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Protein

EP300-interacting inhibitor of differentiation 1

Gene

EID1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms.2 Publications

GO - Molecular functioni

  • histone acetyltransferase binding Source: UniProtKB
  • histone acetyltransferase regulator activity Source: UniProtKB
  • transcription corepressor activity Source: UniProtKB

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell differentiation Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of histone acetylation Source: GOC
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Cell cycle, Differentiation, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
EP300-interacting inhibitor of differentiation 1
Alternative name(s):
21 kDa pRb-associated protein
CREBBP/EP300 inhibitory protein 1
E1A-like inhibitor of differentiation 1
Short name:
EID-1
Gene namesi
Name:EID1Imported
Synonyms:C15orf3, CRI1Imported, RBP21
ORF Names:PNAS-22, PTD014
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:1191. EID1.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

  • Note: May shuttle between nucleus and cytoplasm.By similarity1 Publication

GO - Cellular componenti

  • aggresome Source: HPA
  • cytoplasm Source: UniProtKB-SubCell
  • nucleoplasm Source: HPA
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi178 – 1781L → S: Abolishes RB1 binding. 1 Publication
Mutagenesisi180 – 1801C → G: Abolishes RB1 binding. 2 Publications
Mutagenesisi182 – 1821E → Q: Abolishes RB1 binding. 1 Publication

Organism-specific databases

PharmGKBiPA26876.

Polymorphism and mutation databases

BioMutaiEID1.
DMDMi74721525.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 187187EP300-interacting inhibitor of differentiation 1PRO_0000289156Add
BLAST

Post-translational modificationi

Ubiquitinated in U2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

EPDiQ9Y6B2.
MaxQBiQ9Y6B2.
PaxDbiQ9Y6B2.
PRIDEiQ9Y6B2.

PTM databases

iPTMnetiQ9Y6B2.
PhosphoSiteiQ9Y6B2.

Expressioni

Tissue specificityi

Widely expressed. Most abundantly expressed in heart, skeletal muscle, pancreas, brain and testis. Expressed at much lower levels in placenta and peripheral blood leukocyte. Barely detectable in lung. Also weakly expressed in lung carcinoma A-549 and various leukemia cell lines.2 Publications

Developmental stagei

Expression decreased with development in ventricular tissue while remaining highly expressed in adult atrial tissue. In primary cultures of human skeletal myocytes, expression decreased during myogenic differentiation (at protein level).1 Publication

Inductioni

Down-regulated in differentiating U-937 leukemia cells.1 Publication

Gene expression databases

BgeeiQ9Y6B2.
CleanExiHS_EID1.
ExpressionAtlasiQ9Y6B2. baseline and differential.
GenevisibleiQ9Y6B2. HS.

Organism-specific databases

HPAiHPA051122.
HPA051123.

Interactioni

Subunit structurei

Interacts via its LXCXE motif with the entire pocket region of RB1. Interacts with EP300, NR0B2 and TRIM27.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NdnP252335EBI-1049975,EBI-1801080From a different organism.

GO - Molecular functioni

  • histone acetyltransferase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi117243. 32 interactions.
IntActiQ9Y6B2. 10 interactions.
MINTiMINT-149992.
STRINGi9606.ENSP00000431162.

Structurei

3D structure databases

ProteinModelPortaliQ9Y6B2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni54 – 12067Interaction with NR0B2By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi178 – 1825LXCXE motif1 Publication

Phylogenomic databases

eggNOGiENOG410J09N. Eukaryota.
ENOG41118MI. LUCA.
GeneTreeiENSGT00500000045230.
HOVERGENiHBG098825.
InParanoidiQ9Y6B2.
OMAiMAEPQGE.
OrthoDBiEOG7SXW53.
PhylomeDBiQ9Y6B2.
TreeFamiTF337633.

Family and domain databases

InterProiIPR033258. EID.
IPR033255. EID-1.
[Graphical view]
PANTHERiPTHR15556. PTHR15556. 1 hit.
PTHR15556:SF5. PTHR15556:SF5. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 14 Publications (identifier: Q9Y6B2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEMAELSEL YEESSDLQMD VMPGEGDLPQ MEVGSGSREL SLRPSRSGAQ
60 70 80 90 100
QLEEEGPMEE EEAQPMAAPE GKRSLANGPN AGEQPGQVAG ADFESEDEGE
110 120 130 140 150
EFDDWEDDYD YPEEEQLSGA GYRVSAALEE ADKMFLRTRE PALDGGFQMH
160 170 180
YEKTPFDQLA FIEELFSLMV VNRLTEELGC DEIIDRE
Length:187
Mass (Da):20,876
Last modified:November 1, 1999 - v1
Checksum:iA2815FA78ED0736D
GO
Isoform 21 Publication (identifier: Q9Y6B2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     65-87: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:164
Mass (Da):18,616
Checksum:iFB44C04D87381C53
GO

Sequence cautioni

The sequence AAK07524.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei65 – 8723Missing in isoform 2. 1 PublicationVSP_052454Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF349444 mRNA. Translation: AAK29640.1.
AF109873 mRNA. Translation: AAG35179.1.
AF092135 mRNA. Translation: AAD40377.1.
AL109701 mRNA. Translation: CAB52022.1.
AL357456 mRNA. Translation: CAB93108.1.
AK098383 mRNA. Translation: BAC05296.1.
AK315365 mRNA. Translation: BAG37758.1.
CH471082 Genomic DNA. Translation: EAW77357.1.
BC114944 mRNA. Translation: AAI14945.1.
BC114946 mRNA. Translation: AAI14947.1.
AF274947 mRNA. Translation: AAK07524.1. Different initiation.
CCDSiCCDS53941.1. [Q9Y6B2-1]
RefSeqiNP_055150.1. NM_014335.2. [Q9Y6B2-1]
UniGeneiHs.255973.

Genome annotation databases

EnsembliENST00000530028; ENSP00000431162; ENSG00000255302. [Q9Y6B2-1]
GeneIDi23741.
KEGGihsa:23741.
UCSCiuc001zxc.2. human. [Q9Y6B2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF349444 mRNA. Translation: AAK29640.1.
AF109873 mRNA. Translation: AAG35179.1.
AF092135 mRNA. Translation: AAD40377.1.
AL109701 mRNA. Translation: CAB52022.1.
AL357456 mRNA. Translation: CAB93108.1.
AK098383 mRNA. Translation: BAC05296.1.
AK315365 mRNA. Translation: BAG37758.1.
CH471082 Genomic DNA. Translation: EAW77357.1.
BC114944 mRNA. Translation: AAI14945.1.
BC114946 mRNA. Translation: AAI14947.1.
AF274947 mRNA. Translation: AAK07524.1. Different initiation.
CCDSiCCDS53941.1. [Q9Y6B2-1]
RefSeqiNP_055150.1. NM_014335.2. [Q9Y6B2-1]
UniGeneiHs.255973.

3D structure databases

ProteinModelPortaliQ9Y6B2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117243. 32 interactions.
IntActiQ9Y6B2. 10 interactions.
MINTiMINT-149992.
STRINGi9606.ENSP00000431162.

PTM databases

iPTMnetiQ9Y6B2.
PhosphoSiteiQ9Y6B2.

Polymorphism and mutation databases

BioMutaiEID1.
DMDMi74721525.

Proteomic databases

EPDiQ9Y6B2.
MaxQBiQ9Y6B2.
PaxDbiQ9Y6B2.
PRIDEiQ9Y6B2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000530028; ENSP00000431162; ENSG00000255302. [Q9Y6B2-1]
GeneIDi23741.
KEGGihsa:23741.
UCSCiuc001zxc.2. human. [Q9Y6B2-1]

Organism-specific databases

CTDi23741.
GeneCardsiEID1.
H-InvDBHIX0012222.
HGNCiHGNC:1191. EID1.
HPAiHPA051122.
HPA051123.
MIMi605894. gene.
neXtProtiNX_Q9Y6B2.
PharmGKBiPA26876.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410J09N. Eukaryota.
ENOG41118MI. LUCA.
GeneTreeiENSGT00500000045230.
HOVERGENiHBG098825.
InParanoidiQ9Y6B2.
OMAiMAEPQGE.
OrthoDBiEOG7SXW53.
PhylomeDBiQ9Y6B2.
TreeFamiTF337633.

Miscellaneous databases

GeneWikiiEID1.
GenomeRNAii23741.
NextBioi46655.
PROiQ9Y6B2.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Y6B2.
CleanExiHS_EID1.
ExpressionAtlasiQ9Y6B2. baseline and differential.
GenevisibleiQ9Y6B2. HS.

Family and domain databases

InterProiIPR033258. EID.
IPR033255. EID-1.
[Graphical view]
PANTHERiPTHR15556. PTHR15556. 1 hit.
PTHR15556:SF5. PTHR15556:SF5. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel Rb- and p300-binding protein inhibits transactivation by MyoD."
    MacLellan W.R., Xiao G., Abdellatif M., Schneider M.D.
    Mol. Cell. Biol. 20:8903-8915(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH EP300 AND RB1, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF CYS-180.
  2. "Identification and characterization of a novel human cDNA encoding a 21 kDa pRb-associated protein."
    Wen H., Ao S.
    Gene 263:85-92(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH RB1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF LEU-178; CYS-180 AND GLU-182.
  3. "Human PTD014 mRNA, complete cds."
    Huang Q., Peng Y., Dai M., Song H., Mao Y., Zhang Q., Mao M., Fu G., Luo M., Chen J., Hu R.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Pituitary tumorImported.
  4. The European IMAGE consortium
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Neonatal brainImported and RetinaImported.
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: BrainImported.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  8. "Human acute promyelocytic leukemia cell line NB4's apoptosis related genes."
    Yu W.-Q., Sun B.-Z., Chai Y.-B., Zhu F., Liu X.-S., Li Z., Lu F., Yan W., Yang H., Zhao Z.-L.
    Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 97-187.
    Tissue: Promyelocytic leukemiaImported.
  9. "Cells degrade a novel inhibitor of differentiation with E1A-like properties upon exiting the cell cycle."
    Miyake S., Sellers W.R., Safran M., Li X., Zhao W., Grossman S.R., Gan J., DeCaprio J.A., Adams P.D., Kaelin W.G. Jr.
    Mol. Cell. Biol. 20:8889-8902(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH EP300 AND RB1, UBIQUITINATION, INDUCTION.
  10. "Selective ablation of retinoblastoma protein function by the RET finger protein."
    Krutzfeldt M., Ellis M., Weekes D.B., Bull J.J., Eilers M., Vivanco M.D., Sellers W.R., Mittnacht S.
    Mol. Cell 18:213-224(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TRIM27.

Entry informationi

Entry nameiEID1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6B2
Secondary accession number(s): B2RD11, Q8N7I4, Q9BZT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: November 1, 1999
Last modified: May 11, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Inhibition of MYOD1 may be partly due to the ability of EID1 to bind and inhibit EP300 histone acetyltransferase activity.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.