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Protein

Heparan sulfate glucosamine 3-O-sulfotransferase 3B1

Gene

HS3ST3B1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N-unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Catalyzes the O-sulfation of glucosamine in IdoUA2S-GlcNS and also in IdoUA2S-GlcNH2. The substrate-specific O-sulfation generates an enzyme-modified heparan sulfate which acts as a binding receptor to Herpes simplex virus-1 (HSV-1) and permits its entry. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate.1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei228 – 2281PAPSBy similarity
Binding sitei236 – 2361PAPSBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi147 – 1515PAPSBy similarity
Nucleotide bindingi353 – 3575PAPSBy similarity

GO - Molecular functioni

GO - Biological processi

  • glycosaminoglycan biosynthetic process Source: Reactome
  • heparan sulfate proteoglycan biosynthetic process Source: ProtInc
  • heparan sulfate proteoglycan biosynthetic process, enzymatic modification Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciMetaCyc:HS04884-MONOMER.
ReactomeiR-HSA-2022928. HS-GAG biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Heparan sulfate glucosamine 3-O-sulfotransferase 3B1 (EC:2.8.2.30)
Alternative name(s):
Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3B1
Short name:
3-OST-3B
Short name:
Heparan sulfate 3-O-sulfotransferase 3B1
Short name:
h3-OST-3B
Gene namesi
Name:HS3ST3B1
Synonyms:3OST3B1, HS3ST3B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:5198. HS3ST3B1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3232CytoplasmicSequence analysisAdd
BLAST
Transmembranei33 – 5321Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini54 – 390337LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • Golgi membrane Source: Reactome
  • integral component of plasma membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29471.

Polymorphism and mutation databases

BioMutaiHS3ST3B1.
DMDMi61214548.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 390390Heparan sulfate glucosamine 3-O-sulfotransferase 3B1PRO_0000085219Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi258 – 2581N-linked (GlcNAc...)Sequence analysis
Glycosylationi329 – 3291N-linked (GlcNAc...)Sequence analysis
Disulfide bondi336 ↔ 348By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9Y662.
PeptideAtlasiQ9Y662.
PRIDEiQ9Y662.

PTM databases

iPTMnetiQ9Y662.
PhosphoSiteiQ9Y662.

Expressioni

Tissue specificityi

Ubiquitous. Most abundant in liver and placenta, followed by heart and kidney.1 Publication

Gene expression databases

BgeeiQ9Y662.
CleanExiHS_HS3ST3B1.
GenevisibleiQ9Y662. HS.

Interactioni

Protein-protein interaction databases

BioGridi115278. 1 interaction.
STRINGi9606.ENSP00000354213.

Structurei

3D structure databases

ProteinModelPortaliQ9Y662.
SMRiQ9Y662. Positions 133-390.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni169 – 1757Substrate bindingBy similarity
Regioni200 – 2034Substrate bindingBy similarity
Regioni268 – 2692Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the sulfotransferase 1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3704. Eukaryota.
ENOG410XS59. LUCA.
GeneTreeiENSGT00760000119023.
HOGENOMiHOG000036663.
HOVERGENiHBG053377.
InParanoidiQ9Y662.
KOiK07809.
OMAiLAMWPAG.
OrthoDBiEOG7PS1GM.
PhylomeDBiQ9Y662.
TreeFamiTF350755.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Y662-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQRLSGGRS CLDVPGRLLP QPPPPPPPVR RKLALLFAML CVWLYMFLYS
60 70 80 90 100
CAGSCAAAPG LLLLGSGSRA AHDPPALATA PDGTPPRLPF RAPPATPLAS
110 120 130 140 150
GKEMAEGAAS PEEQSPEVPD SPSPISSFFS GSGSKQLPQA IIIGVKKGGT
160 170 180 190 200
RALLEFLRVH PDVRAVGAEP HFFDRSYDKG LAWYRDLMPR TLDGQITMEK
210 220 230 240 250
TPSYFVTREA PARISAMSKD TKLIVVVRDP VTRAISDYTQ TLSKRPDIPT
260 270 280 290 300
FESLTFKNRT AGLIDTSWSA IQIGIYAKHL EHWLRHFPIR QMLFVSGERL
310 320 330 340 350
ISDPAGELGR VQDFLGLKRI ITDKHFYFNK TKGFPCLKKA EGSSRPHCLG
360 370 380 390
KTKGRTHPEI DREVVRRLRE FYRPFNLKFY QMTGHDFGWD
Length:390
Mass (Da):43,324
Last modified:November 1, 1999 - v1
Checksum:i5C54C3E9836BC614
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF105377 mRNA. Translation: AAD30209.1.
AK023723 mRNA. Translation: BAG51220.1.
CH471108 Genomic DNA. Translation: EAW89953.1.
CH471108 Genomic DNA. Translation: EAW89954.1.
BC063301 mRNA. Translation: AAH63301.1.
BC069664 mRNA. Translation: AAH69664.1.
BC069725 mRNA. Translation: AAH69725.1.
CCDSiCCDS11167.1.
RefSeqiNP_006032.1. NM_006041.2.
UniGeneiHs.48384.

Genome annotation databases

EnsembliENST00000360954; ENSP00000354213; ENSG00000125430.
ENST00000466596; ENSP00000436078; ENSG00000125430.
GeneIDi9953.
KEGGihsa:9953.
UCSCiuc002goh.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF105377 mRNA. Translation: AAD30209.1.
AK023723 mRNA. Translation: BAG51220.1.
CH471108 Genomic DNA. Translation: EAW89953.1.
CH471108 Genomic DNA. Translation: EAW89954.1.
BC063301 mRNA. Translation: AAH63301.1.
BC069664 mRNA. Translation: AAH69664.1.
BC069725 mRNA. Translation: AAH69725.1.
CCDSiCCDS11167.1.
RefSeqiNP_006032.1. NM_006041.2.
UniGeneiHs.48384.

3D structure databases

ProteinModelPortaliQ9Y662.
SMRiQ9Y662. Positions 133-390.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115278. 1 interaction.
STRINGi9606.ENSP00000354213.

PTM databases

iPTMnetiQ9Y662.
PhosphoSiteiQ9Y662.

Polymorphism and mutation databases

BioMutaiHS3ST3B1.
DMDMi61214548.

Proteomic databases

PaxDbiQ9Y662.
PeptideAtlasiQ9Y662.
PRIDEiQ9Y662.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000360954; ENSP00000354213; ENSG00000125430.
ENST00000466596; ENSP00000436078; ENSG00000125430.
GeneIDi9953.
KEGGihsa:9953.
UCSCiuc002goh.2. human.

Organism-specific databases

CTDi9953.
GeneCardsiHS3ST3B1.
HGNCiHGNC:5198. HS3ST3B1.
MIMi604058. gene.
neXtProtiNX_Q9Y662.
PharmGKBiPA29471.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3704. Eukaryota.
ENOG410XS59. LUCA.
GeneTreeiENSGT00760000119023.
HOGENOMiHOG000036663.
HOVERGENiHBG053377.
InParanoidiQ9Y662.
KOiK07809.
OMAiLAMWPAG.
OrthoDBiEOG7PS1GM.
PhylomeDBiQ9Y662.
TreeFamiTF350755.

Enzyme and pathway databases

BioCyciMetaCyc:HS04884-MONOMER.
ReactomeiR-HSA-2022928. HS-GAG biosynthesis.

Miscellaneous databases

GeneWikiiHS3ST3B1.
GenomeRNAii9953.
PROiQ9Y662.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Y662.
CleanExiHS_HS3ST3B1.
GenevisibleiQ9Y662. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Multiple isoforms of heparan sulfate D-glucosaminyl 3-O-sulfotransferase. Isolation, characterization, and expression of human cDNAs and identification of distinct genomic loci."
    Shworak N.W., Liu J., Petros L.M., Zhang L., Kobayashi M., Copeland N.G., Jenkins N.A., Rosenberg R.D.
    J. Biol. Chem. 274:5170-5184(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Liver.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  5. "A novel role for 3-O-sulfated heparan sulfate in herpes simplex virus 1 entry."
    Shukla D., Liu J., Blaiklock P., Shworak N.W., Bai X., Esko J.D., Cohen G.H., Eisenberg R.J., Rosenberg R.D., Spear P.G.
    Cell 99:13-22(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN HSV-1 ENTRY.
  6. "Expression of heparan sulfate D-glucosaminyl 3-O-sulfotransferase isoforms reveals novel substrate specificities."
    Liu J., Shworak N.W., Sinay P., Schwartz J.J., Zhang L., Fritze L.M.S., Rosenberg R.D.
    J. Biol. Chem. 274:5185-5192(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiHS3SB_HUMAN
AccessioniPrimary (citable) accession number: Q9Y662
Secondary accession number(s): B3KN58, D3DTS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1999
Last modified: July 6, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.