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Q9Y624 (JAM1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 148. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Junctional adhesion molecule A

Short name=JAM-A
Alternative name(s):
Junctional adhesion molecule 1
Short name=JAM-1
Platelet F11 receptor
Platelet adhesion molecule 1
Short name=PAM-1
CD_antigen=CD321
Gene names
Name:F11R
Synonyms:JAM1, JCAM
ORF Names:UNQ264/PRO301
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length299 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly By similarity. Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.

Subunit structure

Interacts with the ninth PDZ domain of MPDZ. Interacts with the first PDZ domain of PARD3. The association between PARD3 and PARD6B probably disrupts this interaction. Interacts with the orthoreovirus sigma-1 capsid protein By similarity. Ref.10

Subcellular location

Cell junctiontight junction. Cell membrane; Single-pass type I membrane protein. Note: Localized at tight junctions of both epithelial and endothelial cells. Ref.3

Post-translational modification

N-glycosylated. Ref.7

Sequence similarities

Belongs to the immunoglobulin superfamily.

Contains 2 Ig-like V-type (immunoglobulin-like) domains.

Ontologies

Keywords
   Biological processHost-virus interaction
   Cellular componentCell junction
Cell membrane
Membrane
Tight junction
   DomainImmunoglobulin domain
Repeat
Signal
Transmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processblood coagulation

Traceable author statement. Source: Reactome

cell adhesion

Inferred from electronic annotation. Source: Ensembl

cell junction assembly

Traceable author statement. Source: Reactome

cell-cell junction organization

Traceable author statement. Source: Reactome

epithelial cell differentiation

Inferred from electronic annotation. Source: Ensembl

extracellular matrix organization

Traceable author statement. Source: Reactome

inflammatory response

Traceable author statement Ref.1. Source: ProtInc

leukocyte migration

Traceable author statement. Source: Reactome

tight junction assembly

Traceable author statement. Source: Reactome

transforming growth factor beta receptor signaling pathway

Traceable author statement. Source: Reactome

viral process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcell junction

Inferred from direct assay. Source: HPA

cell-cell junction

Traceable author statement Ref.1. Source: ProtInc

extracellular vesicular exosome

Inferred from direct assay PubMed 19199708PubMed 20458337PubMed 23376485. Source: UniProt

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

microtubule cytoskeleton

Inferred from direct assay. Source: HPA

plasma membrane

Inferred from direct assay. Source: LIFEdb

tight junction

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 17057644. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PARD3Q8TEW02EBI-742600,EBI-81968

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Ref.7 Ref.8 Ref.9
Chain28 – 299272Junctional adhesion molecule A
PRO_0000015066

Regions

Topological domain28 – 238211Extracellular Potential
Transmembrane239 – 25921Helical; Potential
Topological domain260 – 29940Cytoplasmic Potential
Domain28 – 12598Ig-like V-type 1
Domain135 – 22894Ig-like V-type 2

Amino acid modifications

Modified residue2811Phosphoserine Ref.13
Modified residue2841Phosphoserine Ref.12 Ref.13 Ref.15 Ref.17
Modified residue2871Phosphoserine Ref.13
Glycosylation1851N-linked (GlcNAc...) Ref.14
Glycosylation1911N-linked (GlcNAc...) Ref.14
Disulfide bond50 ↔ 109
Disulfide bond153 ↔ 212

Secondary structure

................................................ 299
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9Y624 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: D95DE2FEA23D2851

FASTA29932,583
        10         20         30         40         50         60 
MGTKAQVERK LLCLFILAIL LCSLALGSVT VHSSEPEVRI PENNPVKLSC AYSGFSSPRV 

        70         80         90        100        110        120 
EWKFDQGDTT RLVCYNNKIT ASYEDRVTFL PTGITFKSVT REDTGTYTCM VSEEGGNSYG 

       130        140        150        160        170        180 
EVKVKLIVLV PPSKPTVNIP SSATIGNRAV LTCSEQDGSP PSEYTWFKDG IVMPTNPKST 

       190        200        210        220        230        240 
RAFSNSSYVL NPTTGELVFD PLSASDTGEY SCEARNGYGT PMTSNAVRME AVERNVGVIV 

       250        260        270        280        290 
AAVLVTLILL GILVFGIWFA YSRGHFDRTK KGTSSKKVIY SQPSARSEGE FKQTSSFLV 

« Hide

References

« Hide 'large scale' references
[1]"Combined treatment of TNF-alpha and IFN-gamma causes redistribution of junctional adhesion molecule in human endothelial cells."
Ozaki H., Ishii K., Horiuchi H., Arai H., Kawamoto T., Okawa K., Iwamatsu A., Kita T.
J. Immunol. 163:553-557(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning of the human platelet F11 receptor: a cell adhesion molecule member of the immunoglobulin superfamily involved in platelet aggregation."
Sobocka M.B., Sobocki T., Banerjee P., Weiss C., Rushbrook J.I., Norin A.J., Hartwig J., Salifu M.O., Markell M.S., Babinska A., Ehrlich Y.H., Kornecki E.
Blood 95:2600-2609(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Characterization and chromosomal localization of JAM-1, a platelet receptor for a stimulatory monoclonal antibody."
Naik U.P., Naik M.U., Eckfeld K., Martin-DeLeon P., Spychala J.
J. Cell Sci. 114:539-547(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
[4]"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs."
Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. expand/collapse author list , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A.
Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[5]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary.
[7]"Mechanisms of platelet activation by a stimulatory antibody: cross-linking of a novel platelet receptor for monoclonal antibody F11 with the Fc gamma RII receptor."
Naik U.P., Ehrlich Y.H., Kornecki E.
Biochem. J. 310:155-162(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-103 AND 123-130, GLYCOSYLATION.
[8]"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Zhang Z., Henzel W.J.
Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-42.
[9]"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-39.
Tissue: Platelet.
[10]"Junctional adhesion molecule (JAM) binds to PAR-3: a possible mechanism for the recruitment of PAR-3 to tight junctions."
Itoh M., Sasaki H., Furuse M., Ozaki H., Kita T., Tsukita S.
J. Cell Biol. 154:491-497(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MPDZ.
[11]"Leukocyte-endothelial-cell interactions in leukocyte transmigration and the inflammatory response."
Muller W.A.
Trends Immunol. 24:327-334(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW, NOMENCLATURE.
[12]"Phosphoproteome of resting human platelets."
Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A.
J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Platelet.
[13]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-281; SER-284 AND SER-287, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[14]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-185 AND ASN-191.
Tissue: Liver.
[15]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[16]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[17]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[18]"Crystal structure of human junctional adhesion molecule 1: implications for reovirus binding."
Prota A.E., Campbell J.A., Schelling P., Forrest J.C., Watson M.J., Peters T.R., Aurrand-Lions M.A., Imhof B.A., Dermody T.S., Stehle T.
Proc. Natl. Acad. Sci. U.S.A. 100:5366-5371(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 28-233.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF111713 mRNA. Translation: AAD42050.1.
AF207907 mRNA. Translation: AAF22829.1.
AF172398 mRNA. Translation: AAD48877.1.
AL136649 mRNA. Translation: CAB66584.1.
AY358896 mRNA. Translation: AAQ89255.1.
BC001533 mRNA. Translation: AAH01533.1.
CCDSCCDS1213.1.
PIRS56749. A59406.
RefSeqNP_058642.1. NM_016946.4.
UniGeneHs.517293.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1NBQX-ray2.90A/B27-233[»]
3EOYX-ray3.40G/H/I/J/K/L28-129[»]
3TSZX-ray2.50B288-299[»]
ProteinModelPortalQ9Y624.
SMRQ9Y624. Positions 25-233.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119153. 10 interactions.
IntActQ9Y624. 5 interactions.
MINTMINT-154235.
STRING9606.ENSP00000289779.

PTM databases

PhosphoSiteQ9Y624.

Polymorphism databases

DMDM10720061.

Proteomic databases

MaxQBQ9Y624.
PaxDbQ9Y624.
PeptideAtlasQ9Y624.
PRIDEQ9Y624.

Protocols and materials databases

DNASU50848.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000368026; ENSP00000357005; ENSG00000158769.
GeneID50848.
KEGGhsa:50848.
UCSCuc001fxf.4. human.

Organism-specific databases

CTD50848.
GeneCardsGC01M160965.
GC01M160975.
HGNCHGNC:14685. F11R.
HPACAB004671.
HPA043616.
MIM605721. gene.
neXtProtNX_Q9Y624.
PharmGKBPA29991.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG150281.
HOVERGENHBG000518.
InParanoidQ9Y624.
KOK06089.
OMASCSYSGF.
OrthoDBEOG7MH0Z1.
PhylomeDBQ9Y624.
TreeFamTF343984.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_111155. Cell-Cell communication.
REACT_116125. Disease.
REACT_118779. Extracellular matrix organization.
REACT_604. Hemostasis.

Gene expression databases

ArrayExpressQ9Y624.
BgeeQ9Y624.
CleanExHS_F11R.
GenevestigatorQ9Y624.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
IPR003596. Ig_V-set_subgr.
[Graphical view]
PfamPF07686. V-set. 1 hit.
[Graphical view]
SMARTSM00408. IGc2. 1 hit.
SM00406. IGv. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSF11R. human.
EvolutionaryTraceQ9Y624.
GeneWikiF11_receptor.
GenomeRNAi50848.
NextBio53317.
PROQ9Y624.
SOURCESearch...

Entry information

Entry nameJAM1_HUMAN
AccessionPrimary (citable) accession number: Q9Y624
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: July 9, 2014
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries