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Protein

Krueppel-like factor 2

Gene

KLF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the CACCC box in the beta-globin gene promoter and activates transcription.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri272 – 29625C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri302 – 32625C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri332 – 35423C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. sequence-specific DNA binding Source: Ensembl
  3. sequence-specific DNA binding transcription factor activity Source: Ensembl

GO - Biological processi

  1. cell morphogenesis Source: Ensembl
  2. cellular response to cycloheximide Source: Ensembl
  3. cellular response to hydrogen peroxide Source: Ensembl
  4. cellular response to interleukin-1 Source: Ensembl
  5. cellular response to laminar fluid shear stress Source: BHF-UCL
  6. cellular response to peptide Source: Ensembl
  7. cellular response to tumor necrosis factor Source: Ensembl
  8. erythrocyte maturation Source: Ensembl
  9. in utero embryonic development Source: Ensembl
  10. multicellular organism growth Source: Ensembl
  11. negative regulation of interleukin-6 production Source: Ensembl
  12. positive regulation of protein metabolic process Source: BHF-UCL
  13. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  14. positive regulation of transcription from RNA polymerase II promoter in response to stress Source: BHF-UCL
  15. regulation of gene expression, epigenetic Source: Ensembl
  16. transcription, DNA-templated Source: UniProtKB-KW
  17. Type I pneumocyte differentiation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Krueppel-like factor 2
Alternative name(s):
Lung krueppel-like factor
Gene namesi
Name:KLF2
Synonyms:LKLF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:6347. KLF2.

Subcellular locationi

  1. Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30136.

Polymorphism and mutation databases

BioMutaiKLF2.
DMDMi20141620.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 355355Krueppel-like factor 2PRO_0000047162Add
BLAST

Post-translational modificationi

Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein (By similarity).By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ9Y5W3.
PRIDEiQ9Y5W3.

PTM databases

PhosphoSiteiQ9Y5W3.

Expressioni

Gene expression databases

BgeeiQ9Y5W3.
CleanExiHS_KLF2.
ExpressionAtlasiQ9Y5W3. baseline and differential.
GenevestigatoriQ9Y5W3.

Organism-specific databases

HPAiHPA055964.

Interactioni

Subunit structurei

Interacts with WWP1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SMURF1Q9HCE72EBI-9846663,EBI-976466
SMURF1Q9HCE7-24EBI-9846663,EBI-9845742

Protein-protein interaction databases

BioGridi115645. 11 interactions.
DIPiDIP-41973N.
IntActiQ9Y5W3. 1 interaction.
MINTiMINT-199597.
STRINGi9606.ENSP00000248071.

Structurei

3D structure databases

ProteinModelPortaliQ9Y5W3.
SMRiQ9Y5W3. Positions 269-354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni111 – 268158Interaction with WWP1By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi62 – 7110Poly-Pro
Compositional biasi130 – 1356Poly-Gly
Compositional biasi167 – 1715Poly-Pro
Compositional biasi225 – 2317Poly-Ala

Sequence similaritiesi

Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri272 – 29625C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri302 – 32625C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri332 – 35423C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000118998.
HOGENOMiHOG000060173.
HOVERGENiHBG006220.
InParanoidiQ9Y5W3.
KOiK17845.
OMAiFYYPEPG.
PhylomeDBiQ9Y5W3.
TreeFamiTF350556.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Y5W3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSEPILPS FSTFASPCRE RGLQERWPRA EPESGGTDDD LNSVLDFILS
60 70 80 90 100
MGLDGLGAEA APEPPPPPPP PAFYYPEPGA PPPYSAPAGG LVSELLRPEL
110 120 130 140 150
DAPLGPALHG RFLLAPPGRL VKAEPPEADG GGGYGCAPGL TRGPRGLKRE
160 170 180 190 200
GAPGPAASCM RGPGGRPPPP PDTPPLSPDG PARLPAPGPR ASFPPPFGGP
210 220 230 240 250
GFGAPGPGLH YAPPAPPAFG LFDDAAAAAA ALGLAPPAAR GLLTPPASPL
260 270 280 290 300
ELLEAKPKRG RRSWPRKRTA THTCSYAGCG KTYTKSSHLK AHLRTHTGEK
310 320 330 340 350
PYHCNWDGCG WKFARSDELT RHYRKHTGHR PFQCHLCDRA FSRSDHLALH

MKRHM
Length:355
Mass (Da):37,420
Last modified:January 23, 2002 - v2
Checksum:iD5849C831D676AE1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti43 – 431S → N in AAD55891 (PubMed:10458913).Curated
Sequence conflicti175 – 1751P → S in AAD55891 (PubMed:10458913).Curated
Sequence conflicti184 – 1841L → M in AAD55891 (PubMed:10458913).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti104 – 1041L → P.3 Publications
Corresponds to variant rs3745318 [ dbSNP | Ensembl ].
VAR_038830
Natural varianti145 – 1451R → P.1 Publication
Corresponds to variant rs45586032 [ dbSNP | Ensembl ].
VAR_038831

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF123344 Genomic DNA. Translation: AAD25076.1.
AF134053 mRNA. Translation: AAD55891.1.
AF205849 mRNA. Translation: AAF13295.1.
EF078888 Genomic DNA. Translation: ABK41959.1.
BC071983 mRNA. Translation: AAH71983.1.
CCDSiCCDS12343.1.
RefSeqiNP_057354.1. NM_016270.2.
UniGeneiHs.744182.

Genome annotation databases

EnsembliENST00000248071; ENSP00000248071; ENSG00000127528.
GeneIDi10365.
KEGGihsa:10365.
UCSCiuc002ndw.3. human.

Polymorphism and mutation databases

BioMutaiKLF2.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

SeattleSNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF123344 Genomic DNA. Translation: AAD25076.1.
AF134053 mRNA. Translation: AAD55891.1.
AF205849 mRNA. Translation: AAF13295.1.
EF078888 Genomic DNA. Translation: ABK41959.1.
BC071983 mRNA. Translation: AAH71983.1.
CCDSiCCDS12343.1.
RefSeqiNP_057354.1. NM_016270.2.
UniGeneiHs.744182.

3D structure databases

ProteinModelPortaliQ9Y5W3.
SMRiQ9Y5W3. Positions 269-354.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115645. 11 interactions.
DIPiDIP-41973N.
IntActiQ9Y5W3. 1 interaction.
MINTiMINT-199597.
STRINGi9606.ENSP00000248071.

PTM databases

PhosphoSiteiQ9Y5W3.

Polymorphism and mutation databases

BioMutaiKLF2.
DMDMi20141620.

Proteomic databases

PaxDbiQ9Y5W3.
PRIDEiQ9Y5W3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000248071; ENSP00000248071; ENSG00000127528.
GeneIDi10365.
KEGGihsa:10365.
UCSCiuc002ndw.3. human.

Organism-specific databases

CTDi10365.
GeneCardsiGC19P016435.
HGNCiHGNC:6347. KLF2.
HPAiHPA055964.
MIMi602016. gene.
neXtProtiNX_Q9Y5W3.
PharmGKBiPA30136.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000118998.
HOGENOMiHOG000060173.
HOVERGENiHBG006220.
InParanoidiQ9Y5W3.
KOiK17845.
OMAiFYYPEPG.
PhylomeDBiQ9Y5W3.
TreeFamiTF350556.

Miscellaneous databases

ChiTaRSiKLF2. human.
GeneWikiiKLF2.
GenomeRNAii10365.
NextBioi39291.
PROiQ9Y5W3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Y5W3.
CleanExiHS_KLF2.
ExpressionAtlasiQ9Y5W3. baseline and differential.
GenevestigatoriQ9Y5W3.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of the human CpG-island-containing lung Kruppel-like factor (LKLF) gene and its location in chromosome 19p13.11-13 locus."
    Kozyrev S.V., Hansen L.L., Poltaraus A.B., Domninsky D.A., Kisselev L.L.
    FEBS Lett. 448:149-152(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT PRO-104.
    Tissue: Lung.
  2. "cDNA isolation, genomic structure, regulation, and chromosomal localization of human lung kruppel-like factor."
    Wani M.A., Conkright M.D., Jeffries S., Hughes M.J., Lingrel J.B.
    Genomics 60:78-86(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lung.
  3. "A catalogue of genes in the human dermal papilla cells as identified by expressed sequence tags."
    Lee H.J., Kim M.K., Kim Y.H., Seo J.M., Lee H.M., Chung H.J., Sohn M.Y., Hwang S.Y., Im S.U., Jung E.J., Kim J.C.
    Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Hair follicle dermal papilla.
  4. SeattleSNPs variation discovery resource
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS PRO-104 AND PRO-145.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-104.
    Tissue: Spleen.
  6. "Lung Krueppel-like factor contains an autoinhibitory domain that regulates its transcriptional activation by binding WWP1, an E3 ubiquitin ligase."
    Conkright M.D., Wani M.A., Lingrel J.B.
    J. Biol. Chem. 276:29299-29306(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH WWP1.

Entry informationi

Entry nameiKLF2_HUMAN
AccessioniPrimary (citable) accession number: Q9Y5W3
Secondary accession number(s): Q6IPC4, Q9UJS5, Q9UKR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2002
Last modified: April 29, 2015
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.