Q9Y5K3 (PCY1B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Choline-phosphate cytidylyltransferase B
EC=2.7.7.15

Alternative name(s):
CCT-beta
CTP:phosphocholine cytidylyltransferase B
Short name=CCT B
Short name=CT B
Phosphorylcholine transferase B

Gene names

Name:	PCYT1B
Synonyms:	CCTB

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Controls phosphatidylcholine synthesis.
Catalytic activity	CTP + choline phosphate = diphosphate + CDP-choline.
Pathway	Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.
Subcellular location	Endoplasmic reticulum.
Tissue specificity	Highly expressed in testis, placenta, brain, ovary and fetus.
Post-translational modification	Extensively phosphorylated. The beta-1 isoform seems to be much less phosphorylated. Ref.7 Ref.8
Sequence similarities	Belongs to the cytidylyltransferase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Endoplasmic reticulum
Coding sequence diversity	Alternative splicing
Molecular function	Nucleotidyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	choline-phosphate cytidylyltransferase activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Named isoforms=2.

Isoform 2 (identifier: Q9Y5K3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q9Y5K3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     321-369: VSSPTRSRSPSRSPSPTFSWLPLKTSPPSSPKAASASISSMSEGDEDEK → LKSWARCRDF

Isoform 3 (identifier: Q9Y5K3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-16: Missing.
     17-39: SLSNEPPSETMEEIEHTCPQPRL → MVGNQECIMEEDNRAPQLWRK

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Choline-phosphate cytidylyltransferase B		PRO_0000208456
Regions
	Region	74 – 235	162	Catalytic Potential
Amino acid modifications
	Modified residue	233	1	Phosphoserine Ref.8
	Modified residue	315	1	Phosphoserine Ref.7 Ref.8
	Modified residue	319	1	Phosphoserine By similarity
	Modified residue	325	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	1 – 16	16	Missing in isoform 3.		VSP_020045
	Alternative sequence	17 – 39	23	SLSNE…PQPRL → MVGNQECIMEEDNRAPQLWR K in isoform 3.		VSP_020046
	Alternative sequence	321 – 369	49	VSSPT…DEDEK → LKSWARCRDF in isoform 1.		VSP_001226

Sequences

	Sequence		Length	Mass (Da)
	Isoform 2 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 87A00617DF690DD1	FASTA	369	41,940
	10 20 30 40 50 60 MPVVTTDAES ETGIPKSLSN EPPSETMEEI EHTCPQPRLT LTAPAPFADE TNCQCQAPHE 70 80 90 100 110 120 KLTIAQARLG TPADRPVRVY ADGIFDLFHS GHARALMQAK TLFPNSYLLV GVCSDDLTHK 130 140 150 160 170 180 FKGFTVMNEA ERYEALRHCR YVDEVIRDAP WTLTPEFLEK HKIDFVAHDD IPYSSAGSDD 190 200 210 220 230 240 VYKHIKEAGM FVPTQRTEGI STSDIITRIV RDYDVYARRN LQRGYTAKEL NVSFINEKRY 250 260 270 280 290 300 RFQNQVDKMK EKVKNVEERS KEFVNRVEEK SHDLIQKWEE KSREFIGNFL ELFGPDGAWK 310 320 330 340 350 360 QMFQERSSRM LQALSPKQSP VSSPTRSRSP SRSPSPTFSW LPLKTSPPSS PKAASASISS MSEGDEDEK
	Isoform 1 [UniParc]. Checksum: C1A16BC1B61906B2	FASTA	330	38,086
	10 20 30 40 50 60 MPVVTTDAES ETGIPKSLSN EPPSETMEEI EHTCPQPRLT LTAPAPFADE TNCQCQAPHE 70 80 90 100 110 120 KLTIAQARLG TPADRPVRVY ADGIFDLFHS GHARALMQAK TLFPNSYLLV GVCSDDLTHK 130 140 150 160 170 180 FKGFTVMNEA ERYEALRHCR YVDEVIRDAP WTLTPEFLEK HKIDFVAHDD IPYSSAGSDD 190 200 210 220 230 240 VYKHIKEAGM FVPTQRTEGI STSDIITRIV RDYDVYARRN LQRGYTAKEL NVSFINEKRY 250 260 270 280 290 300 RFQNQVDKMK EKVKNVEERS KEFVNRVEEK SHDLIQKWEE KSREFIGNFL ELFGPDGAWK 310 320 330 QMFQERSSRM LQALSPKQSP LKSWARCRDF
	Isoform 3 [UniParc]. Checksum: 0E2BBEDD2569EAA8	FASTA	351	40,206
	10 20 30 40 50 60 MVGNQECIME EDNRAPQLWR KTLTAPAPFA DETNCQCQAP HEKLTIAQAR LGTPADRPVR 70 80 90 100 110 120 VYADGIFDLF HSGHARALMQ AKTLFPNSYL LVGVCSDDLT HKFKGFTVMN EAERYEALRH 130 140 150 160 170 180 CRYVDEVIRD APWTLTPEFL EKHKIDFVAH DDIPYSSAGS DDVYKHIKEA GMFVPTQRTE 190 200 210 220 230 240 GISTSDIITR IVRDYDVYAR RNLQRGYTAK ELNVSFINEK RYRFQNQVDK MKEKVKNVEE 250 260 270 280 290 300 RSKEFVNRVE EKSHDLIQKW EEKSREFIGN FLELFGPDGA WKQMFQERSS RMLQALSPKQ 310 320 330 340 350 SPVSSPTRSR SPSRSPSPTF SWLPLKTSPP SSPKAASASI SSMSEGDEDE K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a second human CTP:phosphocholine cytidylyltransferase." Lykidis A., Murti K.G., Jackowski S. J. Biol. Chem. 273:14022-14029(1998) [PubMed: 9593753] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Distribution of CTP:phosphocholine cytidylyltransferase (CCT) isoforms. Identification of a new CCTbeta splice variant." Lykidis A., Baburina I., Jackowski S. J. Biol. Chem. 274:26992-27001(1999) [PubMed: 10480912] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), CHARACTERIZATION.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Hippocampus.
[4]	SeattleSNPs variation discovery resource Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain.
[7]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-233 AND SER-315, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Web resources

SeattleSNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF052510 mRNA. Translation: AAC39754.1. AF148464 mRNA. Translation: AAD35088.1. AK315323 mRNA. Translation: BAG37725.1. EU181262 Genomic DNA. Translation: ABW03924.1. CH471074 Genomic DNA. Translation: EAW99021.1. BC045634 mRNA. Translation: AAH45634.2.
IPI	IPI00001562. IPI00219338. IPI00640025.
RefSeq	NP_001156736.1. NM_001163264.1. NP_001156737.1. NM_001163265.1. NP_004836.2. NM_004845.4.
UniGene	Hs.660708.
3D structure databases
ProteinModelPortal	Q9Y5K3.
SMR	Q9Y5K3. Positions 41-216, 236-268.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9Y5K3.
PTM databases
PhosphoSite	Q9Y5K3.
Polymorphism databases
DMDM	12643330.
Proteomic databases
PRIDE	Q9Y5K3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000379144; ENSP00000368439; ENSG00000102230.
GeneID	9468.
KEGG	hsa:9468.
UCSC	uc004dbi.1. human. uc004dbk.2. human.
Organism-specific databases
CTD	9468.
GeneCards	GC0XM024486.
H-InvDB	HIX0016708.
HGNC	HGNC:8755. PCYT1B.
HPA	HPA006367.
MIM	604926. gene.
neXtProt	NX_Q9Y5K3.
PharmGKB	PA33100.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG13522.
GeneTree	ENSGT00390000000269.
HOGENOM	HBG587468.
HOVERGEN	HBG053531.
InParanoid	Q9Y5K3.
OMA	CRAPHEK.
OrthoDB	EOG45X7WJ.
PhylomeDB	Q9Y5K3.
Gene expression databases
ArrayExpress	Q9Y5K3.
Bgee	Q9Y5K3.
CleanEx	HS_PCYT1B.
Genevestigator	Q9Y5K3.
GermOnline	ENSG00000102230. Homo sapiens.
Family and domain databases
InterPro	IPR004821. Cyt_trans-rel. IPR004820. Cytidylyltransf. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K00968.
Pfam	PF01467. CTP_transf_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00125. Cyt_tran_rel. 1 hit.
ProtoNet	Search...
Other
DrugBank	DB00122. Choline.
NextBio	35482.
SOURCE	Search...

Entry information

Entry name

PCY1B_HUMAN

Accession

Primary (citable) accession number: Q9Y5K3
Secondary accession number(s): A8IX00 , B2RCX8, O60621, Q86XC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	November 1, 1999
Last modified:	January 25, 2012
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.