Q9Y575 (ASB3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ankyrin repeat and SOCS box protein 3
Short name=ASB-3

Gene names

Name:

ASB3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	518 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes TNFRSF1B. Ref.6
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with TCEB2 and TNFRSF1B. Ref.6
Domain	The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin-protein ligase complexes By similarity.
Sequence similarities	Contains 11 ANK repeats. Contains 1 SOCS box domain.
Sequence caution	The sequence AAI10916.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Domain	ANK repeat Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y575-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y575-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9Y575-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MAMMPLVLHHPERQAELGSGLRDGGGAALRPPGRLVKQM

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 518	518	Ankyrin repeat and SOCS box protein 3		PRO_0000066926
Regions
	Repeat	9 – 38	30	ANK 1
	Repeat	42 – 71	30	ANK 2
	Repeat	78 – 107	30	ANK 3
	Repeat	111 – 140	30	ANK 4
	Repeat	145 – 174	30	ANK 5
	Repeat	178 – 207	30	ANK 6
	Repeat	211 – 240	30	ANK 7
	Repeat	246 – 275	30	ANK 8
	Repeat	279 – 308	30	ANK 9
	Repeat	315 – 346	32	ANK 10
	Repeat	348 – 373	26	ANK 11
	Domain	441 – 504	64	SOCS box
Natural variations
	Alternative sequence	1 – 73	73	Missing in isoform 2.		VSP_036392
	Alternative sequence	1	1	M → MAMMPLVLHHPERQAELGSG LRDGGGAALRPPGRLVKQM in isoform 3.		VSP_044606
Experimental info
	Sequence conflict	118	1	L → S in BAA91599. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: E6FC2EF76DD814DF	FASTA	518	57,745
	10 20 30 40 50 60 MDFTEAYADT CSTVGLAARE GNVKVLRKLL KKGRSVDVAD NRGWMPIHEA AYHNSVECLQ 70 80 90 100 110 120 MLINADSSEN YIKMKTFEGF CALHLAASQG HWKIVQILLE AGADPNATTL EETTPLFLAV 130 140 150 160 170 180 ENGQIDVLRL LLQHGANVNG SHSMCGWNSL HQASFQENAE IIKLLLRKGA NKECQDDFGI 190 200 210 220 230 240 TPLFVAAQYG KLESLSILIS SGANVNCQAL DKATPLFIAA QEGHTKCVEL LLSSGADPDL 250 260 270 280 290 300 YCNEDSWQLP IHAAAQMGHT KILDLLIPLT NRACDTGLNK VSPVYSAVFG GHEDCLEILL 310 320 330 340 350 360 RNGYSPDAQA CLVFGFSSPV CMAFQKDCEF FGIVNILLKY GAQINELHLA YCLKYEKFSI 370 380 390 400 410 420 FRYFLRKGCS LGPWNHIYEF VNHAIKAQAK YKEWLPHLLV AGFDPLILLC NSWIDSVSID 430 440 450 460 470 480 TLIFTLEFTN WKTLAPAVER MLSARASNAW ILQQHIATVP SLTHLCRLEI RSSLKSERLR 490 500 510 SDSYISQLPL PRSLHNYLLY EDVLRMYEVP ELAAIQDG
	Isoform 2 [UniParc]. Checksum: DF2A006FEA3DD409	FASTA	445	49,617
	10 20 30 40 50 60 MKTFEGFCAL HLAASQGHWK IVQILLEAGA DPNATTLEET TPLFLAVENG QIDVLRLLLQ 70 80 90 100 110 120 HGANVNGSHS MCGWNSLHQA SFQENAEIIK LLLRKGANKE CQDDFGITPL FVAAQYGKLE 130 140 150 160 170 180 SLSILISSGA NVNCQALDKA TPLFIAAQEG HTKCVELLLS SGADPDLYCN EDSWQLPIHA 190 200 210 220 230 240 AAQMGHTKIL DLLIPLTNRA CDTGLNKVSP VYSAVFGGHE DCLEILLRNG YSPDAQACLV 250 260 270 280 290 300 FGFSSPVCMA FQKDCEFFGI VNILLKYGAQ INELHLAYCL KYEKFSIFRY FLRKGCSLGP 310 320 330 340 350 360 WNHIYEFVNH AIKAQAKYKE WLPHLLVAGF DPLILLCNSW IDSVSIDTLI FTLEFTNWKT 370 380 390 400 410 420 LAPAVERMLS ARASNAWILQ QHIATVPSLT HLCRLEIRSS LKSERLRSDS YISQLPLPRS 430 440 LHNYLLYEDV LRMYEVPELA AIQDG
	Isoform 3 [UniParc]. Checksum: A73962ECA9F739CD	FASTA	556	61,775
	10 20 30 40 50 60 MAMMPLVLHH PERQAELGSG LRDGGGAALR PPGRLVKQMD FTEAYADTCS TVGLAAREGN 70 80 90 100 110 120 VKVLRKLLKK GRSVDVADNR GWMPIHEAAY HNSVECLQML INADSSENYI KMKTFEGFCA 130 140 150 160 170 180 LHLAASQGHW KIVQILLEAG ADPNATTLEE TTPLFLAVEN GQIDVLRLLL QHGANVNGSH 190 200 210 220 230 240 SMCGWNSLHQ ASFQENAEII KLLLRKGANK ECQDDFGITP LFVAAQYGKL ESLSILISSG 250 260 270 280 290 300 ANVNCQALDK ATPLFIAAQE GHTKCVELLL SSGADPDLYC NEDSWQLPIH AAAQMGHTKI 310 320 330 340 350 360 LDLLIPLTNR ACDTGLNKVS PVYSAVFGGH EDCLEILLRN GYSPDAQACL VFGFSSPVCM 370 380 390 400 410 420 AFQKDCEFFG IVNILLKYGA QINELHLAYC LKYEKFSIFR YFLRKGCSLG PWNHIYEFVN 430 440 450 460 470 480 HAIKAQAKYK EWLPHLLVAG FDPLILLCNS WIDSVSIDTL IFTLEFTNWK TLAPAVERML 490 500 510 520 530 540 SARASNAWIL QQHIATVPSL THLCRLEIRS SLKSERLRSD SYISQLPLPR SLHNYLLYED 550 VLRMYEVPEL AAIQDG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the genes encoding the ankyrin repeat and SOCS box-containing proteins Asb-1, Asb-2, Asb-3 and Asb-4." Kile B.T., Viney E.M., Willson T.A., Brodnicki T.C., Cancilla M.R., Herlihy A.S., Croker B.A., Baca M., Nicola N.A., Hilton D.J., Alexander W.S. Gene 258:31-41(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Embryo and Teratocarcinoma.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Brain, Kidney and Lung.
[6]	"Ankyrin repeat and SOCS box 3 (ASB3) mediates ubiquitination and degradation of tumor necrosis factor receptor II." Chung A.S., Guan Y.J., Yuan Z.L., Albina J.E., Chin Y.E. Mol. Cell. Biol. 25:4716-4726(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH TCEB2 AND TNFRSF1B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF156778 mRNA. Translation: AAD41895.1. AK000985 mRNA. Translation: BAA91455.1. AK001283 mRNA. Translation: BAA91599.1. AK056069 mRNA. Translation: BAG51615.1. AC007883 Genomic DNA. Translation: AAY24350.1. AC008064 Genomic DNA. Translation: AAX93180.1. AC008068 Genomic DNA. No translation available. AC069157 Genomic DNA. No translation available. CH471053 Genomic DNA. Translation: EAX00167.1. CH471053 Genomic DNA. Translation: EAX00168.1. CH471053 Genomic DNA. Translation: EAX00169.1. CH471053 Genomic DNA. Translation: EAX00170.1. BC006488 mRNA. Translation: AAH06488.1. BC009569 mRNA. Translation: AAH09569.1. BC110915 mRNA. Translation: AAI10916.1. Different initiation.
IPI	IPI00007215. IPI00871755. IPI00921607.
RefSeq	NP_001157637.1. NM_001164165.1. NP_001188894.1. NM_001201965.1. NP_057199.1. NM_016115.4. NP_665862.1. NM_145863.2.
UniGene	Hs.40763.
3D structure databases
ProteinModelPortal	Q9Y575.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y575. 3 interactions.
STRING	9606.ENSP00000263634.
PTM databases
PhosphoSite	Q9Y575.
Polymorphism databases
DMDM	20532004.
Proteomic databases
PaxDb	Q9Y575.
PRIDE	Q9Y575.
Protocols and materials databases
DNASU	51130.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000263634; ENSP00000263634; ENSG00000115239. ENST00000352846; ENSP00000313756; ENSG00000115239. ENST00000394717; ENSP00000378206; ENSG00000115239. ENST00000406625; ENSP00000385085; ENSG00000115239. ENST00000406687; ENSP00000384728; ENSG00000115239.
GeneID	100302652. 51130.
KEGG	hsa:100302652. hsa:51130.
UCSC	uc002rxg.2. human.
Organism-specific databases
CTD	100302652. 51130.
GeneCards	GC02M053750. GC02M053759.
HGNC	HGNC:16013. ASB3.
HPA	HPA003940.
MIM	605760. gene.
neXtProt	NX_Q9Y575.
PharmGKB	PA25031.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0666.
HOVERGEN	HBG024356.
InParanoid	Q9Y575.
KO	K10325.
OMA	HLAYCLK.
OrthoDB	EOG4NVZK3.
PhylomeDB	Q9Y575.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q9Y575.
Bgee	Q9Y575.
CleanEx	HS_ASB3.
Genevestigator	Q9Y575.
GermOnline	ENSG00000115239. Homo sapiens.
Family and domain databases
Gene3D	1.25.40.20. 3 hits.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR001496. SOCS_C. [Graphical view]
Pfam	PF12796. Ank_2. 3 hits. PF07525. SOCS_box. 1 hit. [Graphical view]
PRINTS	PR01415. ANKYRIN.
SMART	SM00248. ANK. 10 hits. SM00969. SOCS_box. 1 hit. [Graphical view]
SUPFAM	SSF48403. ANK. 2 hits.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 6 hits. PS50225. SOCS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ASB3. human.
GenomeRNAi	51130.
NextBio	20796092.
SOURCE	Search...

Entry information

Entry name

ASB3_HUMAN

Accession

Primary (citable) accession number: Q9Y575
Secondary accession number(s): B3KPA3 , D6W5B4, D6W5B5, G8JLA9, Q2TAI4, Q53TN0, Q53TN8, Q9NVZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.