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Q9Y5B6 (GCFC1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GC-rich sequence DNA-binding factor 1
Gene names
Name:GCFC1
Synonyms:C21orf66, GCFC
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length917 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Possible transcription factor.

Subcellular location

Nucleus Potential.

Tissue specificity

Ubiquitous.

Sequence similarities

Belongs to the GCF family.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   LigandDNA-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processtranscription, DNA-dependent

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytosol

Inferred from direct assay. Source: UniProtKB

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionDNA binding

Non-traceable author statement. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: Q9Y5B6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform B (identifier: Q9Y5B6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     779-917: LLGNFLQWYG...KEFKSLIEGK → VIKPPFQRGS...WTDRPCVVFS
Isoform C (identifier: Q9Y5B6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     462-469: VPLINELE → SVQFRKLL
     470-917: Missing.
Isoform D (identifier: Q9Y5B6-4)

The sequence of this isoform differs from the canonical sequence as follows:
     503-511: NKALMAPNL → SQSILKTKL
     512-917: Missing.
Note: Major isoform.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 917917GC-rich sequence DNA-binding factor 1
PRO_0000087439

Amino acid modifications

Modified residue161Phosphoserine Ref.7
Modified residue621Phosphoserine Ref.6
Modified residue1541Phosphoserine Ref.9
Modified residue1551Phosphoserine Ref.9
Modified residue1581Phosphoserine Ref.9
Modified residue1911Phosphoserine Ref.7
Modified residue2621Phosphoserine Ref.9 Ref.10 Ref.11
Modified residue2951Phosphoserine Ref.12
Modified residue3781Phosphothreonine Ref.7
Modified residue3801Phosphoserine Ref.7
Modified residue5571Phosphoserine Ref.6 Ref.7 Ref.8 Ref.9 Ref.11 Ref.12
Modified residue5581Phosphoserine Ref.6 Ref.7 Ref.8 Ref.9 Ref.11 Ref.12

Natural variations

Alternative sequence462 – 4698VPLINELE → SVQFRKLL in isoform C.
VSP_004263
Alternative sequence470 – 917448Missing in isoform C.
VSP_004264
Alternative sequence503 – 5119NKALMAPNL → SQSILKTKL in isoform D.
VSP_004265
Alternative sequence512 – 917406Missing in isoform D.
VSP_004266
Alternative sequence779 – 917139LLGNF…LIEGK → VIKPPFQRGSCPIPRRKECC SERPRRIWTDRPCVVFS in isoform B.
VSP_004267

Experimental info

Sequence conflict692 – 71120Missing in AAD34617. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified December 19, 2001. Version 2.
Checksum: 855960A1D50A7789

FASTA917104,804
        10         20         30         40         50         60 
MFRKARRVNV RKRNDSEEEE RERDEEQEPP PLLPPPGTGE EAGPGGGDRA PGGESLLGPG 

        70         80         90        100        110        120 
PSPPSALTPG LGAEAGGGFP GGAEPGNGLK PRKRPRENKE VPRASLLSFQ DEEEENEEVF 

       130        140        150        160        170        180 
KVKKSSYSKK IVKLLKKEYK EDLEKSKIKT ELNSSAESEQ PLDKTGHVKD TNQEDGVIIS 

       190        200        210        220        230        240 
EHGEDEMDME SEKEEEKPKT GGAFSNALSS LNVLRPGEIP DAAFIHAARK KRQMARELGD 

       250        260        270        280        290        300 
FTPHDNEPGK GRLVREDEND ASDDEDDDEK RRIVFSVKEK SQRQKIAEEI GIEGSDDDAL 

       310        320        330        340        350        360 
VTGEQDEELS RWEQEQIRKG INIPQVQASQ PAEVNMYYQN TYQTMPYGSS YGIPYSYTAY 

       370        380        390        400        410        420 
GSSDAKSQKT DNTVPFKTPS NEMTPVTIDL VKKQLKDRLD SMKELHKTNR QQHEKHLQSR 

       430        440        450        460        470        480 
VDSTRAIERL EGSSGGIGER YKFLQEMRGY VQDLLECFSE KVPLINELES AIHQLYKQRA 

       490        500        510        520        530        540 
SRLVQRRQDD IKDESSEFSS HSNKALMAPN LDSFGRDRAL YQEHAKRRIA EREARRTRRR 

       550        560        570        580        590        600 
QAREQTGKMA DHLEGLSSDD EETSTDITNF NLEKDRISKE SGKVFEDVLE SFYSIDCIKS 

       610        620        630        640        650        660 
QFEAWRSKYY TSYKDAYIGL CLPKLFNPLI RLQLLTWTPL EAKCRDFENM LWFESLLFYG 

       670        680        690        700        710        720 
CEEREQEKDD VDVALLPTIV EKVILPKLTV IAENMWDPFS TTQTSRMVGI TLKLINGYPS 

       730        740        750        760        770        780 
VVNAENKNTQ VYLKALLLRM RRTLDDDVFM PLYPKNVLEN KNSGPYLFFQ RQFWSSVKLL 

       790        800        810        820        830        840 
GNFLQWYGIF SNKTLQELSI DGLLNRYILM AFQNSEYGDD SIKKAQNVIN CFPKQWFMNL 

       850        860        870        880        890        900 
KGERTISQLE NFCRYLVHLA DTIYRNSIGC SDVEKRNARE NIKQIVKLLA SVRALDHAMS 

       910 
VASDHNVKEF KSLIEGK

« Hide

Isoform B [UniParc].

Checksum: A2843B97BBEA753E
Show »

FASTA81593,170
Isoform C [UniParc].

Checksum: 96B04F480DFFAEDD
Show »

FASTA46952,611
Isoform D [UniParc].

Checksum: 2D90273749BD50FE
Show »

FASTA51157,431

References

« Hide 'large scale' references
[1]"From PREDs and open reading frames to cDNA isolation: revisiting the human chromosome 21 transcription map."
Reymond A., Friedli M., Neergaard Henrichsen C., Chapot F., Deutsch S., Ucla C., Rossier C., Lyle R., Guipponi M., Antonarakis S.E.
Genomics 78:46-54(2001) [PubMed: 11707072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B; C AND D).
[2]"Isolation and initial characterization of a putative human chromosome 21 transcription factor."
Chapot-Skovsgaard F.M., Guipponi M., Lyle R., Antonarakis S.E.
Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND D).
Tissue: Brain, Heart, Kidney, Lung, Muscle, Placenta and Testis.
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Criteria for gene identification and features of genome organization: analysis of 6.5 Mb of DNA sequence from human chromosome 21."
Slavov D., Hattori M., Sakaki Y., Rosenthal A., Shimizu N., Minoshima S., Kudoh J., Yaspo M.-L., Ramser J., Reinhardt R., Reimer C., Clancy K., Rynditch A., Gardiner K.
Gene 247:215-232(2000) [PubMed: 10773462] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 132-917 (ISOFORM A).
[5]"Cloning of candidate of GC-rich sequence DNA-binding factor."
Teramoto T., Thorgeirsson S.S.
Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 489-815 (ISOFORM B).
[6]"Large-scale characterization of HeLa cell nuclear phosphoproteins."
Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62; SER-557 AND SER-558, MASS SPECTROMETRY.
Tissue: Cervix carcinoma.
[7]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed: 17081983] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16; SER-191; THR-378; SER-380; SER-557 AND SER-558, MASS SPECTROMETRY.
Tissue: Cervix carcinoma.
[8]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-557 AND SER-558, MASS SPECTROMETRY.
Tissue: Cervix carcinoma.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-154; SER-155; SER-158; SER-262; SER-557 AND SER-558, MASS SPECTROMETRY.
Tissue: Cervix carcinoma.
[10]"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."
Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J.
Proteomics 8:1346-1361(2008) [PubMed: 18318008] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-262, MASS SPECTROMETRY.
Tissue: Liver.
[11]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-262; SER-557 AND SER-558, MASS SPECTROMETRY.
Tissue: Embryonic kidney.
[12]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-295; SER-557 AND SER-558, MASS SPECTROMETRY.
Tissue: Leukemic T-cell.
[13]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY033903 mRNA. Translation: AAK68721.1.
AY033904 mRNA. Translation: AAK68722.1.
AY033905 mRNA. Translation: AAK68723.1.
AY033906 mRNA. Translation: AAK68724.1.
AJ279080 mRNA. Translation: CAC40813.1.
AJ279081 mRNA. Translation: CAC40814.1.
CH471079 Genomic DNA. Translation: EAX09859.1.
CH471079 Genomic DNA. Translation: EAX09861.1.
AF231920 mRNA. Translation: AAF72944.1.
AF153208 mRNA. Translation: AAD34617.1. Different termination.
IPIIPI00001364.
IPI00069267.
IPI00219169.
IPI00797180.
RefSeqNP_037461.2. NM_013329.3.
NP_057715.2. NM_016631.3.
UniGeneHs.644004.

3D structure databases

ProteinModelPortalQ9Y5B6.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9Y5B6. 2 interactions.
STRINGQ9Y5B6.

PTM databases

PhosphoSiteQ9Y5B6.

Polymorphism databases

DMDM20141448.

Proteomic databases

PRIDEQ9Y5B6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000331923; ENSP00000328992; ENSG00000159086.
GeneID94104.
KEGGhsa:94104.
UCSCuc002yqn.1. human.
uc002yqp.1. human.
uc002yqq.1. human.

Organism-specific databases

CTD94104.
GeneCardsGC21M034106.
H-InvDBHIX0016067.
HGNCHGNC:13579. GCFC1.
HPAHPA003757.
neXtProtNX_Q9Y5B6.
PharmGKBPA25861.
GenAtlasSearch...

Phylogenomic databases

GeneTreeENSGT00390000000455.
HOGENOMHBG443575.
HOVERGENHBG005817.
InParanoidQ9Y5B6.
OMAFTPHDNE.
OrthoDBEOG4TF0JN.
PhylomeDBQ9Y5B6.

Gene expression databases

ArrayExpressQ9Y5B6.
BgeeQ9Y5B6.
CleanExHS_C21orf66.
GenevestigatorQ9Y5B6.
GermOnlineENSG00000159086. Homo sapiens.

Family and domain databases

InterProIPR012890. GCFC.
IPR022783. GCFC_dom.
[Graphical view]
KOK13211.
PANTHERPTHR12214. GCFC. 1 hit.
PfamPF07842. GCFC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio78383.

Entry information

Entry nameGCFC1_HUMAN
AccessionPrimary (citable) accession number: Q9Y5B6
Secondary accession number(s): D3DSE7, Q96DU8, Q9NYQ0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: December 19, 2001
Last modified: January 25, 2012
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 21

Human chromosome 21: entries, gene names and cross-references to MIM

SIMILARITY comments

Index of protein domains and families

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