##gff-version 3 Q9Y572 UniProtKB Chain 1 518 . . . ID=PRO_0000086610;Note=Receptor-interacting serine/threonine-protein kinase 3 Q9Y572 UniProtKB Domain 21 287 . . . Note=Protein kinase;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00159 Q9Y572 UniProtKB Region 355 443 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite Q9Y572 UniProtKB Region 476 518 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite Q9Y572 UniProtKB Motif 450 466 . . . Note=RIP homotypic interaction motif (RHIM);Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:29681455;Dbxref=PMID:29681455 Q9Y572 UniProtKB Compositional bias 372 440 . . . Note=Polar residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite Q9Y572 UniProtKB Active site 142 142 . . . Note=Proton acceptor;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:22265413;Dbxref=PMID:22265413 Q9Y572 UniProtKB Binding site 27 35 . . . Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00159 Q9Y572 UniProtKB Binding site 50 50 . . . Ontology_term=ECO:0000305,ECO:0000305,ECO:0000305;evidence=ECO:0000305|PubMed:10339433,ECO:0000305|PubMed:10358032,ECO:0000305|PubMed:22265414;Dbxref=PMID:10339433,PMID:10358032,PMID:22265414 Q9Y572 UniProtKB Modified residue 2 2 . . . Note=Phosphoserine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q9QZL0 Q9Y572 UniProtKB Modified residue 164 164 . . . Note=Phosphoserine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q9QZL0 Q9Y572 UniProtKB Modified residue 182 182 . . . Note=Phosphothreonine;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:29883609;Dbxref=PMID:29883609 Q9Y572 UniProtKB Modified residue 199 199 . . . Note=Phosphoserine%3B by autocatalysis;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:11734559,ECO:0000269|PubMed:19524512;Dbxref=PMID:11734559,PMID:19524512 Q9Y572 UniProtKB Modified residue 227 227 . . . Note=Phosphoserine%3B by autocatalysis;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:22265413,ECO:0000269|PubMed:29883609;Dbxref=PMID:22265413,PMID:29883609 Q9Y572 UniProtKB Modified residue 252 252 . . . Note=Phosphothreonine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q9QZL0 Q9Y572 UniProtKB Modified residue 299 299 . . . Note=Phosphoserine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q9QZL0 Q9Y572 UniProtKB Modified residue 333 333 . . . Note=Phosphothreonine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q9QZL0 Q9Y572 UniProtKB Modified residue 389 389 . . . Note=Phosphoserine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q9QZL0 Q9Y572 UniProtKB Modified residue 401 401 . . . Note=Phosphothreonine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q9QZL0 Q9Y572 UniProtKB Cross-link 363 363 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin);Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:29883609;Dbxref=PMID:29883609 Q9Y572 UniProtKB Alternative sequence 220 518 . . . ID=VSP_035106;Note=In isoform 2. VELPTEPSLVYEAVCNRQNRPSLAELPQAGPETPGLEGLKELMQLCWSSEPKDRPSFQECLPKTDEVFQMVENNMNAAVSTVKDFLSQLRSSNRRFSIPESGQGGTEMDGFRRTIENQHSRNDVMVSEWLNKLNLEEPPSSVPKKCPSLTKRSRAQEEQVPQAWTAGTSSDSMAQPPQTPETSTFRNQMPSPTSTGTPSPGPRGNQGAERQGMNWSCRTPEPNPVTGRPLVNIYNCSGVQVGDNNYLTMQQTTALPTWGLAPSGKGRGLQHPPPVGSQEGPKDPEAWSRPQGWYNHSGK->CQPNHHSCTKQCATGRTGLHWLSCPKPGLRLPA;Ontology_term=ECO:0000303;evidence=ECO:0000303|PubMed:15896315;Dbxref=PMID:15896315 Q9Y572 UniProtKB Alternative sequence 222 518 . . . ID=VSP_035107;Note=In isoform 3. LPTEPSLVYEAVCNRQNRPSLAELPQAGPETPGLEGLKELMQLCWSSEPKDRPSFQECLPKTDEVFQMVENNMNAAVSTVKDFLSQLRSSNRRFSIPESGQGGTEMDGFRRTIENQHSRNDVMVSEWLNKLNLEEPPSSVPKKCPSLTKRSRAQEEQVPQAWTAGTSSDSMAQPPQTPETSTFRNQMPSPTSTGTPSPGPRGNQGAERQGMNWSCRTPEPNPVTGRPLVNIYNCSGVQVGDNNYLTMQQTTALPTWGLAPSGKGRGLQHPPPVGSQEGPKDPEAWSRPQGWYNHSGK->CKTLGGFWDP;Ontology_term=ECO:0000303,ECO:0000303;evidence=ECO:0000303|PubMed:14702039,ECO:0000303|PubMed:15896315;Dbxref=PMID:14702039,PMID:15896315 Q9Y572 UniProtKB Natural variant 260 260 . . . ID=VAR_051664;Note=E->V;Dbxref=dbSNP:rs7153640 Q9Y572 UniProtKB Natural variant 300 300 . . . ID=VAR_041048;Note=T->M;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17344846;Dbxref=dbSNP:rs34106261,PMID:17344846 Q9Y572 UniProtKB Natural variant 492 492 . . . ID=VAR_041049;Note=P->Q;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17344846;Dbxref=dbSNP:rs3212254,PMID:17344846 Q9Y572 UniProtKB Mutagenesis 50 50 . . . Note=Abolishes kinase activity. Loss of PGAM5- and MLKL-binding. No effect on RIPK1-binding. Loss of interaction with PELI1 and PELI1-mediated ubiquitination. No loss of STUB1-mediated ubiquitination. K->A;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:10339433,ECO:0000269|PubMed:10358032,ECO:0000269|PubMed:22265414,ECO:0000269|PubMed:29883609;Dbxref=PMID:10339433,PMID:10358032,PMID:22265414,PMID:29883609 Q9Y572 UniProtKB Mutagenesis 50 50 . . . Note=Abolishes kinase activity. K->D;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:10339433,ECO:0000269|PubMed:10358032,ECO:0000269|PubMed:22265414;Dbxref=PMID:10339433,PMID:10358032,PMID:22265414 Q9Y572 UniProtKB Mutagenesis 142 142 . . . Note=Abolishes kinase activity and ability to mediate necroptosis. D->N;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22265413;Dbxref=PMID:22265413 Q9Y572 UniProtKB Mutagenesis 182 182 . . . Note=Abolishes kinase activity. Loss of interaction with PELI1 and PELI1-mediated ubiquitination. No loss of interaction with STUB1 and STUB1-mediated ubiquitination. No loss of interaction with RIPK1. Loss of ability to mediate TNF-induced necroptosis. Loss of autophosphorylation at Ser-227. T->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:29883609;Dbxref=PMID:29883609 Q9Y572 UniProtKB Mutagenesis 182 182 . . . Note=No loss of interaction with PELI1 and PELI1-mediated ubiquitination. No loss of interaction with RIPK1 and MLKL. T->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:29883609;Dbxref=PMID:29883609 Q9Y572 UniProtKB Mutagenesis 185 185 . . . Note=Loss of interaction with PELI1 and PELI1-mediated ubiquitination. Y->A%2CF;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:29883609;Dbxref=PMID:29883609 Q9Y572 UniProtKB Mutagenesis 227 227 . . . Note=Abolishes ability to mediate necroptosis. Partial loss of kinase activity. No loss of PELI1-mediated degradation. S->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:22265413,ECO:0000269|PubMed:29883609;Dbxref=PMID:22265413,PMID:29883609 Q9Y572 UniProtKB Mutagenesis 227 227 . . . Note=No loss of PELI1-mediated degradation. S->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:29883609;Dbxref=PMID:29883609 Q9Y572 UniProtKB Mutagenesis 363 363 . . . Note=Loss of PELI1-mediated ubiquitination. No loss of interaction with PELI1. K->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:29883609;Dbxref=PMID:29883609 Q9Y572 UniProtKB Mutagenesis 458 461 . . . Note=Abolished cleavage by S.flexneri OspD3. VQVG->AAAA;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32657447;Dbxref=PMID:32657447 Q9Y572 UniProtKB Sequence conflict 30 30 . . . Note=G->D;Ontology_term=ECO:0000305;evidence=ECO:0000305 Q9Y572 UniProtKB Sequence conflict 150 150 . . . Note=L->P;Ontology_term=ECO:0000305;evidence=ECO:0000305 Q9Y572 UniProtKB Sequence conflict 309 309 . . . Note=R->K;Ontology_term=ECO:0000305;evidence=ECO:0000305 Q9Y572 UniProtKB Helix 18 20 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Beta strand 21 26 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Beta strand 35 40 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Turn 41 43 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Beta strand 45 51 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Turn 54 56 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MX3 Q9Y572 UniProtKB Helix 57 65 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Beta strand 75 80 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Beta strand 84 87 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MX3 Q9Y572 UniProtKB Beta strand 91 95 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Helix 102 107 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Helix 114 133 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Beta strand 134 136 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Helix 145 147 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Beta strand 148 150 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Beta strand 156 158 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Helix 161 163 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MX3 Q9Y572 UniProtKB Beta strand 165 169 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MX3 Q9Y572 UniProtKB Helix 182 185 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Helix 188 190 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Beta strand 193 195 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MX3 Q9Y572 UniProtKB Helix 200 216 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Helix 226 233 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Helix 242 244 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Beta strand 249 252 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MX3 Q9Y572 UniProtKB Helix 255 265 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Helix 270 272 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Helix 276 290 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Helix 291 293 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Helix 294 309 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MON Q9Y572 UniProtKB Beta strand 450 461 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:5V7Z Q9Y572 UniProtKB Beta strand 466 472 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7DAC