Q9Y5Q8 (TF3C5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
General transcription factor 3C polypeptide 5

Alternative name(s):
TF3C-epsilon
Transcription factor IIIC 63 kDa subunit
Short name=TFIIIC 63 kDa subunit
Short name=TFIIIC63
Transcription factor IIIC subunit epsilon

Gene names

Name:	GTF3C5
ORF Names:	CDABP0017

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in RNA polymerase III-mediated transcription. Integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex that directly binds tRNA and virus-associated RNA promoters.
Subunit structure	Part of the TFIIIC subcomplex TFIIIC2, consisting of six subunits, GTF3C1, GTF3C2, GTF3C3, GTF3C4, GTF3C5 and GTF3C6. Interacts with BRF1, GTF3C6 and TBP. Ref.1 Ref.7
Subcellular location	Nucleus.
Sequence similarities	Belongs to the TFIIIC subunit 5 family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	5S class rRNA transcription from RNA polymerase III type 1 promoter Inferred by curator Ref.7. Source: HGNC tRNA transcription from RNA polymerase III promoter Inferred by curator Ref.7. Source: HGNC
Cellular_component	transcription factor TFIIIC complex Inferred from direct assay Ref.7. Source: HGNC
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y5Q8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y5Q8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     56-65: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9Y5Q8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     389-389: K → KVSLQTLR

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 519	519	General transcription factor 3C polypeptide 5		PRO_0000209715
Regions
	Compositional bias	478 – 514	37	Glu-rich
	Compositional bias	486 – 499	14	Poly-Glu
Natural variations
	Alternative sequence	56 – 65	10	Missing in isoform 2.		VSP_010355
	Alternative sequence	389	1	K → KVSLQTLR in isoform 3.		VSP_035196
	Natural variant	445	1	D → N. Corresponds to variant rs637435 [ dbSNP | Ensembl ].		VAR_053727
Experimental info
	Sequence conflict	372	1	P → R in AAD41476. Ref.1
	Sequence conflict	375	1	T → A in BAC04993. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 24, 2004. Version 2. Checksum: 088B8B6125CEEF36	FASTA	519	59,571
	10 20 30 40 50 60 MAAEAADLGL GAAVPVELRR ERRMVCVEYP GVVRDVAKML PTLGGEEGVS RIYADPTKRL 70 80 90 100 110 120 ELYFRPKDPY CHPVCANRFS TSSLLLRIRK RTRRQKGVLG TEAHSEVTFD MEILGIISTI 130 140 150 160 170 180 YKFQGMSDFQ YLAVHTEAGG KHTSMYDKVL MLRPEKEAFF HQELPLYIPP PIFSRLDAPV 190 200 210 220 230 240 DYFYRPETQH REGYNNPPIS GENLIGLSRA RRPHNAIFVN FEDEEVPKQP LEAAAQTWRR 250 260 270 280 290 300 VCTNPVDRKV EEELRKLFDI RPIWSRNAVK ANISVHPDKL KVLLPFIAYY MITGPWRSLW 310 320 330 340 350 360 IRFGYDPRKN PDAKIYQVLD FRIRCGMKHG YAPSDLPVKA KRSTYNYSLP ITVKKTSSQL 370 380 390 400 410 420 VTMHDLKQGL GPSGTSGARK PASSKYKLKD SVYIFREGAL PPYRQMFYQL CDLNVEELQK 430 440 450 460 470 480 IIHRNDGAEN SCTERDGWCL PKTSDELRDT MSLMIRQTIR SKRPALFSSS AKADGGKEQL 490 500 510 TYESGEDEED EEEEEEEEED FKPSDGSENE METEILDYV
	Isoform 2 [UniParc]. Checksum: 58222432D6D3A333	FASTA	509	58,266
	10 20 30 40 50 60 MAAEAADLGL GAAVPVELRR ERRMVCVEYP GVVRDVAKML PTLGGEEGVS RIYADPKDPY 70 80 90 100 110 120 CHPVCANRFS TSSLLLRIRK RTRRQKGVLG TEAHSEVTFD MEILGIISTI YKFQGMSDFQ 130 140 150 160 170 180 YLAVHTEAGG KHTSMYDKVL MLRPEKEAFF HQELPLYIPP PIFSRLDAPV DYFYRPETQH 190 200 210 220 230 240 REGYNNPPIS GENLIGLSRA RRPHNAIFVN FEDEEVPKQP LEAAAQTWRR VCTNPVDRKV 250 260 270 280 290 300 EEELRKLFDI RPIWSRNAVK ANISVHPDKL KVLLPFIAYY MITGPWRSLW IRFGYDPRKN 310 320 330 340 350 360 PDAKIYQVLD FRIRCGMKHG YAPSDLPVKA KRSTYNYSLP ITVKKTSSQL VTMHDLKQGL 370 380 390 400 410 420 GPSGTSGARK PASSKYKLKD SVYIFREGAL PPYRQMFYQL CDLNVEELQK IIHRNDGAEN 430 440 450 460 470 480 SCTERDGWCL PKTSDELRDT MSLMIRQTIR SKRPALFSSS AKADGGKEQL TYESGEDEED 490 500 EEEEEEEEED FKPSDGSENE METEILDYV
	Isoform 3 [UniParc]. Checksum: FAA2A99DF60A0718	FASTA	526	60,369
	10 20 30 40 50 60 MAAEAADLGL GAAVPVELRR ERRMVCVEYP GVVRDVAKML PTLGGEEGVS RIYADPTKRL 70 80 90 100 110 120 ELYFRPKDPY CHPVCANRFS TSSLLLRIRK RTRRQKGVLG TEAHSEVTFD MEILGIISTI 130 140 150 160 170 180 YKFQGMSDFQ YLAVHTEAGG KHTSMYDKVL MLRPEKEAFF HQELPLYIPP PIFSRLDAPV 190 200 210 220 230 240 DYFYRPETQH REGYNNPPIS GENLIGLSRA RRPHNAIFVN FEDEEVPKQP LEAAAQTWRR 250 260 270 280 290 300 VCTNPVDRKV EEELRKLFDI RPIWSRNAVK ANISVHPDKL KVLLPFIAYY MITGPWRSLW 310 320 330 340 350 360 IRFGYDPRKN PDAKIYQVLD FRIRCGMKHG YAPSDLPVKA KRSTYNYSLP ITVKKTSSQL 370 380 390 400 410 420 VTMHDLKQGL GPSGTSGARK PASSKYKLKV SLQTLRDSVY IFREGALPPY RQMFYQLCDL 430 440 450 460 470 480 NVEELQKIIH RNDGAENSCT ERDGWCLPKT SDELRDTMSL MIRQTIRSKR PALFSSSAKA 490 500 510 520 DGGKEQLTYE SGEDEEDEEE EEEEEEDFKP SDGSENEMET EILDYV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of two evolutionarily conserved subunits (TFIIIC102 and TFIIIC63) of human TFIIIC and their involvement in functional interactions with TFIIIB and RNA polymerase III." Hsieh Y.-J., Wang Z., Kovelman R., Roeder R.G. Mol. Cell. Biol. 19:4944-4952(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH BRF1 AND TBP, IDENTIFICATION OF SUBUNITS OF TFIIIC2 COMPLEX.
[2]	"Pediatric leukemia cDNA sequencing project." Zhou J., Yu W., Tang H., Mei G., Tsang Y.T.M., Bouck J., Gibbs R.A., Margolin J.F. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Leukemia.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain and Spleen.
[4]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain, Kidney, Ovary and Skin.
[7]	"Identification, molecular cloning, and characterization of the sixth subunit of human transcription factor TFIIIC." Dumay-Odelot H., Marck C., Durrieu-Gaillard S., Lefebvre O., Jourdain S., Prochazkova M., Pflieger A., Teichmann M. J. Biol. Chem. 282:17179-17189(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN TFIIIC COMPLEX, INTERACTION WITH GTF3C6.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF133124 mRNA. Translation: AAD41476.1. AY007123 mRNA. Translation: AAG01991.1. AK055092 mRNA. Translation: BAG51466.1. AK097295 mRNA. Translation: BAC04993.1. AL162417 Genomic DNA. Translation: CAI13406.1. AL162417 Genomic DNA. Translation: CAI13407.1. CH471090 Genomic DNA. Translation: EAW88026.1. CH471090 Genomic DNA. Translation: EAW88029.1. BC009741 mRNA. Translation: AAH09741.2. BC011355 mRNA. Translation: AAH11355.1. BC017337 mRNA. Translation: AAH17337.1. BC030157 mRNA. Translation: AAH30157.2.
IPI	IPI00411531. IPI00844182. IPI00879210.
RefSeq	NP_001116295.1. NM_001122823.1. NP_036219.2. NM_012087.3.
UniGene	Hs.495417.
3D structure databases
ProteinModelPortal	Q9Y5Q8.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-50823N.
IntAct	Q9Y5Q8. 3 interactions.
PTM databases
PhosphoSite	Q9Y5Q8.
Polymorphism databases
DMDM	47606222.
Proteomic databases
PaxDb	Q9Y5Q8.
PRIDE	Q9Y5Q8.
Protocols and materials databases
DNASU	9328.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000372097; ENSP00000361169; ENSG00000148308. ENST00000372108; ENSP00000361180; ENSG00000148308.
GeneID	9328.
KEGG	hsa:9328.
UCSC	uc004cci.4. human. uc004ccj.4. human.
Organism-specific databases
CTD	9328.
GeneCards	GC09P135906.
HGNC	HGNC:4668. GTF3C5.
MIM	604890. gene.
neXtProt	NX_Q9Y5Q8.
PharmGKB	PA29056.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG311463.
HOVERGEN	HBG058445.
KO	K15202.
OMA	PNDMPVK.
OrthoDB	EOG4VDPZX.
PhylomeDB	Q9Y5Q8.
Enzyme and pathway databases
Reactome	REACT_1788. Transcription. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	Q9Y5Q8.
Bgee	Q9Y5Q8.
Genevestigator	Q9Y5Q8.
GermOnline	ENSG00000148308. Homo sapiens.
Family and domain databases
InterPro	IPR019136. TF_IIIC_su-5. [Graphical view]
Pfam	PF09734. Tau95. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	GTF3C5. human.
GenomeRNAi	9328.
NextBio	34939.
SOURCE	Search...

Entry information

Entry name

TF3C5_HUMAN

Accession

Primary (citable) accession number: Q9Y5Q8
Secondary accession number(s): A6NI44 , A6NJB9, Q5T7U2, Q5T7U3, Q8N2U7, Q8N857, Q96GD9, Q9H4P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	May 24, 2004
Last modified:	April 3, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.