365. AK5.

HPA

HPA019128.

MIM

608009. gene.

neXtProt

NX_Q9Y6K8.

PharmGKB

PA24659.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0563.

HOVERGEN

HBG059001.

InParanoid

Q9Y6K8.

KO

K00939.

OMA

LQLCTAI.

OrthoDB

EOG4M0F1B.

PhylomeDB

Q9Y6K8.

Enzyme and pathway databases

BioCyc

MetaCyc:HS07943-MONOMER.

Reactome

REACT_111217. Metabolism.

Gene expression databases

ArrayExpress

Q9Y6K8.

Bgee

Q9Y6K8.

CleanEx

HS_AK5.

Genevestigator

Q9Y6K8.

GermOnline

ENSG00000154027. Homo sapiens.

Family and domain databases

InterPro

IPR000850. Adenylate_kin.
IPR006267. Adenylate_kin1.
IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
IPR007858. Dpy-30_motif.
IPR027417. P-loop_NTPase.
[Graphical view]

PANTHER

PTHR23359. PTHR23359. 1 hit.

Pfam

PF00406. ADK. 2 hits.
PF05186. Dpy-30. 1 hit.
[Graphical view]

PRINTS

PR00094. ADENYLTKNASE.

SUPFAM

SSF47391. cAMP-dep_prot_kin_reg_I/II_a/b. 1 hit.
SSF52540. SSF52540. 2 hits.

TIGRFAMs

TIGR01360. aden_kin_iso1. 1 hit.

PROSITE

PS00113. ADENYLATE_KINASE. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

ChEMBL

CHEMBL2928.

EvolutionaryTrace

Q9Y6K8.

GenomeRNAi

26289.

NextBio

48625.

SOURCE

Search...

Entry information

Entry name

KAD5_HUMAN

Accession

Primary (citable) accession number: Q9Y6K8
Secondary accession number(s): Q5U622 , Q6FH66, Q7Z4T5, Q86YS0, Q8N464, Q96EC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	September 1, 2009
Last modified:	May 29, 2013
This is version 119 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.