Q9Y689 (ARL5A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ADP-ribosylation factor-like protein 5A

Gene names

Name:	ARL5A
Synonyms:	ARFLP5, ARL5

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Lacks ADP-ribosylation enhancing activity By similarity.
Sequence similarities	Belongs to the small GTPase superfamily. Arf family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Myristate
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y689-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y689-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-37: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Potential

Chain

2 – 179

178

ADP-ribosylation factor-like protein 5A

PRO_0000207467

Regions

Nucleotide binding

23 – 30

8

GTP

Nucleotide binding

66 – 70

5

GTP By similarity

Nucleotide binding

125 – 128

4

GTP

Sites

Binding site

159

1

GTP; via amide nitrogen

Amino acid modifications

Lipidation

2

1

N-myristoyl glycine Potential

Natural variations

Alternative sequence

1 – 37

37

Missing in isoform 2.

VSP_041017

Secondary structure

1

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179

Helix Strand Turn

Details...

Helix

4 – 12

9

Beta strand

17 – 24

8

Helix

29 – 35

7

Turn

36 – 39

4

Beta strand

42 – 46

5

Beta strand

48 – 51

4

Beta strand

53 – 57

5

Beta strand

60 – 68

9

Helix

69 – 71

3

Beta strand

86 – 92

7

Turn

96 – 98

3

Helix

99 – 110

12

Helix

113 – 115

3

Beta strand

119 – 125

7

Helix

135 – 141

7

Helix

144 – 146

3

Beta strand

152 – 156

5

Turn

159 – 162

4

Helix

165 – 173

9

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 431CC65F8D8CB9D5	FASTA	179	20,728
	10 20 30 40 50 60 MGILFTRIWR LFNHQEHKVI IVGLDNAGKT TILYQFSMNE VVHTSPTIGS NVEEIVINNT 70 80 90 100 110 120 RFLMWDIGGQ ESLRSSWNTY YTNTEFVIVV VDSTDRERIS VTREELYKML AHEDLRKAGL 130 140 150 160 170 LIFANKQDVK ECMTVAEISQ FLKLTSIKDH QWHIQACCAL TGEGLCQGLE WMMSRLKIR
	Isoform 2 [UniParc]. Checksum: 00FEAA9777FEEED7	FASTA	142	16,385
	10 20 30 40 50 60 MNEVVHTSPT IGSNVEEIVI NNTRFLMWDI GGQESLRSSW NTYYTNTEFV IVVVDSTDRE 70 80 90 100 110 120 RISVTREELY KMLAHEDLRK AGLLIFANKQ DVKECMTVAE ISQFLKLTSI KDHQWHIQAC 130 140 CALTGEGLCQ GLEWMMSRLK IR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of nine novel human small GTPases showing variable expressions in liver cancer tissues." He H., Dai F.Y., Yu L., She X., Zhao Y., Jiang J., Chen X., Zhao S.Y. Gene Expr. 10:231-242(2002) [PubMed: 12450215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed: 10931946] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Pituitary.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Thymus.
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[5]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"Full-length cDNA libraries and normalization." Li W.B., Gruber C., Jessee J., Polayes D. Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[7]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta.
[10]	"2.0 A crystal structure of human ARL5-GDP3'P, a novel member of the small GTP-binding proteins." Wang Z.-X., Shi L., Liu J.-F., An X.-M., Chang W.-R., Liang D.-C. Biochem. Biophys. Res. Commun. 332:640-645(2005) [PubMed: 15896705] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH GTP ANALOG.
[11]	"Structure of human ADP-ribosylation factor-like 5 (ARL5)." Structural genomics consortium (SGC) Submitted (JUN-2006) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 15-176.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF087889 mRNA. Translation: AAP97188.1.
AF100740 mRNA. Translation: AAD40383.1.
AK292714 mRNA. Translation: BAF85403.1.
AF493891 mRNA. Translation: AAM12605.1.
BT009829 mRNA. Translation: AAP88831.1.
CR613929 mRNA. No translation available.
AC097448 Genomic DNA. Translation: AAX82013.1.
CH471058 Genomic DNA. Translation: EAX11495.1.
CH471058 Genomic DNA. Translation: EAX11496.1.
BC001254 mRNA. Translation: AAH01254.1.

IPI

IPI00001923.
IPI00412787.

RefSeq

NP_001032251.1. NM_001037174.1.
NP_036229.1. NM_012097.3.
NP_817114.2. NM_177985.2.

UniGene

Hs.470233.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Z6Y	X-ray	2.40	A/B	1-179	[»]
1ZJ6	X-ray	2.00	A	1-179	[»]
2H16	X-ray	2.00	A/B/C/D	14-177	[»]
2H17	X-ray	1.70	A	15-176	[»]

ProteinModelPortal

Q9Y689.

SMR

Q9Y689. Positions 16-175.

ModBase

Search...

Protein-protein interaction databases

STRING

Q9Y689.

Polymorphism databases

DMDM

12229600.

Proteomic databases

PRIDE

Q9Y689.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000295087; ENSP00000295087; ENSG00000162980.

GeneID

26225.

KEGG

hsa:26225.

UCSC

uc002txx.1. human.

Organism-specific databases

CTD

26225.

GeneCards

GC02M152645.

H-InvDB

HIX0200252.

HGNC

HGNC:696. ARL5A.

HPA

HPA027002.

MIM

608960. gene.

neXtProt

NX_Q9Y689.

PharmGKB

PA24989.

GenAtlas

Search...

Phylogenomic databases

GeneTree

ENSGT00600000084214.

HOGENOM

HBG745225.

HOVERGEN

HBG002073.

InParanoid

Q9Y689.

OMA

KQDVKGC.

OrthoDB

EOG4NS3CM.

PhylomeDB

Q9Y689.

Gene expression databases

ArrayExpress

Q9Y689.

Bgee

Q9Y689.

CleanEx

HS_ARL5A.

Genevestigator

Q9Y689.

GermOnline

ENSG00000162980. Homo sapiens.

Family and domain databases

InterPro

IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]

KO

K07949.

PANTHER

PTHR11711. ARF/SAR. 1 hit.

Pfam

PF00025. Arf. 1 hit.
[Graphical view]

PRINTS

PR00328. SAR1GTPBP.

SMART

SM00177. ARF. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. Small_GTP. 1 hit.

PROSITE

PS51417. ARF. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

48373.

SOURCE

Search...

Entry information

Entry name

ARL5A_HUMAN

Accession

Primary (citable) accession number: Q9Y689
Secondary accession number(s): Q580I5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1999
Last modified:	January 25, 2012
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.