Q9Y679 (AUP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ancient ubiquitous protein 1

Gene names

Name:

AUP1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. Ref.10
Subunit structure	Identified in a complex that contains SEL1L, OS9, FAF2/UBXD8, UBE2J1/UBC6E and AUP1. Interacts with the cytoplasmic tail of ITGA2B, ITGA1, ITGA2, ITGA5, ITGAV and ITGAM. Ref.7 Ref.10
Subcellular location	Endoplasmic reticulum membrane; Single-pass type III membrane protein; Cytoplasmic side Ref.7 Ref.10.
Tissue specificity	Detected in blood platelets and leukocytes (at protein level). Ubiquitous. Highly expressed in placenta, liver, kidney, skeletal muscle, heart and brain. Ref.7
Sequence similarities	Belongs to the AUP1 family. Contains 1 CUE domain.
Sequence caution	The sequence AAH33646.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum Inferred from direct assay. Source: HPA endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay. Source: HPA
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATG12	O94817	1	EBI-1058701,EBI-746742
ILK	Q13418	1	EBI-1058701,EBI-747644
NDN	Q99608	1	EBI-1058701,EBI-718177
UBE2G2	P60604	1	EBI-1058701,EBI-1051028

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: Q9Y679-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: Q9Y679-2)

The sequence of this isoform differs from the canonical sequence as follows:
     114-179: Missing.

Isoform 3 (identifier: Q9Y679-3)

The sequence of this isoform differs from the canonical sequence as follows:
     114-179: Missing.
     426-439: FPSSGPVTPQPTAL → AFDACLMMMTPQAL
     440-476: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 476

476

Ancient ubiquitous protein 1

PRO_0000020765

Regions

Topological domain

1 – 20

20

Lumenal Potential

Transmembrane

21 – 41

21

Helical; Signal-anchor for type III membrane protein; Potential

Topological domain

42 – 476

435

Cytoplasmic Potential

Domain

362 – 404

43

CUE

Compositional bias

160 – 165

6

Poly-Phe

Amino acid modifications

Modified residue

354

1

Phosphoserine Ref.8 Ref.9 Ref.11

Modified residue

358

1

Phosphoserine Ref.9

Modified residue

429

1

Phosphoserine Ref.9

Modified residue

433

1

Phosphothreonine Ref.8 Ref.9

Natural variations

Alternative sequence

114 – 179

66

Missing in isoform Short and isoform 3.

VSP_004133

Alternative sequence

426 – 439

14

FPSSG…QPTAL → AFDACLMMMTPQAL in isoform 3.

VSP_036228

Alternative sequence

440 – 476

37

Missing in isoform 3.

VSP_036229

Experimental info

Sequence conflict

354

1

S → P in AAD43010. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

476

Helix Strand Turn

Details...

Helix

363 – 373

11

Beta strand

375 – 377

3

Helix

379 – 387

9

Helix

392 – 400

9

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified November 1, 1999. Version 1. Checksum: A6AF3AAD84AE86CB	FASTA	476	53,028
	10 20 30 40 50 60 MELPSGPGPE RLFDSHRLPG DCFLLLVLLL YAPVGFCLLV LRLFLGIHVF LVSCALPDSV 70 80 90 100 110 120 LRRFVVRTMC AVLGLVARQE DSGLRDHSVR VLISNHVTPF DHNIVNLLTT CSTVSESEAE 130 140 150 160 170 180 SATGRFPGAQ LKAPLSPLAF RMEDTEALPL TPILYPTCQF FFFIFLNIFL LAFSSPGSQP 190 200 210 220 230 240 LLNSPPSFVC WSRGFMEMNG RGELVESLKR FCASTRLPPT PLLLFPEEEA TNGREGLLRF 250 260 270 280 290 300 SSWPFSIQDV VQPLTLQVQR PLVSVTVSDA SWVSELLWSL FVPFTVYQVR WLRPVHRQLG 310 320 330 340 350 360 EANEEFALRV QQLVAKELGQ TGTRLTPADK AEHMKRQRHP RLRPQSAQSS FPPSPGPSPD 370 380 390 400 410 420 VQLATLAQRV KEVLPHVPLG VIQRDLAKTG CVDLTITNLL EGAVAFMPED ITKGTQSLPT 430 440 450 460 470 ASASKFPSSG PVTPQPTALT FAKSSWARQE SLQERKQALY EYARRRFTER RAQEAD
	Isoform Short [UniParc]. Checksum: 1A06476225C5DFDC	FASTA	410	45,787
	10 20 30 40 50 60 MELPSGPGPE RLFDSHRLPG DCFLLLVLLL YAPVGFCLLV LRLFLGIHVF LVSCALPDSV 70 80 90 100 110 120 LRRFVVRTMC AVLGLVARQE DSGLRDHSVR VLISNHVTPF DHNIVNLLTT CSTPLLNSPP 130 140 150 160 170 180 SFVCWSRGFM EMNGRGELVE SLKRFCASTR LPPTPLLLFP EEEATNGREG LLRFSSWPFS 190 200 210 220 230 240 IQDVVQPLTL QVQRPLVSVT VSDASWVSEL LWSLFVPFTV YQVRWLRPVH RQLGEANEEF 250 260 270 280 290 300 ALRVQQLVAK ELGQTGTRLT PADKAEHMKR QRHPRLRPQS AQSSFPPSPG PSPDVQLATL 310 320 330 340 350 360 AQRVKEVLPH VPLGVIQRDL AKTGCVDLTI TNLLEGAVAF MPEDITKGTQ SLPTASASKF 370 380 390 400 410 PSSGPVTPQP TALTFAKSSW ARQESLQERK QALYEYARRR FTERRAQEAD
	Isoform 3 [UniParc]. Checksum: AAB256D01055538E	FASTA	373	41,385
	10 20 30 40 50 60 MELPSGPGPE RLFDSHRLPG DCFLLLVLLL YAPVGFCLLV LRLFLGIHVF LVSCALPDSV 70 80 90 100 110 120 LRRFVVRTMC AVLGLVARQE DSGLRDHSVR VLISNHVTPF DHNIVNLLTT CSTPLLNSPP 130 140 150 160 170 180 SFVCWSRGFM EMNGRGELVE SLKRFCASTR LPPTPLLLFP EEEATNGREG LLRFSSWPFS 190 200 210 220 230 240 IQDVVQPLTL QVQRPLVSVT VSDASWVSEL LWSLFVPFTV YQVRWLRPVH RQLGEANEEF 250 260 270 280 290 300 ALRVQQLVAK ELGQTGTRLT PADKAEHMKR QRHPRLRPQS AQSSFPPSPG PSPDVQLATL 310 320 330 340 350 360 AQRVKEVLPH VPLGVIQRDL AKTGCVDLTI TNLLEGAVAF MPEDITKGTQ SLPTASASKA 370 FDACLMMMTP QAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human ancient ubiquitous protein AUP1 isoform gene." Peng Y., Song H., Dai M., Huang Q., Mao Y., Zhang Q., Mao M., Fu G., Luo M., Chen J., Hu R. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT). Tissue: Pituitary.
[2]	"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. , Gu J., Chen S.-J., Chen Z. Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT). Tissue: Umbilical cord blood.
[3]	"Novel genes expressed in hematopoietic stem/progenitor cells from myelodysplastic syndrome patients." Gu J., Huang Q., Yu Y., Xu S., Han Z., Fu G., Zhou J., Wang Y., Huang C., Ren S., Tu Y., Chen Z. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT). Tissue: Hematopoietic stem cell.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT). Tissue: Eye.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND SHORT). Tissue: Eye and Skin.
[7]	"Ancient ubiquitous protein 1 binds to the conserved membrane-proximal sequence of the cytoplasmic tail of the integrin alpha subunits that plays a crucial role in the inside-out signaling of alpha IIbbeta 3." Kato A., Kawamata N., Tamayose K., Egashira M., Miura R., Fujimura T., Murayama K., Oshimi K. J. Biol. Chem. 277:28934-28941(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ITGA2B; ITGA1; ITGA2; ITGA5; ITGAV AND ITGAM, TISSUE SPECIFICITY.
[8]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354 AND THR-433, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354; SER-358; SER-429 AND THR-433, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"SEL1L nucleates a protein complex required for dislocation of misfolded glycoproteins." Mueller B., Klemm E.J., Spooner E., Claessen J.H., Ploegh H.L. Proc. Natl. Acad. Sci. U.S.A. 105:12325-12330(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH SEL1L, SUBCELLULAR LOCATION, FUNCTION.
[11]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]	"Solution structure of RSGI RUH-075, a human CUE domain." RIKEN structural genomics initiative (RSGI) Submitted (SEP-2007) to the PDB data bank Cited for: STRUCTURE BY NMR OF 358-411.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF100754 mRNA. Translation: AAD43018.1.
AF100753 mRNA. Translation: AAD43017.1.
AF100746 mRNA. Translation: AAD43010.1.
AF165515 mRNA. Translation: AAF86645.1.
AK023983 mRNA. Translation: BAB14753.1.
CH471053 Genomic DNA. Translation: EAW99623.1.
BC001658 mRNA. Translation: AAH01658.1.
BC033646 mRNA. Translation: AAH33646.2. Different initiation.

IPI

IPI00001891.
IPI00215815.
IPI00917463.

RefSeq

NP_853553.1. NM_181575.3.

UniGene

Hs.411480.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EKF	NMR	-	A	358-411	[»]

ProteinModelPortal

Q9Y679.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9Y679. 6 interactions.

MINT

MINT-1190912.

STRING

9606.ENSP00000366748.

PTM databases

PhosphoSite

Q9Y679.

Polymorphism databases

DMDM

12643958.

Proteomic databases

PaxDb

Q9Y679.

PRIDE

Q9Y679.

Protocols and materials databases

DNASU

550.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000377526; ENSP00000366748; ENSG00000115307.
ENST00000425118; ENSP00000403430; ENSG00000115307.

GeneID

550.

KEGG

hsa:550.

UCSC

uc002smf.3. human.
uc002smh.3. human.

Organism-specific databases

CTD

550.

GeneCards

GC02M074753.

HGNC

HGNC:891. AUP1.

HPA

HPA007674.

MIM

602434. gene.

neXtProt

NX_Q9Y679.

PharmGKB

PA25182.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG271344.

HOGENOM

HOG000034232.

HOVERGEN

HBG004321.

InParanoid

Q9Y679.

Gene expression databases

ArrayExpress

Q9Y679.

Bgee

Q9Y679.

CleanEx

HS_AUP1.

Genevestigator

Q9Y679.

GermOnline

ENSG00000115307. Homo sapiens.

Family and domain databases

InterPro

IPR003892. CUE.
[Graphical view]

Pfam

PF02845. CUE. 1 hit.
[Graphical view]

SMART

SM00546. CUE. 1 hit.
[Graphical view]

PROSITE

PS51140. CUE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

AUP1. human.

EvolutionaryTrace

Q9Y679.

GenomeRNAi

550.

NextBio

2273.

SOURCE

Search...

Entry information

Entry name

AUP1_HUMAN

Accession

Primary (citable) accession number: Q9Y679
Secondary accession number(s): C0H5W8 , Q9H866, Q9UNQ6, Q9Y685

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.