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Reviewed, UniProtKB/Swiss-Prot Q9Y679 (AUP1_HUMAN)

Last modified March 2, 2010. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
Ancient ubiquitous protein 1
Gene names
Name:AUP1
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length476 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. Ref.9

Subunit structure

Identified in a complex that contains SEL1L, OS9, FAF2/UBXD8, UBE2J1/UBC6E and AUP1. Interacts with the cytoplasmic tail of ITGA2B, ITGA1, ITGA2, ITGA5, ITGAV and ITGAM. Ref.7

Subcellular location

Endoplasmic reticulum membrane; Single-pass type III membrane protein; Cytoplasmic side Ref.9 Ref.7.

Tissue specificity

Detected in blood platelets and leukocytes (at protein level). Ubiquitous. Highly expressed in placenta, liver, kidney, skeletal muscle, heart and brain. Ref.7

Sequence similarities

Belongs to the AUP1 family.

Contains 1 CUE domain.

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Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentEndoplasmic reticulum
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processmodification-dependent protein catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionprotein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: Q9Y679-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: Q9Y679-2)

The sequence of this isoform differs from the canonical sequence as follows:
     114-179: Missing.
Isoform 3 (identifier: Q9Y679-3)

The sequence of this isoform differs from the canonical sequence as follows:
     114-179: Missing.
     426-439: FPSSGPVTPQPTAL → AFDACLMMMTPQAL
     440-476: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 476476Ancient ubiquitous protein 1
PRO_0000020765

Regions

Topological domain1 – 2020Lumenal Potential
Transmembrane21 – 4121Signal-anchor for type III membrane protein Potential
Topological domain42 – 476435Cytoplasmic Potential
Domain362 – 40443CUE
Compositional bias160 – 1656Poly-Phe

Amino acid modifications

Modified residue3541Phosphoserine Ref.8
Modified residue3581Phosphoserine Ref.8
Modified residue4221Phosphoserine Ref.8
Modified residue4291Phosphoserine Ref.8
Modified residue4331Phosphothreonine Ref.8 Ref.11

Natural variations

Alternative sequence114 – 17966Missing in isoform Short and isoform 3.
VSP_004133
Alternative sequence426 – 43914FPSSG…QPTAL → AFDACLMMMTPQAL in isoform 3.
VSP_036228
Alternative sequence440 – 47637Missing in isoform 3.
VSP_036229

Experimental info

Sequence conflict3541S → P in AAD43010. Ref.3

Secondary structure

1 ......... 476
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: A6AF3AAD84AE86CB

FASTA47653,028
        10         20         30         40         50         60 
MELPSGPGPE RLFDSHRLPG DCFLLLVLLL YAPVGFCLLV LRLFLGIHVF LVSCALPDSV 

        70         80         90        100        110        120 
LRRFVVRTMC AVLGLVARQE DSGLRDHSVR VLISNHVTPF DHNIVNLLTT CSTVSESEAE 

       130        140        150        160        170        180 
SATGRFPGAQ LKAPLSPLAF RMEDTEALPL TPILYPTCQF FFFIFLNIFL LAFSSPGSQP 

       190        200        210        220        230        240 
LLNSPPSFVC WSRGFMEMNG RGELVESLKR FCASTRLPPT PLLLFPEEEA TNGREGLLRF 

       250        260        270        280        290        300 
SSWPFSIQDV VQPLTLQVQR PLVSVTVSDA SWVSELLWSL FVPFTVYQVR WLRPVHRQLG 

       310        320        330        340        350        360 
EANEEFALRV QQLVAKELGQ TGTRLTPADK AEHMKRQRHP RLRPQSAQSS FPPSPGPSPD 

       370        380        390        400        410        420 
VQLATLAQRV KEVLPHVPLG VIQRDLAKTG CVDLTITNLL EGAVAFMPED ITKGTQSLPT 

       430        440        450        460        470 
ASASKFPSSG PVTPQPTALT FAKSSWARQE SLQERKQALY EYARRRFTER RAQEAD

« Hide

Isoform Short.

Checksum: 1A06476225C5DFDC
Show »

FASTA41045,787
Isoform 3.

Checksum: AAB256D01055538E
Show »

FASTA37341,385

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References

« Hide 'large scale' references
[1]"Human ancient ubiquitous protein AUP1 isoform gene."
Peng Y., Song H., Dai M., Huang Q., Mao Y., Zhang Q., Mao M., Fu G., Luo M., Chen J., Hu R.
Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
Tissue: Pituitary.
[2]"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. expand/collapse author list , Gu J., Chen S.-J., Chen Z.
Genome Res. 10:1546-1560(2000) [PubMed: 11042152] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
Tissue: Umbilical cord blood.
[3]"Novel genes expressed in hematopoietic stem/progenitor cells from myelodysplastic syndrome patients."
Gu J., Huang Q., Yu Y., Xu S., Han Z., Fu G., Zhou J., Wang Y., Huang C., Ren S., Tu Y., Chen Z.
Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
Tissue: Hematopoietic stem cell.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
Tissue: Eye.
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND SHORT).
Tissue: Eye and Skin.
[7]"Ancient ubiquitous protein 1 binds to the conserved membrane-proximal sequence of the cytoplasmic tail of the integrin alpha subunits that plays a crucial role in the inside-out signaling of alpha IIbbeta 3."
Kato A., Kawamata N., Tamayose K., Egashira M., Miura R., Fujimura T., Murayama K., Oshimi K.
J. Biol. Chem. 277:28934-28941(2002) [PubMed: 12042322] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ITGA2B; ITGA1; ITGA2; ITGA5; ITGAV AND ITGAM, TISSUE SPECIFICITY.
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354; SER-358; SER-422; SER-429 AND THR-433, MASS SPECTROMETRY.
[9]"SEL1L nucleates a protein complex required for dislocation of misfolded glycoproteins."
Mueller B., Klemm E.J., Spooner E., Claessen J.H., Ploegh H.L.
Proc. Natl. Acad. Sci. U.S.A. 105:12325-12330(2008) [PubMed: 18711132] [Abstract]
Cited for: IDENTIFICATION IN A COMPLEX WITH SEL1L, SUBCELLULAR LOCATION, FUNCTION.
[10]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[11]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-433, MASS SPECTROMETRY.
[12]"Solution structure of RSGI RUH-075, a human CUE domain."
RIKEN structural genomics initiative (RSGI)
Submitted (SEP-2007) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 358-411.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF100754 mRNA. Translation: AAD43018.1.
AF100753 mRNA. Translation: AAD43017.1.
AF100746 mRNA. Translation: AAD43010.1.
AF165515 mRNA. Translation: AAF86645.1.
AK023983 mRNA. Translation: BAB14753.1.
CH471053 Genomic DNA. Translation: EAW99623.1.
BC001658 mRNA. Translation: AAH01658.1.
BC033646 mRNA. Translation: AAH33646.2. Different initiation.
IPIIPI00001891.
IPI00215815.
IPI00917463.
RefSeqNP_853553.1.
UniGeneHs.411480

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2EKFNMR-A358-411[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-46260N.
IntActQ9Y679. 8 interactions.
STRINGQ9Y679.

PTM databases

PhosphoSiteQ9Y679.

Proteomic databases

PRIDEQ9Y679.

Genome annotation databases

EnsemblENST00000258081; ENSP00000258081; ENSG00000115307; Homo sapiens. [Genome view]
GeneID550.
KEGGhsa:550.
UCSCuc002smf.1. human.

Organism-specific databases

CTD550.
GeneCardsGC02M074607.
HGNCHGNC:891. AUP1.
HPAHPA007674.
MIM602434. gene.
PharmGKBPA25182.
GenAtlasSearch...

Phylogenomic databases

HOGENOMHBG714558.
HOVERGENHBG004321.
InParanoidQ9Y679.

Gene expression databases

ArrayExpressQ9Y679.
BgeeQ9Y679.
CleanExHS_AUP1.
GenevestigatorQ9Y679.
GermOnlineENSG00000115307. Homo sapiens.

Family and domain databases

InterProIPR003892. CUE.
[Graphical view]
PfamPF02845. CUE. 1 hit.
[Graphical view]
SMARTSM00546. CUE. 1 hit.
[Graphical view]
PROSITEPS51140. CUE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

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Entry information

Entry nameAUP1_HUMAN
AccessionPrimary (citable) accession number: Q9Y679
Secondary accession number(s): C0H5W8 expand/collapse secondary AC list , Q9H866, Q9UNQ6, Q9Y685
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: March 2, 2010
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Relevant documents

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents