Q9Y5P8 (P2R3B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta

Alternative name(s):
PP2A subunit B isoform PR48
Protein phosphatase 2A 48 kDa regulatory subunit

Gene names

Name:	PPP2R3B
Synonyms:	PPP2R3L

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Subunit structure	PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with N-terminal region of CDC6. Ref.3
Subcellular location	Nucleus Ref.3.
Miscellaneous	The gene encoding for this protein is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes.
Sequence similarities	Contains 1 EF-hand domain.
Sequence caution	The sequence AAD38515.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Calcium Metal-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G1/S transition of mitotic cell cycle Traceable author statement. Source: Reactome cell cycle arrest Traceable author statement Ref.3. Source: ProtInc protein dephosphorylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	nucleoplasm Traceable author statement. Source: Reactome protein phosphatase type 2A complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro protein phosphatase type 2A regulator activity Traceable author statement Ref.3. Source: ProtInc protein serine/threonine phosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y5P8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y5P8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-399: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta		PRO_0000071444
Regions
	Domain	388 – 423	36	EF-hand
	Calcium binding	401 – 412	12	Potential
Natural variations
	Alternative sequence	1 – 399	399	Missing in isoform 2.		VSP_037310
	Natural variant	163	1	D → E. Corresponds to variant rs3813594 [ dbSNP | Ensembl ].		VAR_035109
	Natural variant	519	1	A → V. Ref.2 Corresponds to variant rs1133520 [ dbSNP | Ensembl ].		VAR_055356

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 11, 2007. Version 2. Checksum: 9FDE046345C1F85F	FASTA	575	65,061
	10 20 30 40 50 60 MPPGKVLQPV LKMKVDELFL YWLSEASTQR MLQDCLRRIK APGRDQPTPG DGEQPGAWPT 70 80 90 100 110 120 APLAAPRPSG LEPPGTPGPG PALPLGAASS PRNAPHVRGT RRSAGTRVVQ TRKEEPLPPA 130 140 150 160 170 180 TSQSIPTFYF PRGRPQDSVN VDAVISKIES TFARFPHERA TMDDMGLVAK ACGCPLYWKG 190 200 210 220 230 240 PLFYGAGGER TGSVSVHKFV AMWRKILQNC HDDAAKFVHL LMSPGCNYLV QEDFVPFLQD 250 260 270 280 290 300 VVNTHPGLSF LKEASEFHSR YITTVIQRIF YAVNRSWSGR ITCAELRRSS FLQNVALLEE 310 320 330 340 350 360 EADINQLTEF FSYEHFYVIY CKFWELDTDH DLLIDADDLA RHNDHALSTK MIDRIFSGAV 370 380 390 400 410 420 TRGRKVQKEG KISYADFVWF LISEEDKKTP TSIEYWFRCM DLDGDGALSM FELEYFYEEQ 430 440 450 460 470 480 CRRLDSMAIE ALPFQDCLCQ MLDLVKPRTE GKITLQDLKR CKLANVFFDT FFNIEKYLDH 490 500 510 520 530 540 EQKEQISLLR DGDSGGPELS DWEKYAAEEY DILVAEETAG EPWEDGFEAE LSPVEQKLSA 550 560 570 LRSPLAQRPF FEAPSPLGAV DLYEYACGDE DLEPL
	Isoform 2 [UniParc]. Checksum: 6B819D28EBF3AE5F	FASTA	176	20,111
	10 20 30 40 50 60 MDLDGDGALS MFELEYFYEE QCRRLDSMAI EALPFQDCLC QMLDLVKPRT EGKITLQDLK 70 80 90 100 110 120 RCKLANVFFD TFFNIEKYLD HEQKEQISLL RDGDSGGPEL SDWEKYAAEE YDILVAEETA 130 140 150 160 170 GEPWEDGFEA ELSPVEQKLS ALRSPLAQRP FFEAPSPLGA VDLYEYACGD EDLEPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT VAL-519. Tissue: Brain.
[3]	"PR48, a novel regulatory subunit of protein phosphatase 2A, interacts with cdc6 and modulates DNA replication in human cells." Yan Z., Fedorov S.A., Mumby M.C., Williams R.S. Mol. Cell. Biol. 20:1021-1029(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 125-475, SUBCELLULAR LOCATION, INTERACTION WITH CDC6. Tissue: Placenta.
[4]	"Identification and characterization of B''-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues." Stevens I., Janssens V., Martens E., Dilworth S., Goris J., Van Hoof C. Eur. J. Biochem. 270:376-387(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF MISSING N-TERMINAL SEQUENCE.
[5]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL954664 Genomic DNA. No translation available. BX000476 Genomic DNA. No translation available. BC009032 mRNA. Translation: AAH09032.1. BC063429 mRNA. Translation: AAH63429.1. AF135016 mRNA. Translation: AAD38515.1. Different initiation. BK000521 mRNA. Translation: DAA00385.1.
IPI	IPI00399006. IPI00845329.
RefSeq	NP_037371.2. NM_013239.4.
UniGene	Hs.124942.
3D structure databases
ProteinModelPortal	Q9Y5P8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y5P8. 3 interactions.
STRING	9606.ENSP00000375080.
PTM databases
PhosphoSite	Q9Y5P8.
Polymorphism databases
DMDM	158523346.
Proteomic databases
PaxDb	Q9Y5P8.
PRIDE	Q9Y5P8.
Protocols and materials databases
DNASU	28227.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000390665; ENSP00000375080; ENSG00000167393.
GeneID	28227.
KEGG	hsa:28227.
UCSC	uc004cpf.3. human. uc004cpg.3. human.
Organism-specific databases
CTD	28227.
GeneCards	GC0XM000264.
H-InvDB	HIX0038850. HIX0176522. HIX0177602.
HGNC	HGNC:13417. PPP2R3B.
MIM	300339. gene.
neXtProt	NX_Q9Y5P8.
PharmGKB	PA134878872.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG271066.
HOGENOM	HOG000265086.
HOVERGEN	HBG000010.
InParanoid	Q9Y5P8.
KO	K11583.
OMA	ASATQIQ.
OrthoDB	EOG4TQM8T.
PhylomeDB	Q9Y5P8.
Enzyme and pathway databases
Reactome	REACT_115566. Cell Cycle.
Gene expression databases
ArrayExpress	Q9Y5P8.
Bgee	Q9Y5P8.
CleanEx	HS_PPP2R3B.
Genevestigator	Q9Y5P8.
GermOnline	ENSG00000167393. Homo sapiens.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. [Graphical view]
Pfam	PF13499. EF_hand_5. 1 hit. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	28227.
NextBio	50538.
SOURCE	Search...

Entry information

Entry name

P2R3B_HUMAN

Accession

Primary (citable) accession number: Q9Y5P8
Secondary accession number(s): Q6P4G9, Q7RTT1, Q96H01

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	September 11, 2007
Last modified:	May 1, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.