Reviewed, UniProtKB/Swiss-Prot Q9Y6I4 (UBP3_HUMAN)

Names and origin

Protein names

Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 3
    EC=3.1.2.15
Alternative name(s):
    Ubiquitin thioesterase 3
    Ubiquitin-specific-processing protease 3
    Deubiquitinating enzyme 3

Gene names

Name:

USP3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.
Tissue specificity	Expressed in all tissues examined, with strongest expression in pancreas.
Sequence similarities	Belongs to the peptidase C19 family. Contains 1 UBP-type zinc finger.
Sequence caution	The sequence AAD42992.1 differs from that shown. Reason: Frameshift at positions 265, 274 and 299.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Polymorphism
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ubiquitin thiolesterase activity Inferred from electronic annotation. Source: EC ubiquitin-specific protease activity Ref.1 Traceable author statement. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 520	520	Ubiquitin carboxyl-terminal hydrolase 3		PRO_0000080619
Regions
	Zinc finger	27 – 104	78	UBP-type
Sites
	Active site	168	1	By similarity
	Active site	462	1	By similarity
	Active site	471	1	By similarity
Amino acid modifications
	Modified residue	141	1	Phosphothreonine Ref.5
Natural variations
	Natural variant	360	1	P → T: dbSNP rs34776764.		VAR_051521
Experimental info
	Sequence conflict	439	1	C → W in AAD42992. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9Y6I4-1 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: AC27FE9BD0438893	FASTA	520	58,897
	10 20 30 40 50 60 MECPHLSSSV CIAPDSAKFP NGSPSSWCCS VCRSNKSPWV CLTCSSVHCG RYVNGHAKKH 70 80 90 100 110 120 YEDAQVPLTN HKKSEKQDKV QHTVCMDCSS YSTYCYRCDD FVVNDTKLGL VQKVREHLQN 130 140 150 160 170 180 LENSAFTADR HKKRKLLENS TLNSKLLKVN GSTTAICATG LRNLGNTCFM NAILQSLSNI 190 200 210 220 230 240 EQFCCYFKEL PAVELRNGKT AGRRTYHTRS QGDNNVSLVE EFRKTLCALW QGSQTAFSPE 250 260 270 280 290 300 SLFYVVWKIM PNFRGYQQQD AHEFMRYLLD HLHLELQGGF NGVSRSAILQ ENSTLSASNK 310 320 330 340 350 360 CCINGASTVV TAIFGGILQN EVNCLICGTE SRKFDPFLDL SLDIPSQFRS KRSKNQENGP 370 380 390 400 410 420 VCSLRDCLRS FTDLEELDET ELYMCHKCKK KQKSTKKFWI QKLPKVLCLH LKRFHWTAYL 430 440 450 460 470 480 RNKVDTYVEF PLRGLDMKCY LLEPENSGPE SCLYDLAAVV VHHGSGVGSG HYTAYATHEG 490 500 510 520 RWFHFNDSTV TLTDEETVVK AKAYILFYVE HQAKAGSDKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization and chromosomal localization of USP3, a novel human ubiquitin-specific protease." Sloper-Mould K.E., Eyre H.J., Wang X.-W., Sutherland G.R., Baker R.T. J. Biol. Chem. 274:26878-26884(1999) [PubMed: 10480896] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye and Skin.
[5]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-141, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF073344 mRNA. Translation: AAD42992.1. Frameshift. BT007269 mRNA. Translation: AAP35933.1. CH471082 Genomic DNA. Translation: EAW77651.1. BC018113 mRNA. Translation: AAH18113.1. BC065300 mRNA. Translation: AAH65300.1. BC107137 mRNA. Translation: AAI07138.1. BC107138 mRNA. Translation: AAI07139.1.
IPI	IPI00002330.
RefSeq	NP_006528.2.
UniGene	Hs.458499
3D structure databases
HSSP	HSSP built from PDB template 1NB8 based on UniProtKB Q93009.
ModBase	Search...
Protein family/group databases
MEROPS	C19.026.
PTM databases
PhosphoSite	Q9Y6I4.
Proteomic databases
PRIDE	Q9Y6I4.
Genome annotation databases
Ensembl	ENSG00000140455. Homo sapiens. [Contig view]
GeneID	9960.
KEGG	hsa:9960.
Organism-specific databases
GeneCards	GC15P061582.
H-InvDB	HIX0012328.
HGNC	HGNC:12626. USP3.
MIM	604728. gene.
PharmGKB	PA27526.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q9Y6I4.
OMA	Q9Y6I4. LWQGSQT.
Enzyme and pathway databases
BRENDA	3.1.2.15. 247.
Gene expression databases
ArrayExpress	Q9Y6I4.
Bgee	Q9Y6I4.
CleanEx	HS_USP3.
GermOnline	ENSG00000140455. Homo sapiens.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR001607. Znf_UBP. [Graphical view]
Pfam	PF00443. UCH. 1 hit. PF02148. zf-UBP. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. PS50271. ZF_UBP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

UBP3_HUMAN

Accession

Primary (citable) accession number: Q9Y6I4
Secondary accession number(s): Q8WVD0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	September 2, 2008
Last modified:	June 16, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)