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Q9Y624

- JAM1_HUMAN

UniProt

Q9Y624 - JAM1_HUMAN

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Protein

Junctional adhesion molecule A

Gene
F11R, JAM1, JCAM, UNQ264/PRO301
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly By similarity. Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. blood coagulation Source: Reactome
  2. cell adhesion Source: Ensembl
  3. cell-cell junction organization Source: Reactome
  4. cell junction assembly Source: Reactome
  5. epithelial cell differentiation Source: Ensembl
  6. extracellular matrix organization Source: Reactome
  7. inflammatory response Source: ProtInc
  8. leukocyte migration Source: Reactome
  9. tight junction assembly Source: Reactome
  10. transforming growth factor beta receptor signaling pathway Source: Reactome
  11. viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction

Enzyme and pathway databases

ReactomeiREACT_12051. Cell surface interactions at the vascular wall.
REACT_120726. TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition).
REACT_13552. Integrin cell surface interactions.
REACT_19373. Tight junction interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Junctional adhesion molecule A
Short name:
JAM-A
Alternative name(s):
Junctional adhesion molecule 1
Short name:
JAM-1
Platelet F11 receptor
Platelet adhesion molecule 1
Short name:
PAM-1
CD_antigen: CD321
Gene namesi
Name:F11R
Synonyms:JAM1, JCAM
ORF Names:UNQ264/PRO301
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:14685. F11R.

Subcellular locationi

Cell junctiontight junction. Cell membrane; Single-pass type I membrane protein
Note: Localized at tight junctions of both epithelial and endothelial cells.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 238211Extracellular Reviewed predictionAdd
BLAST
Transmembranei239 – 25921Helical; Reviewed predictionAdd
BLAST
Topological domaini260 – 29940Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. cell-cell junction Source: ProtInc
  2. cell junction Source: HPA
  3. extracellular vesicular exosome Source: UniProt
  4. integral component of membrane Source: UniProtKB-KW
  5. microtubule cytoskeleton Source: HPA
  6. plasma membrane Source: LIFEdb
  7. tight junction Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29991.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 27273 PublicationsAdd
BLAST
Chaini28 – 299272Junctional adhesion molecule APRO_0000015066Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi50 ↔ 109
Disulfide bondi153 ↔ 212
Glycosylationi185 – 1851N-linked (GlcNAc...)1 Publication
Glycosylationi191 – 1911N-linked (GlcNAc...)1 Publication
Modified residuei281 – 2811Phosphoserine1 Publication
Modified residuei284 – 2841Phosphoserine4 Publications
Modified residuei287 – 2871Phosphoserine1 Publication

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9Y624.
PaxDbiQ9Y624.
PeptideAtlasiQ9Y624.
PRIDEiQ9Y624.

PTM databases

PhosphoSiteiQ9Y624.

Expressioni

Gene expression databases

ArrayExpressiQ9Y624.
BgeeiQ9Y624.
CleanExiHS_F11R.
GenevestigatoriQ9Y624.

Organism-specific databases

HPAiCAB004671.
HPA043616.

Interactioni

Subunit structurei

Interacts with the ninth PDZ domain of MPDZ. Interacts with the first PDZ domain of PARD3. The association between PARD3 and PARD6B probably disrupts this interaction. Interacts with the orthoreovirus sigma-1 capsid protein By similarity.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PARD3Q8TEW02EBI-742600,EBI-81968

Protein-protein interaction databases

BioGridi119153. 10 interactions.
IntActiQ9Y624. 5 interactions.
MINTiMINT-154235.
STRINGi9606.ENSP00000289779.

Structurei

Secondary structure

1
299
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi30 – 323
Beta strandi36 – 405
Beta strandi47 – 493
Beta strandi51 – 544
Beta strandi56 – 6611
Beta strandi69 – 757
Beta strandi76 – 794
Turni81 – 866
Beta strandi88 – 903
Beta strandi93 – 953
Helixi101 – 1033
Beta strandi105 – 1139
Beta strandi123 – 1253
Beta strandi128 – 1303
Beta strandi140 – 1445
Beta strandi149 – 1513
Beta strandi163 – 1686
Beta strandi177 – 1826
Turni192 – 1943
Beta strandi197 – 2015
Helixi204 – 2063
Beta strandi210 – 2156
Beta strandi217 – 2193
Beta strandi230 – 2323

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NBQX-ray2.90A/B27-233[»]
3EOYX-ray3.40G/H/I/J/K/L28-129[»]
3TSZX-ray2.50B288-299[»]
ProteinModelPortaliQ9Y624.
SMRiQ9Y624. Positions 25-233.

Miscellaneous databases

EvolutionaryTraceiQ9Y624.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 12598Ig-like V-type 1Add
BLAST
Domaini135 – 22894Ig-like V-type 2Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG150281.
HOVERGENiHBG000518.
InParanoidiQ9Y624.
KOiK06089.
OMAiSCSYSGF.
OrthoDBiEOG7MH0Z1.
PhylomeDBiQ9Y624.
TreeFamiTF343984.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
IPR003596. Ig_V-set_subgr.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00408. IGc2. 1 hit.
SM00406. IGv. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Y624-1 [UniParc]FASTAAdd to Basket

« Hide

MGTKAQVERK LLCLFILAIL LCSLALGSVT VHSSEPEVRI PENNPVKLSC    50
AYSGFSSPRV EWKFDQGDTT RLVCYNNKIT ASYEDRVTFL PTGITFKSVT 100
REDTGTYTCM VSEEGGNSYG EVKVKLIVLV PPSKPTVNIP SSATIGNRAV 150
LTCSEQDGSP PSEYTWFKDG IVMPTNPKST RAFSNSSYVL NPTTGELVFD 200
PLSASDTGEY SCEARNGYGT PMTSNAVRME AVERNVGVIV AAVLVTLILL 250
GILVFGIWFA YSRGHFDRTK KGTSSKKVIY SQPSARSEGE FKQTSSFLV 299
Length:299
Mass (Da):32,583
Last modified:November 1, 1999 - v1
Checksum:iD95DE2FEA23D2851
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF111713 mRNA. Translation: AAD42050.1.
AF207907 mRNA. Translation: AAF22829.1.
AF172398 mRNA. Translation: AAD48877.1.
AL136649 mRNA. Translation: CAB66584.1.
AY358896 mRNA. Translation: AAQ89255.1.
BC001533 mRNA. Translation: AAH01533.1.
CCDSiCCDS1213.1.
PIRiA59406. S56749.
RefSeqiNP_058642.1. NM_016946.4.
UniGeneiHs.517293.

Genome annotation databases

EnsembliENST00000368026; ENSP00000357005; ENSG00000158769.
GeneIDi50848.
KEGGihsa:50848.
UCSCiuc001fxf.4. human.

Polymorphism databases

DMDMi10720061.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF111713 mRNA. Translation: AAD42050.1 .
AF207907 mRNA. Translation: AAF22829.1 .
AF172398 mRNA. Translation: AAD48877.1 .
AL136649 mRNA. Translation: CAB66584.1 .
AY358896 mRNA. Translation: AAQ89255.1 .
BC001533 mRNA. Translation: AAH01533.1 .
CCDSi CCDS1213.1.
PIRi A59406. S56749.
RefSeqi NP_058642.1. NM_016946.4.
UniGenei Hs.517293.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1NBQ X-ray 2.90 A/B 27-233 [» ]
3EOY X-ray 3.40 G/H/I/J/K/L 28-129 [» ]
3TSZ X-ray 2.50 B 288-299 [» ]
ProteinModelPortali Q9Y624.
SMRi Q9Y624. Positions 25-233.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 119153. 10 interactions.
IntActi Q9Y624. 5 interactions.
MINTi MINT-154235.
STRINGi 9606.ENSP00000289779.

PTM databases

PhosphoSitei Q9Y624.

Polymorphism databases

DMDMi 10720061.

Proteomic databases

MaxQBi Q9Y624.
PaxDbi Q9Y624.
PeptideAtlasi Q9Y624.
PRIDEi Q9Y624.

Protocols and materials databases

DNASUi 50848.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000368026 ; ENSP00000357005 ; ENSG00000158769 .
GeneIDi 50848.
KEGGi hsa:50848.
UCSCi uc001fxf.4. human.

Organism-specific databases

CTDi 50848.
GeneCardsi GC01M160965.
GC01M160975.
HGNCi HGNC:14685. F11R.
HPAi CAB004671.
HPA043616.
MIMi 605721. gene.
neXtProti NX_Q9Y624.
PharmGKBi PA29991.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG150281.
HOVERGENi HBG000518.
InParanoidi Q9Y624.
KOi K06089.
OMAi SCSYSGF.
OrthoDBi EOG7MH0Z1.
PhylomeDBi Q9Y624.
TreeFami TF343984.

Enzyme and pathway databases

Reactomei REACT_12051. Cell surface interactions at the vascular wall.
REACT_120726. TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition).
REACT_13552. Integrin cell surface interactions.
REACT_19373. Tight junction interactions.

Miscellaneous databases

ChiTaRSi F11R. human.
EvolutionaryTracei Q9Y624.
GeneWikii F11_receptor.
GenomeRNAii 50848.
NextBioi 53317.
PROi Q9Y624.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9Y624.
Bgeei Q9Y624.
CleanExi HS_F11R.
Genevestigatori Q9Y624.

Family and domain databases

Gene3Di 2.60.40.10. 2 hits.
InterProi IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
IPR003596. Ig_V-set_subgr.
[Graphical view ]
Pfami PF07686. V-set. 1 hit.
[Graphical view ]
SMARTi SM00408. IGc2. 1 hit.
SM00406. IGv. 1 hit.
[Graphical view ]
PROSITEi PS50835. IG_LIKE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Combined treatment of TNF-alpha and IFN-gamma causes redistribution of junctional adhesion molecule in human endothelial cells."
    Ozaki H., Ishii K., Horiuchi H., Arai H., Kawamoto T., Okawa K., Iwamatsu A., Kita T.
    J. Immunol. 163:553-557(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning of the human platelet F11 receptor: a cell adhesion molecule member of the immunoglobulin superfamily involved in platelet aggregation."
    Sobocka M.B., Sobocki T., Banerjee P., Weiss C., Rushbrook J.I., Norin A.J., Hartwig J., Salifu M.O., Markell M.S., Babinska A., Ehrlich Y.H., Kornecki E.
    Blood 95:2600-2609(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Characterization and chromosomal localization of JAM-1, a platelet receptor for a stimulatory monoclonal antibody."
    Naik U.P., Naik M.U., Eckfeld K., Martin-DeLeon P., Spychala J.
    J. Cell Sci. 114:539-547(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.
  7. "Mechanisms of platelet activation by a stimulatory antibody: cross-linking of a novel platelet receptor for monoclonal antibody F11 with the Fc gamma RII receptor."
    Naik U.P., Ehrlich Y.H., Kornecki E.
    Biochem. J. 310:155-162(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-103 AND 123-130, GLYCOSYLATION.
  8. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
    Zhang Z., Henzel W.J.
    Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-42.
  9. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
    Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
    Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-39.
    Tissue: Platelet.
  10. "Junctional adhesion molecule (JAM) binds to PAR-3: a possible mechanism for the recruitment of PAR-3 to tight junctions."
    Itoh M., Sasaki H., Furuse M., Ozaki H., Kita T., Tsukita S.
    J. Cell Biol. 154:491-497(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MPDZ.
  11. "Leukocyte-endothelial-cell interactions in leukocyte transmigration and the inflammatory response."
    Muller W.A.
    Trends Immunol. 24:327-334(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW, NOMENCLATURE.
  12. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Platelet.
  13. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-281; SER-284 AND SER-287, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  14. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
    Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
    J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-185 AND ASN-191.
    Tissue: Liver.
  15. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  16. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  17. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  18. Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 28-233.

Entry informationi

Entry nameiJAM1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y624
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: September 3, 2014
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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