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Q9Y679 (AUP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ancient ubiquitous protein 1
Gene names
Name:AUP1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length476 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. Ref.10

Subunit structure

Identified in a complex that contains SEL1L, OS9, FAF2/UBXD8, UBE2J1/UBC6E and AUP1. Interacts with the cytoplasmic tail of ITGA2B, ITGA1, ITGA2, ITGA5, ITGAV and ITGAM. Ref.7 Ref.10

Subcellular location

Endoplasmic reticulum membrane; Single-pass type III membrane protein; Cytoplasmic side Ref.7 Ref.10.

Tissue specificity

Detected in blood platelets and leukocytes (at protein level). Ubiquitous. Highly expressed in placenta, liver, kidney, skeletal muscle, heart and brain. Ref.7

Sequence similarities

Belongs to the AUP1 family.

Contains 1 CUE domain.

Sequence caution

The sequence AAH33646.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

OPRM1P353724EBI-1058701,EBI-2624570

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: Q9Y679-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: Q9Y679-2)

The sequence of this isoform differs from the canonical sequence as follows:
     114-179: Missing.
Isoform 3 (identifier: Q9Y679-3)

The sequence of this isoform differs from the canonical sequence as follows:
     114-179: Missing.
     426-439: FPSSGPVTPQPTAL → AFDACLMMMTPQAL
     440-476: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 476476Ancient ubiquitous protein 1
PRO_0000020765

Regions

Topological domain1 – 2020Lumenal Potential
Transmembrane21 – 4121Helical; Signal-anchor for type III membrane protein; Potential
Topological domain42 – 476435Cytoplasmic Potential
Domain362 – 40443CUE
Compositional bias160 – 1656Poly-Phe

Amino acid modifications

Modified residue11N-acetylmethionine Ref.14 Ref.15
Modified residue3541Phosphoserine Ref.8 Ref.9 Ref.12
Modified residue3581Phosphoserine Ref.9
Modified residue4291Phosphoserine Ref.9
Modified residue4331Phosphothreonine Ref.8 Ref.9

Natural variations

Alternative sequence114 – 17966Missing in isoform Short and isoform 3.
VSP_004133
Alternative sequence426 – 43914FPSSG…QPTAL → AFDACLMMMTPQAL in isoform 3.
VSP_036228
Alternative sequence440 – 47637Missing in isoform 3.
VSP_036229

Experimental info

Sequence conflict3541S → P in AAD43010. Ref.3

Secondary structure

......... 476
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: A6AF3AAD84AE86CB

FASTA47653,028
        10         20         30         40         50         60 
MELPSGPGPE RLFDSHRLPG DCFLLLVLLL YAPVGFCLLV LRLFLGIHVF LVSCALPDSV 

        70         80         90        100        110        120 
LRRFVVRTMC AVLGLVARQE DSGLRDHSVR VLISNHVTPF DHNIVNLLTT CSTVSESEAE 

       130        140        150        160        170        180 
SATGRFPGAQ LKAPLSPLAF RMEDTEALPL TPILYPTCQF FFFIFLNIFL LAFSSPGSQP 

       190        200        210        220        230        240 
LLNSPPSFVC WSRGFMEMNG RGELVESLKR FCASTRLPPT PLLLFPEEEA TNGREGLLRF 

       250        260        270        280        290        300 
SSWPFSIQDV VQPLTLQVQR PLVSVTVSDA SWVSELLWSL FVPFTVYQVR WLRPVHRQLG 

       310        320        330        340        350        360 
EANEEFALRV QQLVAKELGQ TGTRLTPADK AEHMKRQRHP RLRPQSAQSS FPPSPGPSPD 

       370        380        390        400        410        420 
VQLATLAQRV KEVLPHVPLG VIQRDLAKTG CVDLTITNLL EGAVAFMPED ITKGTQSLPT 

       430        440        450        460        470 
ASASKFPSSG PVTPQPTALT FAKSSWARQE SLQERKQALY EYARRRFTER RAQEAD 

« Hide

Isoform Short [UniParc].

Checksum: 1A06476225C5DFDC
Show »

FASTA41045,787
Isoform 3 [UniParc].

Checksum: AAB256D01055538E
Show »

FASTA37341,385

References

« Hide 'large scale' references
[1]"Human ancient ubiquitous protein AUP1 isoform gene."
Peng Y., Song H., Dai M., Huang Q., Mao Y., Zhang Q., Mao M., Fu G., Luo M., Chen J., Hu R.
Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
Tissue: Pituitary.
[2]"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. expand/collapse author list , Gu J., Chen S.-J., Chen Z.
Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
Tissue: Umbilical cord blood.
[3]"Novel genes expressed in hematopoietic stem/progenitor cells from myelodysplastic syndrome patients."
Gu J., Huang Q., Yu Y., Xu S., Han Z., Fu G., Zhou J., Wang Y., Huang C., Ren S., Tu Y., Chen Z.
Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
Tissue: Hematopoietic stem cell.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
Tissue: Eye.
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND SHORT).
Tissue: Eye and Skin.
[7]"Ancient ubiquitous protein 1 binds to the conserved membrane-proximal sequence of the cytoplasmic tail of the integrin alpha subunits that plays a crucial role in the inside-out signaling of alpha IIbbeta 3."
Kato A., Kawamata N., Tamayose K., Egashira M., Miura R., Fujimura T., Murayama K., Oshimi K.
J. Biol. Chem. 277:28934-28941(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ITGA2B; ITGA1; ITGA2; ITGA5; ITGAV AND ITGAM, TISSUE SPECIFICITY.
[8]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354 AND THR-433, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354; SER-358; SER-429 AND THR-433, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"SEL1L nucleates a protein complex required for dislocation of misfolded glycoproteins."
Mueller B., Klemm E.J., Spooner E., Claessen J.H., Ploegh H.L.
Proc. Natl. Acad. Sci. U.S.A. 105:12325-12330(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN A COMPLEX WITH SEL1L, SUBCELLULAR LOCATION, FUNCTION.
[11]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[13]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]"Solution structure of RSGI RUH-075, a human CUE domain."
RIKEN structural genomics initiative (RSGI)
Submitted (SEP-2007) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 358-411.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF100754 mRNA. Translation: AAD43018.1.
AF100753 mRNA. Translation: AAD43017.1.
AF100746 mRNA. Translation: AAD43010.1.
AF165515 mRNA. Translation: AAF86645.1.
AK023983 mRNA. Translation: BAB14753.1.
CH471053 Genomic DNA. Translation: EAW99623.1.
BC001658 mRNA. Translation: AAH01658.1.
BC033646 mRNA. Translation: AAH33646.2. Different initiation.
RefSeqNP_853553.1. NM_181575.3.
UniGeneHs.411480.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2EKFNMR-A358-411[»]
ProteinModelPortalQ9Y679.
SMRQ9Y679. Positions 359-411.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107031. 35 interactions.
IntActQ9Y679. 10 interactions.
MINTMINT-1190912.
STRING9606.ENSP00000366748.

PTM databases

PhosphoSiteQ9Y679.

Polymorphism databases

DMDM12643958.

Proteomic databases

PaxDbQ9Y679.
PRIDEQ9Y679.

Protocols and materials databases

DNASU550.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000377526; ENSP00000366748; ENSG00000115307. [Q9Y679-2]
ENST00000425118; ENSP00000403430; ENSG00000115307. [Q9Y679-3]
GeneID550.
KEGGhsa:550.
UCSCuc002smf.3. human. [Q9Y679-2]
uc002smh.3. human. [Q9Y679-1]

Organism-specific databases

CTD550.
GeneCardsGC02M074753.
HGNCHGNC:891. AUP1.
HPAHPA007674.
MIM602434. gene.
neXtProtNX_Q9Y679.
PharmGKBPA25182.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG271344.
HOGENOMHOG000034232.
HOVERGENHBG004321.
InParanoidQ9Y679.
OMAFLGIHVF.
OrthoDBEOG7HTHHJ.
PhylomeDBQ9Y679.
TreeFamTF313372.

Gene expression databases

BgeeQ9Y679.
CleanExHS_AUP1.
GenevestigatorQ9Y679.

Family and domain databases

InterProIPR003892. CUE.
[Graphical view]
PfamPF02845. CUE. 1 hit.
[Graphical view]
SMARTSM00546. CUE. 1 hit.
[Graphical view]
PROSITEPS51140. CUE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSAUP1. human.
EvolutionaryTraceQ9Y679.
GeneWikiAUP1.
GenomeRNAi550.
NextBio2273.
PROQ9Y679.
SOURCESearch...

Entry information

Entry nameAUP1_HUMAN
AccessionPrimary (citable) accession number: Q9Y679
Secondary accession number(s): C0H5W8 expand/collapse secondary AC list , Q9H866, Q9UNQ6, Q9Y685
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM