Q9Y561 (LRP12_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Low-density lipoprotein receptor-related protein 12
Short name=LRP-12

Alternative name(s):
Suppressor of tumorigenicity 7 protein

Gene names

Name:	LRP12
Synonyms:	ST7

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	859 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable receptor, which may be involved in the internalization of lipophilic molecules and/or signal transduction. May act as a tumor suppressor. Ref.6
Subunit structure	May interact with GNB2L1, ZFYVE9 and NMRK2.
Subcellular location	Membrane; Single-pass type I membrane protein. Membrane › coated pit Ref.6.
Tissue specificity	Widely expressed in heart, skeletal muscle, brain, lung, placenta and pancreas, but not in tissues consisting of a large number of epithelial cells, such as liver and kidney. Expressed at very low levels in a number of tumor-derived cell lines. Ref.1
Sequence similarities	Belongs to the LDLR family. Contains 2 CUB domains. Contains 5 LDL-receptor class A domains.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Coated pit Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of growth Non-traceable author statement Ref.1. Source: UniProtKB signal transduction Non-traceable author statement Ref.6. Source: UniProtKB
Cellular_component	coated pit Inferred from electronic annotation. Source: UniProtKB-SubCell integral to plasma membrane Inferred from direct assay Ref.6. Source: UniProtKB
Molecular_function	low-density lipoprotein receptor activity Non-traceable author statement Ref.6. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GNB2L1	P63244	2	EBI-296693,EBI-296739
NMRK2	Q9NPI5	2	EBI-296693,EBI-514059
ZFYVE9	O95405	2	EBI-296693,EBI-296817

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y561-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y561-2)

The sequence of this isoform differs from the canonical sequence as follows:
     27-45: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Potential
	Chain	33 – 859	827	Low-density lipoprotein receptor-related protein 12		PRO_0000017339
Regions
	Topological domain	33 – 492	460	Extracellular Potential
	Transmembrane	493 – 513	21	Helical; Potential
	Topological domain	514 – 859	346	Cytoplasmic Potential
	Domain	47 – 159	113	CUB 1
	Domain	165 – 201	37	LDL-receptor class A 1
	Domain	214 – 255	42	LDL-receptor class A 2
	Domain	259 – 372	114	CUB 2
	Domain	374 – 411	38	LDL-receptor class A 3
	Domain	412 – 449	38	LDL-receptor class A 4
	Domain	450 – 486	37	LDL-receptor class A 5
Amino acid modifications
	Glycosylation	75	1	N-linked (GlcNAc...) Potential
	Glycosylation	146	1	N-linked (GlcNAc...) Potential
	Glycosylation	284	1	N-linked (GlcNAc...) Potential
	Glycosylation	366	1	N-linked (GlcNAc...) Potential
	Glycosylation	409	1	N-linked (GlcNAc...) Potential
	Glycosylation	441	1	N-linked (GlcNAc...) Potential
	Disulfide bond	47 ↔ 76		By similarity
	Disulfide bond	103 ↔ 122		By similarity
	Disulfide bond	166 ↔ 178		By similarity
	Disulfide bond	173 ↔ 191		By similarity
	Disulfide bond	185 ↔ 200		By similarity
	Disulfide bond	215 ↔ 232		By similarity
	Disulfide bond	222 ↔ 245		By similarity
	Disulfide bond	239 ↔ 254		By similarity
	Disulfide bond	259 ↔ 285		By similarity
	Disulfide bond	375 ↔ 388		By similarity
	Disulfide bond	382 ↔ 401		By similarity
	Disulfide bond	395 ↔ 410		By similarity
	Disulfide bond	413 ↔ 426		By similarity
	Disulfide bond	420 ↔ 439		By similarity
	Disulfide bond	433 ↔ 448		By similarity
	Disulfide bond	451 ↔ 463		By similarity
	Disulfide bond	458 ↔ 476		By similarity
	Disulfide bond	470 ↔ 485		By similarity
Natural variations
	Alternative sequence	27 – 45	19	Missing in isoform 2.		VSP_040992
	Natural variant	694	1	S → G. Corresponds to variant rs16871494 [ dbSNP | Ensembl ].		VAR_049766

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 41A8FD8121E32A92	FASTA	859	94,984
	10 20 30 40 50 60 MACRWSTKES PRWRSALLLL FLAGVYGNGA LAEHSENVHI SGVSTACGET PEQIRAPSGI 70 80 90 100 110 120 ITSPGWPSEY PAKINCSWFI RANPGEIITI SFQDFDIQGS RRCNLDWLTI ETYKNIESYR 130 140 150 160 170 180 ACGSTIPPPY ISSQDHIWIR FHSDDNISRK GFRLAYFSGK SEEPNCACDQ FRCGNGKCIP 190 200 210 220 230 240 EAWKCNNMDE CGDSSDEEIC AKEANPPTAA AFQPCAYNQF QCLSRFTKVY TCLPESLKCD 250 260 270 280 290 300 GNIDCLDLGD EIDCDVPTCG QWLKYFYGTF NSPNYPDFYP PGSNCTWLID TGDHRKVILR 310 320 330 340 350 360 FTDFKLDGTG YGDYVKIYDG LEENPHKLLR VLTAFDSHAP LTVVSSSGQI RVHFCADKVN 370 380 390 400 410 420 AARGFNATYQ VDGFCLPWEI PCGGNWGCYT EQQRCDGYWH CPNGRDETNC TMCQKEEFPC 430 440 450 460 470 480 SRNGVCYPRS DRCNYQNHCP NGSDEKNCFF CQPGNFHCKN NRCVFESWVC DSQDDCGDGS 490 500 510 520 530 540 DEENCPVIVP TRVITAAVIG SLICGLLLVI ALGCTCKLYS LRMFERRSFE TQLSRVEAEL 550 560 570 580 590 600 LRREAPPSYG QLIAQGLIPP VEDFPVCSPN QASVLENLRL AVRSQLGFTS VRLPMAGRSS 610 620 630 640 650 660 NIWNRIFNFA RSRHSGSLAL VSADGDEVVP SQSTSREPER NHTHRSLFSV ESDDTDTENE 670 680 690 700 710 720 RRDMAGASGG VAAPLPQKVP PTTAVEATVG ACASSSTQST RGGHADNGRD VTSVEPPSVS 730 740 750 760 770 780 PARHQLTSAL SRMTQGLRWV RFTLGRSSSL SQNQSPLRQL DNGVSGREDD DDVEMLIPIS 790 800 810 820 830 840 DGSSDFDVND CSRPLLDLAS DQGQGLRQPY NATNPGVRPS NRDGPCERCG IVHTAQIPDT 850 CLEVTLKNET SDDEALLLC
	Isoform 2 [UniParc]. Checksum: 6C6C3B315296D717	FASTA	840	93,123
	10 20 30 40 50 60 MACRWSTKES PRWRSALLLL FLAGVYACGE TPEQIRAPSG IITSPGWPSE YPAKINCSWF 70 80 90 100 110 120 IRANPGEIIT ISFQDFDIQG SRRCNLDWLT IETYKNIESY RACGSTIPPP YISSQDHIWI 130 140 150 160 170 180 RFHSDDNISR KGFRLAYFSG KSEEPNCACD QFRCGNGKCI PEAWKCNNMD ECGDSSDEEI 190 200 210 220 230 240 CAKEANPPTA AAFQPCAYNQ FQCLSRFTKV YTCLPESLKC DGNIDCLDLG DEIDCDVPTC 250 260 270 280 290 300 GQWLKYFYGT FNSPNYPDFY PPGSNCTWLI DTGDHRKVIL RFTDFKLDGT GYGDYVKIYD 310 320 330 340 350 360 GLEENPHKLL RVLTAFDSHA PLTVVSSSGQ IRVHFCADKV NAARGFNATY QVDGFCLPWE 370 380 390 400 410 420 IPCGGNWGCY TEQQRCDGYW HCPNGRDETN CTMCQKEEFP CSRNGVCYPR SDRCNYQNHC 430 440 450 460 470 480 PNGSDEKNCF FCQPGNFHCK NNRCVFESWV CDSQDDCGDG SDEENCPVIV PTRVITAAVI 490 500 510 520 530 540 GSLICGLLLV IALGCTCKLY SLRMFERRSF ETQLSRVEAE LLRREAPPSY GQLIAQGLIP 550 560 570 580 590 600 PVEDFPVCSP NQASVLENLR LAVRSQLGFT SVRLPMAGRS SNIWNRIFNF ARSRHSGSLA 610 620 630 640 650 660 LVSADGDEVV PSQSTSREPE RNHTHRSLFS VESDDTDTEN ERRDMAGASG GVAAPLPQKV 670 680 690 700 710 720 PPTTAVEATV GACASSSTQS TRGGHADNGR DVTSVEPPSV SPARHQLTSA LSRMTQGLRW 730 740 750 760 770 780 VRFTLGRSSS LSQNQSPLRQ LDNGVSGRED DDDVEMLIPI SDGSSDFDVN DCSRPLLDLA 790 800 810 820 830 840 SDQGQGLRQP YNATNPGVRP SNRDGPCERC GIVHTAQIPD TCLEVTLKNE TSDDEALLLC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a novel gene encoding a putative transmembrane protein with altered expression in some human transformed and tumor-derived cell lines." Qing J., Wei D., Maher V.M., McCormick J.J. Oncogene 18:335-342(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain.
[3]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Retinal pigment epithelium.
[6]	"ST7 is a novel low-density lipoprotein receptor-related protein (LRP) with a cytoplasmic tail that interacts with proteins related to signal transduction pathways." Battle M.A., Maher V.M., McCormick J.J. Biochemistry 42:7270-7282(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, POSSIBLE INTERACTION WITH GNB2L1; ZFYVE9 AND NMRK2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF166350 mRNA. Translation: AAD44360.1. AK289752 mRNA. Translation: BAF82441.1. AK299374 mRNA. Translation: BAG61364.1. AC087370 Genomic DNA. No translation available. AC090827 Genomic DNA. No translation available. AP002847 Genomic DNA. No translation available. CH471060 Genomic DNA. Translation: EAW91897.1. BC032109 mRNA. Translation: AAH32109.1.
IPI	IPI00021027. IPI00914038.
RefSeq	NP_001129175.1. NM_001135703.2. NP_038465.1. NM_013437.4.
UniGene	Hs.600630. Hs.715867.
3D structure databases
ProteinModelPortal	Q9Y561.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y561. 6 interactions.
STRING	9606.ENSP00000276654.
PTM databases
PhosphoSite	Q9Y561.
Polymorphism databases
DMDM	25091287.
Proteomic databases
PaxDb	Q9Y561.
PRIDE	Q9Y561.
Protocols and materials databases
DNASU	29967.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000276654; ENSP00000276654; ENSG00000147650. ENST00000424843; ENSP00000399148; ENSG00000147650.
GeneID	29967.
KEGG	hsa:29967.
UCSC	uc003yma.3. human. uc003ymb.3. human.
Organism-specific databases
CTD	29967.
GeneCards	GC08M105501.
HGNC	HGNC:31708. LRP12.
HPA	HPA017245.
neXtProt	NX_Q9Y561.
PharmGKB	PA134921850.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG242928.
HOGENOM	HOG000013017.
HOVERGEN	HBG103190.
InParanoid	Q9Y561.
OMA	PTCGQWL.
PhylomeDB	Q9Y561.
Gene expression databases
ArrayExpress	Q9Y561.
Bgee	Q9Y561.
CleanEx	HS_LRP12. HS_ST7.
Genevestigator	Q9Y561.
GermOnline	ENSG00000147650. Homo sapiens.
Family and domain databases
Gene3D	2.60.120.290. 2 hits. 4.10.400.10. 5 hits.
InterPro	IPR000859. CUB_dom. IPR023415. LDLR_class-A_CS. IPR002172. LDrepeatLR_classA_rpt. [Graphical view]
Pfam	PF00431. CUB. 2 hits. PF00057. Ldl_recept_a. 3 hits. [Graphical view]
SMART	SM00042. CUB. 2 hits. SM00192. LDLa. 5 hits. [Graphical view]
SUPFAM	SSF49854. CUB. 2 hits. SSF57424. LDL_rcpt_classA_cys-rich. 5 hits.
PROSITE	PS01180. CUB. 2 hits. PS01209. LDLRA_1. 2 hits. PS50068. LDLRA_2. 5 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	29967.
NextBio	52701.

Entry information

Entry name

LRP12_HUMAN

Accession

Primary (citable) accession number: Q9Y561
Secondary accession number(s): A8K137, B4DRQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.