Q9Y6A9 (SPCS1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal peptidase complex subunit 1
EC=3.4.-.-

Alternative name(s):
Microsomal signal peptidase 12 kDa subunit
Short name=SPase 12 kDa subunit

Gene names

Name:	SPCS1
Synonyms:	SPC12
ORF Names:	HSPC033

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum By similarity.
Subunit structure	Component of the microsomal signal peptidase complex which consists of five members: SEC11A, SEC11C, SPCS1, SPCS2 and SPCS3 By similarity.
Subcellular location	Microsome membrane; Multi-pass membrane protein By similarity. Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the SPCS1 family.
Caution	It is uncertain whether Met-1 or Met-68 is the initiator.
Sequence caution	The sequence AAD40380.1 differs from that shown. Reason: Frameshift at positions 31 and 70. The sequence AAH00884.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAL31361.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAG51320.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	energy reserve metabolic process Traceable author statement. Source: Reactome proteolysis Traceable author statement Ref.4. Source: UniProtKB regulation of insulin secretion Traceable author statement. Source: Reactome signal peptide processing Inferred from electronic annotation. Source: InterPro
Cellular component	integral to endoplasmic reticulum membrane Inferred from direct assay Ref.4. Source: UniProtKB microsome Inferred from electronic annotation. Source: UniProtKB-SubCell signal peptidase complex Traceable author statement Ref.4. Source: UniProtKB
Molecular function	peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 169	169	Signal peptidase complex subunit 1		PRO_0000215154
Regions
	Topological domain	1 – 93	93	Cytoplasmic Potential
	Transmembrane	94 – 114	21	Helical; Potential
	Topological domain	115	1	Extracellular Potential
	Transmembrane	116 – 136	21	Helical; Potential
	Topological domain	137 – 169	33	Cytoplasmic Potential
Experimental info
	Sequence conflict	41	1	P → A in AAH00884. Ref.5
	Sequence conflict	41	1	P → A in AAD40380. Ref.2
	Sequence conflict	95	1	I → Y in AAL31361. Ref.4
	Sequence conflict	128	1	L → LAQ in AAH00884. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9Y6A9 [UniParc]. Last modified March 8, 2011. Version 3. Checksum: 65F180938A9BEF1E	FASTA	169	18,298
	10 20 30 40 50 60 MARGGDTGCT GPSETSASGA AAIALPGLEG PATDAQCQTL PLTVLKSRSP SPRSLPPALS 70 80 90 100 110 120 CPPPQPAMLE HLSSLPTQMD YKGQKLAEQM FQGIILFSAI VGFIYGYVAE QFGWTVYIVM 130 140 150 160 AGFAFSCLLT LPPWPIYRRH PLKWLPVQES STDDKKPGER KIKRHAKNN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed: 16641997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. , Gu J., Chen S.-J., Chen Z. Genome Res. 10:1546-1560(2000) [PubMed: 11042152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 23-169. Tissue: Umbilical cord blood.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 23-169.
[4]	"Membrane topology of the 12- and the 25-kDa subunits of the mammalian signal peptidase complex." Kalies K.-U., Hartmann E. J. Biol. Chem. 271:3925-3929(1996) [PubMed: 8632014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 27-169, TOPOLOGY.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 32-169. Tissue: Cervix.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC104446 Genomic DNA. No translation available. AF092138 mRNA. Translation: AAD40380.1. Frameshift. AK027426 mRNA. Translation: BAG51320.1. Different initiation. L38852 mRNA. Translation: AAL31361.1. Different initiation. BC000884 mRNA. Translation: AAH00884.1. Different initiation.
IPI	IPI00924556.
RefSeq	NP_054760.3. NM_014041.3.
UniGene	Hs.11125.
3D structure databases
ProteinModelPortal	Q9Y6A9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9Y6A9.
Polymorphism databases
DMDM	20140350.
Proteomic databases
PRIDE	Q9Y6A9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000233025; ENSP00000233025; ENSG00000114902. ENST00000426887; ENSP00000398800; ENSG00000114902.
GeneID	28972.
KEGG	hsa:28972.
UCSC	uc003dfp.2. human.
Organism-specific databases
CTD	28972.
GeneCards	GC03P052738.
H-InvDB	HIX0003369.
HGNC	HGNC:23401. SPCS1.
HPA	HPA046091.
MIM	610358. gene.
neXtProt	NX_Q9Y6A9.
PharmGKB	PA134972486.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG20546.
GeneTree	ENSGT00390000018321.
HOVERGEN	HBG086276.
InParanoid	Q9Y6A9.
OMA	QPAMLEH.
OrthoDB	EOG418BPZ.
PhylomeDB	Q9Y6A9.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism. REACT_15380. Diabetes pathways.
Gene expression databases
ArrayExpress	Q9Y6A9.
Bgee	Q9Y6A9.
CleanEx	HS_SPCS1.
Genevestigator	Q9Y6A9.
GermOnline	ENSG00000114902. Homo sapiens.
Family and domain databases
InterPro	IPR009542. SPC12. [Graphical view]
KO	K12946.
PANTHER	PTHR13202. SPC12. 1 hit.
Pfam	PF06645. SPC12. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

SPCS1_HUMAN

Accession

Primary (citable) accession number: Q9Y6A9
Secondary accession number(s): B3KNF8, Q9BVW1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	March 8, 2011
Last modified:	January 25, 2012
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.