Q9Y6I8 (PXMP4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxisomal membrane protein 4

Alternative name(s):
24 kDa peroxisomal intrinsic membrane protein

Gene names

Name:	PXMP4
Synonyms:	PMP24

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with PEX19. Ref.6 Ref.7
Subcellular location	Peroxisome membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Expressed in normal prostate epithelial cells, and androgen-sensitive prostate adenocarcinoma cells. Not expressed in androgen-insensitive prostate adenocarcinoma cells. Ref.8
Miscellaneous	Hypermethylation-mediated silencing of PXMP4 may be involved in prostate carcinogenesis. PXMP4 undergoes hypermethylation and silencing during the transition of prostate adenocarcinoma cells from androgen dependence to androgen independence.
Sequence similarities	Belongs to the peroxisomal membrane protein PXMP2/4 family.
Sequence caution	Isoform 2: The sequence AK297018 differs from that shown. Reason: a conflict in position 70

Ontologies

Keywords
Cellular component	Membrane Peroxisome
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW peroxisomal membrane Inferred from direct assay Ref.7. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y6I8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y6I8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     59-71: SLQEKLWAILQAT → RSTCTCCHASCLP
     72-212: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 212	211	Peroxisomal membrane protein 4		PRO_0000218932
Regions
	Transmembrane	97 – 117	21	Helical; Potential
	Transmembrane	153 – 173	21	Helical; Potential
Amino acid modifications
	Glycosylation	57	1	N-linked (GlcNAc...) Potential
	Glycosylation	206	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	59 – 71	13	SLQEK…ILQAT → RSTCTCCHASCLP in isoform 2.		VSP_041217
	Alternative sequence	72 – 212	141	Missing in isoform 2.		VSP_041218
	Natural variant	204	1	V → I. Ref.2 Ref.5 Corresponds to variant rs910397 [ dbSNP | Ensembl ].		VAR_015426

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: EE4DDA4B9BD16401	FASTA	212	24,264
	10 20 30 40 50 60 MAAPPQLRAL LVVVNALLRK RRYHAALAVL KGFRNGAVYG AKIRAPHALV MTFLFRNGSL 70 80 90 100 110 120 QEKLWAILQA TYIHSWNLAR FVFTYKGLRA LQSYIQGKTY PAHAFLAAFL GGILVFGENN 130 140 150 160 170 180 NINSQINMYL LSRVLFALSR LAVEKGYIPE PRWDPFPLLT AVVWGLVLWL FEYHRSTLQP 190 200 210 SLQSSMTYLY EDSNVWHDIS DFLVYNKSRP SN
	Isoform 2 [UniParc]. Checksum: B93B120C6EA15A9C	FASTA	71	7,763
	10 20 30 40 50 60 MAAPPQLRAL LVVVNALLRK RRYHAALAVL KGFRNGAVYG AKIRAPHALV MTFLFRNGRS 70 TCTCCHASCL P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a 24 kDa intrinsic membrane protein from mammalian peroxisomes." Reguenga C., Oliveira M.E.M., Gouveia A.M.M., Eckerskorn C., Sa-Miranda C., Azevedo J.E. Biochim. Biophys. Acta 1445:337-341(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION. Tissue: Liver.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ILE-204. Tissue: Brain.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ILE-204. Tissue: Lung.
[6]	"PEX19 binds multiple peroxisomal membrane proteins, is predominantly cytoplasmic, and is required for peroxisome membrane synthesis." Sacksteder K.A., Jones J.M., South S.T., Li X., Liu Y., Gould S.J. J. Cell Biol. 148:931-944(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PEX19.
[7]	"PEX19 is a predominantly cytosolic chaperone and import receptor for class 1 peroxisomal membrane proteins." Jones J.M., Morrell J.C., Gould S.J. J. Cell Biol. 164:57-67(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PEX19.
[8]	"PMP24, a gene identified by MSRF, undergoes DNA hypermethylation-associated gene silencing during cancer progression in an LNCaP model." Wu M., Ho S.M. Oncogene 23:250-259(2004) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, HYPERMETHYLATION, INVOLVEMENT IN PROSTATE CANCER.
[9]	"Methylation of a single intronic CpG mediates expression silencing of the PMP24 gene in prostate cancer." Zhang X., Wu M., Xiao H., Lee M.T., Levin L., Leung Y.K., Ho S.M. Prostate 70:765-776(2010) [PubMed] [Europe PMC] [Abstract] Cited for: HYPERMETHYLATION, INVOLVEMENT IN PROSTATE CANCER.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF072864 mRNA. Translation: AAD43195.1. AL136648 mRNA. Translation: CAB66583.1. AK297018 mRNA. No translation available. AL050349 Genomic DNA. Translation: CAC14086.1. AL050349 Genomic DNA. Translation: CAM28271.1. BC001147 mRNA. Translation: AAH01147.1.
IPI	IPI00218925. IPI00374131.
RefSeq	NP_009169.3. NM_007238.4. NP_899634.1. NM_183397.2.
UniGene	Hs.654857.
3D structure databases
ProteinModelPortal	Q9Y6I8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y6I8. 1 interaction.
STRING	9606.ENSP00000386385.
PTM databases
PhosphoSite	Q9Y6I8.
Polymorphism databases
DMDM	29840804.
Proteomic databases
PaxDb	Q9Y6I8.
PRIDE	Q9Y6I8.
Protocols and materials databases
DNASU	11264.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000344022; ENSP00000343071; ENSG00000101417. ENST00000409299; ENSP00000386385; ENSG00000101417.
GeneID	11264.
KEGG	hsa:11264.
UCSC	uc002wzv.3. human.
Organism-specific databases
CTD	11264.
GeneCards	GC20M032290.
HGNC	HGNC:15920. PXMP4.
HPA	HPA050077.
neXtProt	NX_Q9Y6I8.
PharmGKB	PA34061.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG79127.
HOGENOM	HOG000157771.
HOVERGEN	HBG053773.
InParanoid	Q9Y6I8.
KO	K13350.
OMA	YTHSRNL.
OrthoDB	EOG4CJVJ3.
PhylomeDB	Q9Y6I8.
Gene expression databases
ArrayExpress	Q9Y6I8.
Bgee	Q9Y6I8.
CleanEx	HS_PXMP4.
Genevestigator	Q9Y6I8.
GermOnline	ENSG00000101417. Homo sapiens.
Family and domain databases
InterPro	IPR019531. Pmp4. IPR003397. Tim17/Tim22/Tim23/PMP24. [Graphical view]
Pfam	PF02466. Tim17. 1 hit. [Graphical view]
PIRSF	PIRSF013674. PXMP4. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	11264.
NextBio	42865.

Entry information

Entry name

PXMP4_HUMAN

Accession

Primary (citable) accession number: Q9Y6I8
Secondary accession number(s): A2A2I7, Q9H0T4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 28, 2003
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 97 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.