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Q9Y6D6

- BIG1_HUMAN

UniProt

Q9Y6D6 - BIG1_HUMAN

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Protein

Brefeldin A-inhibited guanine nucleotide-exchange protein 1

Gene

ARFGEF1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure; the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competetive RhoA binding. The function in the nucleus remains to be determined.5 Publications

Enzyme regulationi

Inhibited by brefeldin A.

GO - Molecular functioni

  1. ARF guanyl-nucleotide exchange factor activity Source: RefGenome
  2. guanyl-nucleotide exchange factor activity Source: UniProtKB
  3. myosin binding Source: UniProtKB
  4. protein kinase A regulatory subunit binding Source: UniProtKB

GO - Biological processi

  1. endomembrane system organization Source: UniProtKB
  2. exocytosis Source: ProtInc
  3. Golgi organization Source: UniProtKB
  4. negative regulation of actin filament polymerization Source: UniProtKB
  5. negative regulation of Rho GTPase activity Source: UniProtKB
  6. positive regulation of GTPase activity Source: GOC
  7. positive regulation of protein glycosylation in Golgi Source: UniProtKB
  8. positive regulation of wound healing Source: UniProtKB
  9. protein transport Source: UniProtKB-KW
  10. regulation of ARF protein signal transduction Source: InterPro
  11. regulation of establishment of cell polarity Source: UniProtKB
  12. vesicle-mediated transport Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Brefeldin A-inhibited guanine nucleotide-exchange protein 1
Short name:
Brefeldin A-inhibited GEP 1
Alternative name(s):
ADP-ribosylation factor guanine nucleotide-exchange factor 1
p200 ARF guanine nucleotide exchange factor
p200 ARF-GEP1
Gene namesi
Name:ARFGEF1
Synonyms:ARFGEP1, BIG1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 8

Organism-specific databases

HGNCiHGNC:15772. ARFGEF1.

Subcellular locationi

Cytoplasm. Cytoplasmperinuclear region. Golgi apparatus. Golgi apparatustrans-Golgi network By similarity. Nucleus. Nucleusnucleolus. Nucleus matrix. Membrane
Note: Translocates from cytoplasm to membranes and nucleus upon cAMP treatment.

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. cytosol Source: UniProtKB
  3. Golgi apparatus Source: HPA
  4. Golgi membrane Source: UniProtKB
  5. nucleolus Source: UniProtKB
  6. nucleus Source: HPA
  7. perinuclear region of cytoplasm Source: UniProtKB
  8. small nuclear ribonucleoprotein complex Source: UniProtKB
  9. trans-Golgi network Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi221 – 2211E → K: No effect on self-association. 1 Publication
Mutagenesisi712 – 7143KPK → AAA: Inhibits nuclear localization. 1 Publication
Mutagenesisi883 – 8831S → A: Abolishes cAMP-induced nuclear localization. 1 Publication
Mutagenesisi883 – 8831S → D: No effect on cAMP-induced nuclear localization. 1 Publication

Organism-specific databases

PharmGKBiPA134908197.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18491849Brefeldin A-inhibited guanine nucleotide-exchange protein 1PRO_0000120207Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1079 – 10791Phosphoserine1 Publication
Modified residuei1569 – 15691Phosphoserine5 Publications

Post-translational modificationi

Phosphorylated. In vitro phosphorylated by PKA reducing its GEF activity and dephosphorylated by phosphatase PP1.6 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Y6D6.
PaxDbiQ9Y6D6.
PeptideAtlasiQ9Y6D6.
PRIDEiQ9Y6D6.

PTM databases

PhosphoSiteiQ9Y6D6.

Expressioni

Tissue specificityi

Expressed in placenta, lung, heart, brain, kidney and pancreas.

Gene expression databases

BgeeiQ9Y6D6.
CleanExiHS_ARFGEF1.
ExpressionAtlasiQ9Y6D6. baseline and differential.
GenevestigatoriQ9Y6D6.

Organism-specific databases

HPAiHPA023399.
HPA023822.

Interactioni

Subunit structurei

Homodimer (Probable). Interacts with BIG1; both proteins are probably part of the same or very similar macromolecular complexes. Interacts with FKBP2, DPY30, MYO9B, PRKAR1A, PRKAR2A, PPP1CC and PDE3A. Interacts with KANK1; however, colocalization cannot be experimentally confirmed. Interacts with NCL, FBL, NUP62 and U3 small nucleolar RNA.11 PublicationsCurated

Binary interactionsi

WithEntry#Exp.IntActNotes
ARFGEF2Q9Y6D53EBI-1044254,EBI-2837511
KIF21AQ7Z4S6-24EBI-1044254,EBI-6251716
MYCBPQ994173EBI-1044254,EBI-716185
MYO9BQ13459-22EBI-1044254,EBI-6251250

Protein-protein interaction databases

BioGridi115816. 21 interactions.
DIPiDIP-29748N.
IntActiQ9Y6D6. 10 interactions.
MINTiMINT-1728435.
STRINGi9606.ENSP00000262215.

Structurei

Secondary structure

1
1849
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi698 – 71114
Helixi713 – 72210
Helixi730 – 73910
Helixi745 – 7528
Helixi757 – 76812
Helixi777 – 78610
Helixi794 – 81017
Helixi821 – 83818
Helixi849 – 8557
Beta strandi861 – 8644
Helixi867 – 87913

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3LTLX-ray2.20A/B691-889[»]
ProteinModelPortaliQ9Y6D6.
SMRiQ9Y6D6. Positions 695-885.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y6D6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini709 – 840132SEC7PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 224223DCB; DCB:DCB domain and DCB:HUS domain interactionAdd
BLAST
Regioni557 – 57721HUS; DCB:HUS domain interactionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi711 – 7155Nuclear localization signal (NLS)

Sequence similaritiesi

Contains 1 SEC7 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5307.
GeneTreeiENSGT00760000119036.
HOGENOMiHOG000181045.
HOVERGENiHBG004846.
InParanoidiQ9Y6D6.
KOiK18442.
OMAiILLMPCH.
PhylomeDBiQ9Y6D6.
TreeFamiTF300714.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
1.25.10.10. 4 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR015403. DUF1981_Sec7_assoc.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PfamiPF09324. DUF1981. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view]
SMARTiSM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
SSF48425. SSF48425. 1 hit.
PROSITEiPS50190. SEC7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Y6D6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYEGKKTKNM FLTRALEKIL ADKEVKKAHH SQLRKACEVA LEEIKAETEK
60 70 80 90 100
QSPPHGEAKA GSSTLPPVKS KTNFIEADKY FLPFELACQS KCPRIVSTSL
110 120 130 140 150
DCLQKLIAYG HLTGNAPDST TPGKKLIDRI IETICGCFQG PQTDEGVQLQ
160 170 180 190 200
IIKALLTAVT SQHIEIHEGT VLQAVRTCYN IYLASKNLIN QTTAKATLTQ
210 220 230 240 250
MLNVIFARME NQALQEAKQM EKERHRQHHH LLQSPVSHHE PESPQLRYLP
260 270 280 290 300
PQTVDHISQE HEGDLDLHTN DVDKSLQDDT EPENGSDISS AENEQTEADQ
310 320 330 340 350
ATAAETLSKN EVLYDGENHD CEEKPQDIVQ NIVEEMVNIV VGDMGEGTTI
360 370 380 390 400
NASADGNIGT IEDGSDSENI QANGIPGTPI SVAYTPSLPD DRLSVSSNDT
410 420 430 440 450
QESGNSSGPS PGAKFSHILQ KDAFLVFRSL CKLSMKPLSD GPPDPKSHEL
460 470 480 490 500
RSKILSLQLL LSILQNAGPI FRTNEMFINA IKQYLCVALS KNGVSSVPEV
510 520 530 540 550
FELSLSIFLT LLSNFKTHLK MQIEVFFKEI FLYILETSTS SFDHKWMVIQ
560 570 580 590 600
TLTRICADAQ SVVDIYVNYD CDLNAANIFE RLVNDLSKIA QGRGSQELGM
610 620 630 640 650
SNVQELSLRK KGLECLVSIL KCMVEWSKDQ YVNPNSQTTL GQEKPSEQEM
660 670 680 690 700
SEIKHPETIN RYGSLNSLES TSSSGIGSYS TQMSGTDNPE QFEVLKQQKE
710 720 730 740 750
IIEQGIDLFN KKPKRGIQYL QEQGMLGTTP EDIAQFLHQE ERLDSTQVGE
760 770 780 790 800
FLGDNDKFNK EVMYAYVDQH DFSGKDFVSA LRMFLEGFRL PGEAQKIDRL
810 820 830 840 850
MEKFAARYLE CNQGQTLFAS ADTAYVLAYS IIMLTTDLHS PQVKNKMTKE
860 870 880 890 900
QYIKMNRGIN DSKDLPEEYL SAIYNEIAGK KISMKETKEL TIPTKSSKQN
910 920 930 940 950
VASEKQRRLL YNLEMEQMAK TAKALMEAVS HVQAPFTSAT HLEHVRPMFK
960 970 980 990 1000
LAWTPFLAAF SVGLQDCDDT EVASLCLEGI RCAIRIACIF SIQLERDAYV
1010 1020 1030 1040 1050
QALARFTLLT VSSGITEMKQ KNIDTIKTLI TVAHTDGNYL GNSWHEILKC
1060 1070 1080 1090 1100
ISQLELAQLI GTGVKPRYIS GTVRGREGSL TGTKDQAPDE FVGLGLVGGN
1110 1120 1130 1140 1150
VDWKQIASIQ ESIGETSSQS VVVAVDRIFT GSTRLDGNAI VDFVRWLCAV
1160 1170 1180 1190 1200
SMDELLSTTH PRMFSLQKIV EISYYNMGRI RLQWSRIWEV IGDHFNKVGC
1210 1220 1230 1240 1250
NPNEDVAIFA VDSLRQLSMK FLEKGELANF RFQKDFLRPF EHIMKRNRSP
1260 1270 1280 1290 1300
TIRDMVVRCI AQMVNSQAAN IRSGWKNIFS VFHLAASDQD ESIVELAFQT
1310 1320 1330 1340 1350
TGHIVTLVFE KHFPATIDSF QDAVKCLSEF ACNAAFPDTS MEAIRLIRHC
1360 1370 1380 1390 1400
AKYVSDRPQA FKEYTSDDMN VAPEDRVWVR GWFPILFELS CIINRCKLDV
1410 1420 1430 1440 1450
RTRGLTVMFE IMKTYGHTYE KHWWQDLFRI VFRIFDNMKL PEQQTEKAEW
1460 1470 1480 1490 1500
MTTTCNHALY AICDVFTQYL EVLSDVLLDD IFAQLYWCVQ QDNEQLARSG
1510 1520 1530 1540 1550
TNCLENVVIL NGEKFTLEIW DKTCNCTLDI FKTTIPHALL TWRPNSGETA
1560 1570 1580 1590 1600
PPPPSPVSEK PLDTISQKSV DIHDSIQPRS VDNRPQAPLV SASAVNEEVS
1610 1620 1630 1640 1650
KIKSTAKFPE QKLFAALLIK CVVQLELIQT IDNIVFFPAT SKKEDAENLA
1660 1670 1680 1690 1700
AAQRDAVDFD VRVDTQDQGM YRFLTSQQLF KLLDCLLESH RFAKAFNSNN
1710 1720 1730 1740 1750
EQRTALWKAG FKGKSKPNLL KQETSSLACG LRILFRMYMD ESRVSAWEEV
1760 1770 1780 1790 1800
QQRLLNVCSE ALSYFLTLTS ESHREAWTNL LLLFLTKVLK ISDNRFKAHA
1810 1820 1830 1840
SFYYPLLCEI MQFDLIPELR AVLRRFFLRI GVVFQISQPP EQELGINKQ
Length:1,849
Mass (Da):208,767
Last modified:October 17, 2006 - v2
Checksum:i1EB2547F28F63BCA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti233 – 2331Q → P in AAD38427. (PubMed:10212200)Curated
Sequence conflicti620 – 6201L → S in AAD38427. (PubMed:10212200)Curated
Sequence conflicti1055 – 10551E → K in AAD38427. (PubMed:10212200)Curated
Sequence conflicti1590 – 15901V → A in BAA91912. (PubMed:14702039)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti273 – 2731D → Y.
Corresponds to variant rs4321984 [ dbSNP | Ensembl ].
VAR_028749
Natural varianti316 – 3161G → E in a colorectal cancer sample; somatic mutation. 1 Publication
VAR_036155

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF084520 mRNA. Translation: AAD38427.1.
AF111162 mRNA. Translation: AAD43651.1.
AK001788 mRNA. Translation: BAA91912.1.
AL117446 mRNA. Translation: CAB55931.1.
CCDSiCCDS6199.1.
PIRiT17241.
RefSeqiNP_006412.2. NM_006421.4.
XP_005251191.1. XM_005251134.2.
UniGeneiHs.656902.

Genome annotation databases

EnsembliENST00000262215; ENSP00000262215; ENSG00000066777.
GeneIDi10565.
KEGGihsa:10565.
UCSCiuc003xxo.2. human.

Polymorphism databases

DMDMi116241267.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF084520 mRNA. Translation: AAD38427.1 .
AF111162 mRNA. Translation: AAD43651.1 .
AK001788 mRNA. Translation: BAA91912.1 .
AL117446 mRNA. Translation: CAB55931.1 .
CCDSi CCDS6199.1.
PIRi T17241.
RefSeqi NP_006412.2. NM_006421.4.
XP_005251191.1. XM_005251134.2.
UniGenei Hs.656902.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3LTL X-ray 2.20 A/B 691-889 [» ]
ProteinModelPortali Q9Y6D6.
SMRi Q9Y6D6. Positions 695-885.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 115816. 21 interactions.
DIPi DIP-29748N.
IntActi Q9Y6D6. 10 interactions.
MINTi MINT-1728435.
STRINGi 9606.ENSP00000262215.

PTM databases

PhosphoSitei Q9Y6D6.

Polymorphism databases

DMDMi 116241267.

Proteomic databases

MaxQBi Q9Y6D6.
PaxDbi Q9Y6D6.
PeptideAtlasi Q9Y6D6.
PRIDEi Q9Y6D6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000262215 ; ENSP00000262215 ; ENSG00000066777 .
GeneIDi 10565.
KEGGi hsa:10565.
UCSCi uc003xxo.2. human.

Organism-specific databases

CTDi 10565.
GeneCardsi GC08M068085.
HGNCi HGNC:15772. ARFGEF1.
HPAi HPA023399.
HPA023822.
MIMi 604141. gene.
neXtProti NX_Q9Y6D6.
PharmGKBi PA134908197.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5307.
GeneTreei ENSGT00760000119036.
HOGENOMi HOG000181045.
HOVERGENi HBG004846.
InParanoidi Q9Y6D6.
KOi K18442.
OMAi ILLMPCH.
PhylomeDBi Q9Y6D6.
TreeFami TF300714.

Miscellaneous databases

ChiTaRSi ARFGEF1. human.
EvolutionaryTracei Q9Y6D6.
GeneWikii ARFGEF1.
GenomeRNAii 10565.
NextBioi 40097.
PROi Q9Y6D6.
SOURCEi Search...

Gene expression databases

Bgeei Q9Y6D6.
CleanExi HS_ARFGEF1.
ExpressionAtlasi Q9Y6D6. baseline and differential.
Genevestigatori Q9Y6D6.

Family and domain databases

Gene3Di 1.10.1000.11. 1 hit.
1.25.10.10. 4 hits.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR015403. DUF1981_Sec7_assoc.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view ]
Pfami PF09324. DUF1981. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view ]
SMARTi SM00222. Sec7. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 3 hits.
SSF48425. SSF48425. 1 hit.
PROSITEi PS50190. SEC7. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Purification and cloning of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors."
    Togawa A., Morinaga N., Ogasawara M., Moss J., Vaughan M.
    J. Biol. Chem. 274:12308-12315(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "p200 ARF-GEP1: a Golgi-localized guanine nucleotide exchange protein whose Sec7 domain is targeted by the drug brefeldin A."
    Mansour S.J., Skaug J., Zhao X.-H., Giordano J., Scherer S.W., Melancon P.
    Proc. Natl. Acad. Sci. U.S.A. 96:7968-7973(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1341-1849.
    Tissue: Ovarian carcinoma.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1637-1849.
    Tissue: Testis.
  5. "Identification and localization of two brefeldin A-inhibited guanine nucleotide-exchange proteins for ADP-ribosylation factors in a macromolecular complex."
    Yamaji R., Adamik R., Takeda K., Togawa A., Pacheco-Rodriguez G., Ferrans V.J., Moss J., Vaughan M.
    Proc. Natl. Acad. Sci. U.S.A. 97:2567-2572(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH BIG2.
  6. "Protein kinase A-anchoring (AKAP) domains in brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2)."
    Li H., Adamik R., Pacheco-Rodriguez G., Moss J., Vaughan M.
    Proc. Natl. Acad. Sci. U.S.A. 100:1627-1632(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PRKAR1A AND PRKAR2A, SUBCELLULAR LOCATION.
  7. "Interaction of FK506-binding protein 13 with brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1): effects of FK506."
    Padilla P.I., Chang M.J., Pacheco-Rodriguez G., Adamik R., Moss J., Vaughan M.
    Proc. Natl. Acad. Sci. U.S.A. 100:2322-2327(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FKBP2.
  8. "Nuclear localization and molecular partners of BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors."
    Padilla P.I., Pacheco-Rodriguez G., Moss J., Vaughan M.
    Proc. Natl. Acad. Sci. U.S.A. 101:2752-2757(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH NCL AND NUP62.
  9. "BIG1 is a binding partner of myosin IXb and regulates its Rho-GTPase activating protein activity."
    Saeki N., Tokuo H., Ikebe M.
    J. Biol. Chem. 280:10128-10134(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MYO9B.
  10. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1569, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  11. "Effect of protein kinase A on accumulation of brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1) in HepG2 cell nuclei."
    Citterio C., Jones H.D., Pacheco-Rodriguez G., Islam A., Moss J., Vaughan M.
    Proc. Natl. Acad. Sci. U.S.A. 103:2683-2688(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF 712-LYS--LYS-714 AND SER-883.
  12. "Interactions between conserved domains within homodimers in the BIG1, BIG2, and GBF1 Arf guanine nucleotide exchange factors."
    Ramaen O., Joubert A., Simister P., Belgareh-Touze N., Olivares-Sanchez M.C., Zeeh J.C., Chantalat S., Golinelli-Cohen M.P., Jackson C.L., Biou V., Cherfils J.
    J. Biol. Chem. 282:28834-28842(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, MUTAGENESIS OF GLU-221.
  13. "BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein, is required for correct glycosylation and function of integrin beta1."
    Shen X., Hong M.S., Moss J., Vaughan M.
    Proc. Natl. Acad. Sci. U.S.A. 104:1230-1235(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. "Regulation of brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1) and BIG2 activity via PKA and protein phosphatase 1gamma."
    Kuroda F., Moss J., Vaughan M.
    Proc. Natl. Acad. Sci. U.S.A. 104:3201-3206(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION, INTERACTION WITH PPP1CC.
  15. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1569, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  16. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1569, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  17. "Association of guanine nucleotide-exchange protein BIG1 in HepG2 cell nuclei with nucleolin, U3 snoRNA, and fibrillarin."
    Padilla P.I., Uhart M., Pacheco-Rodriguez G., Peculis B.A., Moss J., Vaughan M.
    Proc. Natl. Acad. Sci. U.S.A. 105:3357-3361(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NCL; FBL; NUP62 AND U3 SMALL NUCLEOLAR RNA, IDENTIFICATION IN SMALL NUCLEAR RIBONUCLEOPROTEIN COMPLEX.
  18. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  19. "A role of histone H3 lysine 4 methyltransferase components in endosomal trafficking."
    Xu Z., Gong Q., Xia B., Groves B., Zimmermann M., Mugler C., Mu D., Matsumoto B., Seaman M., Ma D.
    J. Cell Biol. 186:343-353(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DPY30.
  20. "Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity."
    Puxeddu E., Uhart M., Li C.C., Ahmad F., Pacheco-Rodriguez G., Manganiello V.C., Moss J., Vaughan M.
    Proc. Natl. Acad. Sci. U.S.A. 106:6158-6163(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PDE3A.
  21. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  22. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1569, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  23. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  24. "Effects of brefeldin A-inhibited guanine nucleotide-exchange (BIG) 1 and KANK1 proteins on cell polarity and directed migration during wound healing."
    Li C.C., Kuo J.C., Waterman C.M., Kiyama R., Moss J., Vaughan M.
    Proc. Natl. Acad. Sci. U.S.A. 108:19228-19233(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH KANK1.
  25. "Specific functions of BIG1 and BIG2 in endomembrane organization."
    Boal F., Stephens D.J.
    PLoS ONE 5:E9898-E9898(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  26. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1079 AND SER-1569, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  27. Cited for: VARIANT [LARGE SCALE ANALYSIS] GLU-316.

Entry informationi

Entry nameiBIG1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6D6
Secondary accession number(s): Q9NV46, Q9UFV2, Q9UNL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: October 17, 2006
Last modified: October 29, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

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