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Protein

NEDD8 ultimate buster 1

Gene

NUB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2.1 Publication

GO - Biological processi

  • positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
  • protein ubiquitination Source: UniProtKB
  • response to interferon-gamma Source: UniProtKB
  • response to tumor necrosis factor Source: UniProtKB
  • ubiquitin-dependent protein catabolic process Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000013374-MONOMER.
ZFISH:ENSG00000103274-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8 ultimate buster 1
Alternative name(s):
Negative regulator of ubiquitin-like proteins 1
Renal carcinoma antigen NY-REN-18
Gene namesi
Name:NUB1
Synonyms:NYREN18
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Unplaced

Organism-specific databases

HGNCiHGNC:17623. NUB1.

Subcellular locationi

  • Nucleus 1 Publication

  • Note: Predominantly nuclear.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi448A → V: No effect on NEDD8-binding. 1 Publication1
Mutagenesisi453L → A: Partial inhibition of NEDD8-binding. 1 Publication1
Mutagenesisi464L → A: Partial inhibition of NEDD8-binding. 1 Publication1
Mutagenesisi468L → A: Partial inhibition of NEDD8-binding. 1 Publication1
Mutagenesisi587L → A: Suppression of NEDD8-binding; when associated with A-464; A-468 and A-591. Suppression of NEDD8-buster function; when associated with A-591. 1 Publication1
Mutagenesisi591L → A: Suppression of NEDD8-binding; when associated with A-464; A-468 and A-587. Suppression of NEDD8-buster function; when associated with A-587. 1 Publication1

Organism-specific databases

DisGeNETi51667.
PharmGKBiPA147357533.

Polymorphism and mutation databases

BioMutaiNUB1.
DMDMi37154888.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002109921 – 615NEDD8 ultimate buster 1Add BLAST615

Proteomic databases

PaxDbiQ9Y5A7.
PeptideAtlasiQ9Y5A7.
PRIDEiQ9Y5A7.

PTM databases

iPTMnetiQ9Y5A7.
PhosphoSitePlusiQ9Y5A7.

Expressioni

Tissue specificityi

Widely expressed with lowest expression in the pancreas for isoform 1 and in leukocytes, liver, prostate and skeletal muscle for isoform 2.

Inductioni

By TNF, IFNG/IFN-gamma and IFNB1/IFN-beta.2 Publications

Gene expression databases

BgeeiENSG00000013374.
CleanExiHS_NUB1.

Organism-specific databases

HPAiHPA021220.
HPA050722.
HPA053648.

Interactioni

Subunit structurei

Directly interacts with NEDD8 and PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome. Isoform 1 binds to NEDD8 more efficiently than isoform 2. Interacts with AIPL1. The interaction with UBD via UBA domains facilitates the linking of UBD-conjugated target protein to the proteasome complex and accelerates UBD degradation and that of its conjugates.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AIPL1Q9NZN92EBI-3936907,EBI-6557414
SF3B4Q154273EBI-3936907,EBI-348469

Protein-protein interaction databases

BioGridi119670. 24 interactors.
IntActiQ9Y5A7. 7 interactors.
STRINGi9606.ENSP00000457628.

Structurei

Secondary structure

1615
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi76 – 78Combined sources3
Beta strandi82 – 88Combined sources7
Beta strandi96 – 108Combined sources13
Helixi109 – 119Combined sources11
Beta strandi128 – 131Combined sources4
Helixi142 – 145Combined sources4
Beta strandi149 – 156Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WJUNMR-A75-161[»]
ProteinModelPortaliQ9Y5A7.
SMRiQ9Y5A7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y5A7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini374 – 413UBA 1PROSITE-ProRule annotationAdd BLAST40
Domaini424 – 470UBA 2PROSITE-ProRule annotationAdd BLAST47
Domaini489 – 529UBA 3PROSITE-ProRule annotationAdd BLAST41

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni427 – 474NEDD8-binding 1Add BLAST48
Regioni550 – 598NEDD8-binding 2Add BLAST49

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili36 – 70Sequence analysisAdd BLAST35
Coiled coili152 – 203Sequence analysisAdd BLAST52

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi414 – 431Nuclear localization signalCuratedAdd BLAST18

Sequence similaritiesi

Contains 3 UBA domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG2561. Eukaryota.
ENOG410ZK3P. LUCA.
HOGENOMiHOG000113882.
HOVERGENiHBG045600.
InParanoidiQ9Y5A7.
PhylomeDBiQ9Y5A7.
TreeFamiTF323449.

Family and domain databases

InterProiIPR011990. TPR-like_helical_dom.
IPR015940. UBA.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00627. UBA. 2 hits.
[Graphical view]
SMARTiSM00165. UBA. 3 hits.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 3 hits.
SSF48452. SSF48452. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50030. UBA. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y5A7-1) [UniParc]FASTAAdd to basket
Also known as: NUB1L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQKKYLQAK LTQFLREDRI QLWKPPYTDE NKKVGLALKD LAKQYSDRLE
60 70 80 90 100
CCENEVEKVI EEIRCKAIER GTGNDNYRTT GIATIEVFLP PRLKKDRKNL
110 120 130 140 150
LETRLHITGR ELRSKIAETF GLQENYIKIV INKKQLQLGK TLEEQGVAHN
160 170 180 190 200
VKAMVLELKQ SEEDARKNFQ LEEEEQNEAK LKEKQIQRTK RGLEILAKRA
210 220 230 240 250
AETVVDPEMT PYLDIANQTG RSIRIPPSER KALMLAMGYH EKGRAFLKRK
260 270 280 290 300
EYGIALPCLL DADKYFCECC RELLDTVDNY AVLQLDIVWC YFRLEQLECL
310 320 330 340 350
DDAEKKLNLA QKCFKNCYGE NHQRLVHIKG NCGKEKVLFL RLYLLQGIRN
360 370 380 390 400
YHSGNDVEAY EYLNKARQLF KELYIDPSKV DNLLQLGFTA QEARLGLRAC
410 420 430 440 450
DGNVDHAATH ITNRREELAQ IRKEEKEKKR RRLENIRFLK GMGYSTHAAQ
460 470 480 490 500
QVLHAASGNL DEALKILLSN PQMWWLNDSN PETDNRQESP SQENIDRLVY
510 520 530 540 550
MGFDALVAEA ALRVFRGNVQ LAAQTLAHNG GSLPPELPLS PEDSLSPPAT
560 570 580 590 600
SPSDSAGTSS ASTDEDMETE AVNEILEDIP EHEEDYLDST LEDEEIIIAE
610
YLSYVENRKS ATKKN
Length:615
Mass (Da):70,538
Last modified:September 26, 2003 - v2
Checksum:iD70FD6B21F4CD42E
GO
Isoform 2 (identifier: Q9Y5A7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     452-465: Missing.

Show »
Length:601
Mass (Da):69,119
Checksum:i05F92632C2FCB547
GO

Sequence cautioni

The sequence AAD42865 differs from that shown. Reason: Frameshift at positions 365 and 393.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05736913Q → R.Corresponds to variant rs2302131dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008335452 – 465Missing in isoform 2. 4 PublicationsAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155099 mRNA. Translation: AAD42865.1. Frameshift.
AF300717 mRNA. Translation: AAK21001.1.
AF459743 mRNA. Translation: AAO14547.1.
AY129295 mRNA. Translation: AAN01355.1.
AC005486 Genomic DNA. Translation: AAS02030.1.
BC046354 mRNA. Translation: AAH46354.1.
AF108083 Genomic DNA. Translation: AAC82474.2.
RefSeqiNP_001230280.1. NM_001243351.1.
NP_057202.3. NM_016118.4.
XP_005250068.1. XM_005250011.2. [Q9Y5A7-1]
XP_016867795.1. XM_017012306.1. [Q9Y5A7-2]
UniGeneiHs.647082.

Genome annotation databases

EnsembliENST00000355851; ENSP00000348110; ENSG00000013374.
ENST00000566856; ENSP00000457628; ENSG00000013374.
GeneIDi51667.
KEGGihsa:51667.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155099 mRNA. Translation: AAD42865.1. Frameshift.
AF300717 mRNA. Translation: AAK21001.1.
AF459743 mRNA. Translation: AAO14547.1.
AY129295 mRNA. Translation: AAN01355.1.
AC005486 Genomic DNA. Translation: AAS02030.1.
BC046354 mRNA. Translation: AAH46354.1.
AF108083 Genomic DNA. Translation: AAC82474.2.
RefSeqiNP_001230280.1. NM_001243351.1.
NP_057202.3. NM_016118.4.
XP_005250068.1. XM_005250011.2. [Q9Y5A7-1]
XP_016867795.1. XM_017012306.1. [Q9Y5A7-2]
UniGeneiHs.647082.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WJUNMR-A75-161[»]
ProteinModelPortaliQ9Y5A7.
SMRiQ9Y5A7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119670. 24 interactors.
IntActiQ9Y5A7. 7 interactors.
STRINGi9606.ENSP00000457628.

PTM databases

iPTMnetiQ9Y5A7.
PhosphoSitePlusiQ9Y5A7.

Polymorphism and mutation databases

BioMutaiNUB1.
DMDMi37154888.

Proteomic databases

PaxDbiQ9Y5A7.
PeptideAtlasiQ9Y5A7.
PRIDEiQ9Y5A7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000355851; ENSP00000348110; ENSG00000013374.
ENST00000566856; ENSP00000457628; ENSG00000013374.
GeneIDi51667.
KEGGihsa:51667.

Organism-specific databases

CTDi51667.
DisGeNETi51667.
GeneCardsiNUB1.
H-InvDBHIX0019486.
HGNCiHGNC:17623. NUB1.
HPAiHPA021220.
HPA050722.
HPA053648.
MIMi607981. gene.
neXtProtiNX_Q9Y5A7.
PharmGKBiPA147357533.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2561. Eukaryota.
ENOG410ZK3P. LUCA.
HOGENOMiHOG000113882.
HOVERGENiHBG045600.
InParanoidiQ9Y5A7.
PhylomeDBiQ9Y5A7.
TreeFamiTF323449.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000013374-MONOMER.
ZFISH:ENSG00000103274-MONOMER.

Miscellaneous databases

ChiTaRSiNUB1. human.
EvolutionaryTraceiQ9Y5A7.
GeneWikiiNUB1.
GenomeRNAii51667.
PROiQ9Y5A7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000013374.
CleanExiHS_NUB1.

Family and domain databases

InterProiIPR011990. TPR-like_helical_dom.
IPR015940. UBA.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00627. UBA. 2 hits.
[Graphical view]
SMARTiSM00165. UBA. 3 hits.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 3 hits.
SSF48452. SSF48452. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50030. UBA. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUB1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y5A7
Secondary accession number(s): O95422
, Q75MR9, Q8IX22, Q9BXR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: September 26, 2003
Last modified: November 2, 2016
This is version 158 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.