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Q9Y5W5 (WIF1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Wnt inhibitory factor 1

Short name=WIF-1
Gene names
Name:WIF1
ORF Names:UNQ191/PRO217
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length379 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.

Subunit structure

Interacts with MYOC. Ref.4

Subcellular location

Secreted.

Sequence similarities

Contains 5 EGF-like domains.

Contains 1 WIF domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Wnt3aP274677EBI-3922719,EBI-2899665From a different organism.
Wnt5aP227257EBI-3922719,EBI-1570983From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 379351Wnt inhibitory factor 1
PRO_0000007775

Regions

Domain38 – 177140WIF
Domain178 – 21033EGF-like 1
Domain211 – 24232EGF-like 2
Domain243 – 27129EGF-like 3
Domain274 – 30633EGF-like 4
Domain307 – 33832EGF-like 5

Amino acid modifications

Glycosylation881N-linked (GlcNAc...) Potential
Glycosylation2451N-linked (GlcNAc...) Potential
Disulfide bond140 ↔ 177 Ref.5
Disulfide bond182 ↔ 192 By similarity
Disulfide bond186 ↔ 198 By similarity
Disulfide bond200 ↔ 209 By similarity
Disulfide bond214 ↔ 224 By similarity
Disulfide bond218 ↔ 230 By similarity
Disulfide bond232 ↔ 241 By similarity
Disulfide bond246 ↔ 256 By similarity
Disulfide bond250 ↔ 262 By similarity
Disulfide bond278 ↔ 288 By similarity
Disulfide bond282 ↔ 294 By similarity
Disulfide bond296 ↔ 305 By similarity
Disulfide bond310 ↔ 320 By similarity
Disulfide bond314 ↔ 326 By similarity
Disulfide bond328 ↔ 337 By similarity

Experimental info

Sequence conflict1661Q → K in AAH18037. Ref.3
Sequence conflict1781Q → L in AAD25402. Ref.1

Secondary structure

................................... 379
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9Y5W5 [UniParc].

Last modified March 15, 2005. Version 3.
Checksum: 32ADFA6644833E9D

FASTA37941,528
        10         20         30         40         50         60 
MARRSAFPAA ALWLWSILLC LLALRAEAGP PQEESLYLWI DAHQARVLIG FEEDILIVSE 

        70         80         90        100        110        120 
GKMAPFTHDF RKAQQRMPAI PVNIHSMNFT WQAAGQAEYF YEFLSLRSLD KGIMADPTVN 

       130        140        150        160        170        180 
VPLLGTVPHK ASVVQVGFPC LGKQDGVAAF EVDVIVMNSE GNTILQTPQN AIFFKTCQQA 

       190        200        210        220        230        240 
ECPGGCRNGG FCNERRICEC PDGFHGPHCE KALCTPRCMN GGLCVTPGFC ICPPGFYGVN 

       250        260        270        280        290        300 
CDKANCSTTC FNGGTCFYPG KCICPPGLEG EQCEISKCPQ PCRNGGKCIG KSKCKCSKGY 

       310        320        330        340        350        360 
QGDLCSKPVC EPGCGAHGTC HEPNKCQCQE GWHGRHCNKR YEASLIHALR PAGAQLRQHT 

       370 
PSLKKAEERR DPPESNYIW 

« Hide

References

« Hide 'large scale' references
[1]"A new secreted protein that binds to Wnt proteins and inhibits their activities."
Hsieh J.-C., Kodjabachian L., Rebbert M.L., Rattner A., Smallwood P.M., Samos C.H., Nusse R., Dawid I.B., Nathans J.
Nature 398:431-436(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"Myocilin is a modulator of Wnt signaling."
Kwon H.S., Lee H.S., Ji Y., Rubin J.S., Tomarev S.I.
Mol. Cell. Biol. 29:2139-2154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MYOC.
[5]"NMR structure of the WIF domain of the human Wnt-inhibitory factor-1."
Liepinsh E., Banyai L., Patthy L., Otting G.
J. Mol. Biol. 357:942-950(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 32-181, DISULFIDE BOND.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF122922 mRNA. Translation: AAD25402.1.
AY358344 mRNA. Translation: AAQ88710.1.
BC018037 mRNA. Translation: AAH18037.1.
CCDSCCDS8971.1.
PIRA59180.
RefSeqNP_009122.2. NM_007191.4.
UniGeneHs.284122.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2D3JNMR-A32-181[»]
2YGNX-ray1.85A35-178[»]
2YGOX-ray1.85A35-210[»]
2YGPX-ray2.22A35-210[»]
2YGQX-ray3.95A35-346[»]
ProteinModelPortalQ9Y5W5.
SMRQ9Y5W5. Positions 35-339.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116366. 3 interactions.
DIPDIP-59097N.
IntActQ9Y5W5. 8 interactions.
MINTMINT-8389706.
STRING9606.ENSP00000286574.

Polymorphism databases

DMDM61252765.

Proteomic databases

PaxDbQ9Y5W5.
PRIDEQ9Y5W5.

Protocols and materials databases

DNASU11197.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000286574; ENSP00000286574; ENSG00000156076.
GeneID11197.
KEGGhsa:11197.
UCSCuc001ssk.3. human.

Organism-specific databases

CTD11197.
GeneCardsGC12M065444.
HGNCHGNC:18081. WIF1.
HPAHPA039104.
MIM605186. gene.
neXtProtNX_Q9Y5W5.
PharmGKBPA38291.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG328460.
HOVERGENHBG017335.
InParanoidQ9Y5W5.
KOK01691.
OMADKGIMAD.
OrthoDBEOG7VQJD2.
PhylomeDBQ9Y5W5.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
SignaLinkQ9Y5W5.

Gene expression databases

ArrayExpressQ9Y5W5.
BgeeQ9Y5W5.
CleanExHS_WIF1.
GenevestigatorQ9Y5W5.

Family and domain databases

InterProIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR003306. WIF.
IPR013309. Wnt-inh.
[Graphical view]
PfamPF12661. hEGF. 4 hits.
PF02019. WIF. 1 hit.
[Graphical view]
PRINTSPR01901. WIFPROTEIN.
ProDomPD013948. WIF. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00181. EGF. 5 hits.
SM00469. WIF. 1 hit.
[Graphical view]
PROSITEPS00022. EGF_1. 5 hits.
PS01186. EGF_2. 4 hits.
PS50026. EGF_3. 5 hits.
PS50814. WIF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9Y5W5.
GeneWikiWIF1.
GenomeRNAi11197.
NextBio42617.
PROQ9Y5W5.
SOURCESearch...

Entry information

Entry nameWIF1_HUMAN
AccessionPrimary (citable) accession number: Q9Y5W5
Secondary accession number(s): Q6UXI1, Q8WVG4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: March 15, 2005
Last modified: July 9, 2014
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM