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Protein

Myosin-4

Gene

MYH4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Muscle contraction.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi179 – 1868ATPSequence Analysis

GO - Molecular functioni

  • ATPase activity Source: BHF-UCL
  • ATP binding Source: UniProtKB-KW
  • double-stranded RNA binding Source: MGI
  • microfilament motor activity Source: UniProtKB

GO - Biological processi

  • actin filament-based movement Source: UniProtKB
  • ATP metabolic process Source: BHF-UCL
  • membrane organization Source: Reactome
  • muscle contraction Source: BHF-UCL
  • muscle filament sliding Source: BHF-UCL
  • response to activity Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_147867. Translocation of GLUT4 to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-4
Alternative name(s):
Myosin heavy chain 2b
Short name:
MyHC-2b
Myosin heavy chain 4
Myosin heavy chain IIb
Short name:
MyHC-IIb
Myosin heavy chain, skeletal muscle, fetal
Gene namesi
Name:MYH4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:7574. MYH4.

Subcellular locationi

GO - Cellular componenti

  • muscle myosin complex Source: BHF-UCL
  • myofibril Source: BHF-UCL
  • myosin filament Source: UniProtKB-KW
  • sarcomere Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Thick filament

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31371.

Polymorphism and mutation databases

BioMutaiMYH4.
DMDMi224471840.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19391939Myosin-4PRO_0000123398Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei130 – 1301N6,N6,N6-trimethyllysineSequence Analysis
Cross-linki1813 – 1813Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Methylation, Ubl conjugation

Proteomic databases

MaxQBiQ9Y623.
PaxDbiQ9Y623.
PRIDEiQ9Y623.

2D gel databases

UCD-2DPAGEQ9Y623.

PTM databases

PhosphoSiteiQ9Y623.

Expressioni

Gene expression databases

BgeeiQ9Y623.
CleanExiHS_MYH4.
GenevisibleiQ9Y623. HS.

Organism-specific databases

HPAiHPA001349.

Interactioni

Subunit structurei

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Protein-protein interaction databases

BioGridi110707. 11 interactions.
IntActiQ9Y623. 4 interactions.
MINTiMINT-5003666.
STRINGi9606.ENSP00000255381.

Structurei

3D structure databases

ProteinModelPortaliQ9Y623.
SMRiQ9Y623. Positions 4-967.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini86 – 782697Myosin motorAdd
BLAST
Domaini785 – 81430IQPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni659 – 68123Actin-bindingBy similarityAdd
BLAST
Regioni761 – 77515Actin-bindingBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili843 – 19391097Sequence AnalysisAdd
BLAST

Domaini

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Sequence similaritiesi

Contains 1 IQ domain.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5022.
GeneTreeiENSGT00760000118919.
HOGENOMiHOG000173959.
HOVERGENiHBG004704.
InParanoidiQ9Y623.
KOiK10352.
OMAiDDQVFSM.
OrthoDBiEOG7RBZ7G.
PhylomeDBiQ9Y623.
TreeFamiTF314375.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 2 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Y623-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSDSEMAIF GEAAPFLRKS EKERIEAQNK PFDAKTSVFV VDPKESYVKA
60 70 80 90 100
IVQSREGGKV TAKTEAGATV TVKEDQVFSM NPPKYDKIED MAMMTHLHEP
110 120 130 140 150
AVLYNLKERY AAWMIYTYSG LFCVTVNPYK WLPVYNPEVV TAYRGKKRQE
160 170 180 190 200
APPHIFSISD NAYQFMLTDR ENQSILITGE SGAGKTVNTK RVIQYFATIA
210 220 230 240 250
VTGEKKKEEP ASGKMQGTLE DQIISANPLL EAFGNAKTVR NDNSSRFGKF
260 270 280 290 300
IRIHFGATGK LASADIETYL LEKSRVTFQL KAERSYHIFY QILSNKKPEL
310 320 330 340 350
IEMLLITTNP YDFAFVSQGE ITVPSIDDQE ELMATDSAVD ILGFTADEKV
360 370 380 390 400
AIYKLTGAVM HYGNMKFKQK QREEQAEPDG TEVADKAAYL TSLNSADLLK
410 420 430 440 450
SLCYPRVKVG NEFVTKGQTV QQVYNAVGAL AKAIYEKMFL WMVTRINQQL
460 470 480 490 500
DTKQPRQYFI GVLDIAGFEI FDFNSLEQLC INFTNEKLQQ FFNHHMFVLE
510 520 530 540 550
QEEYKKEGIE WEFIDFGMDL AACIELIEKP MGIFSILEEE CMFPKATDTS
560 570 580 590 600
FKNKLYEQHL GKSNNFQKPK PAKGKPEAHF SLVHYAGTVD YNIAGWLDKN
610 620 630 640 650
KDPLNETVVG LYQKSAMKTL AFLFSGAQTA EAEGGGGKKG GKKKGSSFQT
660 670 680 690 700
VSALFRENLN KLMTNLRSTH PHFVRCIIPN ETKTPGAMEH ELVLHQLRCN
710 720 730 740 750
GVLEGIRICR KGFPSRILYA DFKQRYKVLN ASAIPEGQFI DSKKASEKLL
760 770 780 790 800
GSIEIDHTQY KFGHTKVFFK AGLLGTLEEM RDEKLAQLIT RTQAICRGFL
810 820 830 840 850
MRVEFRKMME RRESIFCIQY NIRAFMNVKH WPWMKLYFKI KPLLKSAETE
860 870 880 890 900
KEMANMKEEF EKTKEELAKT EAKRKELEEK MVTLMQEKND LQLQVQAEAD
910 920 930 940 950
ALADAEERCD QLIKTKIQLE AKIKEVTERA EDEEEINAEL TAKKRKLEDE
960 970 980 990 1000
CSELKKDIDD LELTLAKVEK EKHATENKVK NLTEEMAGLD ETIAKLTKEK
1010 1020 1030 1040 1050
KALQEAHQQT LDDLQMEEDK VNTLTKAKTK LEQQVDDLEG SLEQEKKLCM
1060 1070 1080 1090 1100
DLERAKRKLE GDLKLAQEST MDTENDKQQL NEKLKKKEFE MSNLQGKIED
1110 1120 1130 1140 1150
EQALAIQLQK KIKELQARIE ELEEEIEAER ASRAKAEKQR SDLSRELEEI
1160 1170 1180 1190 1200
SERLEEAGGA TSAQIEMNKK REAEFQKMRR DLEESTLQHE ATAAALRKKH
1210 1220 1230 1240 1250
ADSVAELGEQ IDSLQRVKQK LEKEKSELKM EINDLASNME TVSKAKANFE
1260 1270 1280 1290 1300
KMCRTLEDQL SEIKTKEEEQ QRLINELSAQ KARLHTESGE FSRQLDEKDA
1310 1320 1330 1340 1350
MVSQLSRGKQ AFTQQIEELK RQLEEETKAK STLAHALQSA RHDCDLLREQ
1360 1370 1380 1390 1400
YEEEQEAKAE LQRGMSKANS EVAQWRTKYE TDAIQRTEEL EEAKKKLAQR
1410 1420 1430 1440 1450
LQDAEEHVEA VNSKCASLEK TKQRLQNEVE DLMIDVERSN AACIALDKKQ
1460 1470 1480 1490 1500
RNFDKVLAEW KQKYEETQAE LEASQKESRS LSTELFKVKN AYEESLDHLE
1510 1520 1530 1540 1550
TLKRENKNLQ QEISDLTEQI AEGGKHIHEL EKVKKQLDHE KSELQTSLEE
1560 1570 1580 1590 1600
AEASLEHEEG KILRIQLELN QVKSEIDRKI AEKDEELDQL KRNHLRVVES
1610 1620 1630 1640 1650
MQSTLDAEIR SRNDALRIKK KMEGDLNEME IQLNHANRQA AEALRNLRNT
1660 1670 1680 1690 1700
QGILKDTQLH LDDAIRGQDD LKEQLAMVER RANLMQAEVE ELRASLERTE
1710 1720 1730 1740 1750
RGRKMAEQEL LDASERVQLL HTQNTSLINT KKKLETDISQ IQGEMEDIVQ
1760 1770 1780 1790 1800
EARNAEEKAK KAITDAAMMA EELKKEQDTS AHLERMKKNM EQTVKDLQLR
1810 1820 1830 1840 1850
LDEAEQLALK GGKKQIQKLE ARVRELESEV ESEQKHNVEA VKGLRKHERR
1860 1870 1880 1890 1900
VKELTYQTEE DRKNILRLQD LVDKLQTKVK AYKRQAEEAE EQSNVNLAKF
1910 1920 1930
RKLQHELEEA KERADIAESQ VNKLRVKSRE VHTKVISEE
Length:1,939
Mass (Da):223,071
Last modified:March 3, 2009 - v2
Checksum:i023D6F73C476B19B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1167 – 11671M → L in AAD29949 (PubMed:10388558).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti594 – 5941A → T.
Corresponds to variant rs12949680 [ dbSNP | Ensembl ].
VAR_030198
Natural varianti883 – 8831T → M.
Corresponds to variant rs3744558 [ dbSNP | Ensembl ].
VAR_022110
Natural varianti1106 – 11061I → M.1 Publication
Corresponds to variant rs917361 [ dbSNP | Ensembl ].
VAR_030199
Natural varianti1117 – 11171A → D.
Corresponds to variant rs16943441 [ dbSNP | Ensembl ].
VAR_030200
Natural varianti1209 – 12091E → K.1 Publication
Corresponds to variant rs11651295 [ dbSNP | Ensembl ].
VAR_030201
Natural varianti1802 – 18021D → G.1 Publication
Corresponds to variant rs2277649 [ dbSNP | Ensembl ].
VAR_030202
Natural varianti1862 – 18621R → C.
Corresponds to variant rs34260986 [ dbSNP | Ensembl ].
VAR_056175
Natural varianti1911 – 19111K → E.1 Publication
Corresponds to variant rs3744554 [ dbSNP | Ensembl ].
VAR_024542

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF111783 mRNA. Translation: AAD29949.1.
AC005323 Genomic DNA. No translation available.
CCDSiCCDS11154.1.
RefSeqiNP_060003.2. NM_017533.2.
UniGeneiHs.711141.

Genome annotation databases

EnsembliENST00000255381; ENSP00000255381; ENSG00000264424.
GeneIDi4622.
KEGGihsa:4622.
UCSCiuc002gmn.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF111783 mRNA. Translation: AAD29949.1.
AC005323 Genomic DNA. No translation available.
CCDSiCCDS11154.1.
RefSeqiNP_060003.2. NM_017533.2.
UniGeneiHs.711141.

3D structure databases

ProteinModelPortaliQ9Y623.
SMRiQ9Y623. Positions 4-967.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110707. 11 interactions.
IntActiQ9Y623. 4 interactions.
MINTiMINT-5003666.
STRINGi9606.ENSP00000255381.

PTM databases

PhosphoSiteiQ9Y623.

Polymorphism and mutation databases

BioMutaiMYH4.
DMDMi224471840.

2D gel databases

UCD-2DPAGEQ9Y623.

Proteomic databases

MaxQBiQ9Y623.
PaxDbiQ9Y623.
PRIDEiQ9Y623.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000255381; ENSP00000255381; ENSG00000264424.
GeneIDi4622.
KEGGihsa:4622.
UCSCiuc002gmn.3. human.

Organism-specific databases

CTDi4622.
GeneCardsiGC17M010346.
H-InvDBHIX0039061.
HGNCiHGNC:7574. MYH4.
HPAiHPA001349.
MIMi160742. gene.
neXtProtiNX_Q9Y623.
PharmGKBiPA31371.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5022.
GeneTreeiENSGT00760000118919.
HOGENOMiHOG000173959.
HOVERGENiHBG004704.
InParanoidiQ9Y623.
KOiK10352.
OMAiDDQVFSM.
OrthoDBiEOG7RBZ7G.
PhylomeDBiQ9Y623.
TreeFamiTF314375.

Enzyme and pathway databases

ReactomeiREACT_147867. Translocation of GLUT4 to the plasma membrane.

Miscellaneous databases

GeneWikiiMYH4.
GenomeRNAii4622.
NextBioi17794.
PROiQ9Y623.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Y623.
CleanExiHS_MYH4.
GenevisibleiQ9Y623. HS.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 2 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Comparative sequence analysis of the complete human sarcomeric myosin heavy chain family: implications for functional diversity."
    Weiss A., Schiaffino S., Leinwand L.A.
    J. Mol. Biol. 290:61-75(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS MET-1106; LYS-1209; GLY-1802 AND GLU-1911.
    Tissue: Skeletal muscle.
  2. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry."
    Denis N.J., Vasilescu J., Lambert J.-P., Smith J.C., Figeys D.
    Proteomics 7:868-874(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-1813.
    Tissue: Mammary cancer.

Entry informationi

Entry nameiMYH4_HUMAN
AccessioniPrimary (citable) accession number: Q9Y623
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 3, 2009
Last modified: July 22, 2015
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Represents a conventional myosin. This protein should not be confused with the unconventional myosin-4 (MYO4).Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.