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Q9Y5J9

- TIM8B_HUMAN

UniProt

Q9Y5J9 - TIM8B_HUMAN

Protein

Mitochondrial import inner membrane translocase subunit Tim8 B

Gene

TIMM8B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Probable mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space By similarity.By similarity

    GO - Molecular functioni

    1. zinc ion binding Source: ProtInc

    GO - Biological processi

    1. cellular protein metabolic process Source: Reactome
    2. chaperone-mediated protein transport Source: BHF-UCL
    3. protein targeting to mitochondrion Source: Reactome
    4. sensory perception of sound Source: ProtInc

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Protein transport, Translocation, Transport

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_118595. Mitochondrial protein import.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial import inner membrane translocase subunit Tim8 B
    Alternative name(s):
    DDP-like protein
    Deafness dystonia protein 2
    Gene namesi
    Name:TIMM8B
    Synonyms:DDP2, DDPL, TIM8B
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:11818. TIMM8B.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProt
    2. mitochondrial inner membrane Source: UniProtKB-SubCell
    3. mitochondrial intermembrane space protein transporter complex Source: BHF-UCL

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA36524.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed3 Publications
    Chaini2 – 8382Mitochondrial import inner membrane translocase subunit Tim8 BPRO_0000193587Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine3 Publications
    Disulfide bondi36 ↔ 59By similarity
    Disulfide bondi40 ↔ 55By similarity

    Keywords - PTMi

    Acetylation, Disulfide bond

    Proteomic databases

    MaxQBiQ9Y5J9.
    PaxDbiQ9Y5J9.
    PeptideAtlasiQ9Y5J9.
    PRIDEiQ9Y5J9.

    PTM databases

    PhosphoSiteiQ9Y5J9.

    Expressioni

    Tissue specificityi

    Ubiquitous, with highest expression in heart, kidney, liver and skeletal muscle.1 Publication

    Gene expression databases

    ArrayExpressiQ9Y5J9.
    BgeeiQ9Y5J9.
    CleanExiHS_TIMM8B.
    GenevestigatoriQ9Y5J9.

    Organism-specific databases

    HPAiHPA041173.

    Interactioni

    Subunit structurei

    Heterohexamer; possibly composed of 3 copies of TIMM8B and 3 copies of TIMM13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIMM22 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi117725. 6 interactions.
    IntActiQ9Y5J9. 3 interactions.
    STRINGi9606.ENSP00000280354.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Y5J9.
    SMRiQ9Y5J9. Positions 20-72.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi36 – 5924Twin CX3C motifAdd
    BLAST

    Domaini

    The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIMM8B from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane By similarity.By similarity

    Sequence similaritiesi

    Belongs to the small Tim family.Curated

    Phylogenomic databases

    eggNOGiNOG238740.
    HOGENOMiHOG000115758.
    HOVERGENiHBG060492.
    InParanoidiQ9Y5J9.
    KOiK17780.
    OrthoDBiEOG79W97P.
    PhylomeDBiQ9Y5J9.

    Family and domain databases

    Gene3Di1.10.287.810. 1 hit.
    InterProiIPR004217. Tim10/DDP_fam_Znf.
    [Graphical view]
    PfamiPF02953. zf-Tim10_DDP. 1 hit.
    [Graphical view]
    SUPFAMiSSF144122. SSF144122. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9Y5J9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAELGEADEA ELQRLVAAEQ QKAQFTAQVH HFMELCWDKC VEKPGNRLDS   50
    RTENCLSSCV DRFIDTTLAI TSRFAQIVQK GGQ 83
    Length:83
    Mass (Da):9,344
    Last modified:November 1, 1999 - v1
    Checksum:i9DC47BB475DB8692
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF152350 mRNA. Translation: AAF15100.1.
    AF150087 mRNA. Translation: AAD39994.1.
    AK312169 mRNA. Translation: BAG35103.1.
    EF445036 Genomic DNA. Translation: ACA06083.1.
    BC000711 mRNA. Translation: AAH00711.1.
    BC105986 mRNA. Translation: AAI05987.1.
    BC106067 mRNA. Translation: AAI06068.1.
    AF165967 mRNA. Translation: AAD51801.1.
    RefSeqiNP_036591.2. NM_012459.2.
    UniGeneiHs.279915.

    Genome annotation databases

    EnsembliENST00000504148; ENSP00000422122; ENSG00000150779.
    GeneIDi26521.
    KEGGihsa:26521.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF152350 mRNA. Translation: AAF15100.1 .
    AF150087 mRNA. Translation: AAD39994.1 .
    AK312169 mRNA. Translation: BAG35103.1 .
    EF445036 Genomic DNA. Translation: ACA06083.1 .
    BC000711 mRNA. Translation: AAH00711.1 .
    BC105986 mRNA. Translation: AAI05987.1 .
    BC106067 mRNA. Translation: AAI06068.1 .
    AF165967 mRNA. Translation: AAD51801.1 .
    RefSeqi NP_036591.2. NM_012459.2.
    UniGenei Hs.279915.

    3D structure databases

    ProteinModelPortali Q9Y5J9.
    SMRi Q9Y5J9. Positions 20-72.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 117725. 6 interactions.
    IntActi Q9Y5J9. 3 interactions.
    STRINGi 9606.ENSP00000280354.

    PTM databases

    PhosphoSitei Q9Y5J9.

    Proteomic databases

    MaxQBi Q9Y5J9.
    PaxDbi Q9Y5J9.
    PeptideAtlasi Q9Y5J9.
    PRIDEi Q9Y5J9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000504148 ; ENSP00000422122 ; ENSG00000150779 .
    GeneIDi 26521.
    KEGGi hsa:26521.

    Organism-specific databases

    CTDi 26521.
    GeneCardsi GC11M111989.
    H-InvDB HIX0201613.
    HGNCi HGNC:11818. TIMM8B.
    HPAi HPA041173.
    MIMi 606659. gene.
    neXtProti NX_Q9Y5J9.
    PharmGKBi PA36524.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG238740.
    HOGENOMi HOG000115758.
    HOVERGENi HBG060492.
    InParanoidi Q9Y5J9.
    KOi K17780.
    OrthoDBi EOG79W97P.
    PhylomeDBi Q9Y5J9.

    Enzyme and pathway databases

    Reactomei REACT_118595. Mitochondrial protein import.

    Miscellaneous databases

    GenomeRNAii 26521.
    NextBioi 48842.
    PROi Q9Y5J9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Y5J9.
    Bgeei Q9Y5J9.
    CleanExi HS_TIMM8B.
    Genevestigatori Q9Y5J9.

    Family and domain databases

    Gene3Di 1.10.287.810. 1 hit.
    InterProi IPR004217. Tim10/DDP_fam_Znf.
    [Graphical view ]
    Pfami PF02953. zf-Tim10_DDP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF144122. SSF144122. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The human family of deafness/dystonia peptide (DDP) related mitochondrial import proteins."
      Jin H., Kendall E., Freeman T.C., Roberts R.G., Vetrie D.L.P.
      Genomics 61:259-267(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The mitochondrial TIM22 preprotein translocase is highly conserved throughout the eukaryotic kingdom."
      Bauer M.F., Rothbauer U., Muehlenbein N., Smith R.J.H., Gerbitz K.-D., Neupert W., Brunner M., Hofmann S.
      FEBS Lett. 464:41-47(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    4. NHLBI resequencing and genotyping service (RS&G)
      Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung, Mammary gland and Testis.
    6. "Molecular cloning of DDP-like gene."
      Xia J.-H., He Y.-G., Yu K.-P., Zheng Z.-H., Tan S.
      Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-83.
      Tissue: Heart.
    7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTIM8B_HUMAN
    AccessioniPrimary (citable) accession number: Q9Y5J9
    Secondary accession number(s): B0YJA5, Q3KQS9, Q9UN04
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3