Q9Y657 (SPIN1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spindlin-1

Alternative name(s):
Ovarian cancer-related protein

Gene names

Name:	SPIN1
Synonyms:	OCR, SPIN

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in cell-cycle regulation during the transition from gamete to embryo By similarity.
Subunit structure	Homodimer; may form higher-order oligomers. Ref.13
Subcellular location	Nucleus Ref.3.
Post-translational modification	Phosphorylated during oocyte meiotic maturation By similarity.
Sequence similarities	Belongs to the SPIN/STSY family.
Sequence caution	The sequence AAD43035.1 differs from that shown. Reason: Frameshift at position 21. The sequence AAG38112.1 differs from that shown. Reason: Erroneous initiation. The sequence AAG48367.1 differs from that shown. Reason: Erroneous initiation. The sequence CAB66653.1 differs from that shown. Reason: Erroneous termination at position 171. Translated as Glu. The sequence CAG38515.1 differs from that shown. Reason: Erroneous termination at position 171. Translated as Glu.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat
Ligand	DNA-binding
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW gamete generation Inferred from electronic annotation. Source: InterPro multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay. Source: LIFEdb spindle Inferred from electronic annotation. Source: Compara
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW methylated histone residue binding Inferred from direct assay PubMed 21029866. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 262

262

Spindlin-1

PRO_0000181367

Regions

Region

53 – 116

64

Tudor-like domain 1

Region

132 – 193

62

Tudor-like domain 2

Region

213 – 262

50

Tudor-like domain 3

Amino acid modifications

Modified residue

124

1

Phosphoserine Ref.11 Ref.12

Modified residue

199

1

Phosphoserine Ref.11

Natural variations

Natural variant

221

1

A → P.
Corresponds to variant rs34794905 [ dbSNP | Ensembl ].

VAR_053690

Experimental info

Sequence conflict

49

1

P → S in BAG52466. Ref.6

Secondary structure

1

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262

Helix Strand Turn

Details...

Beta strand

57 – 63

7

Beta strand

69 – 79

11

Beta strand

87 – 91

5

Beta strand

98 – 100

3

Turn

102 – 104

3

Beta strand

108 – 113

6

Helix

126 – 132

7

Beta strand

136 – 142

7

Beta strand

144 – 146

3

Beta strand

148 – 158

11

Beta strand

160 – 162

3

Beta strand

165 – 170

6

Beta strand

173 – 179

7

Helix

181 – 186

6

Beta strand

190 – 192

3

Beta strand

217 – 222

6

Turn

223 – 225

3

Beta strand

226 – 235

10

Beta strand

237 – 239

3

Beta strand

242 – 247

6

Beta strand

254 – 257

4

Sequences

	Sequence		Length	Mass (Da)
	Q9Y657 [UniParc]. Last modified April 18, 2006. Version 3. Checksum: 49F86CBCC7A0AA01	FASTA	262	29,601
	10 20 30 40 50 60 MKTPFGKTPG QRSRADAGHA GVSANMMKKR TSHKKHRSSV GPSKPVSQPR RNIVGCRIQH 70 80 90 100 110 120 GWKEGNGPVT QWKGTVLDQV PVNPSLYLIK YDGFDCVYGL ELNKDERVSA LEVLPDRVAT 130 140 150 160 170 180 SRISDAHLAD TMIGKAVEHM FETEDGSKDE WRGMVLARAP VMNTWFYITY EKDPVLYMYQ 190 200 210 220 230 240 LLDDYKEGDL RIMPDSNDSP PAEREPGEVV DSLVGKQVEY AKEDGSKRTG MVIHQVEAKP 250 260 SVYFIKFDDD FHIYVYDLVK TS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Peng Y., Song H., Dai M., Huang Q., Mao Y., Zhang Q., Mao M., Fu G., Luo M., Chen J., Hu R. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pituitary.
[2]	"Cloning, characterization and mapping of human SPIN to human chromosome 9q22.1-22.3." Zhang H.L., Yu L., Wang X., Chen Z., Tu Q., Chen J.Q., Ding J.B., Gao J., Zhao S.Y. Chin. Sci. Bull. 45:909-914(2000) Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Spindlin1, a novel nuclear protein with a role in the transformation of NIH3T3 cells." Gao Y., Yue W., Zhang P., Li L., Xie X., Yuan H., Chen L., Liu D., Yan F., Pei X. Biochem. Biophys. Res. Commun. 335:343-350(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION. Tissue: Ovarian carcinoma.
[4]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Fetal kidney.
[5]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Amygdala, Hippocampus and Trachea.
[7]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[10]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-225.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124 AND SER-199, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"Structure of human spindlin1. Tandem tudor-like domains for cell cycle regulation." Zhao Q., Qin L., Jiang F., Wu B., Yue W., Xu F., Rong Z., Yuan H., Xie X., Gao Y., Bai C., Bartlam M., Pei X., Rao Z. J. Biol. Chem. 282:647-656(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 26-262 IN COMPLEX WITH PHOSPHATE IONS, DNA-BINDING, SUBUNIT, TUDOR-LIKE DOMAINS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF106682 mRNA. Translation: AAD43035.1. Frameshift.
AF087864 mRNA. Translation: AAG48367.1. Different initiation.
AF317228 mRNA. Translation: AAG38112.1. Different initiation.
AL136719 mRNA. Translation: CAB66653.1. Different termination.
BT007314 mRNA. Translation: AAP35978.1.
AK092017 mRNA. Translation: BAG52466.1.
AK289691 mRNA. Translation: BAF82380.1.
AK290009 mRNA. Translation: BAF82698.1.
AK315854 mRNA. Translation: BAF98745.1.
AL353748 Genomic DNA. Translation: CAH72406.1.
CH471089 Genomic DNA. Translation: EAW62753.1.
BC013571 mRNA. No translation available.
BC114515 mRNA. Translation: AAI14516.1.
BC114565 mRNA. Translation: AAI14566.1.
CR533484 mRNA. Translation: CAG38515.1. Different termination.

IPI

IPI00550655.

RefSeq

NP_006708.2. NM_006717.2.

UniGene

Hs.146804.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2NS2	X-ray	2.20	A/B	26-262	[»]
4H75	X-ray	2.10	A	27-262	[»]

ProteinModelPortal

Q9Y657.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9Y657. 5 interactions.

MINT

MINT-1428826.

STRING

9606.ENSP00000365019.

PTM databases

PhosphoSite

Q9Y657.

Polymorphism databases

DMDM

93141317.

Proteomic databases

PaxDb

Q9Y657.

PRIDE

Q9Y657.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000375859; ENSP00000365019; ENSG00000106723.
ENST00000541629; ENSP00000441864; ENSG00000106723.

GeneID

10927.

KEGG

hsa:10927.

UCSC

uc004apy.3. human.

Organism-specific databases

CTD

10927.

GeneCards

GC09P091003.

HGNC

HGNC:11243. SPIN1.

HPA

HPA000162.

MIM

609936. gene.

neXtProt

NX_Q9Y657.

PharmGKB

PA162404504.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG40069.

HOGENOM

HOG000293367.

HOVERGEN

HBG000686.

InParanoid

Q9Y657.

OMA

DFLIYVY.

OrthoDB

EOG4RV2S3.

Gene expression databases

Bgee

Q9Y657.

CleanEx

HS_SPIN1.

Genevestigator

Q9Y657.

GermOnline

ENSG00000106723. Homo sapiens.

Family and domain databases

InterPro

IPR003671. Spin_Ssty.
[Graphical view]

Pfam

PF02513. Spin-Ssty. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

SPIN1. human.

EvolutionaryTrace

Q9Y657.

GenomeRNAi

10927.

NextBio

41511.

SOURCE

Search...

Entry information

Entry name

SPIN1_HUMAN

Accession

Primary (citable) accession number: Q9Y657
Secondary accession number(s): A8K0X6 , B3KRQ4, Q7KZJ8, Q9GZT2, Q9H0N7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	April 18, 2006
Last modified:	May 1, 2013
This is version 94 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.