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Protein

Cytosolic Fe-S cluster assembly factor NUBP2

Gene

NUBP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Negatively regulates cilium formation and structure.UniRule annotationBy similarity

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi196Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1
Metal bindingi199Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 29ATPUniRule annotation8

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • nucleotide binding Source: ProtInc

GO - Biological processi

Keywordsi

Biological processCilium biogenesis/degradation
Ligand4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-2564830 Cytosolic iron-sulfur cluster assembly

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor NUBP2UniRule annotation
Alternative name(s):
Nucleotide-binding protein 2UniRule annotation
Short name:
NBP 2UniRule annotation
Gene namesi
Name:NUBP2UniRule annotation
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000095906.16
HGNCiHGNC:8042 NUBP2
MIMi610779 gene
neXtProtiNX_Q9Y5Y2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10101
OpenTargetsiENSG00000095906
PharmGKBiPA31824

Polymorphism and mutation databases

DMDMi13632176

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001849451 – 271Cytosolic Fe-S cluster assembly factor NUBP2Add BLAST271

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineUniRule annotationCombined sources1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9Y5Y2
MaxQBiQ9Y5Y2
PaxDbiQ9Y5Y2
PeptideAtlasiQ9Y5Y2
PRIDEiQ9Y5Y2
ProteomicsDBi86538

PTM databases

iPTMnetiQ9Y5Y2
PhosphoSitePlusiQ9Y5Y2

Expressioni

Tissue specificityi

Widely expressed with highest expression in skeletal muscle.

Developmental stagei

Expressed in fetal brain, lung, liver and kidney.

Gene expression databases

BgeeiENSG00000095906
CleanExiHS_NUBP2
ExpressionAtlasiQ9Y5Y2 baseline and differential

Organism-specific databases

HPAiHPA041704

Interactioni

Subunit structurei

Heterotetramer of 2 NUBP1 and 2 NUBP2 chains (By similarity). Interacts with KIFC1 (By similarity). Interacts with NUBP1 (PubMed:18573874).UniRule annotationBy similarity1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi115408, 65 interactors
IntActiQ9Y5Y2, 11 interactors
STRINGi9606.ENSP00000262302

Structurei

3D structure databases

ProteinModelPortaliQ9Y5Y2
SMRiQ9Y5Y2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP2/CFD1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG410KCSJ Eukaryota
COG0489 LUCA
GeneTreeiENSGT00550000075057
HOGENOMiHOG000079916
HOVERGENiHBG051027
InParanoidiQ9Y5Y2
OMAiRELTFCK
PhylomeDBiQ9Y5Y2
TreeFamiTF354321

Family and domain databases

CDDicd02037 MRP-like, 1 hit
HAMAPiMF_02040 Mrp_NBP35, 1 hit
MF_03039 NUBP2, 1 hit
InterProiView protein in InterPro
IPR019591 Mrp/NBP35_ATP-bd
IPR000808 Mrp_CS
IPR028600 NUBP2/Cfd1_eukaryotes
IPR027417 P-loop_NTPase
IPR033756 YlxH/NBP35
PANTHERiPTHR23264 PTHR23264, 1 hit
PfamiView protein in Pfam
PF10609 ParA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS01215 MRP, 1 hit

Sequencei

Sequence statusi: Complete.

Q9Y5Y2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAAAEPGNL AGVRHIILVL SGKGGVGKST ISTELALALR HAGKKVGILD
60 70 80 90 100
VDLCGPSIPR MLGAQGRAVH QCDRGWAPVF LDREQSISLM SVGFLLEKPD
110 120 130 140 150
EAVVWRGPKK NALIKQFVSD VAWGELDYLV VDTPPGTSDE HMATIEALRP
160 170 180 190 200
YQPLGALVVT TPQAVSVGDV RRELTFCRKT GLRVMGIVEN MSGFTCPHCT
210 220 230 240 250
ECTSVFSRGG GEELAQLAGV PFLGSVPLDP ALMRTLEEGH DFIQEFPGSP
260 270
AFAALTSIAQ KILDATPACL P
Length:271
Mass (Da):28,825
Last modified:November 1, 1999 - v1
Checksum:i3B1AB82C4FE9C8EE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti242F → S in BAA91471 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_061353200T → A. Corresponds to variant dbSNP:rs57822546Ensembl.1
Natural variantiVAR_050099250P → S. Corresponds to variant dbSNP:rs35030308Ensembl.1
Natural variantiVAR_050100266T → M. Corresponds to variant dbSNP:rs34028164Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF118394 mRNA Translation: AAD45242.1
AK001023 mRNA Translation: BAA91471.1
CH471112 Genomic DNA Translation: EAW85613.1
CH471112 Genomic DNA Translation: EAW85617.1
BC002768 mRNA Translation: AAH02768.1
BC008005 mRNA Translation: AAH08005.1
CCDSiCCDS10445.1
RefSeqiNP_001271430.1, NM_001284501.1
NP_036357.1, NM_012225.3
UniGeneiHs.256549

Genome annotation databases

EnsembliENST00000262302; ENSP00000262302; ENSG00000095906
GeneIDi10101
KEGGihsa:10101
UCSCiuc002cmw.6 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiNUBP2_HUMAN
AccessioniPrimary (citable) accession number: Q9Y5Y2
Secondary accession number(s): D3DU80, Q9NWB2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1999
Last modified: June 20, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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