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Q9Y623 (MYH4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myosin-4
Alternative name(s):
Myosin heavy chain 2b
Short name=MyHC-2b
Myosin heavy chain 4
Myosin heavy chain IIb
Short name=MyHC-IIb
Myosin heavy chain, skeletal muscle, fetal
Gene names
Name:MYH4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1939 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Muscle contraction.

Subunit structure

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Subcellular location

Cytoplasmmyofibril. Note: Thick filaments of the myofibrils.

Domain

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Sequence similarities

Contains 1 IQ domain.

Contains 1 myosin head-like domain.

Caution

Represents a conventional myosin. This protein should not be confused with the unconventional myosin-4 (MYO4).

Ontologies

Keywords
   Cellular componentCytoplasm
Thick filament
   Coding sequence diversityPolymorphism
   DomainCoiled coil
   LigandActin-binding
ATP-binding
Calmodulin-binding
Nucleotide-binding
   Molecular functionMotor protein
Muscle protein
Myosin
   PTMIsopeptide bond
Methylation
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactin filament-based movement

Non-traceable author statement Ref.1. Source: UniProtKB

membrane organization

Traceable author statement. Source: Reactome

metabolic process

Non-traceable author statement. Source: GOC

muscle contraction

Inferred from direct assay PubMed 8145163. Source: BHF-UCL

muscle filament sliding

Non-traceable author statement PubMed 3904738. Source: BHF-UCL

response to activity

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentmuscle myosin complex

Non-traceable author statement PubMed 3904738. Source: BHF-UCL

myofibril

Inferred from direct assay PubMed 8145163. Source: BHF-UCL

myosin filament

Inferred from electronic annotation. Source: UniProtKB-KW

sarcomere

Non-traceable author statement PubMed 3904738. Source: BHF-UCL

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

double-stranded RNA binding

Inferred from direct assay PubMed 21266579. Source: MGI

microfilament motor activity

Non-traceable author statement Ref.1. Source: UniProtKB

structural constituent of muscle

Non-traceable author statement. Source: BHF-UCL

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19391939Myosin-4
PRO_0000123398

Regions

Domain1 – 784784Myosin head-like
Domain785 – 81430IQ
Nucleotide binding179 – 1868ATP Potential
Region659 – 68123Actin-binding By similarity
Region761 – 77515Actin-binding By similarity
Coiled coil843 – 19391097 Potential

Amino acid modifications

Modified residue1301N6,N6,N6-trimethyllysine Potential
Cross-link1813Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.3

Natural variations

Natural variant5941A → T.
Corresponds to variant rs12949680 [ dbSNP | Ensembl ].
VAR_030198
Natural variant8831T → M.
Corresponds to variant rs3744558 [ dbSNP | Ensembl ].
VAR_022110
Natural variant11061I → M. Ref.1
Corresponds to variant rs917361 [ dbSNP | Ensembl ].
VAR_030199
Natural variant11171A → D.
Corresponds to variant rs16943441 [ dbSNP | Ensembl ].
VAR_030200
Natural variant12091E → K. Ref.1
Corresponds to variant rs11651295 [ dbSNP | Ensembl ].
VAR_030201
Natural variant18021D → G. Ref.1
Corresponds to variant rs2277649 [ dbSNP | Ensembl ].
VAR_030202
Natural variant18621R → C.
Corresponds to variant rs34260986 [ dbSNP | Ensembl ].
VAR_056175
Natural variant19111K → E. Ref.1
Corresponds to variant rs3744554 [ dbSNP | Ensembl ].
VAR_024542

Experimental info

Sequence conflict11671M → L in AAD29949. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9Y623 [UniParc].

Last modified March 3, 2009. Version 2.
Checksum: 023D6F73C476B19B

FASTA1,939223,071
        10         20         30         40         50         60 
MSSDSEMAIF GEAAPFLRKS EKERIEAQNK PFDAKTSVFV VDPKESYVKA IVQSREGGKV 

        70         80         90        100        110        120 
TAKTEAGATV TVKEDQVFSM NPPKYDKIED MAMMTHLHEP AVLYNLKERY AAWMIYTYSG 

       130        140        150        160        170        180 
LFCVTVNPYK WLPVYNPEVV TAYRGKKRQE APPHIFSISD NAYQFMLTDR ENQSILITGE 

       190        200        210        220        230        240 
SGAGKTVNTK RVIQYFATIA VTGEKKKEEP ASGKMQGTLE DQIISANPLL EAFGNAKTVR 

       250        260        270        280        290        300 
NDNSSRFGKF IRIHFGATGK LASADIETYL LEKSRVTFQL KAERSYHIFY QILSNKKPEL 

       310        320        330        340        350        360 
IEMLLITTNP YDFAFVSQGE ITVPSIDDQE ELMATDSAVD ILGFTADEKV AIYKLTGAVM 

       370        380        390        400        410        420 
HYGNMKFKQK QREEQAEPDG TEVADKAAYL TSLNSADLLK SLCYPRVKVG NEFVTKGQTV 

       430        440        450        460        470        480 
QQVYNAVGAL AKAIYEKMFL WMVTRINQQL DTKQPRQYFI GVLDIAGFEI FDFNSLEQLC 

       490        500        510        520        530        540 
INFTNEKLQQ FFNHHMFVLE QEEYKKEGIE WEFIDFGMDL AACIELIEKP MGIFSILEEE 

       550        560        570        580        590        600 
CMFPKATDTS FKNKLYEQHL GKSNNFQKPK PAKGKPEAHF SLVHYAGTVD YNIAGWLDKN 

       610        620        630        640        650        660 
KDPLNETVVG LYQKSAMKTL AFLFSGAQTA EAEGGGGKKG GKKKGSSFQT VSALFRENLN 

       670        680        690        700        710        720 
KLMTNLRSTH PHFVRCIIPN ETKTPGAMEH ELVLHQLRCN GVLEGIRICR KGFPSRILYA 

       730        740        750        760        770        780 
DFKQRYKVLN ASAIPEGQFI DSKKASEKLL GSIEIDHTQY KFGHTKVFFK AGLLGTLEEM 

       790        800        810        820        830        840 
RDEKLAQLIT RTQAICRGFL MRVEFRKMME RRESIFCIQY NIRAFMNVKH WPWMKLYFKI 

       850        860        870        880        890        900 
KPLLKSAETE KEMANMKEEF EKTKEELAKT EAKRKELEEK MVTLMQEKND LQLQVQAEAD 

       910        920        930        940        950        960 
ALADAEERCD QLIKTKIQLE AKIKEVTERA EDEEEINAEL TAKKRKLEDE CSELKKDIDD 

       970        980        990       1000       1010       1020 
LELTLAKVEK EKHATENKVK NLTEEMAGLD ETIAKLTKEK KALQEAHQQT LDDLQMEEDK 

      1030       1040       1050       1060       1070       1080 
VNTLTKAKTK LEQQVDDLEG SLEQEKKLCM DLERAKRKLE GDLKLAQEST MDTENDKQQL 

      1090       1100       1110       1120       1130       1140 
NEKLKKKEFE MSNLQGKIED EQALAIQLQK KIKELQARIE ELEEEIEAER ASRAKAEKQR 

      1150       1160       1170       1180       1190       1200 
SDLSRELEEI SERLEEAGGA TSAQIEMNKK REAEFQKMRR DLEESTLQHE ATAAALRKKH 

      1210       1220       1230       1240       1250       1260 
ADSVAELGEQ IDSLQRVKQK LEKEKSELKM EINDLASNME TVSKAKANFE KMCRTLEDQL 

      1270       1280       1290       1300       1310       1320 
SEIKTKEEEQ QRLINELSAQ KARLHTESGE FSRQLDEKDA MVSQLSRGKQ AFTQQIEELK 

      1330       1340       1350       1360       1370       1380 
RQLEEETKAK STLAHALQSA RHDCDLLREQ YEEEQEAKAE LQRGMSKANS EVAQWRTKYE 

      1390       1400       1410       1420       1430       1440 
TDAIQRTEEL EEAKKKLAQR LQDAEEHVEA VNSKCASLEK TKQRLQNEVE DLMIDVERSN 

      1450       1460       1470       1480       1490       1500 
AACIALDKKQ RNFDKVLAEW KQKYEETQAE LEASQKESRS LSTELFKVKN AYEESLDHLE 

      1510       1520       1530       1540       1550       1560 
TLKRENKNLQ QEISDLTEQI AEGGKHIHEL EKVKKQLDHE KSELQTSLEE AEASLEHEEG 

      1570       1580       1590       1600       1610       1620 
KILRIQLELN QVKSEIDRKI AEKDEELDQL KRNHLRVVES MQSTLDAEIR SRNDALRIKK 

      1630       1640       1650       1660       1670       1680 
KMEGDLNEME IQLNHANRQA AEALRNLRNT QGILKDTQLH LDDAIRGQDD LKEQLAMVER 

      1690       1700       1710       1720       1730       1740 
RANLMQAEVE ELRASLERTE RGRKMAEQEL LDASERVQLL HTQNTSLINT KKKLETDISQ 

      1750       1760       1770       1780       1790       1800 
IQGEMEDIVQ EARNAEEKAK KAITDAAMMA EELKKEQDTS AHLERMKKNM EQTVKDLQLR 

      1810       1820       1830       1840       1850       1860 
LDEAEQLALK GGKKQIQKLE ARVRELESEV ESEQKHNVEA VKGLRKHERR VKELTYQTEE 

      1870       1880       1890       1900       1910       1920 
DRKNILRLQD LVDKLQTKVK AYKRQAEEAE EQSNVNLAKF RKLQHELEEA KERADIAESQ 

      1930 
VNKLRVKSRE VHTKVISEE 

« Hide

References

« Hide 'large scale' references
[1]"Comparative sequence analysis of the complete human sarcomeric myosin heavy chain family: implications for functional diversity."
Weiss A., Schiaffino S., Leinwand L.A.
J. Mol. Biol. 290:61-75(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS MET-1106; LYS-1209; GLY-1802 AND GLU-1911.
Tissue: Skeletal muscle.
[2]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry."
Denis N.J., Vasilescu J., Lambert J.-P., Smith J.C., Figeys D.
Proteomics 7:868-874(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-1813.
Tissue: Mammary cancer.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF111783 mRNA. Translation: AAD29949.1.
AC005323 Genomic DNA. No translation available.
RefSeqNP_060003.2. NM_017533.2.
UniGeneHs.711141.

3D structure databases

ProteinModelPortalQ9Y623.
SMRQ9Y623. Positions 4-967.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110707. 4 interactions.
IntActQ9Y623. 3 interactions.
MINTMINT-5003666.
STRING9606.ENSP00000255381.

PTM databases

PhosphoSiteQ9Y623.

Polymorphism databases

DMDM224471840.

2D gel databases

UCD-2DPAGEQ9Y623.

Proteomic databases

PaxDbQ9Y623.
PRIDEQ9Y623.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000255381; ENSP00000255381; ENSG00000264424.
GeneID4622.
KEGGhsa:4622.
UCSCuc002gmn.3. human.

Organism-specific databases

CTD4622.
GeneCardsGC17M010346.
H-InvDBHIX0039061.
HGNCHGNC:7574. MYH4.
HPAHPA001239.
HPA001349.
MIM160742. gene.
neXtProtNX_Q9Y623.
PharmGKBPA31371.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5022.
HOGENOMHOG000173959.
HOVERGENHBG004704.
InParanoidQ9Y623.
KOK10352.
OMANTQGMLK.
OrthoDBEOG7RBZ7G.
PhylomeDBQ9Y623.
TreeFamTF314375.

Enzyme and pathway databases

ReactomeREACT_11123. Membrane Trafficking.

Gene expression databases

BgeeQ9Y623.
CleanExHS_MYH4.
GenevestigatorQ9Y623.

Family and domain databases

Gene3D4.10.270.10. 1 hit.
InterProIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR015650. Myosin_1/23/4/6/7/8/13/15.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR13140:SF22. PTHR13140:SF22. 1 hit.
PfamPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSPR00193. MYOSINHEAVY.
SMARTSM00015. IQ. 2 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS50096. IQ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiMYH4.
GenomeRNAi4622.
NextBio17794.
PROQ9Y623.
SOURCESearch...

Entry information

Entry nameMYH4_HUMAN
AccessionPrimary (citable) accession number: Q9Y623
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 3, 2009
Last modified: April 16, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM