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Q9Y623

- MYH4_HUMAN

UniProt

Q9Y623 - MYH4_HUMAN

Protein

Myosin-4

Gene

MYH4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Muscle contraction.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi179 – 1868ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. double-stranded RNA binding Source: MGI
    3. microfilament motor activity Source: UniProtKB

    GO - Biological processi

    1. actin filament-based movement Source: UniProtKB
    2. ATP catabolic process Source: BHF-UCL
    3. membrane organization Source: Reactome
    4. muscle contraction Source: BHF-UCL
    5. muscle filament sliding Source: BHF-UCL
    6. response to activity Source: Ensembl

    Keywords - Molecular functioni

    Motor protein, Muscle protein, Myosin

    Keywords - Ligandi

    Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_147867. Translocation of GLUT4 to the plasma membrane.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myosin-4
    Alternative name(s):
    Myosin heavy chain 2b
    Short name:
    MyHC-2b
    Myosin heavy chain 4
    Myosin heavy chain IIb
    Short name:
    MyHC-IIb
    Myosin heavy chain, skeletal muscle, fetal
    Gene namesi
    Name:MYH4
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:7574. MYH4.

    Subcellular locationi

    Cytoplasmmyofibril
    Note: Thick filaments of the myofibrils.

    GO - Cellular componenti

    1. muscle myosin complex Source: BHF-UCL
    2. myofibril Source: BHF-UCL
    3. myosin filament Source: UniProtKB-KW
    4. sarcomere Source: BHF-UCL

    Keywords - Cellular componenti

    Cytoplasm, Thick filament

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA31371.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 19391939Myosin-4PRO_0000123398Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei130 – 1301N6,N6,N6-trimethyllysineSequence Analysis
    Cross-linki1813 – 1813Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

    Keywords - PTMi

    Isopeptide bond, Methylation, Ubl conjugation

    Proteomic databases

    MaxQBiQ9Y623.
    PaxDbiQ9Y623.
    PRIDEiQ9Y623.

    2D gel databases

    UCD-2DPAGEQ9Y623.

    PTM databases

    PhosphoSiteiQ9Y623.

    Expressioni

    Gene expression databases

    BgeeiQ9Y623.
    CleanExiHS_MYH4.
    GenevestigatoriQ9Y623.

    Organism-specific databases

    HPAiHPA001239.
    HPA001349.

    Interactioni

    Subunit structurei

    Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

    Protein-protein interaction databases

    BioGridi110707. 5 interactions.
    IntActiQ9Y623. 3 interactions.
    MINTiMINT-5003666.
    STRINGi9606.ENSP00000255381.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Y623.
    SMRiQ9Y623. Positions 4-967.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini86 – 782697Myosin motorAdd
    BLAST
    Domaini785 – 81430IQPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni659 – 68123Actin-bindingBy similarityAdd
    BLAST
    Regioni761 – 77515Actin-bindingBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili843 – 19391097Sequence AnalysisAdd
    BLAST

    Domaini

    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

    Sequence similaritiesi

    Contains 1 IQ domain.PROSITE-ProRule annotation
    Contains 1 myosin motor domain.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5022.
    HOGENOMiHOG000173959.
    HOVERGENiHBG004704.
    InParanoidiQ9Y623.
    KOiK10352.
    OMAiNTQGMLK.
    OrthoDBiEOG7RBZ7G.
    PhylomeDBiQ9Y623.
    TreeFamiTF314375.

    Family and domain databases

    Gene3Di4.10.270.10. 1 hit.
    InterProiIPR000048. IQ_motif_EF-hand-BS.
    IPR027401. Myosin-like_IQ_dom.
    IPR001609. Myosin_head_motor_dom.
    IPR004009. Myosin_N.
    IPR002928. Myosin_tail.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00063. Myosin_head. 1 hit.
    PF02736. Myosin_N. 1 hit.
    PF01576. Myosin_tail_1. 1 hit.
    [Graphical view]
    PRINTSiPR00193. MYOSINHEAVY.
    SMARTiSM00015. IQ. 2 hits.
    SM00242. MYSc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS50096. IQ. 1 hit.
    PS51456. MYOSIN_MOTOR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Y623-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSDSEMAIF GEAAPFLRKS EKERIEAQNK PFDAKTSVFV VDPKESYVKA     50
    IVQSREGGKV TAKTEAGATV TVKEDQVFSM NPPKYDKIED MAMMTHLHEP 100
    AVLYNLKERY AAWMIYTYSG LFCVTVNPYK WLPVYNPEVV TAYRGKKRQE 150
    APPHIFSISD NAYQFMLTDR ENQSILITGE SGAGKTVNTK RVIQYFATIA 200
    VTGEKKKEEP ASGKMQGTLE DQIISANPLL EAFGNAKTVR NDNSSRFGKF 250
    IRIHFGATGK LASADIETYL LEKSRVTFQL KAERSYHIFY QILSNKKPEL 300
    IEMLLITTNP YDFAFVSQGE ITVPSIDDQE ELMATDSAVD ILGFTADEKV 350
    AIYKLTGAVM HYGNMKFKQK QREEQAEPDG TEVADKAAYL TSLNSADLLK 400
    SLCYPRVKVG NEFVTKGQTV QQVYNAVGAL AKAIYEKMFL WMVTRINQQL 450
    DTKQPRQYFI GVLDIAGFEI FDFNSLEQLC INFTNEKLQQ FFNHHMFVLE 500
    QEEYKKEGIE WEFIDFGMDL AACIELIEKP MGIFSILEEE CMFPKATDTS 550
    FKNKLYEQHL GKSNNFQKPK PAKGKPEAHF SLVHYAGTVD YNIAGWLDKN 600
    KDPLNETVVG LYQKSAMKTL AFLFSGAQTA EAEGGGGKKG GKKKGSSFQT 650
    VSALFRENLN KLMTNLRSTH PHFVRCIIPN ETKTPGAMEH ELVLHQLRCN 700
    GVLEGIRICR KGFPSRILYA DFKQRYKVLN ASAIPEGQFI DSKKASEKLL 750
    GSIEIDHTQY KFGHTKVFFK AGLLGTLEEM RDEKLAQLIT RTQAICRGFL 800
    MRVEFRKMME RRESIFCIQY NIRAFMNVKH WPWMKLYFKI KPLLKSAETE 850
    KEMANMKEEF EKTKEELAKT EAKRKELEEK MVTLMQEKND LQLQVQAEAD 900
    ALADAEERCD QLIKTKIQLE AKIKEVTERA EDEEEINAEL TAKKRKLEDE 950
    CSELKKDIDD LELTLAKVEK EKHATENKVK NLTEEMAGLD ETIAKLTKEK 1000
    KALQEAHQQT LDDLQMEEDK VNTLTKAKTK LEQQVDDLEG SLEQEKKLCM 1050
    DLERAKRKLE GDLKLAQEST MDTENDKQQL NEKLKKKEFE MSNLQGKIED 1100
    EQALAIQLQK KIKELQARIE ELEEEIEAER ASRAKAEKQR SDLSRELEEI 1150
    SERLEEAGGA TSAQIEMNKK REAEFQKMRR DLEESTLQHE ATAAALRKKH 1200
    ADSVAELGEQ IDSLQRVKQK LEKEKSELKM EINDLASNME TVSKAKANFE 1250
    KMCRTLEDQL SEIKTKEEEQ QRLINELSAQ KARLHTESGE FSRQLDEKDA 1300
    MVSQLSRGKQ AFTQQIEELK RQLEEETKAK STLAHALQSA RHDCDLLREQ 1350
    YEEEQEAKAE LQRGMSKANS EVAQWRTKYE TDAIQRTEEL EEAKKKLAQR 1400
    LQDAEEHVEA VNSKCASLEK TKQRLQNEVE DLMIDVERSN AACIALDKKQ 1450
    RNFDKVLAEW KQKYEETQAE LEASQKESRS LSTELFKVKN AYEESLDHLE 1500
    TLKRENKNLQ QEISDLTEQI AEGGKHIHEL EKVKKQLDHE KSELQTSLEE 1550
    AEASLEHEEG KILRIQLELN QVKSEIDRKI AEKDEELDQL KRNHLRVVES 1600
    MQSTLDAEIR SRNDALRIKK KMEGDLNEME IQLNHANRQA AEALRNLRNT 1650
    QGILKDTQLH LDDAIRGQDD LKEQLAMVER RANLMQAEVE ELRASLERTE 1700
    RGRKMAEQEL LDASERVQLL HTQNTSLINT KKKLETDISQ IQGEMEDIVQ 1750
    EARNAEEKAK KAITDAAMMA EELKKEQDTS AHLERMKKNM EQTVKDLQLR 1800
    LDEAEQLALK GGKKQIQKLE ARVRELESEV ESEQKHNVEA VKGLRKHERR 1850
    VKELTYQTEE DRKNILRLQD LVDKLQTKVK AYKRQAEEAE EQSNVNLAKF 1900
    RKLQHELEEA KERADIAESQ VNKLRVKSRE VHTKVISEE 1939
    Length:1,939
    Mass (Da):223,071
    Last modified:March 3, 2009 - v2
    Checksum:i023D6F73C476B19B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1167 – 11671M → L in AAD29949. (PubMed:10388558)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti594 – 5941A → T.
    Corresponds to variant rs12949680 [ dbSNP | Ensembl ].
    VAR_030198
    Natural varianti883 – 8831T → M.
    Corresponds to variant rs3744558 [ dbSNP | Ensembl ].
    VAR_022110
    Natural varianti1106 – 11061I → M.1 Publication
    Corresponds to variant rs917361 [ dbSNP | Ensembl ].
    VAR_030199
    Natural varianti1117 – 11171A → D.
    Corresponds to variant rs16943441 [ dbSNP | Ensembl ].
    VAR_030200
    Natural varianti1209 – 12091E → K.1 Publication
    Corresponds to variant rs11651295 [ dbSNP | Ensembl ].
    VAR_030201
    Natural varianti1802 – 18021D → G.1 Publication
    Corresponds to variant rs2277649 [ dbSNP | Ensembl ].
    VAR_030202
    Natural varianti1862 – 18621R → C.
    Corresponds to variant rs34260986 [ dbSNP | Ensembl ].
    VAR_056175
    Natural varianti1911 – 19111K → E.1 Publication
    Corresponds to variant rs3744554 [ dbSNP | Ensembl ].
    VAR_024542

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF111783 mRNA. Translation: AAD29949.1.
    AC005323 Genomic DNA. No translation available.
    CCDSiCCDS11154.1.
    RefSeqiNP_060003.2. NM_017533.2.
    UniGeneiHs.711141.

    Genome annotation databases

    EnsembliENST00000255381; ENSP00000255381; ENSG00000264424.
    GeneIDi4622.
    KEGGihsa:4622.
    UCSCiuc002gmn.3. human.

    Polymorphism databases

    DMDMi224471840.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF111783 mRNA. Translation: AAD29949.1 .
    AC005323 Genomic DNA. No translation available.
    CCDSi CCDS11154.1.
    RefSeqi NP_060003.2. NM_017533.2.
    UniGenei Hs.711141.

    3D structure databases

    ProteinModelPortali Q9Y623.
    SMRi Q9Y623. Positions 4-967.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110707. 5 interactions.
    IntActi Q9Y623. 3 interactions.
    MINTi MINT-5003666.
    STRINGi 9606.ENSP00000255381.

    PTM databases

    PhosphoSitei Q9Y623.

    Polymorphism databases

    DMDMi 224471840.

    2D gel databases

    UCD-2DPAGE Q9Y623.

    Proteomic databases

    MaxQBi Q9Y623.
    PaxDbi Q9Y623.
    PRIDEi Q9Y623.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000255381 ; ENSP00000255381 ; ENSG00000264424 .
    GeneIDi 4622.
    KEGGi hsa:4622.
    UCSCi uc002gmn.3. human.

    Organism-specific databases

    CTDi 4622.
    GeneCardsi GC17M010346.
    H-InvDB HIX0039061.
    HGNCi HGNC:7574. MYH4.
    HPAi HPA001239.
    HPA001349.
    MIMi 160742. gene.
    neXtProti NX_Q9Y623.
    PharmGKBi PA31371.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5022.
    HOGENOMi HOG000173959.
    HOVERGENi HBG004704.
    InParanoidi Q9Y623.
    KOi K10352.
    OMAi NTQGMLK.
    OrthoDBi EOG7RBZ7G.
    PhylomeDBi Q9Y623.
    TreeFami TF314375.

    Enzyme and pathway databases

    Reactomei REACT_147867. Translocation of GLUT4 to the plasma membrane.

    Miscellaneous databases

    GeneWikii MYH4.
    GenomeRNAii 4622.
    NextBioi 17794.
    PROi Q9Y623.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9Y623.
    CleanExi HS_MYH4.
    Genevestigatori Q9Y623.

    Family and domain databases

    Gene3Di 4.10.270.10. 1 hit.
    InterProi IPR000048. IQ_motif_EF-hand-BS.
    IPR027401. Myosin-like_IQ_dom.
    IPR001609. Myosin_head_motor_dom.
    IPR004009. Myosin_N.
    IPR002928. Myosin_tail.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00063. Myosin_head. 1 hit.
    PF02736. Myosin_N. 1 hit.
    PF01576. Myosin_tail_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00193. MYOSINHEAVY.
    SMARTi SM00015. IQ. 2 hits.
    SM00242. MYSc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS50096. IQ. 1 hit.
    PS51456. MYOSIN_MOTOR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative sequence analysis of the complete human sarcomeric myosin heavy chain family: implications for functional diversity."
      Weiss A., Schiaffino S., Leinwand L.A.
      J. Mol. Biol. 290:61-75(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS MET-1106; LYS-1209; GLY-1802 AND GLU-1911.
      Tissue: Skeletal muscle.
    2. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
      Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
      , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
      Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry."
      Denis N.J., Vasilescu J., Lambert J.-P., Smith J.C., Figeys D.
      Proteomics 7:868-874(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-1813.
      Tissue: Mammary cancer.

    Entry informationi

    Entry nameiMYH4_HUMAN
    AccessioniPrimary (citable) accession number: Q9Y623
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    Represents a conventional myosin. This protein should not be confused with the unconventional myosin-4 (MYO4).Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3