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Protein

DNA-directed RNA polymerase III subunit RPC8

Gene

POLR3H

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).By similarity

GO - Molecular functioni

  • DNA binding Source: InterPro
  • DNA-directed RNA polymerase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity, Transcription

Enzyme and pathway databases

ReactomeiREACT_1036. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
REACT_118823. Cytosolic sensors of pathogen-associated DNA.
REACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
REACT_347. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
REACT_571. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
REACT_63. RNA Polymerase III Transcription Termination.
REACT_756. RNA Polymerase III Chain Elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC8
Short name:
RNA polymerase III subunit C8
Alternative name(s):
DNA-directed RNA polymerase III subunit H
RNA polymerase III subunit 22.9 kDa subunit
Short name:
RPC22.9
Gene namesi
Name:POLR3H
Synonyms:KIAA1665, RPC8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:30349. POLR3H.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: HPA
  • cytosol Source: Reactome
  • DNA-directed RNA polymerase III complex Source: UniProtKB
  • intracellular membrane-bounded organelle Source: HPA
  • nucleoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134994174.

Polymorphism and mutation databases

DMDMi29428071.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 204204DNA-directed RNA polymerase III subunit RPC8PRO_0000073994Add
BLAST

Proteomic databases

MaxQBiQ9Y535.
PaxDbiQ9Y535.
PRIDEiQ9Y535.

Expressioni

Gene expression databases

BgeeiQ9Y535.
CleanExiHS_POLR3H.
ExpressionAtlasiQ9Y535. baseline and differential.
GenevisibleiQ9Y535. HS.

Organism-specific databases

HPAiHPA046787.

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). Interacts with CRCP/RPC9. POLR3H/RPC8 and CRCP/RPC9 probably form a Pol III subcomplex.By similarity1 Publication

Protein-protein interaction databases

BioGridi128147. 18 interactions.
IntActiQ9Y535. 1 interaction.
STRINGi9606.ENSP00000347345.

Structurei

3D structure databases

ProteinModelPortaliQ9Y535.
SMRiQ9Y535. Positions 1-201.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1095.
GeneTreeiENSGT00390000004383.
HOGENOMiHOG000157370.
HOVERGENiHBG044510.
InParanoidiQ9Y535.
KOiK03022.
OMAiNVGLCIA.
OrthoDBiEOG70CR7V.
PhylomeDBiQ9Y535.
TreeFamiTF103053.

Family and domain databases

Gene3Di3.30.1490.120. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR013238. RNA_pol_III_Rbc25.
IPR005576. RNA_pol_Rpb7_N.
IPR004519. RNAP_E/RPC8.
[Graphical view]
PfamiPF08292. RNA_pol_Rbc25. 1 hit.
PF03876. SHS2_Rpb7-N. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF88798. SSF88798. 1 hit.
TIGRFAMsiTIGR00448. rpoE. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y535-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFVLVEMVDT VRIPPWQFER KLNDSIAEEL NKKLANKVVY NVGLCICLFD
60 70 80 90 100
ITKLEDAYVF PGDGASHTKV HFRCVVFHPF LDEILIGKIK GCSPEGVHVS
110 120 130 140 150
LGFFDDILIP PESLQQPAKF DEAEQVWVWE YETEEGAHDL YMDTGEEIRF
160 170 180 190 200
RVVDESFVDT SPTGPSSADA TTSSEELPKK EAPYTLVGSI SEPGLGLLSW

WTSN
Length:204
Mass (Da):22,918
Last modified:November 1, 1999 - v1
Checksum:iDE85D44A5770C5D9
GO
Isoform 2 (identifier: Q9Y535-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     70-98: Missing.

Show »
Length:175
Mass (Da):19,658
Checksum:i2213B9D553C5DEE9
GO

Sequence cautioni

The sequence BAB33335.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei70 – 9829Missing in isoform 2. 1 PublicationVSP_007067Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY092087 mRNA. Translation: AAM18217.1.
AB051452 mRNA. Translation: BAB33335.1. Different initiation.
CR456459 mRNA. Translation: CAG30345.1.
AL023553 Genomic DNA. Translation: CAB46023.1.
AL023553 Genomic DNA. Translation: CAQ10207.1.
CH471095 Genomic DNA. Translation: EAW60438.1.
BC017248 mRNA. Translation: AAH17248.1.
BC088367 mRNA. Translation: AAH88367.1.
CCDSiCCDS14018.1. [Q9Y535-1]
CCDS33651.1. [Q9Y535-2]
RefSeqiNP_001018060.1. NM_001018050.3. [Q9Y535-1]
NP_001018062.1. NM_001018052.3. [Q9Y535-2]
NP_001269813.1. NM_001282884.1. [Q9Y535-1]
NP_001269814.1. NM_001282885.1. [Q9Y535-1]
NP_612211.1. NM_138338.4. [Q9Y535-1]
UniGeneiHs.712617.

Genome annotation databases

EnsembliENST00000337566; ENSP00000337627; ENSG00000100413. [Q9Y535-2]
ENST00000355209; ENSP00000347345; ENSG00000100413.
ENST00000396504; ENSP00000379761; ENSG00000100413.
ENST00000407461; ENSP00000385315; ENSG00000100413.
GeneIDi171568.
KEGGihsa:171568.
UCSCiuc003baf.3. human. [Q9Y535-1]
uc003bai.2. human. [Q9Y535-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY092087 mRNA. Translation: AAM18217.1.
AB051452 mRNA. Translation: BAB33335.1. Different initiation.
CR456459 mRNA. Translation: CAG30345.1.
AL023553 Genomic DNA. Translation: CAB46023.1.
AL023553 Genomic DNA. Translation: CAQ10207.1.
CH471095 Genomic DNA. Translation: EAW60438.1.
BC017248 mRNA. Translation: AAH17248.1.
BC088367 mRNA. Translation: AAH88367.1.
CCDSiCCDS14018.1. [Q9Y535-1]
CCDS33651.1. [Q9Y535-2]
RefSeqiNP_001018060.1. NM_001018050.3. [Q9Y535-1]
NP_001018062.1. NM_001018052.3. [Q9Y535-2]
NP_001269813.1. NM_001282884.1. [Q9Y535-1]
NP_001269814.1. NM_001282885.1. [Q9Y535-1]
NP_612211.1. NM_138338.4. [Q9Y535-1]
UniGeneiHs.712617.

3D structure databases

ProteinModelPortaliQ9Y535.
SMRiQ9Y535. Positions 1-201.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128147. 18 interactions.
IntActiQ9Y535. 1 interaction.
STRINGi9606.ENSP00000347345.

Polymorphism and mutation databases

DMDMi29428071.

Proteomic databases

MaxQBiQ9Y535.
PaxDbiQ9Y535.
PRIDEiQ9Y535.

Protocols and materials databases

DNASUi171568.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000337566; ENSP00000337627; ENSG00000100413. [Q9Y535-2]
ENST00000355209; ENSP00000347345; ENSG00000100413.
ENST00000396504; ENSP00000379761; ENSG00000100413.
ENST00000407461; ENSP00000385315; ENSG00000100413.
GeneIDi171568.
KEGGihsa:171568.
UCSCiuc003baf.3. human. [Q9Y535-1]
uc003bai.2. human. [Q9Y535-2]

Organism-specific databases

CTDi171568.
GeneCardsiGC22M041921.
HGNCiHGNC:30349. POLR3H.
HPAiHPA046787.
neXtProtiNX_Q9Y535.
PharmGKBiPA134994174.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1095.
GeneTreeiENSGT00390000004383.
HOGENOMiHOG000157370.
HOVERGENiHBG044510.
InParanoidiQ9Y535.
KOiK03022.
OMAiNVGLCIA.
OrthoDBiEOG70CR7V.
PhylomeDBiQ9Y535.
TreeFamiTF103053.

Enzyme and pathway databases

ReactomeiREACT_1036. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
REACT_118823. Cytosolic sensors of pathogen-associated DNA.
REACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
REACT_347. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
REACT_571. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
REACT_63. RNA Polymerase III Transcription Termination.
REACT_756. RNA Polymerase III Chain Elongation.

Miscellaneous databases

GenomeRNAii171568.
NextBioi89296.
PROiQ9Y535.

Gene expression databases

BgeeiQ9Y535.
CleanExiHS_POLR3H.
ExpressionAtlasiQ9Y535. baseline and differential.
GenevisibleiQ9Y535. HS.

Family and domain databases

Gene3Di3.30.1490.120. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR013238. RNA_pol_III_Rbc25.
IPR005576. RNA_pol_Rpb7_N.
IPR004519. RNAP_E/RPC8.
[Graphical view]
PfamiPF08292. RNA_pol_Rbc25. 1 hit.
PF03876. SHS2_Rpb7-N. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF88798. SSF88798. 1 hit.
TIGRFAMsiTIGR00448. rpoE. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits."
    Hu P., Wu S., Sun Y., Yuan C.-C., Kobayashi R., Myers M.P., Hernandez N.
    Mol. Cell. Biol. 22:8044-8055(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1), IDENTIFICATION IN THE RNA POL III COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH RPC9.
  2. "Identification of novel transcribed sequences on human chromosome 22 by expressed sequence tag mapping."
    Hirosawa M., Nagase T., Murahashi Y., Kikuno R., Ohara O.
    DNA Res. 8:1-9(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  4. "The DNA sequence of human chromosome 22."
    Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M.
    , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H.
    Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain and Pituitary.
  7. "RNA polymerase III detects cytosolic DNA and induces type I interferons through the RIG-I pathway."
    Chiu Y.-H., Macmillan J.B., Chen Z.J.
    Cell 138:576-591(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate."
    Ablasser A., Bauernfeind F., Hartmann G., Latz E., Fitzgerald K.A., Hornung V.
    Nat. Immunol. 10:1065-1072(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiRPC8_HUMAN
AccessioniPrimary (citable) accession number: Q9Y535
Secondary accession number(s): B0QYH9
, Q5M7Y8, Q96AE3, Q9BY95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: November 1, 1999
Last modified: July 22, 2015
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.