Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9Y5A7

- NUB1_HUMAN

UniProt

Q9Y5A7 - NUB1_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

NEDD8 ultimate buster 1

Gene

NUB1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2.1 Publication

GO - Biological processi

  1. positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
  2. protein ubiquitination Source: UniProtKB
  3. response to interferon-gamma Source: UniProtKB
  4. response to tumor necrosis factor Source: UniProtKB
  5. ubiquitin-dependent protein catabolic process Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8 ultimate buster 1
Alternative name(s):
Negative regulator of ubiquitin-like proteins 1
Renal carcinoma antigen NY-REN-18
Gene namesi
Name:NUB1
Synonyms:NYREN18
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 7

Organism-specific databases

HGNCiHGNC:17623. NUB1.

Subcellular locationi

Nucleus 1 Publication
Note: Predominantly nuclear.

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. nucleolus Source: HPA
  3. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi448 – 4481A → V: No effect on NEDD8-binding. 1 Publication
Mutagenesisi453 – 4531L → A: Partial inhibition of NEDD8-binding. 1 Publication
Mutagenesisi464 – 4641L → A: Partial inhibition of NEDD8-binding. 1 Publication
Mutagenesisi468 – 4681L → A: Partial inhibition of NEDD8-binding. 1 Publication
Mutagenesisi587 – 5871L → A: Suppression of NEDD8-binding; when associated with A-464; A-468 and A-591. Suppression of NEDD8-buster function; when associated with A-591. 1 Publication
Mutagenesisi591 – 5911L → A: Suppression of NEDD8-binding; when associated with A-464; A-468 and A-587. Suppression of NEDD8-buster function; when associated with A-587. 1 Publication

Organism-specific databases

PharmGKBiPA147357533.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 615615NEDD8 ultimate buster 1PRO_0000210992Add
BLAST

Proteomic databases

MaxQBiQ9Y5A7.
PaxDbiQ9Y5A7.
PRIDEiQ9Y5A7.

PTM databases

PhosphoSiteiQ9Y5A7.

Expressioni

Tissue specificityi

Widely expressed with lowest expression in the pancreas for isoform 1 and in leukocytes, liver, prostate and skeletal muscle for isoform 2.

Inductioni

By TNF, IFNG/IFN-gamma and IFNB1/IFN-beta.2 Publications

Gene expression databases

BgeeiQ9Y5A7.
CleanExiHS_NUB1.
ExpressionAtlasiQ9Y5A7. baseline and differential.
GenevestigatoriQ9Y5A7.

Organism-specific databases

HPAiHPA021220.
HPA050722.
HPA053648.

Interactioni

Subunit structurei

Directly interacts with NEDD8 and PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome. Isoform 1 binds to NEDD8 more efficiently than isoform 2. Interacts with AIPL1. The interaction with UBD via UBA domains facilitates the linking of UBD-conjugated target protein to the proteasome complex and accelerates UBD degradation and that of its conjugates.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AIPL1Q9NZN92EBI-3936907,EBI-6557414

Protein-protein interaction databases

BioGridi119670. 157 interactions.
IntActiQ9Y5A7. 4 interactions.
STRINGi9606.ENSP00000398644.

Structurei

Secondary structure

1
615
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi76 – 783
Beta strandi82 – 887
Beta strandi96 – 10813
Helixi109 – 11911
Beta strandi128 – 1314
Helixi142 – 1454
Beta strandi149 – 1568

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WJUNMR-A75-161[»]
ProteinModelPortaliQ9Y5A7.
SMRiQ9Y5A7. Positions 71-161, 470-544.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y5A7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini374 – 41340UBA 1PROSITE-ProRule annotationAdd
BLAST
Domaini424 – 47047UBA 2PROSITE-ProRule annotationAdd
BLAST
Domaini489 – 52941UBA 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni427 – 47448NEDD8-binding 1Add
BLAST
Regioni550 – 59849NEDD8-binding 2Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili36 – 7035Sequence AnalysisAdd
BLAST
Coiled coili152 – 20352Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi414 – 43118Nuclear localization signalCuratedAdd
BLAST

Sequence similaritiesi

Contains 3 UBA domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiNOG302480.
HOGENOMiHOG000113882.
HOVERGENiHBG045600.
InParanoidiQ9Y5A7.
PhylomeDBiQ9Y5A7.
TreeFamiTF323449.

Family and domain databases

InterProiIPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM00165. UBA. 3 hits.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 3 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50030. UBA. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9Y5A7-1) [UniParc]FASTAAdd to Basket

Also known as: NUB1L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQKKYLQAK LTQFLREDRI QLWKPPYTDE NKKVGLALKD LAKQYSDRLE
60 70 80 90 100
CCENEVEKVI EEIRCKAIER GTGNDNYRTT GIATIEVFLP PRLKKDRKNL
110 120 130 140 150
LETRLHITGR ELRSKIAETF GLQENYIKIV INKKQLQLGK TLEEQGVAHN
160 170 180 190 200
VKAMVLELKQ SEEDARKNFQ LEEEEQNEAK LKEKQIQRTK RGLEILAKRA
210 220 230 240 250
AETVVDPEMT PYLDIANQTG RSIRIPPSER KALMLAMGYH EKGRAFLKRK
260 270 280 290 300
EYGIALPCLL DADKYFCECC RELLDTVDNY AVLQLDIVWC YFRLEQLECL
310 320 330 340 350
DDAEKKLNLA QKCFKNCYGE NHQRLVHIKG NCGKEKVLFL RLYLLQGIRN
360 370 380 390 400
YHSGNDVEAY EYLNKARQLF KELYIDPSKV DNLLQLGFTA QEARLGLRAC
410 420 430 440 450
DGNVDHAATH ITNRREELAQ IRKEEKEKKR RRLENIRFLK GMGYSTHAAQ
460 470 480 490 500
QVLHAASGNL DEALKILLSN PQMWWLNDSN PETDNRQESP SQENIDRLVY
510 520 530 540 550
MGFDALVAEA ALRVFRGNVQ LAAQTLAHNG GSLPPELPLS PEDSLSPPAT
560 570 580 590 600
SPSDSAGTSS ASTDEDMETE AVNEILEDIP EHEEDYLDST LEDEEIIIAE
610
YLSYVENRKS ATKKN
Length:615
Mass (Da):70,538
Last modified:September 26, 2003 - v2
Checksum:iD70FD6B21F4CD42E
GO
Isoform 2 (identifier: Q9Y5A7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     452-465: Missing.

Show »
Length:601
Mass (Da):69,119
Checksum:i05F92632C2FCB547
GO

Sequence cautioni

The sequence AAD42865.1 differs from that shown. Reason: Frameshift at positions 365 and 393.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti13 – 131Q → R.
Corresponds to variant rs2302131 [ dbSNP | Ensembl ].
VAR_057369

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei452 – 46514Missing in isoform 2. 4 PublicationsVSP_008335Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF155099 mRNA. Translation: AAD42865.1. Frameshift.
AF300717 mRNA. Translation: AAK21001.1.
AF459743 mRNA. Translation: AAO14547.1.
AY129295 mRNA. Translation: AAN01355.1.
AC005486 Genomic DNA. Translation: AAS02030.1.
BC046354 mRNA. Translation: AAH46354.1.
AF108083 Genomic DNA. Translation: AAC82474.2.
RefSeqiNP_001230280.1. NM_001243351.1.
NP_057202.3. NM_016118.4.
XP_005250068.1. XM_005250011.1. [Q9Y5A7-1]
UniGeneiHs.647082.

Genome annotation databases

EnsembliENST00000355851; ENSP00000348110; ENSG00000013374.
ENST00000566856; ENSP00000457628; ENSG00000013374.
GeneIDi51667.
KEGGihsa:51667.

Polymorphism databases

DMDMi37154888.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF155099 mRNA. Translation: AAD42865.1 . Frameshift.
AF300717 mRNA. Translation: AAK21001.1 .
AF459743 mRNA. Translation: AAO14547.1 .
AY129295 mRNA. Translation: AAN01355.1 .
AC005486 Genomic DNA. Translation: AAS02030.1 .
BC046354 mRNA. Translation: AAH46354.1 .
AF108083 Genomic DNA. Translation: AAC82474.2 .
RefSeqi NP_001230280.1. NM_001243351.1.
NP_057202.3. NM_016118.4.
XP_005250068.1. XM_005250011.1. [Q9Y5A7-1 ]
UniGenei Hs.647082.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1WJU NMR - A 75-161 [» ]
ProteinModelPortali Q9Y5A7.
SMRi Q9Y5A7. Positions 71-161, 470-544.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 119670. 157 interactions.
IntActi Q9Y5A7. 4 interactions.
STRINGi 9606.ENSP00000398644.

PTM databases

PhosphoSitei Q9Y5A7.

Polymorphism databases

DMDMi 37154888.

Proteomic databases

MaxQBi Q9Y5A7.
PaxDbi Q9Y5A7.
PRIDEi Q9Y5A7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000355851 ; ENSP00000348110 ; ENSG00000013374 .
ENST00000566856 ; ENSP00000457628 ; ENSG00000013374 .
GeneIDi 51667.
KEGGi hsa:51667.

Organism-specific databases

CTDi 51667.
GeneCardsi GC07P151038.
H-InvDB HIX0019486.
HGNCi HGNC:17623. NUB1.
HPAi HPA021220.
HPA050722.
HPA053648.
MIMi 607981. gene.
neXtProti NX_Q9Y5A7.
PharmGKBi PA147357533.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG302480.
HOGENOMi HOG000113882.
HOVERGENi HBG045600.
InParanoidi Q9Y5A7.
PhylomeDBi Q9Y5A7.
TreeFami TF323449.

Miscellaneous databases

ChiTaRSi NUB1. human.
EvolutionaryTracei Q9Y5A7.
GeneWikii NUB1.
GenomeRNAii 51667.
NextBioi 55660.
PROi Q9Y5A7.
SOURCEi Search...

Gene expression databases

Bgeei Q9Y5A7.
CleanExi HS_NUB1.
ExpressionAtlasi Q9Y5A7. baseline and differential.
Genevestigatori Q9Y5A7.

Family and domain databases

InterProi IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
Pfami PF00627. UBA. 1 hit.
[Graphical view ]
SMARTi SM00165. UBA. 3 hits.
[Graphical view ]
SUPFAMi SSF46934. SSF46934. 3 hits.
SSF54236. SSF54236. 1 hit.
PROSITEi PS50030. UBA. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), IDENTIFICATION AS A RENAL CANCER ANTIGEN.
    Tissue: Renal cell carcinoma.
  2. "NUB1, a NEDD8-interacting protein, is induced by interferon and down-regulates the NEDD8 expression."
    Kito K., Yeh E.T.H., Kamitani T.
    J. Biol. Chem. 276:20603-20609(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INTERACTION WITH NEDD8, SUBCELLULAR LOCATION, INDUCTION BY INTERFERONS.
    Tissue: Heart.
  3. "Regulation of the NEDD8 conjugation system by a splicing variant, NUB1L."
    Tanaka T., Kawashima H., Yeh E.T.H., Kamitani T.
    J. Biol. Chem. 278:32905-32913(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), CHARACTERIZATION, MUTAGENESIS OF ALA-448; LEU-453; LEU-464; LEU-468; LEU-587 AND LEU-591.
    Tissue: Testis.
  4. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.
  6. Rump A., Rosenthal A., Drescher B., Weber J., Schattevoy R., Korenberg J.
    Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-365.
  7. "Targeting of NEDD8 and its conjugates for proteasomal degradation by NUB1."
    Kamitani T., Kito K., Fukuda-Kamitani T., Yeh E.T.H.
    J. Biol. Chem. 276:46655-46660(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PSMD4.
  8. "The inherited blindness associated protein AIPL1 interacts with the cell cycle regulator protein NUB1."
    Akey D.T., Zhu X., Dyer M., Li A., Sorensen A., Blackshaw S., Fukuda-Kamitani T., Daiger S.P., Craft C.M., Kamitani T., Sohocki M.M.
    Hum. Mol. Genet. 11:2723-2733(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AIPL1.
    Tissue: Retina.
  9. "NEDD8 ultimate buster-1L interacts with the ubiquitin-like protein FAT10 and accelerates its degradation."
    Hipp M.S., Raasi S., Groettrup M., Schmidtke G.
    J. Biol. Chem. 279:16503-16510(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UBD.
  10. "The UBA domains of NUB1L are required for binding but not for accelerated degradation of the ubiquitin-like modifier FAT10."
    Schmidtke G., Kalveram B., Weber E., Bochtler P., Lukasiak S., Hipp M.S., Groettrup M.
    J. Biol. Chem. 281:20045-20054(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH UBD AND PROTEASOME, INDUCTION BY TNF AND IFNG.
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Solution structure of N-terminal ubiquitin-like domain of human NEDD8 ultimate buster-1."
    RIKEN structural genomics initiative (RSGI)
    Submitted (JAN-2006) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 75-161.

Entry informationi

Entry nameiNUB1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y5A7
Secondary accession number(s): O95422
, Q75MR9, Q8IX22, Q9BXR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: September 26, 2003
Last modified: October 29, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3