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Protein

Dolichyl-phosphate beta-glucosyltransferase

Gene

ALG5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

UDP-glucose + dolichyl phosphate = UDP + dolichyl beta-D-glucosyl phosphate.

Pathway: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • dolichyl-phosphate beta-glucosyltransferase activity Source: UniProtKB-EC
  • oligosaccharyl transferase activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_22346. Synthesis of dolichyl-phosphate-glucose.
SABIO-RKQ9Y673.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-phosphate beta-glucosyltransferase (EC:2.4.1.117)
Short name:
DolP-glucosyltransferase
Alternative name(s):
Asparagine-linked glycosylation protein 5 homolog
Gene namesi
Name:ALG5
ORF Names:HSPC149
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 13

Organism-specific databases

HGNCiHGNC:20266. ALG5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence Analysis
Transmembranei8 – 2821Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini29 – 324296LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: Reactome
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134977026.

Polymorphism and mutation databases

BioMutaiALG5.
DMDMi27734217.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324Dolichyl-phosphate beta-glucosyltransferasePRO_0000059098Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi281 – 2811N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9Y673.
PaxDbiQ9Y673.
PRIDEiQ9Y673.

PTM databases

PhosphoSiteiQ9Y673.

Expressioni

Tissue specificityi

Expressed in pancreas, placenta, liver, heart, brain, kidney, skeletal muscle, and lung.

Gene expression databases

BgeeiQ9Y673.
CleanExiHS_ALG5.
GenevisibleiQ9Y673. HS.

Organism-specific databases

HPAiHPA007989.

Interactioni

Protein-protein interaction databases

BioGridi118935. 7 interactions.
STRINGi9606.ENSP00000239891.

Structurei

3D structure databases

ProteinModelPortaliQ9Y673.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0463.
GeneTreeiENSGT00550000074941.
HOGENOMiHOG000283250.
HOVERGENiHBG024330.
InParanoidiQ9Y673.
KOiK00729.
OMAiVRMGVFS.
OrthoDBiEOG7XPZ6G.
PhylomeDBiQ9Y673.
TreeFamiTF314844.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y673-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPLLLQLAV LGAALAAAAL VLISIVAFTT ATKMPALHRH EEEKFFLNAK
60 70 80 90 100
GQKETLPSIW DSPTKQLSVV VPSYNEEKRL PVMMDEALSY LEKRQKRDPA
110 120 130 140 150
FTYEVIVVDD GSKDQTSKVA FKYCQKYGSD KVRVITLVKN RGKGGAIRMG
160 170 180 190 200
IFSSRGEKIL MADADGATKF PDVEKLEKGL NDLQPWPNQM AIACGSRAHL
210 220 230 240 250
EKESIAQRSY FRTLLMYGFH FLVWFLCVKG IRDTQCGFKL FTREAASRTF
260 270 280 290 300
SSLHVERWAF DVELLYIAQF FKIPIAEIAV NWTEIEGSKL VPFWSWLQMG
310 320
KDLLFIRLRY LTGAWRLEQT RKMN
Length:324
Mass (Da):36,946
Last modified:November 1, 1999 - v1
Checksum:i44E1826BAB5B36CB
GO
Isoform 2 (identifier: Q9Y673-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     95-124: Missing.

Note: No experimental confirmation available.
Show »
Length:294
Mass (Da):33,525
Checksum:i3A5E796B95FDC6C6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei95 – 12430Missing in isoform 2. 1 PublicationVSP_041019Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF102850 mRNA. Translation: AAD41465.1.
AF161498 mRNA. Translation: AAF29113.1.
AK299085 mRNA. Translation: BAG61149.1.
AL138706 Genomic DNA. Translation: CAI14011.1.
CH471075 Genomic DNA. Translation: EAX08578.1.
BC012531 mRNA. Translation: AAH12531.1.
CCDSiCCDS45033.1. [Q9Y673-2]
CCDS9361.1. [Q9Y673-1]
PIRiT51776.
RefSeqiNP_001135836.1. NM_001142364.1. [Q9Y673-2]
NP_037470.1. NM_013338.4. [Q9Y673-1]
UniGeneiHs.507769.

Genome annotation databases

EnsembliENST00000239891; ENSP00000239891; ENSG00000120697. [Q9Y673-1]
ENST00000443765; ENSP00000390533; ENSG00000120697. [Q9Y673-2]
GeneIDi29880.
KEGGihsa:29880.
UCSCiuc001uvy.3. human. [Q9Y673-1]
uc010teq.2. human. [Q9Y673-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF102850 mRNA. Translation: AAD41465.1.
AF161498 mRNA. Translation: AAF29113.1.
AK299085 mRNA. Translation: BAG61149.1.
AL138706 Genomic DNA. Translation: CAI14011.1.
CH471075 Genomic DNA. Translation: EAX08578.1.
BC012531 mRNA. Translation: AAH12531.1.
CCDSiCCDS45033.1. [Q9Y673-2]
CCDS9361.1. [Q9Y673-1]
PIRiT51776.
RefSeqiNP_001135836.1. NM_001142364.1. [Q9Y673-2]
NP_037470.1. NM_013338.4. [Q9Y673-1]
UniGeneiHs.507769.

3D structure databases

ProteinModelPortaliQ9Y673.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118935. 7 interactions.
STRINGi9606.ENSP00000239891.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

PTM databases

PhosphoSiteiQ9Y673.

Polymorphism and mutation databases

BioMutaiALG5.
DMDMi27734217.

Proteomic databases

MaxQBiQ9Y673.
PaxDbiQ9Y673.
PRIDEiQ9Y673.

Protocols and materials databases

DNASUi29880.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000239891; ENSP00000239891; ENSG00000120697. [Q9Y673-1]
ENST00000443765; ENSP00000390533; ENSG00000120697. [Q9Y673-2]
GeneIDi29880.
KEGGihsa:29880.
UCSCiuc001uvy.3. human. [Q9Y673-1]
uc010teq.2. human. [Q9Y673-2]

Organism-specific databases

CTDi29880.
GeneCardsiGC13M037523.
HGNCiHGNC:20266. ALG5.
HPAiHPA007989.
MIMi604565. gene.
neXtProtiNX_Q9Y673.
PharmGKBiPA134977026.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0463.
GeneTreeiENSGT00550000074941.
HOGENOMiHOG000283250.
HOVERGENiHBG024330.
InParanoidiQ9Y673.
KOiK00729.
OMAiVRMGVFS.
OrthoDBiEOG7XPZ6G.
PhylomeDBiQ9Y673.
TreeFamiTF314844.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_22346. Synthesis of dolichyl-phosphate-glucose.
SABIO-RKQ9Y673.

Miscellaneous databases

ChiTaRSiALG5. human.
GenomeRNAii29880.
NextBioi52401.
PROiQ9Y673.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Y673.
CleanExiHS_ALG5.
GenevisibleiQ9Y673. HS.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A mutation in the human ortholog of the Saccharomyces cerevisiae ALG6 gene causes carbohydrate-deficient glycoprotein syndrome type-Ic."
    Imbach T., Burda P., Kuhnert P., Wevers R.A., Aebi M., Berger E.G., Hennet T.
    Proc. Natl. Acad. Sci. U.S.A. 96:6982-6987(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
    Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
    , Gu J., Chen S.-J., Chen Z.
    Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Umbilical cord blood.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  4. "The DNA sequence and analysis of human chromosome 13."
    Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
    Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Prostate.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiALG5_HUMAN
AccessioniPrimary (citable) accession number: Q9Y673
Secondary accession number(s): B4DR37, Q5TBA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: November 1, 1999
Last modified: June 24, 2015
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.