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Protein

Ancient ubiquitous protein 1

Gene

AUP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome.1 Publication

GO - Molecular functioni

GO - Biological processi

  • ER-associated ubiquitin-dependent protein catabolic process Source: GO_Central
  • retrograde protein transport, ER to cytosol Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciZFISH:ENSG00000115307-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ancient ubiquitous protein 1
Gene namesi
Name:AUP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:891. AUP1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 20LumenalSequence analysisAdd BLAST20
Transmembranei21 – 41Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST21
Topological domaini42 – 476CytoplasmicSequence analysisAdd BLAST435

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • Hrd1p ubiquitin ligase ERAD-L complex Source: GO_Central
  • integral component of endoplasmic reticulum membrane Source: GO_Central
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000115307.
PharmGKBiPA25182.

Polymorphism and mutation databases

BioMutaiAUP1.
DMDMi12643958.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000207651 – 476Ancient ubiquitous protein 1Add BLAST476

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei5PhosphoserineCombined sources1
Modified residuei354PhosphoserineCombined sources1
Modified residuei358PhosphoserineCombined sources1
Modified residuei429PhosphoserineCombined sources1
Modified residuei433PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9Y679.
PaxDbiQ9Y679.
PeptideAtlasiQ9Y679.
PRIDEiQ9Y679.

PTM databases

iPTMnetiQ9Y679.
PhosphoSitePlusiQ9Y679.
SwissPalmiQ9Y679.

Expressioni

Tissue specificityi

Detected in blood platelets and leukocytes (at protein level). Ubiquitous. Highly expressed in placenta, liver, kidney, skeletal muscle, heart and brain.1 Publication

Gene expression databases

BgeeiENSG00000115307.
CleanExiHS_AUP1.
GenevisibleiQ9Y679. HS.

Organism-specific databases

HPAiHPA007674.

Interactioni

Subunit structurei

Identified in a complex that contains SEL1L, OS9, FAF2/UBXD8, UBE2J1/UBC6E and AUP1. Interacts with the cytoplasmic tail of ITGA2B, ITGA1, ITGA2, ITGA5, ITGAV and ITGAM.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
OPRM1P353724EBI-1058701,EBI-2624570

GO - Molecular functioni

Protein-protein interaction databases

BioGridi107031. 59 interactors.
IntActiQ9Y679. 22 interactors.
MINTiMINT-1190912.
STRINGi9606.ENSP00000366748.

Structurei

Secondary structure

1476
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi363 – 373Combined sources11
Beta strandi375 – 377Combined sources3
Helixi379 – 387Combined sources9
Helixi392 – 400Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EKFNMR-A358-411[»]
ProteinModelPortaliQ9Y679.
SMRiQ9Y679.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y679.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini362 – 404CUEPROSITE-ProRule annotationAdd BLAST43

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi160 – 165Poly-Phe6

Sequence similaritiesi

Belongs to the AUP1 family.Curated
Contains 1 CUE domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2898. Eukaryota.
ENOG410Y6KF. LUCA.
GeneTreeiENSGT00390000016110.
HOGENOMiHOG000034232.
HOVERGENiHBG004321.
InParanoidiQ9Y679.
KOiK19716.
OMAiLGIHVFL.
OrthoDBiEOG091G0CVF.
PhylomeDBiQ9Y679.
TreeFamiTF313372.

Family and domain databases

InterProiIPR003892. CUE.
[Graphical view]
PfamiPF02845. CUE. 1 hit.
[Graphical view]
SMARTiSM00546. CUE. 1 hit.
[Graphical view]
PROSITEiPS51140. CUE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q9Y679-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MELPSGPGPE RLFDSHRLPG DCFLLLVLLL YAPVGFCLLV LRLFLGIHVF
60 70 80 90 100
LVSCALPDSV LRRFVVRTMC AVLGLVARQE DSGLRDHSVR VLISNHVTPF
110 120 130 140 150
DHNIVNLLTT CSTVSESEAE SATGRFPGAQ LKAPLSPLAF RMEDTEALPL
160 170 180 190 200
TPILYPTCQF FFFIFLNIFL LAFSSPGSQP LLNSPPSFVC WSRGFMEMNG
210 220 230 240 250
RGELVESLKR FCASTRLPPT PLLLFPEEEA TNGREGLLRF SSWPFSIQDV
260 270 280 290 300
VQPLTLQVQR PLVSVTVSDA SWVSELLWSL FVPFTVYQVR WLRPVHRQLG
310 320 330 340 350
EANEEFALRV QQLVAKELGQ TGTRLTPADK AEHMKRQRHP RLRPQSAQSS
360 370 380 390 400
FPPSPGPSPD VQLATLAQRV KEVLPHVPLG VIQRDLAKTG CVDLTITNLL
410 420 430 440 450
EGAVAFMPED ITKGTQSLPT ASASKFPSSG PVTPQPTALT FAKSSWARQE
460 470
SLQERKQALY EYARRRFTER RAQEAD
Length:476
Mass (Da):53,028
Last modified:November 1, 1999 - v1
Checksum:iA6AF3AAD84AE86CB
GO
Isoform Short (identifier: Q9Y679-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     114-179: Missing.

Show »
Length:410
Mass (Da):45,787
Checksum:i1A06476225C5DFDC
GO
Isoform 3 (identifier: Q9Y679-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     114-179: Missing.
     426-439: FPSSGPVTPQPTAL → AFDACLMMMTPQAL
     440-476: Missing.

Show »
Length:373
Mass (Da):41,385
Checksum:iAAB256D01055538E
GO

Sequence cautioni

The sequence AAH33646 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti354S → P in AAD43010 (Ref. 3) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004133114 – 179Missing in isoform Short and isoform 3. 5 PublicationsAdd BLAST66
Alternative sequenceiVSP_036228426 – 439FPSSG…QPTAL → AFDACLMMMTPQAL in isoform 3. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_036229440 – 476Missing in isoform 3. 1 PublicationAdd BLAST37

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF100754 mRNA. Translation: AAD43018.1.
AF100753 mRNA. Translation: AAD43017.1.
AF100746 mRNA. Translation: AAD43010.1.
AF165515 mRNA. Translation: AAF86645.1.
AK023983 mRNA. Translation: BAB14753.1.
CH471053 Genomic DNA. Translation: EAW99623.1.
BC001658 mRNA. Translation: AAH01658.1.
BC033646 mRNA. Translation: AAH33646.2. Different initiation.
CCDSiCCDS42702.1. [Q9Y679-2]
RefSeqiNP_853553.1. NM_181575.4. [Q9Y679-2]
UniGeneiHs.411480.

Genome annotation databases

EnsembliENST00000377526; ENSP00000366748; ENSG00000115307. [Q9Y679-2]
ENST00000425118; ENSP00000403430; ENSG00000115307. [Q9Y679-3]
GeneIDi550.
KEGGihsa:550.
UCSCiuc002smf.4. human. [Q9Y679-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF100754 mRNA. Translation: AAD43018.1.
AF100753 mRNA. Translation: AAD43017.1.
AF100746 mRNA. Translation: AAD43010.1.
AF165515 mRNA. Translation: AAF86645.1.
AK023983 mRNA. Translation: BAB14753.1.
CH471053 Genomic DNA. Translation: EAW99623.1.
BC001658 mRNA. Translation: AAH01658.1.
BC033646 mRNA. Translation: AAH33646.2. Different initiation.
CCDSiCCDS42702.1. [Q9Y679-2]
RefSeqiNP_853553.1. NM_181575.4. [Q9Y679-2]
UniGeneiHs.411480.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EKFNMR-A358-411[»]
ProteinModelPortaliQ9Y679.
SMRiQ9Y679.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107031. 59 interactors.
IntActiQ9Y679. 22 interactors.
MINTiMINT-1190912.
STRINGi9606.ENSP00000366748.

PTM databases

iPTMnetiQ9Y679.
PhosphoSitePlusiQ9Y679.
SwissPalmiQ9Y679.

Polymorphism and mutation databases

BioMutaiAUP1.
DMDMi12643958.

Proteomic databases

EPDiQ9Y679.
PaxDbiQ9Y679.
PeptideAtlasiQ9Y679.
PRIDEiQ9Y679.

Protocols and materials databases

DNASUi550.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000377526; ENSP00000366748; ENSG00000115307. [Q9Y679-2]
ENST00000425118; ENSP00000403430; ENSG00000115307. [Q9Y679-3]
GeneIDi550.
KEGGihsa:550.
UCSCiuc002smf.4. human. [Q9Y679-1]

Organism-specific databases

CTDi550.
GeneCardsiAUP1.
HGNCiHGNC:891. AUP1.
HPAiHPA007674.
MIMi602434. gene.
neXtProtiNX_Q9Y679.
OpenTargetsiENSG00000115307.
PharmGKBiPA25182.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2898. Eukaryota.
ENOG410Y6KF. LUCA.
GeneTreeiENSGT00390000016110.
HOGENOMiHOG000034232.
HOVERGENiHBG004321.
InParanoidiQ9Y679.
KOiK19716.
OMAiLGIHVFL.
OrthoDBiEOG091G0CVF.
PhylomeDBiQ9Y679.
TreeFamiTF313372.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000115307-MONOMER.

Miscellaneous databases

ChiTaRSiAUP1. human.
EvolutionaryTraceiQ9Y679.
GeneWikiiAUP1.
GenomeRNAii550.
PROiQ9Y679.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000115307.
CleanExiHS_AUP1.
GenevisibleiQ9Y679. HS.

Family and domain databases

InterProiIPR003892. CUE.
[Graphical view]
PfamiPF02845. CUE. 1 hit.
[Graphical view]
SMARTiSM00546. CUE. 1 hit.
[Graphical view]
PROSITEiPS51140. CUE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAUP1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y679
Secondary accession number(s): C0H5W8
, Q9H866, Q9UNQ6, Q9Y685
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.