Q9Y6J9 (TAF6L_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L

Alternative name(s):
PCAF-associated factor 65-alpha
Short name=PAF65-alpha

Gene names

Name:	TAF6L
Synonyms:	PAF65A

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	622 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex.
Subunit structure	The PCAF complex is composed of a number of TBP-associated factors (TAFS), such as TAF5, TAF5L, TAF6, TAF6L, TAF9, TAF10 and TAF12, PCAF, and also PCAF-associated factors (PAFs), such as TADA2L/ADA2, TADA3L/ADA3 and SPT3. Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. Ref.1
Subcellular location	Nucleus Ref.1 Ref.5.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.7
Sequence similarities	Belongs to the TAF6 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	chromatin remodeling Traceable author statement. Source: ProtInc histone H3 acetylation Inferred from direct assay Ref.5. Source: UniProtKB regulation of sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro regulation of transcription from RNA polymerase II promoter Traceable author statement. Source: ProtInc transcription initiation, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	STAGA complex Inferred from direct assay Ref.5. Source: UniProtKB histone deacetylase complex Traceable author statement. Source: ProtInc
Molecular function	DNA binding Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction Ref.1. Source: IntAct transcription coactivator activity Inferred from direct assay Ref.5. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TAF9	Q16594	3	EBI-743984,EBI-712521

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 622	622	TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L		PRO_0000118879
Amino acid modifications
	Modified residue	85	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q9Y6J9 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: F294E71AF7D4C8C3	FASTA	622	67,814
	10 20 30 40 50 60 MSEREERRFV EIPRESVRLM AESTGLELSD EVAALLAEDV CYRLREATQN SSQFMKHTKR 70 80 90 100 110 120 RKLTVEDFNR ALRWSSVEAV CGYGSQEALP MRPAREGELY FPEDREVNLV ELALATNIPK 130 140 150 160 170 180 GCAETAVRVH VSYLDGKGNL APQGSVPSAV SSLTDDLLKY YHQVTRAVLG DDPQLMKVAL 190 200 210 220 230 240 QDLQTNSKIG ALLPYFVYVV SGVKSVSHDL EQLHRLLQVA RSLFRNPHLC LGPYVRCLVG 250 260 270 280 290 300 SVLYCVLEPL AASINPLNDH WTLRDGAALL LSHIFWTHGD LVSGLYQHIL LSLQKILADP 310 320 330 340 350 360 VRPLCCHYGA VVGLHALGWK AVERVLYPHL STYWTNLQAV LDDYSVSNAQ VKADGHKVYG 370 380 390 400 410 420 AILVAVERLL KMKAQAAEPN RGGPGGRGCR RLDDLPWDSL LFQESSSGGG AEPSFGSGLP 430 440 450 460 470 480 LPPGGAGPED PSLSVTLADI YRELYAFFGD SLATRFGTGQ PAPTAPRPPG DKKEPAAAPD 490 500 510 520 530 540 SVRKMPQLTA SAIVSPHGDE SPRGSGGGGP ASASGPAASE SRPLPRVHRA RGAPRQQGPG 550 560 570 580 590 600 TGTRDVFQKS RFAPRGAPHF RFIIAGRQAG RRCRGRLFQT AFPAPYGPSP ASRYVQKLPM 610 620 IGRTSRPARR WALSDYSLYL PL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Histone-like TAFs within the PCAF histone acetylase complex." Ogryzko V.V., Kotani T., Zhang X., Schiltz R.L., Howard T., Yang X.-J., Howard B.H., Qin J., Nakatani Y. Cell 94:35-44(1998) [PubMed: 9674425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 8-14; 62-70; 205-221 AND 577-593, SUBUNIT, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The TAFs in the HAT." Struhl K., Moqtaderi Z. Cell 94:1-4(1998) [PubMed: 9674419] [Abstract] Cited for: REVIEW, PCAF COMPLEX COMPOSITION.
[5]	"Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo." Martinez E., Palhan V.B., Tjernberg A., Lymar E.S., Gamper A.M., Kundu T.K., Chait B.T., Roeder R.G. Mol. Cell. Biol. 21:6782-6795(2001) [PubMed: 11564863] [Abstract] Cited for: IDENTIFICATION IN THE STAGA COMPLEX, SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[6]	"Novel subunits of the TATA binding protein free TAFII-containing transcription complex identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry following one-dimensional gel electrophoresis." Cavusoglu N., Brand M., Tora L., van Dorsselaer A. Proteomics 3:217-223(2003) [PubMed: 12601814] [Abstract] Cited for: IDENTIFICATION IN THE TFTC-HAT COMPLEX, MASS SPECTROMETRY.
[7]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85, MASS SPECTROMETRY. Tissue: Embryonic kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF069735 mRNA. Translation: AAC39905.1. AK314330 mRNA. Translation: BAG36977.1. CH471076 Genomic DNA. Translation: EAW74093.1.
IPI	IPI00007957.
RefSeq	NP_006464.1. NM_006473.3.
UniGene	Hs.714400.
3D structure databases
ProteinModelPortal	Q9Y6J9.
SMR	Q9Y6J9. Positions 8-73.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y6J9. 5 interactions.
MINT	MINT-1456123.
STRING	Q9Y6J9.
PTM databases
PhosphoSite	Q9Y6J9.
Polymorphism databases
DMDM	46577572.
Proteomic databases
PeptideAtlas	Q9Y6J9.
PRIDE	Q9Y6J9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000294168; ENSP00000294168; ENSG00000162227.
GeneID	10629.
KEGG	hsa:10629.
UCSC	uc001nvc.1. human.
Organism-specific databases
CTD	10629.
GeneCards	GC11P062538.
H-InvDB	HIX0009724.
HGNC	HGNC:17305. TAF6L.
MIM	602946. gene.
neXtProt	NX_Q9Y6J9.
PharmGKB	PA38224.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG13975.
GeneTree	ENSGT00530000063704.
HOGENOM	HBG716520.
HOVERGEN	HBG061566.
InParanoid	Q9Y6J9.
OMA	MPQLTAN.
OrthoDB	EOG498V0F.
PhylomeDB	Q9Y6J9.
Gene expression databases
ArrayExpress	Q9Y6J9.
Bgee	Q9Y6J9.
CleanEx	HS_TAF6L.
Genevestigator	Q9Y6J9.
GermOnline	ENSG00000162227. Homo sapiens.
Family and domain databases
InterPro	IPR011442. DUF1546. IPR009072. Histone-fold. IPR004823. TAF_TATA-bd. [Graphical view]
Gene3D	G3DSA:1.10.20.10. Histone-fold. 1 hit.
KO	K03131.
Pfam	PF07571. DUF1546. 1 hit. PF02969. TAF. 1 hit. [Graphical view]
SMART	SM00803. TAF. 1 hit. [Graphical view]
SUPFAM	SSF47113. Histone-fold. 1 hit.
ProtoNet	Search...
Other
NextBio	40385.
SOURCE	Search...

Entry information

Entry name

TAF6L_HUMAN

Accession

Primary (citable) accession number: Q9Y6J9
Secondary accession number(s): B2RAT0, Q96HA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	November 1, 1999
Last modified:	January 25, 2012
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.