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Protein

Selenoprotein K

Gene

SELK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for Ca2+ flux in immune cells and plays a role in T-cell proliferation and in T-cell and neutrophil migration. Involved in endoplasmic reticulum-associated degradation (ERAD) of soluble glycosylated proteins. Required for palmitoylation and cell surface expression of CD36 and involved in macrophage uptake of low-density lipoprotein and in foam cell formation. Plays a role in protection of cells from ER stress-induced apoptosis. Protects cells from oxidative stress when overexpressed in cardiomyocytes.3 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct

GO - Biological processi

  • calcium ion transport Source: UniProtKB-KW
  • intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: UniProtKB
  • response to oxidative stress Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Selenoprotein K
Short name:
SelK
Gene namesi
Name:SELK
ORF Names:HSPC030, HSPC297
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei20 – 4223HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum membrane Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Polymorphism and mutation databases

BioMutaiSELK.
DMDMi190352222.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 9493Selenoprotein KPRO_0000097669Add
BLAST

Post-translational modificationi

Cleaved by CAPN2/m-calpain in resting macrophages but not in activated macrophages. Macrophage activation up-regulates expression of the calpain inhibitor CAST/calpastatin, resulting in inhibition of CAPN2 activity (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei81 – 822Cleavage; by CAPN2By similarity

Proteomic databases

EPDiQ9Y6D0.
MaxQBiQ9Y6D0.
PaxDbiQ9Y6D0.
PRIDEiQ9Y6D0.

PTM databases

iPTMnetiQ9Y6D0.
PhosphoSiteiQ9Y6D0.

Expressioni

Tissue specificityi

Highly expressed in heart.1 Publication

Inductioni

By ER stress (at protein level). Displays a slow increase with a lag phase of 6 hours but exhibits a dramatic increase after incubation with ER stress agents for more than 12 hours with maximum induction at 24 hours. Also induced by accumulation of misfolded proteins in the ER.2 Publications

Organism-specific databases

HPAiHPA008196.

Interactioni

Subunit structurei

Interacts with DERL1, DERL2, DERL3 and VIMP/SELS. The SELK-VIMP complex interacts with VCP. Part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, VIMP, STT3A AND VCP.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-9679163,EBI-9679163

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi121841. 3 interactions.
IntActiQ9Y6D0. 1 interaction.
STRINGi9606.ENSP00000418813.

Structurei

3D structure databases

ProteinModelPortaliQ9Y6D0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the selenoprotein K family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J0AZ. Eukaryota.
ENOG410XWKI. LUCA.
GeneTreeiENSGT00390000016119.
HOGENOMiHOG000013002.
HOVERGENiHBG108467.
InParanoidiQ9Y6D0.
OMAiMGRINHW.
PhylomeDBiQ9Y6D0.

Family and domain databases

InterProiIPR024491. Se_SelK/SelG.
[Graphical view]
PfamiPF10961. DUF2763. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Y6D0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVYISNGQVL DSRSQSPWRL SLITDFFWGI AEFVVLFFKT LLQQDVKKRR
60 70 80 90
SYGNSSDSRY DDGRGPPGNP PRRMGRINHL RGPSPPPMAG GUGR
Length:94
Mass (Da):10,645
Last modified:February 26, 2008 - v3
Checksum:iD26F13E90BEC6807
GO

Sequence cautioni

The sequence AAD40194.1 differs from that shown. Reason: Erroneous termination at position 92. Translated as Sec.Curated
The sequence AAF28975.1 differs from that shown. Reason: Erroneous termination at position 92. Translated as Sec.Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti50 – 501R → S.
Corresponds to variant rs11562 [ dbSNP | Ensembl ].
VAR_053921

Non-standard residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-standard residuei92 – 921Selenocysteine

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF537132 mRNA. Translation: AAN61473.1.
AF085359 mRNA. Translation: AAD40194.1. Sequence problems.
AF161415 mRNA. Translation: AAF28975.1. Sequence problems.
BC013162 mRNA. Translation: AAH13162.2.
CCDSiCCDS54597.1.
RefSeqiNP_067060.2. NM_021237.3.
UniGeneiHs.58471.
Hs.640242.

Genome annotation databases

GeneIDi58515.
KEGGihsa:58515.

Keywords - Coding sequence diversityi

Polymorphism, Selenocysteine

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF537132 mRNA. Translation: AAN61473.1.
AF085359 mRNA. Translation: AAD40194.1. Sequence problems.
AF161415 mRNA. Translation: AAF28975.1. Sequence problems.
BC013162 mRNA. Translation: AAH13162.2.
CCDSiCCDS54597.1.
RefSeqiNP_067060.2. NM_021237.3.
UniGeneiHs.58471.
Hs.640242.

3D structure databases

ProteinModelPortaliQ9Y6D0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121841. 3 interactions.
IntActiQ9Y6D0. 1 interaction.
STRINGi9606.ENSP00000418813.

PTM databases

iPTMnetiQ9Y6D0.
PhosphoSiteiQ9Y6D0.

Polymorphism and mutation databases

BioMutaiSELK.
DMDMi190352222.

Proteomic databases

EPDiQ9Y6D0.
MaxQBiQ9Y6D0.
PaxDbiQ9Y6D0.
PRIDEiQ9Y6D0.

Protocols and materials databases

DNASUi58515.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi58515.
KEGGihsa:58515.

Organism-specific databases

CTDi58515.
GeneCardsiSELK.
H-InvDBHIX0003380.
HPAiHPA008196.
MIMi607916. gene.
neXtProtiNX_Q9Y6D0.

Phylogenomic databases

eggNOGiENOG410J0AZ. Eukaryota.
ENOG410XWKI. LUCA.
GeneTreeiENSGT00390000016119.
HOGENOMiHOG000013002.
HOVERGENiHBG108467.
InParanoidiQ9Y6D0.
OMAiMGRINHW.
PhylomeDBiQ9Y6D0.

Miscellaneous databases

GenomeRNAii58515.
PROiQ9Y6D0.
SOURCEiSearch...

Family and domain databases

InterProiIPR024491. Se_SelK/SelG.
[Graphical view]
PfamiPF10961. DUF2763. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
  2. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
    Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
    , Gu J., Chen S.-J., Chen Z.
    Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Umbilical cord blood.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Adrenal cortex.
  4. Bienvenut W.V., Waridel P., Quadroni M.
    Submitted (MAR-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-13 AND 60-72, CLEAVAGE OF INITIATOR METHIONINE, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryonic kidney.
  5. "Identification and characterization of selenoprotein K: an antioxidant in cardiomyocytes."
    Lu C., Qiu F., Zhou H., Peng Y., Hao W., Xu J., Yuan J., Wang S., Qiang B., Xu C., Peng X.
    FEBS Lett. 580:5189-5197(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. "SelK is a novel ER stress-regulated protein and protects HepG2 cells from ER stress agent-induced apoptosis."
    Du S., Zhou J., Jia Y., Huang K.
    Arch. Biochem. Biophys. 502:137-143(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  7. "Selenoprotein K binds multiprotein complexes and is involved in the regulation of endoplasmic reticulum homeostasis."
    Shchedrina V.A., Everley R.A., Zhang Y., Gygi S.P., Hatfield D.L., Gladyshev V.N.
    J. Biol. Chem. 286:42937-42948(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DERL1; DERL2; DERL3; VCP AND VIMP, IDENTIFICATION IN A COMPLEX WITH CANX; DERL1; DERL2; DDOST; RPN1; RPN2; STT3A; VCP AND VIMP, INDUCTION.
  8. "Selenoprotein K knockout mice exhibit deficient calcium flux in immune cells and impaired immune responses."
    Verma S., Hoffmann F.W., Kumar M., Huang Z., Roe K., Nguyen-Wu E., Hashimoto A.S., Hoffmann P.R.
    J. Immunol. 186:2127-2137(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSELK_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6D0
Secondary accession number(s): Q8IZQ3, Q9P085
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 26, 2008
Last modified: June 8, 2016
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.