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Q9Y6D6

- BIG1_HUMAN

UniProt

Q9Y6D6 - BIG1_HUMAN

Protein

Brefeldin A-inhibited guanine nucleotide-exchange protein 1

Gene

ARFGEF1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (17 Oct 2006)
      Previous versions | rss
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    Functioni

    Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure; the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competetive RhoA binding. The function in the nucleus remains to be determined.5 Publications

    Enzyme regulationi

    Inhibited by brefeldin A.

    GO - Molecular functioni

    1. ARF guanyl-nucleotide exchange factor activity Source: RefGenome
    2. guanyl-nucleotide exchange factor activity Source: UniProtKB
    3. myosin binding Source: UniProtKB
    4. protein binding Source: UniProtKB
    5. protein kinase A regulatory subunit binding Source: UniProtKB

    GO - Biological processi

    1. endomembrane system organization Source: UniProtKB
    2. exocytosis Source: ProtInc
    3. Golgi organization Source: UniProtKB
    4. negative regulation of actin filament polymerization Source: UniProtKB
    5. negative regulation of Rho GTPase activity Source: UniProtKB
    6. positive regulation of GTPase activity Source: GOC
    7. positive regulation of protein glycosylation in Golgi Source: UniProtKB
    8. positive regulation of wound healing Source: UniProtKB
    9. protein transport Source: UniProtKB-KW
    10. regulation of ARF protein signal transduction Source: InterPro
    11. regulation of establishment of cell polarity Source: UniProtKB
    12. vesicle-mediated transport Source: RefGenome

    Keywords - Molecular functioni

    Guanine-nucleotide releasing factor

    Keywords - Biological processi

    Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Brefeldin A-inhibited guanine nucleotide-exchange protein 1
    Short name:
    Brefeldin A-inhibited GEP 1
    Alternative name(s):
    ADP-ribosylation factor guanine nucleotide-exchange factor 1
    p200 ARF guanine nucleotide exchange factor
    p200 ARF-GEP1
    Gene namesi
    Name:ARFGEF1
    Synonyms:ARFGEP1, BIG1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:15772. ARFGEF1.

    Subcellular locationi

    Cytoplasm. Cytoplasmperinuclear region. Golgi apparatus. Golgi apparatustrans-Golgi network By similarity. Nucleus. Nucleusnucleolus. Nucleus matrix. Membrane
    Note: Translocates from cytoplasm to membranes and nucleus upon cAMP treatment.

    GO - Cellular componenti

    1. cytoplasm Source: HPA
    2. cytosol Source: UniProtKB
    3. Golgi apparatus Source: HPA
    4. Golgi membrane Source: UniProtKB
    5. nuclear matrix Source: UniProtKB-SubCell
    6. nucleolus Source: UniProtKB
    7. nucleus Source: HPA
    8. perinuclear region of cytoplasm Source: UniProtKB
    9. small nuclear ribonucleoprotein complex Source: UniProtKB
    10. trans-Golgi network Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Golgi apparatus, Membrane, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi221 – 2211E → K: No effect on self-association. 1 Publication
    Mutagenesisi712 – 7143KPK → AAA: Inhibits nuclear localization.
    Mutagenesisi883 – 8831S → A: Abolishes cAMP-induced nuclear localization. 1 Publication
    Mutagenesisi883 – 8831S → D: No effect on cAMP-induced nuclear localization. 1 Publication

    Organism-specific databases

    PharmGKBiPA134908197.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 18491849Brefeldin A-inhibited guanine nucleotide-exchange protein 1PRO_0000120207Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1079 – 10791Phosphoserine2 Publications
    Modified residuei1569 – 15691Phosphoserine6 Publications

    Post-translational modificationi

    Phosphorylated. In vitro phosphorylated by PKA reducing its GEF activity and dephosphorylated by phosphatase PP1.6 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9Y6D6.
    PaxDbiQ9Y6D6.
    PeptideAtlasiQ9Y6D6.
    PRIDEiQ9Y6D6.

    PTM databases

    PhosphoSiteiQ9Y6D6.

    Expressioni

    Tissue specificityi

    Expressed in placenta, lung, heart, brain, kidney and pancreas.

    Gene expression databases

    ArrayExpressiQ9Y6D6.
    BgeeiQ9Y6D6.
    CleanExiHS_ARFGEF1.
    GenevestigatoriQ9Y6D6.

    Organism-specific databases

    HPAiHPA023399.
    HPA023822.

    Interactioni

    Subunit structurei

    Homodimer Probable. Interacts with BIG1; both proteins are probably part of the same or very similar macromolecular complexes. Interacts with FKBP2, DPY30, MYO9B, PRKAR1A, PRKAR2A, PPP1CC and PDE3A. Interacts with KANK1; however, colocalization cannot be experimentally confirmed. Interacts with NCL, FBL, NUP62 and U3 small nucleolar RNA.11 PublicationsCurated

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ARFGEF2Q9Y6D53EBI-1044254,EBI-2837511
    KIF21AQ7Z4S6-24EBI-1044254,EBI-6251716
    MYCBPQ994173EBI-1044254,EBI-716185
    MYO9BQ13459-22EBI-1044254,EBI-6251250

    Protein-protein interaction databases

    BioGridi115816. 12 interactions.
    DIPiDIP-29748N.
    IntActiQ9Y6D6. 10 interactions.
    MINTiMINT-1728435.
    STRINGi9606.ENSP00000262215.

    Structurei

    Secondary structure

    1
    1849
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi698 – 71114
    Helixi713 – 72210
    Helixi730 – 73910
    Helixi745 – 7528
    Helixi757 – 76812
    Helixi777 – 78610
    Helixi794 – 81017
    Helixi821 – 83818
    Helixi849 – 8557
    Beta strandi861 – 8644
    Helixi867 – 87913

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3LTLX-ray2.20A/B691-889[»]
    ProteinModelPortaliQ9Y6D6.
    SMRiQ9Y6D6. Positions 695-885.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9Y6D6.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini709 – 840132SEC7PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 224223DCB; DCB:DCB domain and DCB:HUS domain interactionAdd
    BLAST
    Regioni557 – 57721HUS; DCB:HUS domain interactionAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi711 – 7155Nuclear localization signal (NLS)

    Sequence similaritiesi

    Contains 1 SEC7 domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5307.
    HOGENOMiHOG000181045.
    HOVERGENiHBG004846.
    InParanoidiQ9Y6D6.
    OMAiILLMPCH.
    PhylomeDBiQ9Y6D6.
    TreeFamiTF300714.

    Family and domain databases

    Gene3Di1.10.1000.11. 1 hit.
    1.25.10.10. 4 hits.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR015403. DUF1981_Sec7_assoc.
    IPR023394. Sec7_alpha_orthog.
    IPR000904. Sec7_dom.
    [Graphical view]
    PfamiPF09324. DUF1981. 1 hit.
    PF01369. Sec7. 1 hit.
    [Graphical view]
    SMARTiSM00222. Sec7. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 3 hits.
    SSF48425. SSF48425. 1 hit.
    PROSITEiPS50190. SEC7. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Y6D6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYEGKKTKNM FLTRALEKIL ADKEVKKAHH SQLRKACEVA LEEIKAETEK     50
    QSPPHGEAKA GSSTLPPVKS KTNFIEADKY FLPFELACQS KCPRIVSTSL 100
    DCLQKLIAYG HLTGNAPDST TPGKKLIDRI IETICGCFQG PQTDEGVQLQ 150
    IIKALLTAVT SQHIEIHEGT VLQAVRTCYN IYLASKNLIN QTTAKATLTQ 200
    MLNVIFARME NQALQEAKQM EKERHRQHHH LLQSPVSHHE PESPQLRYLP 250
    PQTVDHISQE HEGDLDLHTN DVDKSLQDDT EPENGSDISS AENEQTEADQ 300
    ATAAETLSKN EVLYDGENHD CEEKPQDIVQ NIVEEMVNIV VGDMGEGTTI 350
    NASADGNIGT IEDGSDSENI QANGIPGTPI SVAYTPSLPD DRLSVSSNDT 400
    QESGNSSGPS PGAKFSHILQ KDAFLVFRSL CKLSMKPLSD GPPDPKSHEL 450
    RSKILSLQLL LSILQNAGPI FRTNEMFINA IKQYLCVALS KNGVSSVPEV 500
    FELSLSIFLT LLSNFKTHLK MQIEVFFKEI FLYILETSTS SFDHKWMVIQ 550
    TLTRICADAQ SVVDIYVNYD CDLNAANIFE RLVNDLSKIA QGRGSQELGM 600
    SNVQELSLRK KGLECLVSIL KCMVEWSKDQ YVNPNSQTTL GQEKPSEQEM 650
    SEIKHPETIN RYGSLNSLES TSSSGIGSYS TQMSGTDNPE QFEVLKQQKE 700
    IIEQGIDLFN KKPKRGIQYL QEQGMLGTTP EDIAQFLHQE ERLDSTQVGE 750
    FLGDNDKFNK EVMYAYVDQH DFSGKDFVSA LRMFLEGFRL PGEAQKIDRL 800
    MEKFAARYLE CNQGQTLFAS ADTAYVLAYS IIMLTTDLHS PQVKNKMTKE 850
    QYIKMNRGIN DSKDLPEEYL SAIYNEIAGK KISMKETKEL TIPTKSSKQN 900
    VASEKQRRLL YNLEMEQMAK TAKALMEAVS HVQAPFTSAT HLEHVRPMFK 950
    LAWTPFLAAF SVGLQDCDDT EVASLCLEGI RCAIRIACIF SIQLERDAYV 1000
    QALARFTLLT VSSGITEMKQ KNIDTIKTLI TVAHTDGNYL GNSWHEILKC 1050
    ISQLELAQLI GTGVKPRYIS GTVRGREGSL TGTKDQAPDE FVGLGLVGGN 1100
    VDWKQIASIQ ESIGETSSQS VVVAVDRIFT GSTRLDGNAI VDFVRWLCAV 1150
    SMDELLSTTH PRMFSLQKIV EISYYNMGRI RLQWSRIWEV IGDHFNKVGC 1200
    NPNEDVAIFA VDSLRQLSMK FLEKGELANF RFQKDFLRPF EHIMKRNRSP 1250
    TIRDMVVRCI AQMVNSQAAN IRSGWKNIFS VFHLAASDQD ESIVELAFQT 1300
    TGHIVTLVFE KHFPATIDSF QDAVKCLSEF ACNAAFPDTS MEAIRLIRHC 1350
    AKYVSDRPQA FKEYTSDDMN VAPEDRVWVR GWFPILFELS CIINRCKLDV 1400
    RTRGLTVMFE IMKTYGHTYE KHWWQDLFRI VFRIFDNMKL PEQQTEKAEW 1450
    MTTTCNHALY AICDVFTQYL EVLSDVLLDD IFAQLYWCVQ QDNEQLARSG 1500
    TNCLENVVIL NGEKFTLEIW DKTCNCTLDI FKTTIPHALL TWRPNSGETA 1550
    PPPPSPVSEK PLDTISQKSV DIHDSIQPRS VDNRPQAPLV SASAVNEEVS 1600
    KIKSTAKFPE QKLFAALLIK CVVQLELIQT IDNIVFFPAT SKKEDAENLA 1650
    AAQRDAVDFD VRVDTQDQGM YRFLTSQQLF KLLDCLLESH RFAKAFNSNN 1700
    EQRTALWKAG FKGKSKPNLL KQETSSLACG LRILFRMYMD ESRVSAWEEV 1750
    QQRLLNVCSE ALSYFLTLTS ESHREAWTNL LLLFLTKVLK ISDNRFKAHA 1800
    SFYYPLLCEI MQFDLIPELR AVLRRFFLRI GVVFQISQPP EQELGINKQ 1849
    Length:1,849
    Mass (Da):208,767
    Last modified:October 17, 2006 - v2
    Checksum:i1EB2547F28F63BCA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti233 – 2331Q → P in AAD38427. (PubMed:10212200)Curated
    Sequence conflicti620 – 6201L → S in AAD38427. (PubMed:10212200)Curated
    Sequence conflicti1055 – 10551E → K in AAD38427. (PubMed:10212200)Curated
    Sequence conflicti1590 – 15901V → A in BAA91912. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti273 – 2731D → Y.
    Corresponds to variant rs4321984 [ dbSNP | Ensembl ].
    VAR_028749
    Natural varianti316 – 3161G → E in a colorectal cancer sample; somatic mutation. 1 Publication
    VAR_036155

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF084520 mRNA. Translation: AAD38427.1.
    AF111162 mRNA. Translation: AAD43651.1.
    AK001788 mRNA. Translation: BAA91912.1.
    AL117446 mRNA. Translation: CAB55931.1.
    CCDSiCCDS6199.1.
    PIRiT17241.
    RefSeqiNP_006412.2. NM_006421.4.
    XP_005251191.1. XM_005251134.2.
    UniGeneiHs.656902.

    Genome annotation databases

    EnsembliENST00000262215; ENSP00000262215; ENSG00000066777.
    GeneIDi10565.
    KEGGihsa:10565.
    UCSCiuc003xxo.2. human.

    Polymorphism databases

    DMDMi116241267.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF084520 mRNA. Translation: AAD38427.1 .
    AF111162 mRNA. Translation: AAD43651.1 .
    AK001788 mRNA. Translation: BAA91912.1 .
    AL117446 mRNA. Translation: CAB55931.1 .
    CCDSi CCDS6199.1.
    PIRi T17241.
    RefSeqi NP_006412.2. NM_006421.4.
    XP_005251191.1. XM_005251134.2.
    UniGenei Hs.656902.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3LTL X-ray 2.20 A/B 691-889 [» ]
    ProteinModelPortali Q9Y6D6.
    SMRi Q9Y6D6. Positions 695-885.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115816. 12 interactions.
    DIPi DIP-29748N.
    IntActi Q9Y6D6. 10 interactions.
    MINTi MINT-1728435.
    STRINGi 9606.ENSP00000262215.

    PTM databases

    PhosphoSitei Q9Y6D6.

    Polymorphism databases

    DMDMi 116241267.

    Proteomic databases

    MaxQBi Q9Y6D6.
    PaxDbi Q9Y6D6.
    PeptideAtlasi Q9Y6D6.
    PRIDEi Q9Y6D6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000262215 ; ENSP00000262215 ; ENSG00000066777 .
    GeneIDi 10565.
    KEGGi hsa:10565.
    UCSCi uc003xxo.2. human.

    Organism-specific databases

    CTDi 10565.
    GeneCardsi GC08M068085.
    HGNCi HGNC:15772. ARFGEF1.
    HPAi HPA023399.
    HPA023822.
    MIMi 604141. gene.
    neXtProti NX_Q9Y6D6.
    PharmGKBi PA134908197.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5307.
    HOGENOMi HOG000181045.
    HOVERGENi HBG004846.
    InParanoidi Q9Y6D6.
    OMAi ILLMPCH.
    PhylomeDBi Q9Y6D6.
    TreeFami TF300714.

    Miscellaneous databases

    ChiTaRSi ARFGEF1. human.
    EvolutionaryTracei Q9Y6D6.
    GeneWikii ARFGEF1.
    GenomeRNAii 10565.
    NextBioi 40097.
    PROi Q9Y6D6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Y6D6.
    Bgeei Q9Y6D6.
    CleanExi HS_ARFGEF1.
    Genevestigatori Q9Y6D6.

    Family and domain databases

    Gene3Di 1.10.1000.11. 1 hit.
    1.25.10.10. 4 hits.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR015403. DUF1981_Sec7_assoc.
    IPR023394. Sec7_alpha_orthog.
    IPR000904. Sec7_dom.
    [Graphical view ]
    Pfami PF09324. DUF1981. 1 hit.
    PF01369. Sec7. 1 hit.
    [Graphical view ]
    SMARTi SM00222. Sec7. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 3 hits.
    SSF48425. SSF48425. 1 hit.
    PROSITEi PS50190. SEC7. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Purification and cloning of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors."
      Togawa A., Morinaga N., Ogasawara M., Moss J., Vaughan M.
      J. Biol. Chem. 274:12308-12315(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "p200 ARF-GEP1: a Golgi-localized guanine nucleotide exchange protein whose Sec7 domain is targeted by the drug brefeldin A."
      Mansour S.J., Skaug J., Zhao X.-H., Giordano J., Scherer S.W., Melancon P.
      Proc. Natl. Acad. Sci. U.S.A. 96:7968-7973(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1341-1849.
      Tissue: Ovarian carcinoma.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1637-1849.
      Tissue: Testis.
    5. "Identification and localization of two brefeldin A-inhibited guanine nucleotide-exchange proteins for ADP-ribosylation factors in a macromolecular complex."
      Yamaji R., Adamik R., Takeda K., Togawa A., Pacheco-Rodriguez G., Ferrans V.J., Moss J., Vaughan M.
      Proc. Natl. Acad. Sci. U.S.A. 97:2567-2572(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH BIG2.
    6. "Protein kinase A-anchoring (AKAP) domains in brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2)."
      Li H., Adamik R., Pacheco-Rodriguez G., Moss J., Vaughan M.
      Proc. Natl. Acad. Sci. U.S.A. 100:1627-1632(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PRKAR1A AND PRKAR2A, SUBCELLULAR LOCATION.
    7. "Interaction of FK506-binding protein 13 with brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1): effects of FK506."
      Padilla P.I., Chang M.J., Pacheco-Rodriguez G., Adamik R., Moss J., Vaughan M.
      Proc. Natl. Acad. Sci. U.S.A. 100:2322-2327(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FKBP2.
    8. "Nuclear localization and molecular partners of BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors."
      Padilla P.I., Pacheco-Rodriguez G., Moss J., Vaughan M.
      Proc. Natl. Acad. Sci. U.S.A. 101:2752-2757(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH NCL AND NUP62.
    9. "BIG1 is a binding partner of myosin IXb and regulates its Rho-GTPase activating protein activity."
      Saeki N., Tokuo H., Ikebe M.
      J. Biol. Chem. 280:10128-10134(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MYO9B.
    10. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1569, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    11. "Effect of protein kinase A on accumulation of brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1) in HepG2 cell nuclei."
      Citterio C., Jones H.D., Pacheco-Rodriguez G., Islam A., Moss J., Vaughan M.
      Proc. Natl. Acad. Sci. U.S.A. 103:2683-2688(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF 712-LYS--LYS-714 AND SER-883.
    12. "Interactions between conserved domains within homodimers in the BIG1, BIG2, and GBF1 Arf guanine nucleotide exchange factors."
      Ramaen O., Joubert A., Simister P., Belgareh-Touze N., Olivares-Sanchez M.C., Zeeh J.C., Chantalat S., Golinelli-Cohen M.P., Jackson C.L., Biou V., Cherfils J.
      J. Biol. Chem. 282:28834-28842(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, MUTAGENESIS OF GLU-221.
    13. "BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein, is required for correct glycosylation and function of integrin beta1."
      Shen X., Hong M.S., Moss J., Vaughan M.
      Proc. Natl. Acad. Sci. U.S.A. 104:1230-1235(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    14. "Regulation of brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1) and BIG2 activity via PKA and protein phosphatase 1gamma."
      Kuroda F., Moss J., Vaughan M.
      Proc. Natl. Acad. Sci. U.S.A. 104:3201-3206(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION, INTERACTION WITH PPP1CC.
    15. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1569, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    16. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1569, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    17. "Association of guanine nucleotide-exchange protein BIG1 in HepG2 cell nuclei with nucleolin, U3 snoRNA, and fibrillarin."
      Padilla P.I., Uhart M., Pacheco-Rodriguez G., Peculis B.A., Moss J., Vaughan M.
      Proc. Natl. Acad. Sci. U.S.A. 105:3357-3361(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NCL; FBL; NUP62 AND U3 SMALL NUCLEOLAR RNA, IDENTIFICATION IN SMALL NUCLEAR RIBONUCLEOPROTEIN COMPLEX.
    18. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    19. "A role of histone H3 lysine 4 methyltransferase components in endosomal trafficking."
      Xu Z., Gong Q., Xia B., Groves B., Zimmermann M., Mugler C., Mu D., Matsumoto B., Seaman M., Ma D.
      J. Cell Biol. 186:343-353(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DPY30.
    20. "Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity."
      Puxeddu E., Uhart M., Li C.C., Ahmad F., Pacheco-Rodriguez G., Manganiello V.C., Moss J., Vaughan M.
      Proc. Natl. Acad. Sci. U.S.A. 106:6158-6163(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PDE3A.
    21. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    22. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1569, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    23. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    24. "Effects of brefeldin A-inhibited guanine nucleotide-exchange (BIG) 1 and KANK1 proteins on cell polarity and directed migration during wound healing."
      Li C.C., Kuo J.C., Waterman C.M., Kiyama R., Moss J., Vaughan M.
      Proc. Natl. Acad. Sci. U.S.A. 108:19228-19233(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH KANK1.
    25. "Specific functions of BIG1 and BIG2 in endomembrane organization."
      Boal F., Stephens D.J.
      PLoS ONE 5:E9898-E9898(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    26. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1079 AND SER-1569, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    27. Cited for: VARIANT [LARGE SCALE ANALYSIS] GLU-316.

    Entry informationi

    Entry nameiBIG1_HUMAN
    AccessioniPrimary (citable) accession number: Q9Y6D6
    Secondary accession number(s): Q9NV46, Q9UFV2, Q9UNL0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3