Q9Y5Q9 (TF3C3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
General transcription factor 3C polypeptide 3

Alternative name(s):
Transcription factor IIIC 102 kDa subunit
Short name=TFIIIC 102 kDa subunit
Short name=TFIIIC102
Transcription factor IIIC subunit gamma
Short name=TF3C-gamma

Gene names

Name:

GTF3C3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	886 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in RNA polymerase III-mediated transcription. Integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex that directly binds tRNA and virus-associated RNA promoters.
Subunit structure	Part of the TFIIIC subcomplex TFIIIC2, consisting of six subunits, GTF3C1, GTF3C2, GTF3C3, GTF3C4, GTF3C5 and GTF3C6. Interacts with BRF1 and TBP. Ref.1
Subcellular location	Nucleus.
Sequence similarities	Contains 11 TPR repeats.
Sequence caution	The sequence BAB15638.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat TPR repeat
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	5S class rRNA transcription from RNA polymerase III type 1 promoter Inferred by curator PubMed 17409385. Source: HGNC tRNA transcription from RNA polymerase III promoter Inferred by curator PubMed 17409385. Source: HGNC
Cellular_component	transcription factor TFIIIC complex Inferred from direct assay PubMed 17409385. Source: HGNC
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y5Q9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y5Q9-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     407-413: PLLTTLV → VCNKICT
     414-886: Missing.

Note: May be due to exon skipping.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 886	886	General transcription factor 3C polypeptide 3		PRO_0000106363
Regions
	Repeat	149 – 182	34	TPR 1
	Repeat	183 – 216	34	TPR 2
	Repeat	217 – 250	34	TPR 3
	Repeat	252 – 284	33	TPR 4
	Repeat	290 – 323	34	TPR 5
	Repeat	326 – 361	36	TPR 6
	Repeat	421 – 454	34	TPR 7
	Repeat	456 – 489	34	TPR 8
	Repeat	491 – 523	33	TPR 9
	Repeat	733 – 766	34	TPR 10
	Repeat	811 – 844	34	TPR 11
	Compositional bias	92 – 114	23	Glu-rich
Amino acid modifications
	Modified residue	43	1	Phosphoserine Ref.8 Ref.9 Ref.11
Natural variations
	Alternative sequence	407 – 413	7	PLLTTLV → VCNKICT in isoform 2.		VSP_010353
	Alternative sequence	414 – 886	473	Missing in isoform 2.		VSP_010354
	Natural variant	70	1	N → S. Ref.6 Corresponds to variant rs11559078 [ dbSNP | Ensembl ].		VAR_061902
Experimental info
	Sequence conflict	412 – 413	2	LV → HL in AAH15995. Ref.6
	Sequence conflict	589	1	I → M in BAB15638. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 9B97269929F9765F	FASTA	886	101,272
	10 20 30 40 50 60 MSGFSPELID YLEGKISFEE FERRREERKT REKKSLQEKG KLSAEENPDD SEVPSSSGIN 70 80 90 100 110 120 STKSQDKDVN EGETSDGVRK SVHKVFASML GENEDDEEEE EEEEEEEEEE ETPEQPTAGD 130 140 150 160 170 180 VFVLEMVLNR ETKKMMKEKR PRSKLPRALR GLMGEANIRF ARGEREEAIL MCMEIIRQAP 190 200 210 220 230 240 LAYEPFSTLA MIYEDQGDME KSLQFELIAA HLNPSDTEEW VRLAEMSLEQ DNIKQAIFCY 250 260 270 280 290 300 TKALKYEPTN VRYLWERSSL YEQMGDHKMA MDGYRRILNL LSPSDGERFM QLARDMAKSY 310 320 330 340 350 360 YEANDVTSAI NIIDEAFSKH QGLVSMEDVN IAAELYISNK QYDKALEIIT DFSGIVLEKK 370 380 390 400 410 420 TSEEGTSEEN KAPENVTCTI PDGVPIDITV KLMVCLVHLN ILEPLNPLLT TLVEQNPEDM 430 440 450 460 470 480 GDLYLDVAEA FLDVGEYNSA LPLLSALVCS ERYNLAVVWL RHAECLKALG YMERAAESYG 490 500 510 520 530 540 KVVDLAPLHL DARISLSTLQ QQLGQPEKAL EALEPMYDPD TLAQDANAAQ QELKLLLHRS 550 560 570 580 590 600 TLLFSQGKMY GYVDTLLTML AMLLKVAMNR AQVCLISSSK SGERHLYLIK VSRDKISDSN 610 620 630 640 650 660 DQESANCDAK AIFAVLTSVL TKDDWWNLLL KAIYSLCDLS RFQEAELLVD SSLEYYSFYD 670 680 690 700 710 720 DRQKRKELEY FGLSAAILDK NFRKAYNYIR IMVMENVNKP QLWNIFNQVT MHSQDVRHHR 730 740 750 760 770 780 FCLRLMLKNP ENHALCVLNG HNAFVSGSFK HALGQYVQAF RTHPDEPLYS FCIGLTFIHM 790 800 810 820 830 840 ASQKYVLRRH ALIVQGFSFL NRYLSLRGPC QESFYNLGRG LHQLGLIHLA IHYYQKALEL 850 860 870 880 PPLVVEGIEL DQLDLRRDIA YNLSLIYQSS GNTGMAQTLL YTYCSI
	Isoform 2 (Short) [UniParc]. Checksum: A1628BC6E182DDCE	FASTA	413	47,258
	10 20 30 40 50 60 MSGFSPELID YLEGKISFEE FERRREERKT REKKSLQEKG KLSAEENPDD SEVPSSSGIN 70 80 90 100 110 120 STKSQDKDVN EGETSDGVRK SVHKVFASML GENEDDEEEE EEEEEEEEEE ETPEQPTAGD 130 140 150 160 170 180 VFVLEMVLNR ETKKMMKEKR PRSKLPRALR GLMGEANIRF ARGEREEAIL MCMEIIRQAP 190 200 210 220 230 240 LAYEPFSTLA MIYEDQGDME KSLQFELIAA HLNPSDTEEW VRLAEMSLEQ DNIKQAIFCY 250 260 270 280 290 300 TKALKYEPTN VRYLWERSSL YEQMGDHKMA MDGYRRILNL LSPSDGERFM QLARDMAKSY 310 320 330 340 350 360 YEANDVTSAI NIIDEAFSKH QGLVSMEDVN IAAELYISNK QYDKALEIIT DFSGIVLEKK 370 380 390 400 410 TSEEGTSEEN KAPENVTCTI PDGVPIDITV KLMVCLVHLN ILEPLNVCNK ICT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of two evolutionarily conserved subunits (TFIIIC102 and TFIIIC63) of human TFIIIC and their involvement in functional interactions with TFIIIB and RNA polymerase III." Hsieh Y.-J., Wang Z., Kovelman R., Roeder R.G. Mol. Cell. Biol. 19:4944-4952(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH BRF1 AND TBP, IDENTIFICATION OF SUBUNITS OF TFIIIC2 COMPLEX.
[2]	"Identification of a short isoform of hTFIIIC102 subunit." Hegde R., Alnemri E. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Hepatoma and Placenta.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT SER-70. Tissue: Bone marrow and Lymph.
[7]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 166-886 (ISOFORM 1). Tissue: Amygdala.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF133123 mRNA. Translation: AAD41475.1. AF465407 mRNA. Translation: AAL73493.1. AK027050 mRNA. Translation: BAB15638.1. Different initiation. AK314718 mRNA. Translation: BAG37262.1. AC012486 Genomic DNA. Translation: AAX88853.1. CH471063 Genomic DNA. Translation: EAW70132.1. BC015995 mRNA. Translation: AAH15995.1. BC043347 mRNA. Translation: AAH43347.1. AL137453 mRNA. Translation: CAB70745.1.
IPI	IPI00015806. IPI00411594.
PIR	T46268.
RefSeq	NP_001193703.1. NM_001206774.1. NP_036218.1. NM_012086.4.
UniGene	Hs.470996. Hs.737375.
3D structure databases
ProteinModelPortal	Q9Y5Q9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y5Q9. 9 interactions.
STRING	9606.ENSP00000263956.
PTM databases
PhosphoSite	Q9Y5Q9.
Polymorphism databases
DMDM	47606223.
Proteomic databases
PaxDb	Q9Y5Q9.
PeptideAtlas	Q9Y5Q9.
PRIDE	Q9Y5Q9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000263956; ENSP00000263956; ENSG00000119041. ENST00000409364; ENSP00000386465; ENSG00000119041.
GeneID	9330.
KEGG	hsa:9330.
UCSC	uc002uts.3. human. uc002utu.3. human.
Organism-specific databases
CTD	9330.
GeneCards	GC02M197592.
HGNC	HGNC:4666. GTF3C3.
MIM	604888. gene.
neXtProt	NX_Q9Y5Q9.
PharmGKB	PA29054.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG275814.
HOVERGEN	HBG057124.
InParanoid	Q9Y5Q9.
KO	K15201.
OMA	IHLAIHY.
OrthoDB	EOG4CJVGH.
PhylomeDB	Q9Y5Q9.
Enzyme and pathway databases
Reactome	REACT_1788. Transcription. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	Q9Y5Q9.
Bgee	Q9Y5Q9.
CleanEx	HS_GTF3C3.
Genevestigator	Q9Y5Q9.
GermOnline	ENSG00000119041. Homo sapiens.
Family and domain databases
Gene3D	1.25.40.10. 3 hits.
InterPro	IPR001440. TPR-1. IPR013026. TPR-contain_dom. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
Pfam	PF00515. TPR_1. 1 hit. PF13181. TPR_8. 1 hit. [Graphical view]
SMART	SM00028. TPR. 5 hits. [Graphical view]
PROSITE	PS50005. TPR. 6 hits. PS50293. TPR_REGION. 3 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	9330.
NextBio	34947.
SOURCE	Search...

Entry information

Entry name

TF3C3_HUMAN

Accession

Primary (citable) accession number: Q9Y5Q9
Secondary accession number(s): Q4ZG48 , Q86XJ8, Q8WX84, Q96B44, Q9H5I8, Q9NT97

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.