##gff-version 3
Q9Y5U4	UniProtKB	Chain	1	225	.	.	.	ID=PRO_0000286797;Note=Insulin-induced gene 2 protein	
Q9Y5U4	UniProtKB	Topological domain	1	28	.	.	.	Note=Cytoplasmic;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q9Y5U4	UniProtKB	Transmembrane	29	51	.	.	.	Note=Helical%3B Name%3D1;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:A1T557	
Q9Y5U4	UniProtKB	Topological domain	52	70	.	.	.	Note=Lumenal;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q9Y5U4	UniProtKB	Transmembrane	71	88	.	.	.	Note=Helical%3B Name%3D2;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:A1T557	
Q9Y5U4	UniProtKB	Topological domain	89	103	.	.	.	Note=Cytoplasmic;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q9Y5U4	UniProtKB	Transmembrane	104	126	.	.	.	Note=Helical%3B Name%3D3;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:A1T557	
Q9Y5U4	UniProtKB	Topological domain	127	129	.	.	.	Note=Lumenal;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q9Y5U4	UniProtKB	Transmembrane	130	148	.	.	.	Note=Helical%3B Name%3D4;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:A1T557	
Q9Y5U4	UniProtKB	Topological domain	149	153	.	.	.	Note=Cytoplasmic;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q9Y5U4	UniProtKB	Transmembrane	154	175	.	.	.	Note=Helical%3B Name%3D5;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:A1T557	
Q9Y5U4	UniProtKB	Topological domain	176	189	.	.	.	Note=Lumenal;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q9Y5U4	UniProtKB	Transmembrane	190	207	.	.	.	Note=Helical%3B Name%3D6;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:A1T557	
Q9Y5U4	UniProtKB	Topological domain	208	225	.	.	.	Note=Cytoplasmic;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q9Y5U4	UniProtKB	Motif	219	225	.	.	.	Note=KxHxx;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:23481256;Dbxref=PMID:23481256	
Q9Y5U4	UniProtKB	Site	115	115	.	.	.	Note=Required for the recognition of 25-hydroxycholesterol;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17428920;Dbxref=PMID:17428920	
Q9Y5U4	UniProtKB	Modified residue	151	151	.	.	.	Note=Phosphoserine%3B by PCK1;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32322062;Dbxref=PMID:32322062	
Q9Y5U4	UniProtKB	Modified residue	215	215	.	.	.	Note=Cysteine sulfenic acid (-SOH)%3B alternate;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:31953408;Dbxref=PMID:31953408	
Q9Y5U4	UniProtKB	Cross-link	215	215	.	.	.	Note=Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in ubiquitin)%3B alternate;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:31953408;Dbxref=PMID:31953408	
Q9Y5U4	UniProtKB	Mutagenesis	39	39	.	.	.	Note=Decreased binding to 25-hydroxycholesterol%2C leading to decreased interaction with SCAP. G->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26160948;Dbxref=PMID:26160948	
Q9Y5U4	UniProtKB	Mutagenesis	77	77	.	.	.	Note=Decreased binding to 25-hydroxycholesterol%2C leading to decreased interaction with SCAP. C->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26160948;Dbxref=PMID:26160948	
Q9Y5U4	UniProtKB	Mutagenesis	100	102	.	.	.	Note=Does not affect degradation of the protein. KFK->RFR;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:31953408;Dbxref=PMID:31953408	
Q9Y5U4	UniProtKB	Mutagenesis	113	113	.	.	.	Note=Abolished interaction with SCAP even in the presence of 25-hydroxycholesterol. A->W;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26160948;Dbxref=PMID:26160948	
Q9Y5U4	UniProtKB	Mutagenesis	114	114	.	.	.	Note=Does not affect interaction with SCAP. V->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26160948;Dbxref=PMID:26160948	
Q9Y5U4	UniProtKB	Mutagenesis	115	115	.	.	.	Note=Decreased binding to 25-hydroxycholesterol and subsequent interaction with SCAP. F->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:17428920,ECO:0000269|PubMed:26160948;Dbxref=PMID:17428920,PMID:26160948	
Q9Y5U4	UniProtKB	Mutagenesis	116	116	.	.	.	Note=Does not affect interaction with SCAP. V->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26160948;Dbxref=PMID:26160948	
Q9Y5U4	UniProtKB	Mutagenesis	117	117	.	.	.	Note=Abolished interaction with SCAP even in the presence of 25-hydroxycholesterol. G->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26160948;Dbxref=PMID:26160948	
Q9Y5U4	UniProtKB	Mutagenesis	120	120	.	.	.	Note=Abolished interaction with SCAP even in the presence of 25-hydroxycholesterol. H->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26160948;Dbxref=PMID:26160948	
Q9Y5U4	UniProtKB	Mutagenesis	132	132	.	.	.	Note=Abolished interaction with SCAP without affecting binding to 25-hydroxycholesterol. Q->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:17428920,ECO:0000269|PubMed:26160948;Dbxref=PMID:17428920,PMID:26160948	
Q9Y5U4	UniProtKB	Mutagenesis	136	136	.	.	.	Note=Decreased binding to 25-hydroxycholesterol and subsequent interaction with SCAP. T->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17428920;Dbxref=PMID:17428920	
Q9Y5U4	UniProtKB	Mutagenesis	145	145	.	.	.	Note=Abolished interaction with SCAP without affecting binding to 25-hydroxycholesterol. W->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:17428920,ECO:0000269|PubMed:26160948;Dbxref=PMID:17428920,PMID:26160948	
Q9Y5U4	UniProtKB	Mutagenesis	149	149	.	.	.	Note=Loss of ability to suppress the cleavage of SREBP2 and to accelerate the degradation of HMGCR. Abolished interaction with SCAP without affecting binding to 25-hydroxycholesterol. D->A;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:16606821,ECO:0000269|PubMed:17428920,ECO:0000269|PubMed:26160948;Dbxref=PMID:16606821,PMID:17428920,PMID:26160948	
Q9Y5U4	UniProtKB	Mutagenesis	151	151	.	.	.	Note=Abolished phosphorylation by PCK1%2C does not affect oxysterol-binding%2C does not affect the interaction with SCAP. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32322062;Dbxref=PMID:32322062	
Q9Y5U4	UniProtKB	Mutagenesis	151	151	.	.	.	Note=Phosphomimetic mutant%2C reduced binding to oxysterol. S->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32322062;Dbxref=PMID:32322062	
Q9Y5U4	UniProtKB	Mutagenesis	200	200	.	.	.	Note=Decreased binding to 25-hydroxycholesterol%2C leading to decreased interaction with SCAP. G->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26160948;Dbxref=PMID:26160948	
Q9Y5U4	UniProtKB	Mutagenesis	214	214	.	.	.	Note=Promotes ubiquitination by AMFR/gp78%2C which does not take place normally. E->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17043353;Dbxref=PMID:17043353	
Q9Y5U4	UniProtKB	Mutagenesis	215	215	.	.	.	Note=Prevents degradation because of impaired ubiquitination. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:31953408;Dbxref=PMID:31953408