Q9Y623 (MYH4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myosin-4

Alternative name(s):
Myosin heavy chain 2b
Short name=MyHC-2b
Myosin heavy chain 4
Myosin heavy chain IIb
Short name=MyHC-IIb
Myosin heavy chain, skeletal muscle, fetal

Gene names

Name:

MYH4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1939 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Muscle contraction.
Subunit structure	Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).
Subcellular location	Cytoplasm › myofibril. Note: Thick filaments of the myofibrils.
Domain	The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils. Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
Sequence similarities	Contains 1 IQ domain. Contains 1 myosin head-like domain.
Caution	Represents a conventional myosin. This protein should not be confused with the unconventional myosin-4 (MYO4).

Ontologies

Keywords
Cellular component	Cytoplasm Thick filament
Coding sequence diversity	Polymorphism
Domain	Coiled coil
Ligand	ATP-binding Actin-binding Calmodulin-binding Nucleotide-binding
Molecular function	Motor protein Muscle protein Myosin
PTM	Isopeptide bond Methylation Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	muscle filament sliding Non-traceable author statement PubMed 3904738. Source: BHF-UCL response to activity Inferred from electronic annotation. Source: Compara
Cellular_component	focal adhesion Inferred from direct assay. Source: HPA muscle myosin complex Non-traceable author statement PubMed 3904738. Source: BHF-UCL myosin filament Inferred from electronic annotation. Source: UniProtKB-KW nucleolus Inferred from direct assay. Source: HPA sarcomere Non-traceable author statement PubMed 3904738. Source: BHF-UCL
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW microfilament motor activity Non-traceable author statement Ref.1. Source: UniProtKB structural constituent of muscle Non-traceable author statement PubMed 3904738. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1939	1939	Myosin-4		PRO_0000123398
Regions
	Domain	1 – 784	784	Myosin head-like
	Domain	785 – 814	30	IQ
	Nucleotide binding	179 – 186	8	ATP Potential
	Region	659 – 681	23	Actin-binding By similarity
	Region	761 – 775	15	Actin-binding By similarity
	Coiled coil	843 – 1939	1097	Potential
Amino acid modifications
	Modified residue	130	1	N6,N6,N6-trimethyllysine Potential
	Cross-link	1813		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.3
Natural variations
	Natural variant	594	1	A → T. Corresponds to variant rs12949680 [ dbSNP | Ensembl ].		VAR_030198
	Natural variant	883	1	T → M. Corresponds to variant rs3744558 [ dbSNP | Ensembl ].		VAR_022110
	Natural variant	1106	1	I → M. Ref.1 Corresponds to variant rs917361 [ dbSNP | Ensembl ].		VAR_030199
	Natural variant	1117	1	A → D. Corresponds to variant rs16943441 [ dbSNP | Ensembl ].		VAR_030200
	Natural variant	1209	1	E → K. Ref.1 Corresponds to variant rs11651295 [ dbSNP | Ensembl ].		VAR_030201
	Natural variant	1802	1	D → G. Ref.1 Corresponds to variant rs2277649 [ dbSNP | Ensembl ].		VAR_030202
	Natural variant	1862	1	R → C. Corresponds to variant rs34260986 [ dbSNP | Ensembl ].		VAR_056175
	Natural variant	1911	1	K → E. Ref.1 Corresponds to variant rs3744554 [ dbSNP | Ensembl ].		VAR_024542
Experimental info
	Sequence conflict	1167	1	M → L in AAD29949. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9Y623 [UniParc]. Last modified March 3, 2009. Version 2. Checksum: 023D6F73C476B19B	FASTA	1,939	223,071
	10 20 30 40 50 60 MSSDSEMAIF GEAAPFLRKS EKERIEAQNK PFDAKTSVFV VDPKESYVKA IVQSREGGKV 70 80 90 100 110 120 TAKTEAGATV TVKEDQVFSM NPPKYDKIED MAMMTHLHEP AVLYNLKERY AAWMIYTYSG 130 140 150 160 170 180 LFCVTVNPYK WLPVYNPEVV TAYRGKKRQE APPHIFSISD NAYQFMLTDR ENQSILITGE 190 200 210 220 230 240 SGAGKTVNTK RVIQYFATIA VTGEKKKEEP ASGKMQGTLE DQIISANPLL EAFGNAKTVR 250 260 270 280 290 300 NDNSSRFGKF IRIHFGATGK LASADIETYL LEKSRVTFQL KAERSYHIFY QILSNKKPEL 310 320 330 340 350 360 IEMLLITTNP YDFAFVSQGE ITVPSIDDQE ELMATDSAVD ILGFTADEKV AIYKLTGAVM 370 380 390 400 410 420 HYGNMKFKQK QREEQAEPDG TEVADKAAYL TSLNSADLLK SLCYPRVKVG NEFVTKGQTV 430 440 450 460 470 480 QQVYNAVGAL AKAIYEKMFL WMVTRINQQL DTKQPRQYFI GVLDIAGFEI FDFNSLEQLC 490 500 510 520 530 540 INFTNEKLQQ FFNHHMFVLE QEEYKKEGIE WEFIDFGMDL AACIELIEKP MGIFSILEEE 550 560 570 580 590 600 CMFPKATDTS FKNKLYEQHL GKSNNFQKPK PAKGKPEAHF SLVHYAGTVD YNIAGWLDKN 610 620 630 640 650 660 KDPLNETVVG LYQKSAMKTL AFLFSGAQTA EAEGGGGKKG GKKKGSSFQT VSALFRENLN 670 680 690 700 710 720 KLMTNLRSTH PHFVRCIIPN ETKTPGAMEH ELVLHQLRCN GVLEGIRICR KGFPSRILYA 730 740 750 760 770 780 DFKQRYKVLN ASAIPEGQFI DSKKASEKLL GSIEIDHTQY KFGHTKVFFK AGLLGTLEEM 790 800 810 820 830 840 RDEKLAQLIT RTQAICRGFL MRVEFRKMME RRESIFCIQY NIRAFMNVKH WPWMKLYFKI 850 860 870 880 890 900 KPLLKSAETE KEMANMKEEF EKTKEELAKT EAKRKELEEK MVTLMQEKND LQLQVQAEAD 910 920 930 940 950 960 ALADAEERCD QLIKTKIQLE AKIKEVTERA EDEEEINAEL TAKKRKLEDE CSELKKDIDD 970 980 990 1000 1010 1020 LELTLAKVEK EKHATENKVK NLTEEMAGLD ETIAKLTKEK KALQEAHQQT LDDLQMEEDK 1030 1040 1050 1060 1070 1080 VNTLTKAKTK LEQQVDDLEG SLEQEKKLCM DLERAKRKLE GDLKLAQEST MDTENDKQQL 1090 1100 1110 1120 1130 1140 NEKLKKKEFE MSNLQGKIED EQALAIQLQK KIKELQARIE ELEEEIEAER ASRAKAEKQR 1150 1160 1170 1180 1190 1200 SDLSRELEEI SERLEEAGGA TSAQIEMNKK REAEFQKMRR DLEESTLQHE ATAAALRKKH 1210 1220 1230 1240 1250 1260 ADSVAELGEQ IDSLQRVKQK LEKEKSELKM EINDLASNME TVSKAKANFE KMCRTLEDQL 1270 1280 1290 1300 1310 1320 SEIKTKEEEQ QRLINELSAQ KARLHTESGE FSRQLDEKDA MVSQLSRGKQ AFTQQIEELK 1330 1340 1350 1360 1370 1380 RQLEEETKAK STLAHALQSA RHDCDLLREQ YEEEQEAKAE LQRGMSKANS EVAQWRTKYE 1390 1400 1410 1420 1430 1440 TDAIQRTEEL EEAKKKLAQR LQDAEEHVEA VNSKCASLEK TKQRLQNEVE DLMIDVERSN 1450 1460 1470 1480 1490 1500 AACIALDKKQ RNFDKVLAEW KQKYEETQAE LEASQKESRS LSTELFKVKN AYEESLDHLE 1510 1520 1530 1540 1550 1560 TLKRENKNLQ QEISDLTEQI AEGGKHIHEL EKVKKQLDHE KSELQTSLEE AEASLEHEEG 1570 1580 1590 1600 1610 1620 KILRIQLELN QVKSEIDRKI AEKDEELDQL KRNHLRVVES MQSTLDAEIR SRNDALRIKK 1630 1640 1650 1660 1670 1680 KMEGDLNEME IQLNHANRQA AEALRNLRNT QGILKDTQLH LDDAIRGQDD LKEQLAMVER 1690 1700 1710 1720 1730 1740 RANLMQAEVE ELRASLERTE RGRKMAEQEL LDASERVQLL HTQNTSLINT KKKLETDISQ 1750 1760 1770 1780 1790 1800 IQGEMEDIVQ EARNAEEKAK KAITDAAMMA EELKKEQDTS AHLERMKKNM EQTVKDLQLR 1810 1820 1830 1840 1850 1860 LDEAEQLALK GGKKQIQKLE ARVRELESEV ESEQKHNVEA VKGLRKHERR VKELTYQTEE 1870 1880 1890 1900 1910 1920 DRKNILRLQD LVDKLQTKVK AYKRQAEEAE EQSNVNLAKF RKLQHELEEA KERADIAESQ 1930 VNKLRVKSRE VHTKVISEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Comparative sequence analysis of the complete human sarcomeric myosin heavy chain family: implications for functional diversity." Weiss A., Schiaffino S., Leinwand L.A. J. Mol. Biol. 290:61-75(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS MET-1106; LYS-1209; GLY-1802 AND GLU-1911. Tissue: Skeletal muscle.
[2]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry." Denis N.J., Vasilescu J., Lambert J.-P., Smith J.C., Figeys D. Proteomics 7:868-874(2007) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-1813, MASS SPECTROMETRY. Tissue: Mammary cancer.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF111783 mRNA. Translation: AAD29949.1. AC005323 Genomic DNA. No translation available.
IPI	IPI00001753.
RefSeq	NP_060003.2. NM_017533.2.
UniGene	Hs.711141.
3D structure databases
ProteinModelPortal	Q9Y623.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y623. 3 interactions.
STRING	9606.ENSP00000255381.
PTM databases
PhosphoSite	Q9Y623.
Polymorphism databases
DMDM	224471840.
2D gel databases
UCD-2DPAGE	Q9Y623.
Proteomic databases
PaxDb	Q9Y623.
PRIDE	Q9Y623.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000255381; ENSP00000255381; ENSG00000264424.
GeneID	4622.
KEGG	hsa:4622.
UCSC	uc002gmn.3. human.
Organism-specific databases
CTD	4622.
GeneCards	GC17M010346.
H-InvDB	HIX0039061.
HGNC	HGNC:7574. MYH4.
HPA	HPA001239. HPA001349.
MIM	160742. gene.
neXtProt	NX_Q9Y623.
PharmGKB	PA31371.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5022.
HOGENOM	HOG000173959.
HOVERGEN	HBG004704.
InParanoid	Q9Y623.
KO	K10352.
OMA	NTQGMLK.
OrthoDB	EOG43N7BR.
PhylomeDB	Q9Y623.
Enzyme and pathway databases
Reactome	REACT_11123. Membrane Trafficking.
Gene expression databases
Bgee	Q9Y623.
CleanEx	HS_MYH4.
Genevestigator	Q9Y623.
GermOnline	ENSG00000141048. Homo sapiens.
Family and domain databases
InterPro	IPR000048. IQ_motif_EF-hand-BS. IPR015650. Myosin_1/23/4/7/8/13/15. IPR001609. Myosin_head_motor_dom. IPR004009. Myosin_N. IPR002928. Myosin_tail. [Graphical view]
PANTHER	PTHR13140:SF22. PTHR13140:SF22. 1 hit.
Pfam	PF00063. Myosin_head. 1 hit. PF02736. Myosin_N. 1 hit. PF01576. Myosin_tail_1. 1 hit. [Graphical view]
PRINTS	PR00193. MYOSINHEAVY.
SMART	SM00015. IQ. 2 hits. SM00242. MYSc. 1 hit. [Graphical view]
PROSITE	PS50096. IQ. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	4622.
NextBio	17794.
SOURCE	Search...

Entry information

Entry name

MYH4_HUMAN

Accession

Primary (citable) accession number: Q9Y623

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.