Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9Y694 (S22A7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Solute carrier family 22 member 7
Alternative name(s):
Novel liver transporter
Organic anion transporter 2
Short name=hOAT2
Gene names
Name:SLC22A7
Synonyms:NLT, OAT2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length548 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates sodium-independent multispecific organic anion transport. Transport of prostaglandin E2, prostaglandin F2, tetracycline, bumetanide, estrone sulfate, glutarate, dehydroepiandrosterone sulfate, allopurinol, 5-fluorouracil, paclitaxel, L-ascorbic acid, salicylate, ethotrexate, and alpha-ketoglutarate. Ref.9 Ref.10 Ref.11 Ref.12 Ref.13

Subcellular location

Basolateral cell membrane; Multi-pass membrane protein. Note: Apical side of the renal tubule. Ref.10 Ref.11

Tissue specificity

Expressed in liver and kidney. Ref.9 Ref.11 Ref.13

Sequence similarities

Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. [View classification]

Biophysicochemical properties

Kinetic parameters:

KM=713 nM for prostaglandin E2 Ref.11 Ref.12

KM=440 µM for tetracycline

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Y694-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Y694-2)

The sequence of this isoform differs from the canonical sequence as follows:
     132-133: Missing.
Isoform 3 (identifier: Q9Y694-3)

The sequence of this isoform differs from the canonical sequence as follows:
     132-133: Missing.
     531-548: SAPTSLQEEEMPMKQVQN → RCVHRTVSVYV
Isoform 4 (identifier: Q9Y694-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-307: Missing.
     308-317: PVCEDSFSQE → MLQPHLLPYQ

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 548548Solute carrier family 22 member 7
PRO_0000317481

Regions

Transmembrane21 – 4121Helical; Potential
Transmembrane146 – 16621Helical; Potential
Transmembrane180 – 20021Helical; Potential
Transmembrane204 – 22421Helical; Potential
Transmembrane234 – 25421Helical; Potential
Transmembrane259 – 27921Helical; Potential
Transmembrane346 – 36621Helical; Potential
Transmembrane376 – 39722Helical; Potential
Transmembrane404 – 42320Helical; Potential
Transmembrane432 – 45221Helical; Potential
Transmembrane466 – 48621Helical; Potential
Transmembrane493 – 51321Helical; Potential

Amino acid modifications

Glycosylation911N-linked (GlcNAc...) Ref.14

Natural variations

Alternative sequence1 – 307307Missing in isoform 4.
VSP_030991
Alternative sequence132 – 1332Missing in isoform 2 and isoform 3.
VSP_030992
Alternative sequence308 – 31710PVCEDSFSQE → MLQPHLLPYQ in isoform 4.
VSP_030993
Alternative sequence531 – 54818SAPTS…KQVQN → RCVHRTVSVYV in isoform 3.
VSP_030994
Natural variant3031R → K. Ref.5
Corresponds to variant rs70953684 [ dbSNP | Ensembl ].
VAR_047875
Natural variant3271R → W. Ref.5
Corresponds to variant rs36040909 [ dbSNP | Ensembl ].
VAR_047876
Natural variant5071G → V. Ref.5
Corresponds to variant rs70953693 [ dbSNP | Ensembl ].
VAR_047877

Experimental info

Sequence conflict3041L → P in AAG43523. Ref.2
Sequence conflict4131T → A in AAG43523. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 5BA4C3FCC3C57533

FASTA54860,026
        10         20         30         40         50         60 
MGFEELLEQV GGFGPFQLRN VALLALPRVL LPLHFLLPIF LAAVPAHRCA LPGAPANFSH 

        70         80         90        100        110        120 
QDVWLEAHLP REPDGTLSSC LRFAYPQALP NTTLGEERQS RGELEDEPAT VPCSQGWEYD 

       130        140        150        160        170        180 
HSEFSSTIAT ESQWDLVCEQ KGLNRAASTF FFAGVLVGAV AFGYLSDRFG RRRLLLVAYV 

       190        200        210        220        230        240 
STLVLGLASA ASVSYVMFAI TRTLTGSALA GFTIIVMPLE LEWLDVEHRT VAGVLSSTFW 

       250        260        270        280        290        300 
TGGVMLLALV GYLIRDWRWL LLAVTLPCAP GILSLWWVPE SARWLLTQGH VKEAHRYLLH 

       310        320        330        340        350        360 
CARLNGRPVC EDSFSQEAVS KVAAGERVVR RPSYLDLFRT PRLRHISLCC VVVWFGVNFS 

       370        380        390        400        410        420 
YYGLSLDVSG LGLNVYQTQL LFGAVELPSK LLVYLSVRYA GRRLTQAGTL LGTALAFGTR 

       430        440        450        460        470        480 
LLVSSDMKSW STVLAVMGKA FSEAAFTTAY LFTSELYPTV LRQTGMGLTA LVGRLGGSLA 

       490        500        510        520        530        540 
PLAALLDGVW LSLPKLTYGG IALLAAGTAL LLPETRQAQL PETIQDVERK SAPTSLQEEE 


MPMKQVQN 

« Hide

Isoform 2 [UniParc].

Checksum: 6A87AA1BD6FEA15C
Show »

FASTA54659,810
Isoform 3 [UniParc].

Checksum: F3D6F799778EFE63
Show »

FASTA53959,082
Isoform 4 [UniParc].

Checksum: 8AF6769F5CAC8439
Show »

FASTA24126,213

References

« Hide 'large scale' references
[1]"Molecular cloning of a human liver-specific transporter, NLT."
Kim R.B., Leake B.L., Cvetkovic M.
Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Liver.
[2]"Identification of a human organic anion transporter 2 homolog."
Reid G., Bahn A., Ebbinghaus C., Wolff N.A., Burckhardt G.
Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
Tissue: Kidney.
[3]"Cloning, characterization and localization of human organic anion transporter 2."
Kobayashi Y., Ohshiro N., Sekine T., Endoh H., Yamamoto T.
Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Tissue: Kidney.
[5]NIEHS SNPs program
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS LYS-303; TRP-327 AND VAL-507.
[6]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Colon.
[9]"Isolation of a family of organic anion transporters from human liver and kidney."
Sun W., Wu R.R., van Poelje P.D., Erion M.D.
Biochem. Biophys. Res. Commun. 283:417-422(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[10]"Interaction of human organic anion transporters 2 and 4 with organic anion transport inhibitors."
Enomoto A., Takeda M., Shimoda M., Narikawa S., Kobayashi Y., Kobayashi Y., Yamamoto T., Sekine T., Cha S.H., Niwa T., Endou H.
J. Pharmacol. Exp. Ther. 301:797-802(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[11]"Human organic anion transporters and human organic cation transporters mediate renal transport of prostaglandins."
Kimura H., Takeda M., Narikawa S., Enomoto A., Ichida K., Endou H.
J. Pharmacol. Exp. Ther. 301:293-298(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
[12]"Human organic anion transporters mediate the transport of tetracycline."
Babu E., Takeda M., Narikawa S., Kobayashi Y., Yamamoto T., Cha S.H., Sekine T., Sakthisekaran D., Endou H.
Jpn. J. Pharmacol. 88:69-76(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[13]"Transport mechanism and substrate specificity of human organic anion transporter 2 (hOat2 [SLC22A7])."
Kobayashi Y., Ohshiro N., Sakai R., Ohbayashi M., Kohyama N., Yamamoto T.
J. Pharm. Pharmacol. 57:573-578(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[14]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-91.
Tissue: Liver.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF097518 mRNA. Translation: AAD37091.1.
AF210455 mRNA. Translation: AAG43523.1.
AY050498 mRNA. Translation: AAL12496.1.
AK290796 mRNA. Translation: BAF83485.1.
AK290806 mRNA. Translation: BAF83495.1.
EU562669 Genomic DNA. Translation: ACB21045.1.
AL583834 Genomic DNA. Translation: CAI14466.1.
AL583834 Genomic DNA. Translation: CAI14462.1.
AL583834 Genomic DNA. Translation: CAI14463.1.
AL583834 Genomic DNA. Translation: CAI14464.1.
CH471081 Genomic DNA. Translation: EAX04172.1.
CH471081 Genomic DNA. Translation: EAX04175.1.
BC033805 mRNA. Translation: AAH33805.1.
RefSeqNP_006663.2. NM_006672.3.
NP_696961.2. NM_153320.2.
UniGeneHs.485438.

3D structure databases

ProteinModelPortalQ9Y694.
SMRQ9Y694. Positions 140-517.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000361666.

Chemistry

ChEMBLCHEMBL1955711.

Protein family/group databases

TCDB2.A.1.19.25. the major facilitator superfamily (mfs).

PTM databases

PhosphoSiteQ9Y694.

Polymorphism databases

DMDM74753520.

Proteomic databases

PaxDbQ9Y694.
PRIDEQ9Y694.

Protocols and materials databases

DNASU10864.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000372574; ENSP00000361655; ENSG00000137204. [Q9Y694-3]
ENST00000372585; ENSP00000361666; ENSG00000137204. [Q9Y694-1]
ENST00000372589; ENSP00000361670; ENSG00000137204. [Q9Y694-2]
GeneID10864.
KEGGhsa:10864.
UCSCuc003ous.3. human. [Q9Y694-3]
uc003out.3. human. [Q9Y694-1]

Organism-specific databases

CTD10864.
GeneCardsGC06P043263.
HGNCHGNC:10971. SLC22A7.
HPAHPA030220.
MIM604995. gene.
neXtProtNX_Q9Y694.
PharmGKBPA35848.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0477.
HOGENOMHOG000234569.
HOVERGENHBG108433.
KOK08204.
OMAPMKQVQN.
OrthoDBEOG7C8GH9.
PhylomeDBQ9Y694.
TreeFamTF315847.

Enzyme and pathway databases

ReactomeREACT_15518. Transmembrane transport of small molecules.

Gene expression databases

ArrayExpressQ9Y694.
BgeeQ9Y694.
CleanExHS_SLC22A7.
GenevestigatorQ9Y694.

Family and domain databases

InterProIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004749. Orgcat_transp.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 1 hit.
TIGRFAMsTIGR00898. 2A0119. 1 hit.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiSLC22A7.
GenomeRNAi10864.
NextBio41247.
PROQ9Y694.
SOURCESearch...

Entry information

Entry nameS22A7_HUMAN
AccessionPrimary (citable) accession number: Q9Y694
Secondary accession number(s): B2CZX6 expand/collapse secondary AC list , Q5T046, Q5T048, Q5T050, Q9H2W5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 1, 1999
Last modified: April 16, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM