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Protein

General transcription factor 3C polypeptide 5

Gene

GTF3C5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in RNA polymerase III-mediated transcription. Integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex that directly binds tRNA and virus-associated RNA promoters.

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. 5S class rRNA transcription from RNA polymerase III type 1 promoter Source: HGNC
  2. gene expression Source: Reactome
  3. skeletal muscle cell differentiation Source: Ensembl
  4. transcription, DNA-templated Source: HGNC
  5. transcription from RNA polymerase III promoter Source: HGNC
  6. tRNA transcription from RNA polymerase III promoter Source: HGNC
Complete GO annotation...

Keywords - Biological processi

Transcription

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_1036. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
REACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
REACT_347. RNA Polymerase III Transcription Initiation From Type 1 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor 3C polypeptide 5
Alternative name(s):
TF3C-epsilon
Transcription factor IIIC 63 kDa subunit
Short name:
TFIIIC 63 kDa subunit
Short name:
TFIIIC63
Transcription factor IIIC subunit epsilon
Gene namesi
Name:GTF3C5
ORF Names:CDABP0017
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 9

Organism-specific databases

HGNCiHGNC:4668. GTF3C5.

Subcellular locationi

GO - Cellular componenti

  1. nucleoplasm Source: Reactome
  2. transcription factor TFIIIC complex Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29056.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 519518General transcription factor 3C polypeptide 5PRO_0000209715Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9Y5Q8.
PaxDbiQ9Y5Q8.
PRIDEiQ9Y5Q8.

PTM databases

PhosphoSiteiQ9Y5Q8.

Expressioni

Gene expression databases

BgeeiQ9Y5Q8.
ExpressionAtlasiQ9Y5Q8. baseline and differential.
GenevestigatoriQ9Y5Q8.

Interactioni

Subunit structurei

Part of the TFIIIC subcomplex TFIIIC2, consisting of six subunits, GTF3C1, GTF3C2, GTF3C3, GTF3C4, GTF3C5 and GTF3C6. Interacts with BRF1, GTF3C6 and TBP.2 Publications

Protein-protein interaction databases

BioGridi114737. 29 interactions.
DIPiDIP-50823N.
IntActiQ9Y5Q8. 4 interactions.
MINTiMINT-4777347.

Structurei

3D structure databases

ProteinModelPortaliQ9Y5Q8.
SMRiQ9Y5Q8. Positions 248-329.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi478 – 51437Glu-richAdd
BLAST
Compositional biasi486 – 49914Poly-GluAdd
BLAST

Sequence similaritiesi

Belongs to the TFIIIC subunit 5 family.Curated

Phylogenomic databases

eggNOGiNOG311463.
GeneTreeiENSGT00390000004458.
HOVERGENiHBG058445.
InParanoidiQ9Y5Q8.
KOiK15202.
OMAiLWIRYGY.
OrthoDBiEOG780RMW.
PhylomeDBiQ9Y5Q8.
TreeFamiTF313581.

Family and domain databases

InterProiIPR019136. TF_IIIC_su-5.
[Graphical view]
PfamiPF09734. Tau95. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9Y5Q8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAEAADLGL GAAVPVELRR ERRMVCVEYP GVVRDVAKML PTLGGEEGVS
60 70 80 90 100
RIYADPTKRL ELYFRPKDPY CHPVCANRFS TSSLLLRIRK RTRRQKGVLG
110 120 130 140 150
TEAHSEVTFD MEILGIISTI YKFQGMSDFQ YLAVHTEAGG KHTSMYDKVL
160 170 180 190 200
MLRPEKEAFF HQELPLYIPP PIFSRLDAPV DYFYRPETQH REGYNNPPIS
210 220 230 240 250
GENLIGLSRA RRPHNAIFVN FEDEEVPKQP LEAAAQTWRR VCTNPVDRKV
260 270 280 290 300
EEELRKLFDI RPIWSRNAVK ANISVHPDKL KVLLPFIAYY MITGPWRSLW
310 320 330 340 350
IRFGYDPRKN PDAKIYQVLD FRIRCGMKHG YAPSDLPVKA KRSTYNYSLP
360 370 380 390 400
ITVKKTSSQL VTMHDLKQGL GPSGTSGARK PASSKYKLKD SVYIFREGAL
410 420 430 440 450
PPYRQMFYQL CDLNVEELQK IIHRNDGAEN SCTERDGWCL PKTSDELRDT
460 470 480 490 500
MSLMIRQTIR SKRPALFSSS AKADGGKEQL TYESGEDEED EEEEEEEEED
510
FKPSDGSENE METEILDYV
Length:519
Mass (Da):59,571
Last modified:May 24, 2004 - v2
Checksum:i088B8B6125CEEF36
GO
Isoform 2 (identifier: Q9Y5Q8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     56-65: Missing.

Note: No experimental confirmation available.

Show »
Length:509
Mass (Da):58,266
Checksum:i58222432D6D3A333
GO
Isoform 3 (identifier: Q9Y5Q8-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     389-389: K → KVSLQTLR

Note: No experimental confirmation available.

Show »
Length:526
Mass (Da):60,369
Checksum:iFAA2A99DF60A0718
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti372 – 3721P → R in AAD41476. (PubMed:10373544)Curated
Sequence conflicti375 – 3751T → A in BAC04993. (PubMed:14702039)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti445 – 4451D → N.
Corresponds to variant rs637435 [ dbSNP | Ensembl ].
VAR_053727

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei56 – 6510Missing in isoform 2. 1 PublicationVSP_010355
Alternative sequencei389 – 3891K → KVSLQTLR in isoform 3. CuratedVSP_035196

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF133124 mRNA. Translation: AAD41476.1.
AY007123 mRNA. Translation: AAG01991.1.
AK055092 mRNA. Translation: BAG51466.1.
AK097295 mRNA. Translation: BAC04993.1.
AL162417 Genomic DNA. Translation: CAI13406.1.
AL162417 Genomic DNA. Translation: CAI13407.1.
CH471090 Genomic DNA. Translation: EAW88026.1.
CH471090 Genomic DNA. Translation: EAW88029.1.
BC009741 mRNA. Translation: AAH09741.2.
BC011355 mRNA. Translation: AAH11355.1.
BC017337 mRNA. Translation: AAH17337.1.
BC030157 mRNA. Translation: AAH30157.2.
CCDSiCCDS48050.1. [Q9Y5Q8-3]
CCDS6958.1. [Q9Y5Q8-1]
RefSeqiNP_001116295.1. NM_001122823.1. [Q9Y5Q8-3]
NP_001273638.1. NM_001286709.1.
NP_036219.2. NM_012087.3. [Q9Y5Q8-1]
UniGeneiHs.495417.

Genome annotation databases

EnsembliENST00000372097; ENSP00000361169; ENSG00000148308. [Q9Y5Q8-1]
ENST00000372108; ENSP00000361180; ENSG00000148308. [Q9Y5Q8-3]
GeneIDi9328.
KEGGihsa:9328.
UCSCiuc004cci.4. human. [Q9Y5Q8-1]
uc004ccj.4. human. [Q9Y5Q8-3]

Polymorphism databases

DMDMi47606222.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF133124 mRNA. Translation: AAD41476.1.
AY007123 mRNA. Translation: AAG01991.1.
AK055092 mRNA. Translation: BAG51466.1.
AK097295 mRNA. Translation: BAC04993.1.
AL162417 Genomic DNA. Translation: CAI13406.1.
AL162417 Genomic DNA. Translation: CAI13407.1.
CH471090 Genomic DNA. Translation: EAW88026.1.
CH471090 Genomic DNA. Translation: EAW88029.1.
BC009741 mRNA. Translation: AAH09741.2.
BC011355 mRNA. Translation: AAH11355.1.
BC017337 mRNA. Translation: AAH17337.1.
BC030157 mRNA. Translation: AAH30157.2.
CCDSiCCDS48050.1. [Q9Y5Q8-3]
CCDS6958.1. [Q9Y5Q8-1]
RefSeqiNP_001116295.1. NM_001122823.1. [Q9Y5Q8-3]
NP_001273638.1. NM_001286709.1.
NP_036219.2. NM_012087.3. [Q9Y5Q8-1]
UniGeneiHs.495417.

3D structure databases

ProteinModelPortaliQ9Y5Q8.
SMRiQ9Y5Q8. Positions 248-329.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114737. 29 interactions.
DIPiDIP-50823N.
IntActiQ9Y5Q8. 4 interactions.
MINTiMINT-4777347.

PTM databases

PhosphoSiteiQ9Y5Q8.

Polymorphism databases

DMDMi47606222.

Proteomic databases

MaxQBiQ9Y5Q8.
PaxDbiQ9Y5Q8.
PRIDEiQ9Y5Q8.

Protocols and materials databases

DNASUi9328.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000372097; ENSP00000361169; ENSG00000148308. [Q9Y5Q8-1]
ENST00000372108; ENSP00000361180; ENSG00000148308. [Q9Y5Q8-3]
GeneIDi9328.
KEGGihsa:9328.
UCSCiuc004cci.4. human. [Q9Y5Q8-1]
uc004ccj.4. human. [Q9Y5Q8-3]

Organism-specific databases

CTDi9328.
GeneCardsiGC09P135906.
HGNCiHGNC:4668. GTF3C5.
MIMi604890. gene.
neXtProtiNX_Q9Y5Q8.
PharmGKBiPA29056.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG311463.
GeneTreeiENSGT00390000004458.
HOVERGENiHBG058445.
InParanoidiQ9Y5Q8.
KOiK15202.
OMAiLWIRYGY.
OrthoDBiEOG780RMW.
PhylomeDBiQ9Y5Q8.
TreeFamiTF313581.

Enzyme and pathway databases

ReactomeiREACT_1036. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
REACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
REACT_347. RNA Polymerase III Transcription Initiation From Type 1 Promoter.

Miscellaneous databases

ChiTaRSiGTF3C5. human.
GeneWikiiGTF3C5.
GenomeRNAii9328.
NextBioi34939.
PROiQ9Y5Q8.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Y5Q8.
ExpressionAtlasiQ9Y5Q8. baseline and differential.
GenevestigatoriQ9Y5Q8.

Family and domain databases

InterProiIPR019136. TF_IIIC_su-5.
[Graphical view]
PfamiPF09734. Tau95. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of two evolutionarily conserved subunits (TFIIIC102 and TFIIIC63) of human TFIIIC and their involvement in functional interactions with TFIIIB and RNA polymerase III."
    Hsieh Y.-J., Wang Z., Kovelman R., Roeder R.G.
    Mol. Cell. Biol. 19:4944-4952(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH BRF1 AND TBP, IDENTIFICATION OF SUBUNITS OF TFIIIC2 COMPLEX.
  2. "Pediatric leukemia cDNA sequencing project."
    Zhou J., Yu W., Tang H., Mei G., Tsang Y.T.M., Bouck J., Gibbs R.A., Margolin J.F.
    Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Leukemia.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain and Spleen.
  4. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain, Kidney, Ovary and Skin.
  7. "Identification, molecular cloning, and characterization of the sixth subunit of human transcription factor TFIIIC."
    Dumay-Odelot H., Marck C., Durrieu-Gaillard S., Lefebvre O., Jourdain S., Prochazkova M., Pflieger A., Teichmann M.
    J. Biol. Chem. 282:17179-17189(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN TFIIIC COMPLEX, INTERACTION WITH GTF3C6.
  8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTF3C5_HUMAN
AccessioniPrimary (citable) accession number: Q9Y5Q8
Secondary accession number(s): A6NI44
, A6NJB9, Q5T7U2, Q5T7U3, Q8N2U7, Q8N857, Q96GD9, Q9H4P2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: February 4, 2015
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.