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Q9Y5A7 (NUB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NEDD8 ultimate buster 1
Alternative name(s):
Negative regulator of ubiquitin-like proteins 1
Renal carcinoma antigen NY-REN-18
Gene names
Name:NUB1
Synonyms:NYREN18
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length615 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2. Ref.10

Subunit structure

Directly interacts with NEDD8 and PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome. Isoform 1 binds to NEDD8 more efficiently than isoform 2. Interacts with AIPL1. The interaction with UBD via UBA domains facilitates the linking of UBD-conjugated target protein to the proteasome complex and accelerates UBD degradation and that of its conjugates. Ref.2 Ref.7 Ref.8 Ref.9 Ref.10

Subcellular location

Nucleus. Note: Predominantly nuclear. Ref.2

Tissue specificity

Widely expressed with lowest expression in the pancreas for isoform 1 and in leukocytes, liver, prostate and skeletal muscle for isoform 2.

Induction

By TNF, IFNG/IFN-gamma and IFNB1/IFN-beta. Ref.2 Ref.10

Sequence similarities

Contains 3 UBA domains.

Sequence caution

The sequence AAD42865.1 differs from that shown. Reason: Frameshift at positions 365 and 393.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

AIPL1Q9NZN92EBI-3936907,EBI-6557414

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Y5A7-1)

Also known as: NUB1L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Y5A7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     452-465: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 615615NEDD8 ultimate buster 1
PRO_0000210992

Regions

Domain374 – 41340UBA 1
Domain424 – 47047UBA 2
Domain489 – 52941UBA 3
Region427 – 47448NEDD8-binding 1
Region550 – 59849NEDD8-binding 2
Coiled coil36 – 7035 Potential
Coiled coil152 – 20352 Potential
Motif414 – 43118Nuclear localization signal Probable

Natural variations

Alternative sequence452 – 46514Missing in isoform 2.
VSP_008335
Natural variant131Q → R.
Corresponds to variant rs2302131 [ dbSNP | Ensembl ].
VAR_057369

Experimental info

Mutagenesis4481A → V: No effect on NEDD8-binding. Ref.3
Mutagenesis4531L → A: Partial inhibition of NEDD8-binding. Ref.3
Mutagenesis4641L → A: Partial inhibition of NEDD8-binding. Ref.3
Mutagenesis4681L → A: Partial inhibition of NEDD8-binding. Ref.3
Mutagenesis5871L → A: Suppression of NEDD8-binding; when associated with A-464; A-468 and A-591. Suppression of NEDD8-buster function; when associated with A-591. Ref.3
Mutagenesis5911L → A: Suppression of NEDD8-binding; when associated with A-464; A-468 and A-587. Suppression of NEDD8-buster function; when associated with A-587. Ref.3

Secondary structure

.............. 615
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (NUB1L) [UniParc].

Last modified September 26, 2003. Version 2.
Checksum: D70FD6B21F4CD42E

FASTA61570,538
        10         20         30         40         50         60 
MAQKKYLQAK LTQFLREDRI QLWKPPYTDE NKKVGLALKD LAKQYSDRLE CCENEVEKVI 

        70         80         90        100        110        120 
EEIRCKAIER GTGNDNYRTT GIATIEVFLP PRLKKDRKNL LETRLHITGR ELRSKIAETF 

       130        140        150        160        170        180 
GLQENYIKIV INKKQLQLGK TLEEQGVAHN VKAMVLELKQ SEEDARKNFQ LEEEEQNEAK 

       190        200        210        220        230        240 
LKEKQIQRTK RGLEILAKRA AETVVDPEMT PYLDIANQTG RSIRIPPSER KALMLAMGYH 

       250        260        270        280        290        300 
EKGRAFLKRK EYGIALPCLL DADKYFCECC RELLDTVDNY AVLQLDIVWC YFRLEQLECL 

       310        320        330        340        350        360 
DDAEKKLNLA QKCFKNCYGE NHQRLVHIKG NCGKEKVLFL RLYLLQGIRN YHSGNDVEAY 

       370        380        390        400        410        420 
EYLNKARQLF KELYIDPSKV DNLLQLGFTA QEARLGLRAC DGNVDHAATH ITNRREELAQ 

       430        440        450        460        470        480 
IRKEEKEKKR RRLENIRFLK GMGYSTHAAQ QVLHAASGNL DEALKILLSN PQMWWLNDSN 

       490        500        510        520        530        540 
PETDNRQESP SQENIDRLVY MGFDALVAEA ALRVFRGNVQ LAAQTLAHNG GSLPPELPLS 

       550        560        570        580        590        600 
PEDSLSPPAT SPSDSAGTSS ASTDEDMETE AVNEILEDIP EHEEDYLDST LEDEEIIIAE 

       610 
YLSYVENRKS ATKKN 

« Hide

Isoform 2 [UniParc].

Checksum: 05F92632C2FCB547
Show »

FASTA60169,119

References

« Hide 'large scale' references
[1]"Antigens recognized by autologous antibody in patients with renal-cell carcinoma."
Scanlan M.J., Gordan J.D., Williamson B., Stockert E., Bander N.H., Jongeneel C.V., Gure A.O., Jaeger D., Jaeger E., Knuth A., Chen Y.-T., Old L.J.
Int. J. Cancer 83:456-464(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), IDENTIFICATION AS A RENAL CANCER ANTIGEN.
Tissue: Renal cell carcinoma.
[2]"NUB1, a NEDD8-interacting protein, is induced by interferon and down-regulates the NEDD8 expression."
Kito K., Yeh E.T.H., Kamitani T.
J. Biol. Chem. 276:20603-20609(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INTERACTION WITH NEDD8, SUBCELLULAR LOCATION, INDUCTION BY INTERFERONS.
Tissue: Heart.
[3]"Regulation of the NEDD8 conjugation system by a splicing variant, NUB1L."
Tanaka T., Kawashima H., Yeh E.T.H., Kamitani T.
J. Biol. Chem. 278:32905-32913(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), CHARACTERIZATION, MUTAGENESIS OF ALA-448; LEU-453; LEU-464; LEU-468; LEU-587 AND LEU-591.
Tissue: Testis.
[4]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Testis.
[6]Rump A., Rosenthal A., Drescher B., Weber J., Schattevoy R., Korenberg J.
Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-365.
[7]"Targeting of NEDD8 and its conjugates for proteasomal degradation by NUB1."
Kamitani T., Kito K., Fukuda-Kamitani T., Yeh E.T.H.
J. Biol. Chem. 276:46655-46660(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PSMD4.
[8]"The inherited blindness associated protein AIPL1 interacts with the cell cycle regulator protein NUB1."
Akey D.T., Zhu X., Dyer M., Li A., Sorensen A., Blackshaw S., Fukuda-Kamitani T., Daiger S.P., Craft C.M., Kamitani T., Sohocki M.M.
Hum. Mol. Genet. 11:2723-2733(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH AIPL1.
Tissue: Retina.
[9]"NEDD8 ultimate buster-1L interacts with the ubiquitin-like protein FAT10 and accelerates its degradation."
Hipp M.S., Raasi S., Groettrup M., Schmidtke G.
J. Biol. Chem. 279:16503-16510(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UBD.
[10]"The UBA domains of NUB1L are required for binding but not for accelerated degradation of the ubiquitin-like modifier FAT10."
Schmidtke G., Kalveram B., Weber E., Bochtler P., Lukasiak S., Hipp M.S., Groettrup M.
J. Biol. Chem. 281:20045-20054(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH UBD AND PROTEASOME, INDUCTION BY TNF AND IFNG.
[11]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Solution structure of N-terminal ubiquitin-like domain of human NEDD8 ultimate buster-1."
RIKEN structural genomics initiative (RSGI)
Submitted (JAN-2006) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 75-161.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF155099 mRNA. Translation: AAD42865.1. Frameshift.
AF300717 mRNA. Translation: AAK21001.1.
AF459743 mRNA. Translation: AAO14547.1.
AY129295 mRNA. Translation: AAN01355.1.
AC005486 Genomic DNA. Translation: AAS02030.1.
BC046354 mRNA. Translation: AAH46354.1.
AF108083 Genomic DNA. Translation: AAC82474.2.
RefSeqNP_001230280.1. NM_001243351.1.
NP_057202.3. NM_016118.4.
XP_005250068.1. XM_005250011.1.
UniGeneHs.647082.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1WJUNMR-A75-161[»]
ProteinModelPortalQ9Y5A7.
SMRQ9Y5A7. Positions 71-161, 374-544.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119670. 156 interactions.
IntActQ9Y5A7. 4 interactions.
STRING9606.ENSP00000398644.

PTM databases

PhosphoSiteQ9Y5A7.

Polymorphism databases

DMDM37154888.

Proteomic databases

PaxDbQ9Y5A7.
PRIDEQ9Y5A7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000355851; ENSP00000348110; ENSG00000013374. [Q9Y5A7-1]
ENST00000566856; ENSP00000457628; ENSG00000013374. [Q9Y5A7-2]
GeneID51667.
KEGGhsa:51667.

Organism-specific databases

CTD51667.
GeneCardsGC07P151038.
H-InvDBHIX0019486.
HGNCHGNC:17623. NUB1.
HPAHPA021220.
HPA050722.
HPA053648.
MIM607981. gene.
neXtProtNX_Q9Y5A7.
PharmGKBPA147357533.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG302480.
HOGENOMHOG000113882.
HOVERGENHBG045600.
InParanoidQ9Y5A7.
PhylomeDBQ9Y5A7.
TreeFamTF323449.

Gene expression databases

ArrayExpressQ9Y5A7.
BgeeQ9Y5A7.
CleanExHS_NUB1.
GenevestigatorQ9Y5A7.

Family and domain databases

InterProIPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
[Graphical view]
PfamPF00627. UBA. 1 hit.
[Graphical view]
SMARTSM00165. UBA. 3 hits.
[Graphical view]
SUPFAMSSF46934. SSF46934. 3 hits.
PROSITEPS50030. UBA. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNUB1. human.
EvolutionaryTraceQ9Y5A7.
GeneWikiNUB1.
GenomeRNAi51667.
NextBio55660.
PROQ9Y5A7.
SOURCESearch...

Entry information

Entry nameNUB1_HUMAN
AccessionPrimary (citable) accession number: Q9Y5A7
Secondary accession number(s): O95422 expand/collapse secondary AC list , Q75MR9, Q8IX22, Q9BXR2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: September 26, 2003
Last modified: April 16, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM