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Protein

Beta-1,3-galactosyltransferase 1

Gene

B3GALT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Beta-1,3-galactosyltransferase that transfers galactose from UDP-alpha-D-galactose to substrates with a terminal beta-N-acetylglucosamine (beta-GlcNAc) residue. Involved in the biosynthesis of the carbohydrate moieties of glycolipids and glycoproteins. Inactive towards substrates with terminal alpha-N-acetylglucosamine (alpha-GlcNAc) or alpha-N-acetylgalactosamine (alpha-GalNAc) residues.1 Publication

Catalytic activityi

UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminyl-R = UDP + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R.1 Publication

Cofactori

Mn2+By similarity

Kineticsi

  1. KM=90 µM for UDP-alpha-D-galactose1 Publication

    Pathwayi: protein glycosylation

    This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
    View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

    GO - Molecular functioni

    GO - Biological processi

    • galactosylceramide biosynthetic process Source: BHF-UCL
    • lipid glycosylation Source: UniProtKB
    • oligosaccharide biosynthetic process Source: UniProtKB
    • protein glycosylation Source: UniProtKB-UniPathway

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase
    LigandManganese

    Enzyme and pathway databases

    BRENDAi2.4.1.134. 2681.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT31. Glycosyltransferase Family 31.

    Chemistry databases

    SwissLipidsiSLP:000000774.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,3-galactosyltransferase 1 (EC:2.4.1.861 Publication)
    Short name:
    Beta-1,3-GalTase 1
    Short name:
    Beta3Gal-T1
    Short name:
    Beta3GalT1
    Alternative name(s):
    UDP-galactose:beta-N-acetyl-glucosamine-beta-1,3-galactosyltransferase 1
    Gene namesi
    Name:B3GALT1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 2

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000172318.5.
    HGNCiHGNC:916. B3GALT1.

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 6CytoplasmicSequence analysis6
    Transmembranei7 – 26Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST20
    Topological domaini27 – 326LumenalSequence analysisAdd BLAST300

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi8708.
    OpenTargetsiENSG00000172318.
    PharmGKBiPA25209.

    Chemistry databases

    ChEMBLiCHEMBL2321634.

    Polymorphism and mutation databases

    BioMutaiB3GALT1.
    DMDMi61212254.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002191451 – 326Beta-1,3-galactosyltransferase 1Add BLAST326

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi47N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi151N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    EPDiQ9Y5Z6.
    PaxDbiQ9Y5Z6.
    PeptideAtlasiQ9Y5Z6.
    PRIDEiQ9Y5Z6.

    PTM databases

    iPTMnetiQ9Y5Z6.
    PhosphoSitePlusiQ9Y5Z6.

    Expressioni

    Tissue specificityi

    Detected in brain and colon mucosa and to a lesser extent in colon adenocarcinoma cells.2 Publications

    Gene expression databases

    BgeeiENSG00000172318.
    CleanExiHS_B3GALT1.
    GenevisibleiQ9Y5Z6. HS.

    Interactioni

    Protein-protein interaction databases

    BioGridi114251. 1 interactor.
    STRINGi9606.ENSP00000303740.

    Chemistry databases

    BindingDBiQ9Y5Z6.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Y5Z6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 31 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG2287. Eukaryota.
    ENOG410ZZ1B. LUCA.
    GeneTreeiENSGT00760000118879.
    HOGENOMiHOG000059604.
    HOVERGENiHBG101354.
    InParanoidiQ9Y5Z6.
    KOiK07819.
    OMAiHPYQNSG.
    OrthoDBiEOG091G0AXM.
    PhylomeDBiQ9Y5Z6.
    TreeFamiTF318639.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiView protein in InterPro
    IPR002659. Glyco_trans_31.
    IPR029044. Nucleotide-diphossugar_trans.
    PANTHERiPTHR11214. PTHR11214. 1 hit.
    PfamiView protein in Pfam
    PF01762. Galactosyl_T. 1 hit.
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9Y5Z6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MASKVSCLYV LTVVCWASAL WYLSITRPTS SYTGSKPFSH LTVARKNFTF
    60 70 80 90 100
    GNIRTRPINP HSFEFLINEP NKCEKNIPFL VILISTTHKE FDARQAIRET
    110 120 130 140 150
    WGDENNFKGI KIATLFLLGK NADPVLNQMV EQESQIFHDI IVEDFIDSYH
    160 170 180 190 200
    NLTLKTLMGM RWVATFCSKA KYVMKTDSDI FVNMDNLIYK LLKPSTKPRR
    210 220 230 240 250
    RYFTGYVING GPIRDVRSKW YMPRDLYPDS NYPPFCSGTG YIFSADVAEL
    260 270 280 290 300
    IYKTSLHTRL LHLEDVYVGL CLRKLGIHPF QNSGFNHWKM AYSLCRYRRV
    310 320
    ITVHQISPEE MHRIWNDMSS KKHLRC
    Length:326
    Mass (Da):37,993
    Last modified:November 1, 1999 - v1
    Checksum:i83271E99B2EE74F5
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF117222 mRNA. Translation: AAD23451.1.
    AB041407 Genomic DNA. Translation: BAA94492.1.
    AC016723 Genomic DNA. Translation: AAY15002.1.
    CH471058 Genomic DNA. Translation: EAX11307.1.
    BC101545 mRNA. Translation: AAI01546.1.
    BC104813 mRNA. Translation: AAI04814.1.
    CCDSiCCDS2227.1.
    RefSeqiNP_066191.1. NM_020981.3.
    XP_005246988.1. XM_005246931.3.
    XP_006712882.1. XM_006712819.3.
    XP_011510387.1. XM_011512085.2.
    UniGeneiHs.735833.

    Genome annotation databases

    EnsembliENST00000392690; ENSP00000376456; ENSG00000172318.
    GeneIDi8708.
    KEGGihsa:8708.
    UCSCiuc061pgb.1. human.

    Similar proteinsi

    Entry informationi

    Entry nameiB3GT1_HUMAN
    AccessioniPrimary (citable) accession number: Q9Y5Z6
    Secondary accession number(s): D3DPB8, Q53SS2
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: November 1, 1999
    Last modified: October 25, 2017
    This is version 126 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families