Q9Y581 (INSL6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Insulin-like peptide INSL6
Short name=Insulin-like peptide 6

Alternative name(s):
Relaxin/insulin-like factor 1

Cleaved into the following 2 chains:

1. Insulin-like peptide INSL6 B chain
2. Insulin-like peptide INSL6 A chain

Gene names

Name:	INSL6
Synonyms:	RIF1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May have a role in sperm development and fertilization.
Subcellular location	Secreted By similarity.
Tissue specificity	Testis specific.
Sequence similarities	Belongs to the insulin family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Hormone
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: Compara fertilization Inferred from electronic annotation. Source: Compara male gonad development Inferred from electronic annotation. Source: Compara multicellular organism growth Inferred from electronic annotation. Source: Compara pachytene Inferred from electronic annotation. Source: Compara sperm motility Inferred from electronic annotation. Source: Compara spermatid development Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hormone activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 213	193	Insulin-like peptide INSL6		PRO_0000016172
	Peptide	21 – 53	33	Insulin-like peptide INSL6 B chain Potential		PRO_0000016173
	Propeptide	55 – 168	114	Connecting peptide Potential		PRO_0000016174
	Peptide	173 – 198	26	Insulin-like peptide INSL6 A chain Potential		PRO_0000016175
	Propeptide	201 – 213	13	Potential		PRO_0000016176
Amino acid modifications
	Disulfide bond	33 ↔ 179		By similarity
	Disulfide bond	45 ↔ 192		By similarity
	Disulfide bond	178 ↔ 183		By similarity
Natural variations
	Natural variant	80	1	F → L. Corresponds to variant rs2149554 [ dbSNP | Ensembl ].		VAR_024329
Experimental info
	Sequence conflict	146	1	K → F in AAD39003. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9Y581 [UniParc]. Last modified April 16, 2002. Version 2. Checksum: 12A097659CFAC471	FASTA	213	24,860
	10 20 30 40 50 60 MPRLLRLSLL WLGLLLVRFS RELSDISSAR KLCGRYLVKE IEKLCGHANW SQFRFEEETP 70 80 90 100 110 120 FSRLIAQASE KVEAYSPYQF ESPQTASPAR GRGTNPVSTS WEEAVNSWEM QSLPEYKDKK 130 140 150 160 170 180 GYSPLGKTRE FSSSHNINVY IHENAKFQKK RRNKIKTLSN LFWGHHPQRK RRGYSEKCCL 190 200 210 TGCTKEELSI ACLPYIDFKR LKEKRSSLVT KIY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of two novel mammalian paralogs of relaxin/insulin family proteins and their expression in testis and kidney." Hsu S.Y. Mol. Endocrinol. 13:2163-2174(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	"Identification of INSL6, a new member of the insulin family that is expressed in the testis of the human and Rat." Lok S., Johnston D.S., Conklin D., Lofton-Day C.E., Adams R.L., Jelmberg A.C., Whitmore T.E., Schrader S., Griswold M.D., Jaspers S.R. Biol. Reprod. 62:1593-1599(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[3]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF135824 mRNA. Translation: AAF29604.1. AF156094 mRNA. Translation: AAD39003.1. AL161450 Genomic DNA. Translation: CAD13336.1. BC126473 mRNA. Translation: AAI26474.1. BC126475 mRNA. Translation: AAI26476.1.
IPI	IPI00294949.
RefSeq	NP_009110.2. NM_007179.2.
UniGene	Hs.632648.
3D structure databases
ProteinModelPortal	Q9Y581.
SMR	Q9Y581. Positions 26-52.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000371054.
PTM databases
PhosphoSite	Q9Y581.
Polymorphism databases
DMDM	20178309.
Proteomic databases
PaxDb	Q9Y581.
PRIDE	Q9Y581.
Protocols and materials databases
DNASU	11172.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000381641; ENSP00000371054; ENSG00000120210.
GeneID	11172.
KEGG	hsa:11172.
UCSC	uc003zix.3. human.
Organism-specific databases
CTD	11172.
GeneCards	GC09M005163.
HGNC	HGNC:6089. INSL6.
HPA	HPA021364.
MIM	606414. gene.
neXtProt	NX_Q9Y581.
PharmGKB	PA29896.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG47664.
HOGENOM	HOG000113078.
HOVERGEN	HBG052136.
InParanoid	Q9Y581.
KO	K05255.
OMA	FWGNHPQ.
OrthoDB	EOG447FVJ.
PhylomeDB	Q9Y581.
Gene expression databases
Bgee	Q9Y581.
CleanEx	HS_INSL6. HS_RIF1.
Genevestigator	Q9Y581.
GermOnline	ENSG00000120210. Homo sapiens.
Family and domain databases
Gene3D	1.10.100.10. 2 hits.
InterPro	IPR016179. Insulin-like. IPR017100. Insulin-like_pep_6. IPR022353. Insulin_CS. [Graphical view]
PANTHER	PTHR12004:SF1. PTHR12004:SF1. 1 hit.
Pfam	PF00049. Insulin. 1 hit. [Graphical view]
PIRSF	PIRSF037062. Insulin-like_peptide_6. 1 hit.
SMART	SM00078. IlGF. 1 hit. [Graphical view]
SUPFAM	SSF56994. Insulin-like. 1 hit.
PROSITE	PS00262. INSULIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	11172.
NextBio	42513.
SOURCE	Search...

Entry information

Entry name

INSL6_HUMAN

Accession

Primary (citable) accession number: Q9Y581
Secondary accession number(s): A0AVS0, Q9NS16

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	April 16, 2002
Last modified:	April 3, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.