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Protein

Protein phosphatase methylesterase 1

Gene

PPME1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme.1 Publication

Catalytic activityi

[Phosphatase 2A protein]-leucine methyl ester + H2O = [phosphatase 2A protein]-leucine + methanol.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1561 Publication1
Active sitei1811 Publication1
Active sitei3491 Publication1

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • protein C-terminal methylesterase activity Source: HGNC
  • protein phosphatase 2A binding Source: HGNC
  • protein phosphatase binding Source: HGNC
  • protein phosphatase inhibitor activity Source: ProtInc
  • protein phosphatase type 2A regulator activity Source: HGNC

GO - Biological processi

  • protein demethylation Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16514.
ZFISH:MONOMER-16514.
BRENDAi3.1.1.89. 2681.

Protein family/group databases

ESTHERihuman-PPME1. PPase_methylesterase_euk.
MEROPSiS33.984.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase methylesterase 1 (EC:3.1.1.89)
Short name:
PME-1
Gene namesi
Name:PPME1
Synonyms:PME1
ORF Names:PP2593, PRO0750
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:30178. PPME1.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: BHF-UCL
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi51400.
OpenTargetsiENSG00000214517.
PharmGKBiPA142671152.

Chemistry databases

ChEMBLiCHEMBL1293320.
GuidetoPHARMACOLOGYi2875.

Polymorphism and mutation databases

DMDMi47606055.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000903902 – 386Protein phosphatase methylesterase 1Add BLAST385

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphoserineCombined sources1
Modified residuei16Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei16Omega-N-methylarginine; alternateCombined sources1
Modified residuei42PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by SIK1 following increases in intracellular sodium, leading to dissociation from the protein phosphatase 2A (PP2A) complex and subsequent dephosphorylation of sodium/potassium-transporting ATPase ATP1A1.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ9Y570.
PaxDbiQ9Y570.
PeptideAtlasiQ9Y570.
PRIDEiQ9Y570.

2D gel databases

REPRODUCTION-2DPAGEIPI00007694.

PTM databases

iPTMnetiQ9Y570.
PhosphoSitePlusiQ9Y570.
SwissPalmiQ9Y570.

Expressioni

Gene expression databases

BgeeiENSG00000214517.
CleanExiHS_PPME1.
ExpressionAtlasiQ9Y570. baseline and differential.
GenevisibleiQ9Y570. HS.

Organism-specific databases

HPAiCAB004541.
HPA043900.
HPA064396.

Interactioni

Subunit structurei

Binds PPP2CA and PPP2CB.1 Publication

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • protein phosphatase 2A binding Source: HGNC
  • protein phosphatase binding Source: HGNC

Protein-protein interaction databases

BioGridi119524. 96 interactors.
IntActiQ9Y570. 7 interactors.
STRINGi9606.ENSP00000329867.

Chemistry databases

BindingDBiQ9Y570.

Structurei

Secondary structure

1386
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi46 – 48Combined sources3
Beta strandi51 – 60Combined sources10
Beta strandi63 – 72Combined sources10
Beta strandi74 – 76Combined sources3
Beta strandi78 – 82Combined sources5
Helixi89 – 92Combined sources4
Helixi93 – 100Combined sources8
Beta strandi106 – 110Combined sources5
Helixi128 – 143Combined sources16
Beta strandi144 – 146Combined sources3
Beta strandi150 – 155Combined sources6
Helixi157 – 167Combined sources11
Beta strandi174 – 181Combined sources8
Helixi184 – 200Combined sources17
Beta strandi205 – 207Combined sources3
Helixi208 – 217Combined sources10
Helixi224 – 234Combined sources11
Beta strandi235 – 237Combined sources3
Beta strandi285 – 288Combined sources4
Helixi291 – 294Combined sources4
Helixi295 – 302Combined sources8
Helixi305 – 311Combined sources7
Beta strandi312 – 314Combined sources3
Beta strandi316 – 322Combined sources7
Helixi323 – 325Combined sources3
Helixi328 – 335Combined sources8
Beta strandi339 – 343Combined sources5
Helixi351 – 354Combined sources4
Helixi356 – 369Combined sources14
Beta strandi372 – 375Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3C5VX-ray2.00A39-386[»]
3C5WX-ray2.80P284-386[»]
P39-238[»]
ProteinModelPortaliQ9Y570.
SMRiQ9Y570.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y570.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi256 – 263Poly-Glu8

Sequence similaritiesi

Belongs to the AB hydrolase superfamily.Curated

Phylogenomic databases

eggNOGiKOG2564. Eukaryota.
COG0596. LUCA.
GeneTreeiENSGT00390000004396.
HOGENOMiHOG000116699.
HOVERGENiHBG053622.
InParanoidiQ9Y570.
KOiK13617.
OMAiRPWRWRT.
OrthoDBiEOG091G0D0P.
PhylomeDBiQ9Y570.
TreeFamiTF314697.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR016812. PPase_methylesterase_euk.
[Graphical view]
PANTHERiPTHR14189. PTHR14189. 1 hit.
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
PIRSFiPIRSF022950. PPase_methylesterase_euk. 1 hit.
SUPFAMiSSF53474. SSF53474. 2 hits.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y570-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSALEKSMHL GRLPSRPPLP GSGGSQSGAK MRMGPGRKRD FSPVPWSQYF
60 70 80 90 100
ESMEDVEVEN ETGKDTFRVY KSGSEGPVLL LLHGGGHSAL SWAVFTAAII
110 120 130 140 150
SRVQCRIVAL DLRSHGETKV KNPEDLSAET MAKDVGNVVE AMYGDLPPPI
160 170 180 190 200
MLIGHSMGGA IAVHTASSNL VPSLLGLCMI DVVEGTAMDA LNSMQNFLRG
210 220 230 240 250
RPKTFKSLEN AIEWSVKSGQ IRNLESARVS MVGQVKQCEG ITSPEGSKSI
260 270 280 290 300
VEGIIEEEEE DEEGSESISK RKKEDDMETK KDHPYTWRIE LAKTEKYWDG
310 320 330 340 350
WFRGLSNLFL SCPIPKLLLL AGVDRLDKDL TIGQMQGKFQ MQVLPQCGHA
360 370 380
VHEDAPDKVA EAVATFLIRH RFAEPIGGFQ CVFPGC
Length:386
Mass (Da):42,315
Last modified:January 23, 2007 - v3
Checksum:i37B25324583E8578
GO
Isoform 2 (identifier: Q9Y570-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-187: Missing.

Note: No experimental confirmation available.
Show »
Length:199
Mass (Da):22,462
Checksum:iF39B21A7AD5C5115
GO
Isoform 3 (identifier: Q9Y570-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-276: Missing.
     360-386: AEAVATFLIRHRFAEPIGGFQCVFPGC → SLVLSDCKRT...LCYFIPGPCG

Note: No experimental confirmation available.
Show »
Length:161
Mass (Da):18,214
Checksum:i53E51B3B7916DBFB
GO
Isoform 4 (identifier: Q9Y570-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     278-278: E → EGLPSETQNLLLFLQ

Note: Gene prediction based on EST data.
Show »
Length:400
Mass (Da):43,870
Checksum:i0F9F02DDFABA6C3B
GO

Sequence cautioni

The sequence AAG22477 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti309F → S in BAA91661 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0103351 – 276Missing in isoform 3. 1 PublicationAdd BLAST276
Alternative sequenceiVSP_0103361 – 187Missing in isoform 2. 1 PublicationAdd BLAST187
Alternative sequenceiVSP_054818278E → EGLPSETQNLLLFLQ in isoform 4. Curated1
Alternative sequenceiVSP_010337360 – 386AEAVA…VFPGC → SLVLSDCKRTTVRITLDVTE DKSLSLSLHCLQQLLWSLCR CSSTSSPTSPWQLLMVLVLC ICAEELLTLCYFIPGPCG in isoform 3. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF157028 mRNA. Translation: AAD44976.1.
AF111853 mRNA. Translation: AAF16692.1.
AK001381 mRNA. Translation: BAA91661.1.
AK022725 mRNA. Translation: BAG51108.1.
AK123288 mRNA. Translation: BAC85574.1.
AP000577 Genomic DNA. No translation available.
AP002392 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW74932.1.
BC003046 mRNA. Translation: AAH03046.1.
BC050705 mRNA. Translation: AAH50705.1.
AF193049 mRNA. Translation: AAG22477.1. Sequence problems.
CCDSiCCDS44678.1. [Q9Y570-1]
CCDS60891.1. [Q9Y570-4]
RefSeqiNP_001258522.1. NM_001271593.1. [Q9Y570-4]
NP_057231.1. NM_016147.2. [Q9Y570-1]
UniGeneiHs.503251.

Genome annotation databases

EnsembliENST00000328257; ENSP00000329867; ENSG00000214517. [Q9Y570-1]
ENST00000398427; ENSP00000381461; ENSG00000214517. [Q9Y570-4]
GeneIDi51400.
KEGGihsa:51400.
UCSCiuc001ouw.5. human. [Q9Y570-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF157028 mRNA. Translation: AAD44976.1.
AF111853 mRNA. Translation: AAF16692.1.
AK001381 mRNA. Translation: BAA91661.1.
AK022725 mRNA. Translation: BAG51108.1.
AK123288 mRNA. Translation: BAC85574.1.
AP000577 Genomic DNA. No translation available.
AP002392 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW74932.1.
BC003046 mRNA. Translation: AAH03046.1.
BC050705 mRNA. Translation: AAH50705.1.
AF193049 mRNA. Translation: AAG22477.1. Sequence problems.
CCDSiCCDS44678.1. [Q9Y570-1]
CCDS60891.1. [Q9Y570-4]
RefSeqiNP_001258522.1. NM_001271593.1. [Q9Y570-4]
NP_057231.1. NM_016147.2. [Q9Y570-1]
UniGeneiHs.503251.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3C5VX-ray2.00A39-386[»]
3C5WX-ray2.80P284-386[»]
P39-238[»]
ProteinModelPortaliQ9Y570.
SMRiQ9Y570.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119524. 96 interactors.
IntActiQ9Y570. 7 interactors.
STRINGi9606.ENSP00000329867.

Chemistry databases

BindingDBiQ9Y570.
ChEMBLiCHEMBL1293320.
GuidetoPHARMACOLOGYi2875.

Protein family/group databases

ESTHERihuman-PPME1. PPase_methylesterase_euk.
MEROPSiS33.984.

PTM databases

iPTMnetiQ9Y570.
PhosphoSitePlusiQ9Y570.
SwissPalmiQ9Y570.

Polymorphism and mutation databases

DMDMi47606055.

2D gel databases

REPRODUCTION-2DPAGEIPI00007694.

Proteomic databases

EPDiQ9Y570.
PaxDbiQ9Y570.
PeptideAtlasiQ9Y570.
PRIDEiQ9Y570.

Protocols and materials databases

DNASUi51400.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000328257; ENSP00000329867; ENSG00000214517. [Q9Y570-1]
ENST00000398427; ENSP00000381461; ENSG00000214517. [Q9Y570-4]
GeneIDi51400.
KEGGihsa:51400.
UCSCiuc001ouw.5. human. [Q9Y570-1]

Organism-specific databases

CTDi51400.
DisGeNETi51400.
GeneCardsiPPME1.
HGNCiHGNC:30178. PPME1.
HPAiCAB004541.
HPA043900.
HPA064396.
MIMi611117. gene.
neXtProtiNX_Q9Y570.
OpenTargetsiENSG00000214517.
PharmGKBiPA142671152.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2564. Eukaryota.
COG0596. LUCA.
GeneTreeiENSGT00390000004396.
HOGENOMiHOG000116699.
HOVERGENiHBG053622.
InParanoidiQ9Y570.
KOiK13617.
OMAiRPWRWRT.
OrthoDBiEOG091G0D0P.
PhylomeDBiQ9Y570.
TreeFamiTF314697.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16514.
ZFISH:MONOMER-16514.
BRENDAi3.1.1.89. 2681.

Miscellaneous databases

ChiTaRSiPPME1. human.
EvolutionaryTraceiQ9Y570.
GenomeRNAii51400.
PROiQ9Y570.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000214517.
CleanExiHS_PPME1.
ExpressionAtlasiQ9Y570. baseline and differential.
GenevisibleiQ9Y570. HS.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR016812. PPase_methylesterase_euk.
[Graphical view]
PANTHERiPTHR14189. PTHR14189. 1 hit.
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
PIRSFiPIRSF022950. PPase_methylesterase_euk. 1 hit.
SUPFAMiSSF53474. SSF53474. 2 hits.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPME1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y570
Secondary accession number(s): B3KMU6
, B5MEE7, J3QT22, Q8WYG8, Q9NVT5, Q9UI18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 145 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.