Reviewed, UniProtKB/Swiss-Prot Q9Y678 (COPG_HUMAN)

Names and origin

Protein names

Recommended name:
    Coatomer subunit gamma
Alternative name(s):
    Gamma-coat protein
      Short name=Gamma-COP

Gene names

Name:	COPG
Synonyms:	COPG1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	874 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors By similarity.
Subunit structure	Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Interacts with ZNF289/ARFGAP2 through its C-terminal appendage domain. Ref.4
Subcellular location	Cytoplasm By similarity. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasmic vesicle › COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it By similarity.
Sequence similarities	Belongs to the COPG family. Contains 4 HEAT repeats.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm Cytoplasmic vesicle Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Repeat
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	COPI coating of Golgi vesicle Inferred from Experiment. Source: Reactome intracellular protein transport Inferred from electronic annotation. Source: InterPro retrograde vesicle-mediated transport, Golgi to ER Inferred from Experiment. Source: Reactome
Cellular component	COPI vesicle coat Inferred from sequence or structural similarity. Source: UniProtKB cytosol Inferred from Experiment. Source: Reactome
Molecular function	protein binding Inferred from physical interaction. Source: IntAct structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BRF2	Q9HAW0	1	EBI-1049127,EBI-1055224
ILK	Q13418	1	EBI-1049127,EBI-747644
MAGED1	Q9Y5V3	1	EBI-1049127,EBI-716006
PHB2	Q99623	1	EBI-1049127,EBI-358348

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 874

874

Coatomer subunit gamma

PRO_0000193858

Regions

Repeat

64 – 101

38

HEAT 1

Repeat

283 – 320

38

HEAT 2

Repeat

322 – 355

34

HEAT 3

Repeat

356 – 392

37

HEAT 4

Region

609 – 874

266

Interaction with ZNF289/ARFGAP2

Natural variations

Natural variant

681

1

M → T: dbSNP rs15648.

VAR_054039

Experimental info

Mutagenesis

776

1

W → S: Loss of interaction with ZNF289/ARFGAP2. Ref.4

Secondary structure

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874

Helix Strand Turn

Details...

Helix

609 – 618

10

Helix

621 – 623

3

Beta strand

644 – 654

11

Beta strand

656 – 667

12

Beta strand

672 – 686

15

Beta strand

688 – 693

6

Beta strand

695 – 698

4

Beta strand

704 – 711

8

Beta strand

714 – 716

3

Beta strand

722 – 735

14

Turn

737 – 739

3

Beta strand

747 – 752

6

Beta strand

756 – 758

3

Helix

760 – 763

4

Beta strand

764 – 766

3

Helix

772 – 779

8

Beta strand

785 – 791

7

Helix

797 – 808

12

Turn

814 – 817

4

Beta strand

824 – 834

11

Turn

835 – 837

3

Beta strand

838 – 862

25

Helix

863 – 872

10

Sequences

	Sequence		Length	Mass (Da)
	Q9Y678-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: A2FAB492C598EC98	FASTA	874	97,718
	10 20 30 40 50 60 MLKKFDKKDE ESGGGSNPFQ HLEKSAVLQE ARVFNETPIN PRKCAHILTK ILYLINQGEH 70 80 90 100 110 120 LGTTEATEAF FAMTKLFQSN DPTLRRMCYL TIKEMSCIAE DVIIVTSSLT KDMTGKEDNY 130 140 150 160 170 180 RGPAVRALCQ ITDSTMLQAI ERYMKQAIVD KVPSVSSSAL VSSLHLLKCS FDVVKRWVNE 190 200 210 220 230 240 AQEAASSDNI MVQYHALGLL YHVRKNDRLA VNKMISKVTR HGLKSPFAYC MMIRVASKQL 250 260 270 280 290 300 EEEDGSRDSP LFDFIESCLR NKHEMVVYEA ASAIVNLPGC SAKELAPAVS VLQLFCSSPK 310 320 330 340 350 360 AALRYAAVRT LNKVAMKHPS AVTACNLDLE NLVTDSNRSI ATLAITTLLK TGSESSIDRL 370 380 390 400 410 420 MKQISSFMSE ISDEFKVVVV QAISALCQKY PRKHAVLMNF LFTMLREEGG FEYKRAIVDC 430 440 450 460 470 480 IISIIEENSE SKETGLSHLC EFIEDCEFTV LATRILHLLG QEGPKTTNPS KYIRFIYNRV 490 500 510 520 530 540 VLEHEEVRAG AVSALAKFGA QNEEMLPSIL VLLKRCVMDD DNEVRDRATF YLNVLEQKQK 550 560 570 580 590 600 ALNAGYILNG LTVSIPGLER ALQQYTLEPS EKPFDLKSVP LATAPMAEQR TESTPITAVK 610 620 630 640 650 660 QPEKVAATRQ EIFQEQLAAV PEFRGLGPLF KSSPEPVALT ESETEYVIRC TKHTFTNHMV 670 680 690 700 710 720 FQFDCTNTLN DQTLENVTVQ MEPTEAYEVL CYVPARSLPY NQPGTCYTLV ALPKEDPTAV 730 740 750 760 770 780 ACTFSCMMKF TVKDCDPTTG ETDDEGYEDE YVLEDLEVTV ADHIQKVMKL NFEAAWDEVG 790 800 810 820 830 840 DEFEKEETFT LSTIKTLEEA VGNIVKFLGM HPCERSDKVP DNKNTHTLLL AGVFRGGHDI 850 860 870 LVRSRLLLLD TVTMQVTARS LEELPVDIIL ASVG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed: 10931946] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[3]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[4]	"Gamma-COP appendage domain -- structure and function." Watson P.J., Frigerio G., Collins B.M., Duden R., Owen D.J. Traffic 5:79-88(2004) [PubMed: 14690497] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 608-874, INTERACTION WITH ZNF289, MUTAGENESIS OF TRP-776.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF100756 mRNA. Translation: AAD43020.1.
BC066650 mRNA. Translation: AAH66650.1.

IPI

IPI00783982.

RefSeq

NP_057212.1.

UniGene

Hs.518250

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1R4X	X-ray	1.90	A	608-874	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9Y678. 10 interactions.

Proteomic databases

PeptideAtlas

Q9Y678.

PRIDE

Q9Y678.

Genome annotation databases

Ensembl

ENSG00000181789. Homo sapiens. [Contig view]

GeneID

22820.

KEGG

hsa:22820.

UCSC

uc003els.1. human.

Organism-specific databases

GeneCards

GC03P130451.

H-InvDB

HIX0023057.

HGNC

HGNC:2236. COPG.

PharmGKB

PA26752.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

Q9Y678.

OMA

Q9Y678. LNFEAAW.

Enzyme and pathway databases

Reactome

REACT_11123. Membrane Trafficking.

Gene expression databases

ArrayExpress

Q9Y678.

Bgee

Q9Y678.

CleanEx

HS_COPG.

GermOnline

ENSG00000181789. Homo sapiens.

Family and domain databases

InterPro

IPR011989. ARM-like.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR015873. Clathrin_a/coatomer_app_sub_C.
IPR017106. Coatomer_gamma_subunit.
IPR014863. Coatomer_gsu_app.
IPR013040. Coatomer_gsu_app_Ig-like-sub.
[Graphical view]

Gene3D

G3DSA:3.30.310.30. AP2_A_adaptin_C. 1 hit.
G3DSA:1.25.10.10. ARM-like. 1 hit.
G3DSA:2.60.40.1480. Coatomer_gsu_app_Ig-like-sub. 1 hit.

Pfam

PF01602. Adaptin_N. 1 hit.
PF08752. Gamma-COP. 1 hit.
[Graphical view]

PIRSF

PIRSF037093. Coatomer_gamma_subunit. 1 hit.

PROSITE

PS50077. HEAT_REPEAT. False negative.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

43212.

Entry information

Entry name

COPG_HUMAN

Accession

Primary (citable) accession number: Q9Y678

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	November 1, 1999
Last modified:	July 7, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)