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Protein

Ankyrin repeat and SOCS box protein 4

Gene

ASB4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes differentiation and maturation of the vascular lineage by an oxygen-dependent mechanism (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei256Essential for interaction with HIF1ANBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciZFISH:ENSG00000005981-MONOMER.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ankyrin repeat and SOCS box protein 4
Short name:
ASB-4
Gene namesi
Name:ASB4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:16009. ASB4.

Subcellular locationi

GO - Cellular componenti

  • Cul2-RING ubiquitin ligase complex Source: UniProtKB
  • Cul5-RING ubiquitin ligase complex Source: UniProtKB
  • cytoplasm Source: GO_Central
  • nucleus Source: GO_Central
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi51666.
OpenTargetsiENSG00000005981.
PharmGKBiPA25032.

Polymorphism and mutation databases

BioMutaiASB4.
DMDMi20532003.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000669281 – 426Ankyrin repeat and SOCS box protein 4Add BLAST426

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei246(3S)-3-hydroxyasparagine; by HIF1ANBy similarity1

Post-translational modificationi

Hydroxylation at Asn-246 by HIF1AN may provide an oxygen-dependent regulation mechanism for the function of ASB4 in promoting vascular differentiation.By similarity

Keywords - PTMi

Hydroxylation

Proteomic databases

PaxDbiQ9Y574.
PeptideAtlasiQ9Y574.
PRIDEiQ9Y574.

PTM databases

iPTMnetiQ9Y574.
PhosphoSitePlusiQ9Y574.

Expressioni

Gene expression databases

BgeeiENSG00000005981.
CleanExiHS_ASB4.
GenevisibleiQ9Y574. HS.

Organism-specific databases

HPAiHPA055240.

Interactioni

Subunit structurei

Interacts with HIF1AN. Component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex formed of CUL2 or CUL5, Elongin BC (TCEB1 and TCEB2), RBX1 and ASB4.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi119669. 27 interactors.
IntActiQ9Y574. 1 interactor.
STRINGi9606.ENSP00000321388.

Structurei

3D structure databases

ProteinModelPortaliQ9Y574.
SMRiQ9Y574.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati74 – 103ANK 1Add BLAST30
Repeati106 – 135ANK 2Add BLAST30
Repeati139 – 168ANK 3Add BLAST30
Repeati174 – 203ANK 4Add BLAST30
Repeati207 – 247ANK 5Add BLAST41
Repeati251 – 280ANK 6Add BLAST30
Domaini371 – 423SOCS boxPROSITE-ProRule annotationAdd BLAST53

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni238 – 239Essential for interaction with HIF1ANBy similarity2
Regioni244 – 246Essential for interaction with HIF1ANBy similarity3

Domaini

The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin-protein ligase complexes.By similarity

Sequence similaritiesi

Belongs to the ankyrin SOCS box (ASB) family.Curated
Contains 6 ANK repeats.PROSITE-ProRule annotation
Contains 1 SOCS box domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00390000011960.
HOGENOMiHOG000232066.
HOVERGENiHBG024357.
InParanoidiQ9Y574.
KOiK10326.
OMAiHHLICRM.
OrthoDBiEOG091G064L.
PhylomeDBiQ9Y574.
TreeFamiTF331836.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001496. SOCS_box.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF07525. SOCS_box. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 6 hits.
SM00969. SOCS_box. 1 hit.
[Graphical view]
SUPFAMiSSF158235. SSF158235. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS50225. SOCS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y574-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGTTAPVTK SGAAKLVKRN FLEALKSNDF GKLKAILIQR QIDVDTVFEV
60 70 80 90 100
EDENMVLASY KQGYWLPSYK LKSSWATGLH LSVLFGHVEC LLVLLDHNAT
110 120 130 140 150
INCRPNGKTP LHVACEMANV DCVKILCDRG AKLNCYSLSG HTALHFCTTP
160 170 180 190 200
SSILCAKQLV WRGANVNMKT NNQDEETPLH TAAHFGLSEL VAFYVEHGAI
210 220 230 240 250
VDSVNAHMET PLAIAAYWAL RFKEQEYSTE HHLVCRMLLD YKAEVNARDD
260 270 280 290 300
DFKSPLHKAA WNCDHVLMHM MLEAGAEANL MDINGCAAIQ YVLKVTSVRP
310 320 330 340 350
AAQPEICYQL LLNHGAARIY PPQFHKVIQA CHSCPKAIEV VVNAYEHIRW
360 370 380 390 400
NTKWRRAIPD DDLEKYWDFY HSLFTVCCNS PRTLMHLSRC AIRRTLHNRC
410 420
HRAIPLLSLP LSLKKYLLLE PEGIIY
Length:426
Mass (Da):48,195
Last modified:November 1, 1999 - v1
Checksum:i4027F109A6C264A5
GO
Isoform 2 (identifier: Q9Y574-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     328-426: IQACHSCPKA...LLLEPEGIIY → RLCPVVSRLRKIQMAALSEISK

Note: No experimental confirmation available.
Show »
Length:349
Mass (Da):38,931
Checksum:i53801D22056907FD
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03351217V → L.Corresponds to variant rs35047380dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042668328 – 426IQACH…EGIIY → RLCPVVSRLRKIQMAALSEI SK in isoform 2. 1 PublicationAdd BLAST99

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156779 mRNA. Translation: AAD41896.1.
AC002451 Genomic DNA. No translation available.
AC003079 Genomic DNA. Translation: AAB83943.2.
CH471091 Genomic DNA. Translation: EAW76761.1.
CH471091 Genomic DNA. Translation: EAW76762.1.
CH236949 Genomic DNA. Translation: EAL24129.1.
CH236949 Genomic DNA. Translation: EAL24130.1.
BC113479 mRNA. Translation: AAI13480.1.
BC113481 mRNA. Translation: AAI13482.1.
BC025024 mRNA. Translation: AAH25024.1.
CCDSiCCDS5641.1. [Q9Y574-1]
CCDS5642.1. [Q9Y574-2]
RefSeqiNP_057200.1. NM_016116.2. [Q9Y574-1]
NP_665879.1. NM_145872.2. [Q9Y574-2]
UniGeneiHs.602765.
Hs.737518.

Genome annotation databases

EnsembliENST00000325885; ENSP00000321388; ENSG00000005981. [Q9Y574-1]
ENST00000428113; ENSP00000397070; ENSG00000005981. [Q9Y574-2]
GeneIDi51666.
KEGGihsa:51666.
UCSCiuc003unx.3. human. [Q9Y574-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156779 mRNA. Translation: AAD41896.1.
AC002451 Genomic DNA. No translation available.
AC003079 Genomic DNA. Translation: AAB83943.2.
CH471091 Genomic DNA. Translation: EAW76761.1.
CH471091 Genomic DNA. Translation: EAW76762.1.
CH236949 Genomic DNA. Translation: EAL24129.1.
CH236949 Genomic DNA. Translation: EAL24130.1.
BC113479 mRNA. Translation: AAI13480.1.
BC113481 mRNA. Translation: AAI13482.1.
BC025024 mRNA. Translation: AAH25024.1.
CCDSiCCDS5641.1. [Q9Y574-1]
CCDS5642.1. [Q9Y574-2]
RefSeqiNP_057200.1. NM_016116.2. [Q9Y574-1]
NP_665879.1. NM_145872.2. [Q9Y574-2]
UniGeneiHs.602765.
Hs.737518.

3D structure databases

ProteinModelPortaliQ9Y574.
SMRiQ9Y574.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119669. 27 interactors.
IntActiQ9Y574. 1 interactor.
STRINGi9606.ENSP00000321388.

PTM databases

iPTMnetiQ9Y574.
PhosphoSitePlusiQ9Y574.

Polymorphism and mutation databases

BioMutaiASB4.
DMDMi20532003.

Proteomic databases

PaxDbiQ9Y574.
PeptideAtlasiQ9Y574.
PRIDEiQ9Y574.

Protocols and materials databases

DNASUi51666.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000325885; ENSP00000321388; ENSG00000005981. [Q9Y574-1]
ENST00000428113; ENSP00000397070; ENSG00000005981. [Q9Y574-2]
GeneIDi51666.
KEGGihsa:51666.
UCSCiuc003unx.3. human. [Q9Y574-1]

Organism-specific databases

CTDi51666.
DisGeNETi51666.
GeneCardsiASB4.
HGNCiHGNC:16009. ASB4.
HPAiHPA055240.
MIMi605761. gene.
neXtProtiNX_Q9Y574.
OpenTargetsiENSG00000005981.
PharmGKBiPA25032.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00390000011960.
HOGENOMiHOG000232066.
HOVERGENiHBG024357.
InParanoidiQ9Y574.
KOiK10326.
OMAiHHLICRM.
OrthoDBiEOG091G064L.
PhylomeDBiQ9Y574.
TreeFamiTF331836.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciZFISH:ENSG00000005981-MONOMER.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

GenomeRNAii51666.
PROiQ9Y574.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000005981.
CleanExiHS_ASB4.
GenevisibleiQ9Y574. HS.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001496. SOCS_box.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF07525. SOCS_box. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 6 hits.
SM00969. SOCS_box. 1 hit.
[Graphical view]
SUPFAMiSSF158235. SSF158235. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS50225. SOCS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASB4_HUMAN
AccessioniPrimary (citable) accession number: Q9Y574
Secondary accession number(s): A4D1H6
, O14586, Q14D68, Q8TBT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.