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Protein

DnaJ homolog subfamily C member 15

Gene

DNAJC15

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negative regulator of the mitochondrial respiratory chain. Prevents mitochondrial hyperpolarization state and restricts mitochondrial generation of ATP (By similarity). Acts as an import component of the TIM23 translocase complex. Stimulates the ATPase activity of HSPA9.By similarity1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Protein transport, Translocation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily C member 15
Alternative name(s):
Cell growth-inhibiting gene 22 protein
Methylation-controlled J protein
Short name:
MCJ
Gene namesi
Name:DNAJC15
Synonyms:DNAJD1
ORF Names:GIG22, HSD18
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

HGNCiHGNC:20325. DNAJC15.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3535Mitochondrial intermembraneSequence analysisAdd
BLAST
Transmembranei36 – 5823HelicalSequence analysisAdd
BLAST
Topological domaini59 – 15092Mitochondrial matrixSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Involvement in diseasei

Absent or down-regulated in many advanced cases of ovarian adenocarcinoma, due to hypermethylation and allelic loss. Loss of expression correlates with increased resistance to antineoplastic drugs, such as cisplatin.

Organism-specific databases

PharmGKBiPA134932264.

Polymorphism and mutation databases

BioMutaiDNAJC15.
DMDMi110808202.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 150150DnaJ homolog subfamily C member 15PRO_0000247139Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei104 – 1041PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Y5T4.
MaxQBiQ9Y5T4.
PaxDbiQ9Y5T4.
PRIDEiQ9Y5T4.

PTM databases

iPTMnetiQ9Y5T4.
PhosphoSiteiQ9Y5T4.

Expressioni

Tissue specificityi

Expressed at highest levels in heart, followed by liver and kidney.2 Publications

Gene expression databases

BgeeiQ9Y5T4.
CleanExiHS_DNAJC15.
GenevisibleiQ9Y5T4. HS.

Organism-specific databases

HPAiHPA007988.
HPA052154.

Interactioni

Subunit structurei

Interacts with the TIM23 complex. Directly interacts with PAM16/MAGMAS; this interaction counteracts DNAJC15-dependent stimulation of HSPA9 ATPase activity. Associates with complex I of the mitochondrial electron transfer chain; this interaction may interfere with the formation of supercomplexes that facilitate the transfer of electrons between complexes (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
FAM9BQ8IZU03EBI-10329228,EBI-10175124
SIAH1Q8IUQ43EBI-10329228,EBI-747107

Protein-protein interaction databases

BioGridi118871. 7 interactions.
IntActiQ9Y5T4. 2 interactions.
STRINGi9606.ENSP00000368523.

Structurei

3D structure databases

ProteinModelPortaliQ9Y5T4.
SMRiQ9Y5T4. Positions 83-145.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini96 – 15055JPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 J domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0723. Eukaryota.
COG2214. LUCA.
GeneTreeiENSGT00390000017303.
HOGENOMiHOG000115841.
HOVERGENiHBG057483.
InParanoidiQ9Y5T4.
KOiK09535.
OMAiHRRVMIL.
OrthoDBiEOG71G9XB.
PhylomeDBiQ9Y5T4.
TreeFamiTF320584.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Y5T4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAARGVIAPV GESLRYAEYL QPSAKRPDAD VDQQRLVRSL IAVGLGVAAL
60 70 80 90 100
AFAGRYAFRI WKPLEQVITE TAKKISTPSF SSYYKGGFEQ KMSRREAGLI
110 120 130 140 150
LGVSPSAGKA KIRTAHRRVM ILNHPDKGGS PYVAAKINEA KDLLETTTKH
Length:150
Mass (Da):16,383
Last modified:July 25, 2006 - v2
Checksum:i988059B686D39967
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti34 – 341Q → R in AAP20049 (Ref. 2) Curated
Sequence conflicti131 – 1311P → S in AAP20049 (Ref. 2) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti35 – 351R → G.2 Publications
Corresponds to variant rs12015 [ dbSNP | Ensembl ].
VAR_027077

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF126743 mRNA. Translation: AAD38506.1.
AY248898 mRNA. Translation: AAP20049.1.
AY512565 mRNA. Translation: AAS80157.1.
AK311866 mRNA. Translation: BAG34807.1.
AL445217 Genomic DNA. Translation: CAI13480.1.
CH471075 Genomic DNA. Translation: EAX08688.1.
BC010910 mRNA. Translation: AAH10910.1.
BC095400 mRNA. Translation: AAH95400.1.
CCDSiCCDS9388.1.
RefSeqiNP_037370.2. NM_013238.2.
UniGeneiHs.438830.

Genome annotation databases

EnsembliENST00000379221; ENSP00000368523; ENSG00000120675.
GeneIDi29103.
KEGGihsa:29103.
UCSCiuc001uyy.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF126743 mRNA. Translation: AAD38506.1.
AY248898 mRNA. Translation: AAP20049.1.
AY512565 mRNA. Translation: AAS80157.1.
AK311866 mRNA. Translation: BAG34807.1.
AL445217 Genomic DNA. Translation: CAI13480.1.
CH471075 Genomic DNA. Translation: EAX08688.1.
BC010910 mRNA. Translation: AAH10910.1.
BC095400 mRNA. Translation: AAH95400.1.
CCDSiCCDS9388.1.
RefSeqiNP_037370.2. NM_013238.2.
UniGeneiHs.438830.

3D structure databases

ProteinModelPortaliQ9Y5T4.
SMRiQ9Y5T4. Positions 83-145.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118871. 7 interactions.
IntActiQ9Y5T4. 2 interactions.
STRINGi9606.ENSP00000368523.

PTM databases

iPTMnetiQ9Y5T4.
PhosphoSiteiQ9Y5T4.

Polymorphism and mutation databases

BioMutaiDNAJC15.
DMDMi110808202.

Proteomic databases

EPDiQ9Y5T4.
MaxQBiQ9Y5T4.
PaxDbiQ9Y5T4.
PRIDEiQ9Y5T4.

Protocols and materials databases

DNASUi29103.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000379221; ENSP00000368523; ENSG00000120675.
GeneIDi29103.
KEGGihsa:29103.
UCSCiuc001uyy.4. human.

Organism-specific databases

CTDi29103.
GeneCardsiDNAJC15.
HGNCiHGNC:20325. DNAJC15.
HPAiHPA007988.
HPA052154.
MIMi615339. gene.
neXtProtiNX_Q9Y5T4.
PharmGKBiPA134932264.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0723. Eukaryota.
COG2214. LUCA.
GeneTreeiENSGT00390000017303.
HOGENOMiHOG000115841.
HOVERGENiHBG057483.
InParanoidiQ9Y5T4.
KOiK09535.
OMAiHRRVMIL.
OrthoDBiEOG71G9XB.
PhylomeDBiQ9Y5T4.
TreeFamiTF320584.

Miscellaneous databases

ChiTaRSiDNAJC15. human.
GenomeRNAii29103.
PROiQ9Y5T4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Y5T4.
CleanExiHS_DNAJC15.
GenevisibleiQ9Y5T4. HS.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Loss of expression of a new member of the DNAJ protein family confers resistance to chemotherapeutic agents used in the treatment of ovarian cancer."
    Shridhar V., Bible K.C., Staub J., Avula R., Lee Y.K., Kalli K., Huang H., Hartmann L.C., Kaufmann S.H., Smith D.I.
    Cancer Res. 61:4258-4265(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INVOLVEMENT IN OVARIAN ADENOCARCINOMA, VARIANT GLY-35.
  2. "A new spermatogenesis-related gene."
    Yang C.B., Miao S.Y., Zhang X.D., Qiao Y., Liang G., Wang L.F.
    Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  3. "Identification of a cell growth inhibiting gene."
    Kim J.W.
    Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. "The DNA sequence and analysis of human chromosome 13."
    Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
    Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-35.
    Tissue: Skin.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Methylation-controlled J-protein MCJ acts in the import of proteins into human mitochondria."
    Schusdziarra C., Blamowska M., Azem A., Hell K.
    Hum. Mol. Genet. 22:1348-1357(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PAM16, ASSOCIATION WITH THE TIM23 COMPLEX, SUBCELLULAR LOCATION, TOPOLOGY.
  11. "MCJ/DnaJC15, an endogenous mitochondrial repressor of the respiratory chain that controls metabolic alterations."
    Hatle K.M., Gummadidala P., Navasa N., Bernardo E., Dodge J., Silverstrim B., Fortner K., Burg E., Suratt B.T., Hammer J., Radermacher M., Taatjes D.J., Thornton T., Anguita J., Rincon M.
    Mol. Cell. Biol. 33:2302-2314(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiDJC15_HUMAN
AccessioniPrimary (citable) accession number: Q9Y5T4
Secondary accession number(s): B2R4L0, Q5T219, Q6X963
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: June 8, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.