Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

RNA polymerase II subunit A C-terminal domain phosphatase

Gene

CTDP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit. This promotes the activity of RNA polymerase II. Plays a role in the exit from mitosis by dephosphorylating crucial mitotic substrates (USP44, CDC20 and WEE1) that are required for M-phase-promoting factor (MPF)/CDK1 inactivation.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

GO - Molecular functioni

  • CTD phosphatase activity Source: UniProtKB
  • phosphoprotein phosphatase activity Source: Reactome
  • Tat protein binding Source: CAFA
  • TFIIF-class transcription factor binding Source: CAFA

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-75955 RNA Polymerase II Transcription Elongation
SIGNORiQ9Y5B0

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase II subunit A C-terminal domain phosphatase (EC:3.1.3.16)
Alternative name(s):
TFIIF-associating CTD phosphatase
Gene namesi
Name:CTDP1
Synonyms:FCP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

EuPathDBiHostDB:ENSG00000060069.16
HGNCiHGNC:2498 CTDP1
MIMi604927 gene
neXtProtiNX_Q9Y5B0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Involvement in diseasei

Congenital cataracts, facial dysmorphism, and neuropathy (CCFDN)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn autosomal recessive developmental disorder characterized by a complex clinical phenotype with seemingly unrelated features involving multiple organs and systems. Developmental abnormalities include congenital cataracts and microcorneae, hypomyelination of the peripheral nervous system, impaired physical growth, delayed early motor and intellectual development, facial dysmorphism and hypogonadism. Central nervous system involvement, with cerebral and spinal cord atrophy, may be the result of disrupted development with superimposed degenerative changes. Affected individuals are prone to severe rhabdomyolysis after viral infections and to serious complications related to general anesthesia (such as pulmonary edema and epileptic seizures).
See also OMIM:604168

Keywords - Diseasei

Cataract

Organism-specific databases

DisGeNETi9150
GeneReviewsiCTDP1
MalaCardsiCTDP1
MIMi604168 phenotype
OpenTargetsiENSG00000060069
Orphaneti48431 Congenital cataracts - facial dysmorphism - neuropathy
PharmGKBiPA27001

Polymorphism and mutation databases

BioMutaiCTDP1
DMDMi327478586

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002125641 – 961RNA polymerase II subunit A C-terminal domain phosphataseAdd BLAST961

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei395PhosphoserineCombined sources1
Modified residuei674PhosphoserineCombined sources1
Modified residuei740PhosphoserineCombined sources1
Modified residuei780N6-acetyllysineCombined sources1
Modified residuei839PhosphoserineCombined sources1
Modified residuei869PhosphoserineCombined sources1
Modified residuei872PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated. In the presence of TFIIF, the phosphorylated form has an increased CTD phosphatase activity. The phosphorylation is required for the physical interaction with GTF2F1.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9Y5B0
PaxDbiQ9Y5B0
PeptideAtlasiQ9Y5B0
PRIDEiQ9Y5B0

PTM databases

DEPODiQ9Y5B0
iPTMnetiQ9Y5B0
PhosphoSitePlusiQ9Y5B0

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000060069
CleanExiHS_CTDP1
ExpressionAtlasiQ9Y5B0 baseline and differential
GenevisibleiQ9Y5B0 HS

Organism-specific databases

HPAiCAB032641
HPA040394

Interactioni

Subunit structurei

Homodimer. Interacts with GTF2F1. Interacts with WDR77, SNRPB and SNRNP70.3 Publications

GO - Molecular functioni

  • Tat protein binding Source: CAFA
  • TFIIF-class transcription factor binding Source: CAFA

Protein-protein interaction databases

BioGridi114597, 58 interactors
CORUMiQ9Y5B0
DIPiDIP-41788N
IntActiQ9Y5B0, 14 interactors
MINTiQ9Y5B0
STRINGi9606.ENSP00000299543

Structurei

Secondary structure

1961
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi586 – 598Combined sources13
Helixi945 – 956Combined sources12
Turni957 – 959Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J2XX-ray2.00B944-961[»]
1ONVNMR-B879-961[»]
2K7LNMR-B582-600[»]
DisProtiDP00177
ProteinModelPortaliQ9Y5B0
SMRiQ9Y5B0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y5B0

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini178 – 344FCP1 homologyPROSITE-ProRule annotationAdd BLAST167
Domaini629 – 728BRCTPROSITE-ProRule annotationAdd BLAST100

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi455 – 478Ser-richAdd BLAST24
Compositional biasi577 – 582Poly-Glu6

Phylogenomic databases

eggNOGiKOG0323 Eukaryota
COG5190 LUCA
GeneTreeiENSGT00390000015641
HOGENOMiHOG000112039
HOVERGENiHBG051213
InParanoidiQ9Y5B0
KOiK15732
OMAiTREHYHA
OrthoDBiEOG091G05JF
PhylomeDBiQ9Y5B0
TreeFamiTF315104

Family and domain databases

CDDicd00027 BRCT, 1 hit
Gene3Di3.40.50.1000, 1 hit
3.40.50.10190, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR015388 FCP1_C
IPR004274 FCP1_dom
IPR011947 FCP1_euk
IPR036412 HAD-like_sf
IPR023214 HAD_sf
PfamiView protein in Pfam
PF09309 FCP1_C, 1 hit
PF03031 NIF, 1 hit
SMARTiView protein in SMART
SM00292 BRCT, 1 hit
SM00577 CPDc, 1 hit
SUPFAMiSSF52113 SSF52113, 1 hit
SSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR02250 FCP1_euk, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
PS50969 FCP1, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y5B0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEVPAAGRVP AEGAPTAAVA EVRCPGPAPL RLLEWRVAAG AAVRIGSVLA
60 70 80 90 100
VFEAAASAQS SGASQSRVAS GGCVRPARPE RRLRSERAGV VRELCAQPGQ
110 120 130 140 150
VVAPGAVLVR LEGCSHPVVM KGLCAECGQD LTQLQSKNGK QQVPLSTATV
160 170 180 190 200
SMVHSVPELM VSSEQAEQLG REDQQRLHRN RKLVLMVDLD QTLIHTTEQH
210 220 230 240 250
CQQMSNKGIF HFQLGRGEPM LHTRLRPHCK DFLEKIAKLY ELHVFTFGSR
260 270 280 290 300
LYAHTIAGFL DPEKKLFSHR ILSRDECIDP FSKTGNLRNL FPCGDSMVCI
310 320 330 340 350
IDDREDVWKF APNLITVKKY VYFQGTGDMN APPGSRESQT RKKVNHSRGT
360 370 380 390 400
EVSEPSPPVR DPEGVTQAPG VEPSNGLEKP ARELNGSEAA TPRDSPRPGK
410 420 430 440 450
PDERDIWPPA QAPTSSQELA GAPEPQGSCA QGGRVAPGQR PAQGATGTDL
460 470 480 490 500
DFDLSSDSES SSESEGTKSS SSASDGESEG KRGRQKPKAA PEGAGALAQG
510 520 530 540 550
SSLEPGRPAA PSLPGEAEPG AHAPDKEPEL GGQEEGERDG LCGLGNGCAD
560 570 580 590 600
RKEAETESQN SELSGVTAGE SLDQSMEEEE EEDTDEDDHL IYLEEILVRV
610 620 630 640 650
HTDYYAKYDR YLNKEIEEAP DIRKIVPELK SKVLADVAII FSGLHPTNFP
660 670 680 690 700
IEKTREHYHA TALGAKILTR LVLSPDAPDR ATHLIAARAG TEKVLQAQEC
710 720 730 740 750
GHLHVVNPDW LWSCLERWDK VEEQLFPLRD DHTKAQRENS PAAFPDREGV
760 770 780 790 800
PPTALFHPMP VLPKAQPGPE VRIYDSNTGK LIRTGARGPP APSSSLPIRQ
810 820 830 840 850
EPSSFRAVPP PQPQMFGEEL PDAQDGEQPG PSRRKRQPSM SETMPLYTLC
860 870 880 890 900
KEDLESMDKE VDDILGEGSD DSDSEKRRPE EQEEEPQPRK PGTRRERTLG
910 920 930 940 950
APASSERSAA GGRGPRGHKR KLNEEDAASE SSRESSNEDE GSSSEADEMA
960
KALEAELNDL M
Length:961
Mass (Da):104,399
Last modified:April 5, 2011 - v3
Checksum:i62B88FFD9CE4B157
GO
Isoform 4 (identifier: Q9Y5B0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     807-961: AVPPPQPQMF...ALEAELNDLM → WTTSLEKAAT...AGGPEATRGS

Show »
Length:867
Mass (Da):93,485
Checksum:i8B4B87B24644BA6E
GO

Sequence cautioni

The sequence AAC64549 differs from that shown. Cloning artifact.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61S → A in AAD42088 (PubMed:10385623).Curated1
Sequence conflicti61S → A in AAH63447 (PubMed:15489334).Curated1
Sequence conflicti157P → A in AAC64549 (PubMed:9765293).Curated1
Sequence conflicti281F → I in AAH52576 (PubMed:15489334).Curated1
Sequence conflicti305E → K in AAD42088 (PubMed:10385623).Curated1
Sequence conflicti390A → P in AAC64549 (PubMed:9765293).Curated1
Sequence conflicti478S → T in AAC64549 (PubMed:9765293).Curated1
Sequence conflicti486 – 487KP → NA in AAC64549 (PubMed:9765293).Curated2
Sequence conflicti504 – 505EP → DA in AAC64549 (PubMed:9765293).Curated2
Sequence conflicti513L → V in AAC64549 (PubMed:9765293).Curated1
Sequence conflicti656 – 657EH → DD in AAC64549 (PubMed:9765293).Curated2
Sequence conflicti896 – 900ERTLG → GADAR in AAD42088 (PubMed:10385623).Curated5

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_060440282S → F. Corresponds to variant dbSNP:rs4799078Ensembl.1
Natural variantiVAR_018264340T → M. Corresponds to variant dbSNP:rs2279103EnsemblClinVar.1
Natural variantiVAR_060441519P → H. Corresponds to variant dbSNP:rs557503Ensembl.1
Natural variantiVAR_032763755L → S. Corresponds to variant dbSNP:rs34967023Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009865807 – 961AVPPP…LNDLM → WTTSLEKAATTATARRGGLR SRRRSPSPGSQGPAGSGRSG HLRPARGARQGAGGPEATRG S in isoform 4. 2 PublicationsAdd BLAST155

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF154115 mRNA Translation: AAD42088.1
AC021594 Genomic DNA No translation available.
AC068473 Genomic DNA No translation available.
CH471117 Genomic DNA Translation: EAW66631.1
BC015010 mRNA Translation: AAH15010.1
BC052576 mRNA Translation: AAH52576.1
BC063447 mRNA Translation: AAH63447.1
AF081287 mRNA Translation: AAC64549.1 Sequence problems.
CCDSiCCDS12017.1 [Q9Y5B0-1]
CCDS12018.1 [Q9Y5B0-4]
RefSeqiNP_001189433.1, NM_001202504.1
NP_004706.3, NM_004715.4 [Q9Y5B0-1]
NP_430255.2, NM_048368.3 [Q9Y5B0-4]
UniGeneiHs.465490
Hs.734021

Genome annotation databases

EnsembliENST00000075430; ENSP00000075430; ENSG00000060069 [Q9Y5B0-4]
ENST00000613122; ENSP00000484525; ENSG00000060069 [Q9Y5B0-1]
GeneIDi9150
KEGGihsa:9150
UCSCiuc002lnh.3 human [Q9Y5B0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCTDP1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y5B0
Secondary accession number(s): A8MY97
, Q7Z644, Q96BZ1, Q9Y6F5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 5, 2011
Last modified: May 23, 2018
This is version 173 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health