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Protein

Heparan sulfate glucosamine 3-O-sulfotransferase 4

Gene

HS3ST4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N-unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate (By similarity).By similarity

Catalytic activityi

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei288 – 2881PAPSBy similarity
Binding sitei296 – 2961PAPSBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi207 – 2115PAPSBy similarity
Nucleotide bindingi413 – 4175PAPSBy similarity

GO - Molecular functioni

  • [heparan sulfate]-glucosamine 3-sulfotransferase 1 activity Source: UniProtKB

GO - Biological processi

  • heparan sulfate proteoglycan metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiR-HSA-2022928. HS-GAG biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Heparan sulfate glucosamine 3-O-sulfotransferase 4 (EC:2.8.2.23)
Alternative name(s):
Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 4
Short name:
3-OST-4
Short name:
Heparan sulfate 3-O-sulfotransferase 4
Short name:
h3-OST-4
Gene namesi
Name:HS3ST4
Synonyms:3OST4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:5200. HS3ST4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3434CytoplasmicSequence analysisAdd
BLAST
Transmembranei35 – 5925Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini60 – 456397LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29473.

Polymorphism and mutation databases

BioMutaiHS3ST4.
DMDMi527504083.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 456456Heparan sulfate glucosamine 3-O-sulfotransferase 4PRO_0000085221Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi105 – 1051N-linked (GlcNAc...)Sequence analysis
Glycosylationi178 – 1781N-linked (GlcNAc...)Sequence analysis
Glycosylationi318 – 3181N-linked (GlcNAc...)Sequence analysis
Glycosylationi389 – 3891N-linked (GlcNAc...)Sequence analysis
Disulfide bondi396 ↔ 408By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9Y661.
PRIDEiQ9Y661.

Expressioni

Tissue specificityi

Brain-specific.1 Publication

Gene expression databases

BgeeiQ9Y661.
CleanExiHS_HS3ST4.
GenevisibleiQ9Y661. HS.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000330606.

Structurei

3D structure databases

ProteinModelPortaliQ9Y661.
SMRiQ9Y661. Positions 191-450.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni229 – 2357Substrate bindingBy similarity
Regioni260 – 2634Substrate bindingBy similarity
Regioni328 – 3292Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the sulfotransferase 1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3704. Eukaryota.
ENOG410XS59. LUCA.
GeneTreeiENSGT00760000119023.
HOGENOMiHOG000036663.
HOVERGENiHBG053377.
InParanoidiQ9Y661.
KOiK09678.
OMAiNASHGEP.
OrthoDBiEOG7PS1GM.
PhylomeDBiQ9Y661.
TreeFamiTF350755.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Y661-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARWPAPPPP PPPPPPLAAP PPPGASAKGP PARKLLFMCT LSLSVTYLCY
60 70 80 90 100
SLLGGSGSLQ FPLALQESPG AAAEPPPSPP PPSLLPTPVR LGAPSQPPAP
110 120 130 140 150
PPLDNASHGE PPEPPEQPAA PGTDGWGLPS GGGGAQDAWL RTPLAPSEMI
160 170 180 190 200
TAQSALPERE AQESSTTDED LAGRRAANGS SERGGAVSTP DYGEKKLPQA
210 220 230 240 250
LIIGVKKGGT RALLEAIRVH PDVRAVGVEP HFFDRNYEKG LEWYRNVMPK
260 270 280 290 300
TLDGQITMEK TPSYFVTNEA PKRIHSMAKD IKLIVVVRNP VTRAISDYTQ
310 320 330 340 350
TLSKKPEIPT FEVLAFKNRT LGLIDASWSA IRIGIYALHL ENWLQYFPLS
360 370 380 390 400
QILFVSGERL IVDPAGEMAK VQDFLGLKRV VTEKHFYFNK TKGFPCLKKP
410 420 430 440 450
EDSSAPRCLG KSKGRTHPRI DPDVIHRLRK FYKPFNLMFY QMTGQDFQWE

QEEGDK
Length:456
Mass (Da):49,799
Last modified:July 24, 2013 - v3
Checksum:iA1BA47F9817F6B38
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti383 – 3831E → K in AAD30210 (PubMed:9988767).Curated
Sequence conflicti383 – 3831E → K in AAS58324 (Ref. 2) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti136 – 1361Q → R.3 Publications
Corresponds to variant rs2943336 [ dbSNP | Ensembl ].
VAR_069580

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF105378 mRNA. Translation: AAD30210.2.
AY476736 Genomic DNA. Translation: AAS58324.1.
AC092141 Genomic DNA. No translation available.
AC093511 Genomic DNA. No translation available.
AL834283 mRNA. Translation: CAD38957.1.
CCDSiCCDS53995.1.
RefSeqiNP_006031.2. NM_006040.2.
UniGeneiHs.655275.

Genome annotation databases

EnsembliENST00000331351; ENSP00000330606; ENSG00000182601.
GeneIDi9951.
KEGGihsa:9951.
UCSCiuc002dof.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF105378 mRNA. Translation: AAD30210.2.
AY476736 Genomic DNA. Translation: AAS58324.1.
AC092141 Genomic DNA. No translation available.
AC093511 Genomic DNA. No translation available.
AL834283 mRNA. Translation: CAD38957.1.
CCDSiCCDS53995.1.
RefSeqiNP_006031.2. NM_006040.2.
UniGeneiHs.655275.

3D structure databases

ProteinModelPortaliQ9Y661.
SMRiQ9Y661. Positions 191-450.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000330606.

Polymorphism and mutation databases

BioMutaiHS3ST4.
DMDMi527504083.

Proteomic databases

PaxDbiQ9Y661.
PRIDEiQ9Y661.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000331351; ENSP00000330606; ENSG00000182601.
GeneIDi9951.
KEGGihsa:9951.
UCSCiuc002dof.3. human.

Organism-specific databases

CTDi9951.
GeneCardsiHS3ST4.
H-InvDBHIX0012909.
HGNCiHGNC:5200. HS3ST4.
MIMi604059. gene.
neXtProtiNX_Q9Y661.
PharmGKBiPA29473.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3704. Eukaryota.
ENOG410XS59. LUCA.
GeneTreeiENSGT00760000119023.
HOGENOMiHOG000036663.
HOVERGENiHBG053377.
InParanoidiQ9Y661.
KOiK09678.
OMAiNASHGEP.
OrthoDBiEOG7PS1GM.
PhylomeDBiQ9Y661.
TreeFamiTF350755.

Enzyme and pathway databases

ReactomeiR-HSA-2022928. HS-GAG biosynthesis.

Miscellaneous databases

ChiTaRSiHS3ST4. human.
GenomeRNAii9951.
NextBioi37550.
PROiQ9Y661.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Y661.
CleanExiHS_HS3ST4.
GenevisibleiQ9Y661. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Multiple isoforms of heparan sulfate D-glucosaminyl 3-O-sulfotransferase. Isolation, characterization, and expression of human cDNAs and identification of distinct genomic loci."
    Shworak N.W., Liu J., Petros L.M., Zhang L., Kobayashi M., Copeland N.G., Jenkins N.A., Rosenberg R.D.
    J. Biol. Chem. 274:5170-5184(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT ARG-136.
    Tissue: Brain.
  2. "Neuronal 3-O-sulfotransferases exhibit trigeminal ganglion expression, confer entry of HSV-1, and may explain disease patterns."
    Lawrence R., Yabe T., Hajmohammadi S., Rhodes J., McNeely M., Liu J., Lamperti E.D., Toselli P.A., Spear P.G., Rosenberg R.D., Shworak N.W.
    Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-136.
  3. "The sequence and analysis of duplication-rich human chromosome 16."
    Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
    , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
    Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 122-456, VARIANT ARG-136.
    Tissue: Brain.

Entry informationi

Entry nameiHS3S4_HUMAN
AccessioniPrimary (citable) accession number: Q9Y661
Secondary accession number(s): Q5QI42, Q8NDC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: July 24, 2013
Last modified: November 11, 2015
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.