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Q9Y5W5

- WIF1_HUMAN

UniProt

Q9Y5W5 - WIF1_HUMAN

Protein

Wnt inhibitory factor 1

Gene

WIF1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 3 (15 Mar 2005)
      Previous versions | rss
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    Functioni

    Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. multicellular organismal development Source: UniProtKB-KW
    2. positive regulation of fat cell differentiation Source: Ensembl
    3. signal transduction Source: ProtInc
    4. Wnt signaling pathway Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Wnt signaling pathway

    Enzyme and pathway databases

    ReactomeiREACT_200643. negative regulation of TCF-dependent signaling by WNT ligand antagonists.
    SignaLinkiQ9Y5W5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Wnt inhibitory factor 1
    Short name:
    WIF-1
    Gene namesi
    Name:WIF1
    ORF Names:UNQ191/PRO217
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:18081. WIF1.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA38291.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Chaini29 – 379351Wnt inhibitory factor 1PRO_0000007775Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi88 – 881N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi140 ↔ 1771 Publication
    Disulfide bondi182 ↔ 192By similarity
    Disulfide bondi186 ↔ 198By similarity
    Disulfide bondi200 ↔ 209By similarity
    Disulfide bondi214 ↔ 224By similarity
    Disulfide bondi218 ↔ 230By similarity
    Disulfide bondi232 ↔ 241By similarity
    Glycosylationi245 – 2451N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi246 ↔ 256By similarity
    Disulfide bondi250 ↔ 262By similarity
    Disulfide bondi278 ↔ 288By similarity
    Disulfide bondi282 ↔ 294By similarity
    Disulfide bondi296 ↔ 305By similarity
    Disulfide bondi310 ↔ 320By similarity
    Disulfide bondi314 ↔ 326By similarity
    Disulfide bondi328 ↔ 337By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ9Y5W5.
    PRIDEiQ9Y5W5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9Y5W5.
    BgeeiQ9Y5W5.
    CleanExiHS_WIF1.
    GenevestigatoriQ9Y5W5.

    Organism-specific databases

    HPAiHPA039104.

    Interactioni

    Subunit structurei

    Interacts with MYOC.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Wnt3aP274677EBI-3922719,EBI-2899665From a different organism.
    Wnt5aP227257EBI-3922719,EBI-1570983From a different organism.

    Protein-protein interaction databases

    BioGridi116366. 3 interactions.
    DIPiDIP-59097N.
    IntActiQ9Y5W5. 8 interactions.
    MINTiMINT-8389706.
    STRINGi9606.ENSP00000286574.

    Structurei

    Secondary structure

    1
    379
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi36 – 405
    Helixi42 – 498
    Beta strandi55 – 595
    Helixi65 – 673
    Helixi70 – 756
    Beta strandi85 – 939
    Beta strandi95 – 973
    Beta strandi99 – 10911
    Turni111 – 1133
    Beta strandi118 – 1214
    Beta strandi123 – 1264
    Beta strandi132 – 1376
    Beta strandi142 – 1443
    Beta strandi146 – 15813
    Beta strandi163 – 1664
    Beta strandi173 – 1775
    Turni206 – 2094

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2D3JNMR-A32-181[»]
    2YGNX-ray1.85A35-178[»]
    2YGOX-ray1.85A35-210[»]
    2YGPX-ray2.22A35-210[»]
    2YGQX-ray3.95A35-346[»]
    ProteinModelPortaliQ9Y5W5.
    SMRiQ9Y5W5. Positions 35-339.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9Y5W5.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini38 – 177140WIFPROSITE-ProRule annotationAdd
    BLAST
    Domaini178 – 21033EGF-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini211 – 24232EGF-like 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini243 – 27129EGF-like 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini274 – 30633EGF-like 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini307 – 33832EGF-like 5PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 5 EGF-like domains.PROSITE-ProRule annotation
    Contains 1 WIF domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG328460.
    HOVERGENiHBG017335.
    InParanoidiQ9Y5W5.
    KOiK01691.
    OMAiDKGIMAD.
    OrthoDBiEOG7VQJD2.
    PhylomeDBiQ9Y5W5.

    Family and domain databases

    InterProiIPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR003306. WIF.
    IPR013309. Wnt-inh.
    [Graphical view]
    PfamiPF12661. hEGF. 4 hits.
    PF02019. WIF. 1 hit.
    [Graphical view]
    PRINTSiPR01901. WIFPROTEIN.
    ProDomiPD013948. WIF. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00181. EGF. 5 hits.
    SM00469. WIF. 1 hit.
    [Graphical view]
    PROSITEiPS00022. EGF_1. 5 hits.
    PS01186. EGF_2. 4 hits.
    PS50026. EGF_3. 5 hits.
    PS50814. WIF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9Y5W5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARRSAFPAA ALWLWSILLC LLALRAEAGP PQEESLYLWI DAHQARVLIG    50
    FEEDILIVSE GKMAPFTHDF RKAQQRMPAI PVNIHSMNFT WQAAGQAEYF 100
    YEFLSLRSLD KGIMADPTVN VPLLGTVPHK ASVVQVGFPC LGKQDGVAAF 150
    EVDVIVMNSE GNTILQTPQN AIFFKTCQQA ECPGGCRNGG FCNERRICEC 200
    PDGFHGPHCE KALCTPRCMN GGLCVTPGFC ICPPGFYGVN CDKANCSTTC 250
    FNGGTCFYPG KCICPPGLEG EQCEISKCPQ PCRNGGKCIG KSKCKCSKGY 300
    QGDLCSKPVC EPGCGAHGTC HEPNKCQCQE GWHGRHCNKR YEASLIHALR 350
    PAGAQLRQHT PSLKKAEERR DPPESNYIW 379
    Length:379
    Mass (Da):41,528
    Last modified:March 15, 2005 - v3
    Checksum:i32ADFA6644833E9D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti166 – 1661Q → K in AAH18037. (PubMed:15489334)Curated
    Sequence conflicti178 – 1781Q → L in AAD25402. (PubMed:10201374)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF122922 mRNA. Translation: AAD25402.1.
    AY358344 mRNA. Translation: AAQ88710.1.
    BC018037 mRNA. Translation: AAH18037.1.
    CCDSiCCDS8971.1.
    PIRiA59180.
    RefSeqiNP_009122.2. NM_007191.4.
    UniGeneiHs.284122.

    Genome annotation databases

    EnsembliENST00000286574; ENSP00000286574; ENSG00000156076.
    GeneIDi11197.
    KEGGihsa:11197.
    UCSCiuc001ssk.3. human.

    Polymorphism databases

    DMDMi61252765.

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF122922 mRNA. Translation: AAD25402.1 .
    AY358344 mRNA. Translation: AAQ88710.1 .
    BC018037 mRNA. Translation: AAH18037.1 .
    CCDSi CCDS8971.1.
    PIRi A59180.
    RefSeqi NP_009122.2. NM_007191.4.
    UniGenei Hs.284122.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2D3J NMR - A 32-181 [» ]
    2YGN X-ray 1.85 A 35-178 [» ]
    2YGO X-ray 1.85 A 35-210 [» ]
    2YGP X-ray 2.22 A 35-210 [» ]
    2YGQ X-ray 3.95 A 35-346 [» ]
    ProteinModelPortali Q9Y5W5.
    SMRi Q9Y5W5. Positions 35-339.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 116366. 3 interactions.
    DIPi DIP-59097N.
    IntActi Q9Y5W5. 8 interactions.
    MINTi MINT-8389706.
    STRINGi 9606.ENSP00000286574.

    Polymorphism databases

    DMDMi 61252765.

    Proteomic databases

    PaxDbi Q9Y5W5.
    PRIDEi Q9Y5W5.

    Protocols and materials databases

    DNASUi 11197.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000286574 ; ENSP00000286574 ; ENSG00000156076 .
    GeneIDi 11197.
    KEGGi hsa:11197.
    UCSCi uc001ssk.3. human.

    Organism-specific databases

    CTDi 11197.
    GeneCardsi GC12M065444.
    HGNCi HGNC:18081. WIF1.
    HPAi HPA039104.
    MIMi 605186. gene.
    neXtProti NX_Q9Y5W5.
    PharmGKBi PA38291.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG328460.
    HOVERGENi HBG017335.
    InParanoidi Q9Y5W5.
    KOi K01691.
    OMAi DKGIMAD.
    OrthoDBi EOG7VQJD2.
    PhylomeDBi Q9Y5W5.

    Enzyme and pathway databases

    Reactomei REACT_200643. negative regulation of TCF-dependent signaling by WNT ligand antagonists.
    SignaLinki Q9Y5W5.

    Miscellaneous databases

    EvolutionaryTracei Q9Y5W5.
    GeneWikii WIF1.
    GenomeRNAii 11197.
    NextBioi 42617.
    PROi Q9Y5W5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Y5W5.
    Bgeei Q9Y5W5.
    CleanExi HS_WIF1.
    Genevestigatori Q9Y5W5.

    Family and domain databases

    InterProi IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR003306. WIF.
    IPR013309. Wnt-inh.
    [Graphical view ]
    Pfami PF12661. hEGF. 4 hits.
    PF02019. WIF. 1 hit.
    [Graphical view ]
    PRINTSi PR01901. WIFPROTEIN.
    ProDomi PD013948. WIF. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00181. EGF. 5 hits.
    SM00469. WIF. 1 hit.
    [Graphical view ]
    PROSITEi PS00022. EGF_1. 5 hits.
    PS01186. EGF_2. 4 hits.
    PS50026. EGF_3. 5 hits.
    PS50814. WIF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A new secreted protein that binds to Wnt proteins and inhibits their activities."
      Hsieh J.-C., Kodjabachian L., Rebbert M.L., Rattner A., Smallwood P.M., Samos C.H., Nusse R., Dawid I.B., Nathans J.
      Nature 398:431-436(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    4. Cited for: INTERACTION WITH MYOC.
    5. "NMR structure of the WIF domain of the human Wnt-inhibitory factor-1."
      Liepinsh E., Banyai L., Patthy L., Otting G.
      J. Mol. Biol. 357:942-950(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 32-181, DISULFIDE BOND.

    Entry informationi

    Entry nameiWIF1_HUMAN
    AccessioniPrimary (citable) accession number: Q9Y5W5
    Secondary accession number(s): Q6UXI1, Q8WVG4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: March 15, 2005
    Last modified: October 1, 2014
    This is version 131 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3