Q9Y6B2 (EID1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
EP300-interacting inhibitor of differentiation 1

Alternative name(s):
21 kDa pRb-associated protein
CREBBP/EP300 inhibitory protein 1
E1A-like inhibitor of differentiation 1
Short name=EID-1

Gene names

Name:	EID1
Synonyms:	C15orf3, CRI1, RBP21
ORF Names:	PNAS-22, PTD014

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms. Ref.1 Ref.9 UniProtKB Q9DCR4
Subunit structure	Interacts via its LXCXE motif with the entire pocket region of RB1. Interacts with EP300, NR0B2 and TRIM27. Ref.1 Ref.2 Ref.9 Ref.10 UniProtKB Q9DCR4
Subcellular location	Nucleus. Cytoplasm. Note: May shuttle between nucleus and cytoplasm. Ref.2 UniProtKB Q9DCR4
Tissue specificity	Widely expressed. Most abundantly expressed in heart, skeletal muscle, pancreas, brain and testis. Expressed at much lower levels in placenta and peripheral blood leukocyte. Barely detectable in lung. Also weakly expressed in lung carcinoma A-549 and various leukemia cell lines. Ref.1 Ref.2
Developmental stage	Expression decreased with development in ventricular tissue while remaining highly expressed in adult atrial tissue. In primary cultures of human skeletal myocytes, expression decreased during myogenic differentiation (at protein level). Ref.1
Induction	Down-regulated in differentiating U-937 leukemia cells. Ref.9
Post-translational modification	Ubiquitinated in U2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit. Ref.9
Miscellaneous	Inhibition of MYOD1 may be partly due to the ability of EID1 to bind and inhibit EP300 histone acetyltransferase activity. Ref.1
Sequence caution	The sequence AAK07524.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle Differentiation Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Repressor
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from direct assay Ref.9. Source: UniProtKB negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.1. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from physical interaction Ref.1. Source: MGI
Molecular_function	histone acetyltransferase binding Inferred from direct assay Ref.1. Source: UniProtKB histone acetyltransferase regulator activity Inferred from direct assay Ref.1. Source: UniProtKB transcription corepressor activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Ndn	P25233	5	EBI-1049975,EBI-1801080	From a different organism.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 Ref.2 Ref.3 Ref.4 Ref.7 (identifier: Q9Y6B2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.5 (identifier: Q9Y6B2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     65-87: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 187	187	EP300-interacting inhibitor of differentiation 1		PRO_0000289156
Regions
	Region	54 – 120	67	Interaction with NR0B2 By similarity UniProtKB Q9DCR4
	Motif	178 – 182	5	LXCXE motif Ref.1
Natural variations
	Alternative sequence	65 – 87	23	Missing in isoform 2. Ref.5		VSP_052454
Experimental info
	Mutagenesis	178	1	L → S: Abolishes RB1 binding. Ref.2
	Mutagenesis	180	1	C → G: Abolishes RB1 binding. Ref.1 Ref.2
	Mutagenesis	182	1	E → Q: Abolishes RB1 binding. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: A2815FA78ED0736D	FASTA	187	20,876
	10 20 30 40 50 60 MSEMAELSEL YEESSDLQMD VMPGEGDLPQ MEVGSGSREL SLRPSRSGAQ QLEEEGPMEE 70 80 90 100 110 120 EEAQPMAAPE GKRSLANGPN AGEQPGQVAG ADFESEDEGE EFDDWEDDYD YPEEEQLSGA 130 140 150 160 170 180 GYRVSAALEE ADKMFLRTRE PALDGGFQMH YEKTPFDQLA FIEELFSLMV VNRLTEELGC DEIIDRE
	Isoform 2 [UniParc]. Checksum: FB44C04D87381C53	FASTA	164	18,616
	10 20 30 40 50 60 MSEMAELSEL YEESSDLQMD VMPGEGDLPQ MEVGSGSREL SLRPSRSGAQ QLEEEGPMEE 70 80 90 100 110 120 EEAQVAGADF ESEDEGEEFD DWEDDYDYPE EEQLSGAGYR VSAALEEADK MFLRTREPAL 130 140 150 160 DGGFQMHYEK TPFDQLAFIE ELFSLMVVNR LTEELGCDEI IDRE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel Rb- and p300-binding protein inhibits transactivation by MyoD." MacLellan W.R., Xiao G., Abdellatif M., Schneider M.D. Mol. Cell. Biol. 20:8903-8915(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH EP300 AND RB1, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF CYS-180.
[2]	"Identification and characterization of a novel human cDNA encoding a 21 kDa pRb-associated protein." Wen H., Ao S. Gene 263:85-92(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH RB1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF LEU-178; CYS-180 AND GLU-182.
[3]	"Human PTD014 mRNA, complete cds." Huang Q., Peng Y., Dai M., Song H., Mao Y., Zhang Q., Mao M., Fu G., Luo M., Chen J., Hu R. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Pituitary tumor.
[4]	The European IMAGE consortium Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Neonatal brain and Retina.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain.
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[8]	"Human acute promyelocytic leukemia cell line NB4's apoptosis related genes." Yu W.-Q., Sun B.-Z., Chai Y.-B., Zhu F., Liu X.-S., Li Z., Lu F., Yan W., Yang H., Zhao Z.-L. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 97-187. Tissue: Promyelocytic leukemia.
[9]	"Cells degrade a novel inhibitor of differentiation with E1A-like properties upon exiting the cell cycle." Miyake S., Sellers W.R., Safran M., Li X., Zhao W., Grossman S.R., Gan J., DeCaprio J.A., Adams P.D., Kaelin W.G. Jr. Mol. Cell. Biol. 20:8889-8902(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH EP300 AND RB1, UBIQUITINATION, INDUCTION.
[10]	"Selective ablation of retinoblastoma protein function by the RET finger protein." Krutzfeldt M., Ellis M., Weekes D.B., Bull J.J., Eilers M., Vivanco M.D., Sellers W.R., Mittnacht S. Mol. Cell 18:213-224(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TRIM27.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF349444 mRNA. Translation: AAK29640.1. AF109873 mRNA. Translation: AAG35179.1. AF092135 mRNA. Translation: AAD40377.1. AL109701 mRNA. Translation: CAB52022.1. AL357456 mRNA. Translation: CAB93108.1. AK098383 mRNA. Translation: BAC05296.1. AK315365 mRNA. Translation: BAG37758.1. CH471082 Genomic DNA. Translation: EAW77357.1. BC114944 mRNA. Translation: AAI14945.1. BC114946 mRNA. Translation: AAI14947.1. AF274947 mRNA. Translation: AAK07524.1. Different initiation.
IPI	IPI00295287. IPI00847728.
RefSeq	NP_055150.1. NM_014335.2.
UniGene	Hs.255973.
3D structure databases
ProteinModelPortal	Q9Y6B2.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y6B2. 6 interactions.
PTM databases
PhosphoSite	Q9Y6B2.
Polymorphism databases
DMDM	74721525.
Proteomic databases
PRIDE	Q9Y6B2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000530028; ENSP00000431162; ENSG00000255302.
GeneID	23741.
KEGG	hsa:23741.
UCSC	uc001zxc.1. human.
Organism-specific databases
CTD	23741.
GeneCards	GC15P049171.
H-InvDB	HIX0012222.
HGNC	HGNC:1191. EID1.
HPA	HPA051122. HPA051123.
MIM	605894. gene.
neXtProt	NX_Q9Y6B2.
PharmGKB	PA26876.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	HBG098825.
InParanoid	Q9Y6B2.
OMA	NDLQMDV.
OrthoDB	EOG4X97JM.
Gene expression databases
ArrayExpress	Q9Y6B2.
Bgee	Q9Y6B2.
CleanEx	HS_EID1.
Genevestigator	Q9Y6B2.
Family and domain databases
ProtoNet	Search...
Other
GenomeRNAi	23741.
NextBio	46655.
SOURCE	Search...

Entry information

Entry name

EID1_HUMAN

Accession

Primary (citable) accession number: Q9Y6B2
Secondary accession number(s): B2RD11, Q8N7I4, Q9BZT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.