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Protein

Matrix metalloproteinase-24

Gene

MMP24

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Metalloprotease that mediates cleavage of N-cadherin (CDH2) and acts as a regulator of neuro-immune interactions and neural stem cell quiescence. Involved in cell-cell interactions between nociceptive neurites and mast cells, possibly by mediating cleavage of CDH2, thereby acting as a mediator of peripheral thermal nociception and inflammatory hyperalgesia. Key regulator of neural stem cells quiescence by mediating cleavage of CDH2, affecting CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate their quiescence. May play a role in axonal growth. Able to activate progelatinase A. May also be a proteoglycanase involved in degradation of proteoglycans, such as dermatan sulfate and chondroitin sulfate proteoglycans. Cleaves partially fibronectin, but not collagen type I, nor laminin (By similarity).By similarity

Cofactori

Protein has several cofactor binding sites:
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
  • Ca2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi139Zinc; in inhibited formBy similarity1
Metal bindingi282Zinc; catalyticPROSITE-ProRule annotation1
Active sitei283PROSITE-ProRule annotation1
Metal bindingi286Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi292Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • enzyme activator activity Source: ProtInc
  • metalloendopeptidase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000125966-MONOMER.
ReactomeiR-HSA-1592389. Activation of Matrix Metalloproteinases.

Protein family/group databases

MEROPSiM10.023.

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix metalloproteinase-24 (EC:3.4.24.-)
Short name:
MMP-24
Alternative name(s):
Membrane-type matrix metalloproteinase 5
Short name:
MT-MMP 5
Short name:
MTMMP5
Membrane-type-5 matrix metalloproteinase
Short name:
MT5-MMP
Short name:
MT5MMP
Cleaved into the following chain:
Gene namesi
Name:MMP24
Synonyms:MT5MMP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:7172. MMP24.

Subcellular locationi

Matrix metalloproteinase-24 :
Processed matrix metalloproteinase-24 :

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini53 – 602ExtracellularSequence analysisAdd BLAST550
Transmembranei603 – 623HelicalSequence analysisAdd BLAST21
Topological domaini624 – 645CytoplasmicSequence analysisAdd BLAST22

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Extracellular matrix, Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi10893.
OpenTargetsiENSG00000125966.
PharmGKBiPA30881.

Chemistry databases

ChEMBLiCHEMBL5050.
DrugBankiDB00786. Marimastat.

Polymorphism and mutation databases

BioMutaiMMP24.
DMDMi12585280.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 52Sequence analysisAdd BLAST52
PropeptideiPRO_000002884653 – 155By similarityAdd BLAST103
ChainiPRO_0000028847156 – 645Matrix metalloproteinase-24Add BLAST490
ChainiPRO_0000302758156 – 581Processed matrix metalloproteinase-24By similarityAdd BLAST426

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi380 ↔ 569By similarity

Post-translational modificationi

Cleaved by a furin endopeptidase in the trans-Golgi network.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei581 – 582Cleavage; by furinBy similarity2

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Zymogen

Proteomic databases

EPDiQ9Y5R2.
MaxQBiQ9Y5R2.
PaxDbiQ9Y5R2.
PeptideAtlasiQ9Y5R2.
PRIDEiQ9Y5R2.

PTM databases

iPTMnetiQ9Y5R2.
PhosphoSitePlusiQ9Y5R2.

Expressioni

Tissue specificityi

Predominantly expressed in brain, kidney, pancreas and lung. Overexpressed in a series of brain tumors, including astrocytomas and glioblastomas.1 Publication

Gene expression databases

BgeeiENSG00000125966.
CleanExiHS_MMP24.
GenevisibleiQ9Y5R2. HS.

Organism-specific databases

HPAiHPA049280.

Interactioni

Subunit structurei

Interacts (via PDZ-binding motif) with APBA3 (via PDZ domain). Interacts with GRIP1 and GRIP2 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ9Y5R2. 1 interactor.
MINTiMINT-7897838.
STRINGi9606.ENSP00000246186.

Chemistry databases

BindingDBiQ9Y5R2.

Structurei

3D structure databases

ProteinModelPortaliQ9Y5R2.
SMRiQ9Y5R2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati377 – 425Hemopexin 1Add BLAST49
Repeati426 – 471Hemopexin 2Add BLAST46
Repeati473 – 521Hemopexin 3Add BLAST49
Repeati522 – 569Hemopexin 4Add BLAST48

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi137 – 144Cysteine switchBy similarity8
Motifi643 – 645PDZ-binding3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi149 – 152Poly-Arg4

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
The PDZ-binding motif (also named EWV motif) is required for interaction with PDZ domains of APBA3 and recycling through the trans-Golgi network.By similarity

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated
Contains 4 hemopexin repeats.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
GeneTreeiENSGT00760000118870.
HOGENOMiHOG000217928.
HOVERGENiHBG052484.
InParanoidiQ9Y5R2.
KOiK08002.
OMAiFQFKNKA.
OrthoDBiEOG091G03DP.
PhylomeDBiQ9Y5R2.
TreeFamiTF352396.

Family and domain databases

CDDicd00094. HX. 1 hit.
cd04278. ZnMc_MMP. 1 hit.
Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR033739. M10A_MMP.
IPR024079. MetalloPept_cat_dom.
IPR028723. MMP24.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR021805. Pept_M10A_metallopeptidase_C.
IPR016293. Pept_M10A_stromelysin-type.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PANTHERiPTHR10201:SF138. PTHR10201:SF138. 2 hits.
PfamiPF11857. DUF3377. 1 hit.
PF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Y5R2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRSRGGRAA PGPPPPPPPP GQAPRWSRWR VPGRLLLLLL PALCCLPGAA
60 70 80 90 100
RAAAAAAGAG NRAAVAVAVA RADEAEAPFA GQNWLKSYGY LLPYDSRASA
110 120 130 140 150
LHSAKALQSA VSTMQQFYGI PVTGVLDQTT IEWMKKPRCG VPDHPHLSRR
160 170 180 190 200
RRNKRYALTG QKWRQKHITY SIHNYTPKVG ELDTRKAIRQ AFDVWQKVTP
210 220 230 240 250
LTFEEVPYHE IKSDRKEADI MIFFASGFHG DSSPFDGEGG FLAHAYFPGP
260 270 280 290 300
GIGGDTHFDS DEPWTLGNAN HDGNDLFLVA VHELGHALGL EHSSDPSAIM
310 320 330 340 350
APFYQYMETH NFKLPQDDLQ GIQKIYGPPA EPLEPTRPLP TLPVRRIHSP
360 370 380 390 400
SERKHERQPR PPRPPLGDRP STPGTKPNIC DGNFNTVALF RGEMFVFKDR
410 420 430 440 450
WFWRLRNNRV QEGYPMQIEQ FWKGLPARID AAYERADGRF VFFKGDKYWV
460 470 480 490 500
FKEVTVEPGY PHSLGELGSC LPREGIDTAL RWEPVGKTYF FKGERYWRYS
510 520 530 540 550
EERRATDPGY PKPITVWKGI PQAPQGAFIS KEGYYTYFYK GRDYWKFDNQ
560 570 580 590 600
KLSVEPGYPR NILRDWMGCN QKEVERRKER RLPQDDVDIM VTINDVPGSV
610 620 630 640
NAVAVVIPCI LSLCILVLVY TIFQFKNKTG PQPVTYYKRP VQEWV
Length:645
Mass (Da):73,231
Last modified:November 1, 1999 - v1
Checksum:i06B2B76EA3DABB9D
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_060166564R → H.Corresponds to variant rs751887dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF131284 mRNA. Translation: AAD42962.1.
AB021227 mRNA. Translation: BAA82967.1.
AL121753 Genomic DNA. Translation: CAX12722.1.
CCDSiCCDS46593.1.
RefSeqiNP_006681.1. NM_006690.3.
UniGeneiHs.715494.

Genome annotation databases

EnsembliENST00000246186; ENSP00000246186; ENSG00000125966.
GeneIDi10893.
KEGGihsa:10893.
UCSCiuc002xbu.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF131284 mRNA. Translation: AAD42962.1.
AB021227 mRNA. Translation: BAA82967.1.
AL121753 Genomic DNA. Translation: CAX12722.1.
CCDSiCCDS46593.1.
RefSeqiNP_006681.1. NM_006690.3.
UniGeneiHs.715494.

3D structure databases

ProteinModelPortaliQ9Y5R2.
SMRiQ9Y5R2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9Y5R2. 1 interactor.
MINTiMINT-7897838.
STRINGi9606.ENSP00000246186.

Chemistry databases

BindingDBiQ9Y5R2.
ChEMBLiCHEMBL5050.
DrugBankiDB00786. Marimastat.

Protein family/group databases

MEROPSiM10.023.

PTM databases

iPTMnetiQ9Y5R2.
PhosphoSitePlusiQ9Y5R2.

Polymorphism and mutation databases

BioMutaiMMP24.
DMDMi12585280.

Proteomic databases

EPDiQ9Y5R2.
MaxQBiQ9Y5R2.
PaxDbiQ9Y5R2.
PeptideAtlasiQ9Y5R2.
PRIDEiQ9Y5R2.

Protocols and materials databases

DNASUi10893.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000246186; ENSP00000246186; ENSG00000125966.
GeneIDi10893.
KEGGihsa:10893.
UCSCiuc002xbu.3. human.

Organism-specific databases

CTDi10893.
DisGeNETi10893.
GeneCardsiMMP24.
HGNCiHGNC:7172. MMP24.
HPAiHPA049280.
MIMi604871. gene.
neXtProtiNX_Q9Y5R2.
OpenTargetsiENSG00000125966.
PharmGKBiPA30881.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
GeneTreeiENSGT00760000118870.
HOGENOMiHOG000217928.
HOVERGENiHBG052484.
InParanoidiQ9Y5R2.
KOiK08002.
OMAiFQFKNKA.
OrthoDBiEOG091G03DP.
PhylomeDBiQ9Y5R2.
TreeFamiTF352396.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000125966-MONOMER.
ReactomeiR-HSA-1592389. Activation of Matrix Metalloproteinases.

Miscellaneous databases

GeneWikiiMMP24.
GenomeRNAii10893.
PROiQ9Y5R2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000125966.
CleanExiHS_MMP24.
GenevisibleiQ9Y5R2. HS.

Family and domain databases

CDDicd00094. HX. 1 hit.
cd04278. ZnMc_MMP. 1 hit.
Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR033739. M10A_MMP.
IPR024079. MetalloPept_cat_dom.
IPR028723. MMP24.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR021805. Pept_M10A_metallopeptidase_C.
IPR016293. Pept_M10A_stromelysin-type.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PANTHERiPTHR10201:SF138. PTHR10201:SF138. 2 hits.
PfamiPF11857. DUF3377. 1 hit.
PF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMMP24_HUMAN
AccessioniPrimary (citable) accession number: Q9Y5R2
Secondary accession number(s): B7ZBG8, Q9H440
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 156 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.