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Q9Y535

- RPC8_HUMAN

UniProt

Q9Y535 - RPC8_HUMAN

Protein

DNA-directed RNA polymerase III subunit RPC8

Gene

POLR3H

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway By similarity.By similarity

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. DNA-directed RNA polymerase activity Source: UniProtKB

    GO - Biological processi

    1. defense response to virus Source: UniProtKB-KW
    2. gene expression Source: Reactome
    3. innate immune response Source: Reactome
    4. nucleobase-containing compound metabolic process Source: UniProtKB
    5. positive regulation of type I interferon production Source: Reactome
    6. termination of RNA polymerase III transcription Source: Reactome
    7. transcription elongation from RNA polymerase III promoter Source: Reactome
    8. transcription from RNA polymerase III promoter Source: UniProtKB
    9. transcription initiation from RNA polymerase III promoter Source: RefGenome

    Keywords - Biological processi

    Antiviral defense, Immunity, Innate immunity, Transcription

    Enzyme and pathway databases

    ReactomeiREACT_1036. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
    REACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
    REACT_347. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
    REACT_571. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
    REACT_63. RNA Polymerase III Transcription Termination.
    REACT_756. RNA Polymerase III Chain Elongation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-directed RNA polymerase III subunit RPC8
    Short name:
    RNA polymerase III subunit C8
    Alternative name(s):
    DNA-directed RNA polymerase III subunit H
    RNA polymerase III subunit 22.9 kDa subunit
    Short name:
    RPC22.9
    Gene namesi
    Name:POLR3H
    Synonyms:KIAA1665, RPC8
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 22

    Organism-specific databases

    HGNCiHGNC:30349. POLR3H.

    Subcellular locationi

    GO - Cellular componenti

    1. centrosome Source: HPA
    2. cytosol Source: Reactome
    3. DNA-directed RNA polymerase III complex Source: UniProtKB
    4. intracellular membrane-bounded organelle Source: HPA
    5. nucleoplasm Source: UniProtKB
    6. nucleus Source: HPA

    Keywords - Cellular componenti

    DNA-directed RNA polymerase, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134994174.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 204204DNA-directed RNA polymerase III subunit RPC8PRO_0000073994Add
    BLAST

    Proteomic databases

    MaxQBiQ9Y535.
    PaxDbiQ9Y535.
    PRIDEiQ9Y535.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9Y535.
    BgeeiQ9Y535.
    CleanExiHS_POLR3H.
    GenevestigatoriQ9Y535.

    Organism-specific databases

    HPAiHPA046787.

    Interactioni

    Subunit structurei

    Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits By similarity. Interacts with CRCP/RPC9. POLR3H/RPC8 and CRCP/RPC9 probably form a Pol III subcomplex.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi128147. 19 interactions.
    IntActiQ9Y535. 1 interaction.
    STRINGi9606.ENSP00000347345.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Y535.
    SMRiQ9Y535. Positions 1-201.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1095.
    HOGENOMiHOG000157370.
    HOVERGENiHBG044510.
    InParanoidiQ9Y535.
    KOiK03022.
    OMAiNVGLCIA.
    OrthoDBiEOG70CR7V.
    PhylomeDBiQ9Y535.
    TreeFamiTF103053.

    Family and domain databases

    Gene3Di3.30.1490.120. 1 hit.
    InterProiIPR012340. NA-bd_OB-fold.
    IPR013238. RNA_pol_III_Rbc25.
    IPR005576. RNA_pol_Rpb7_N.
    IPR004519. RNAP_E/RPC8.
    [Graphical view]
    PfamiPF08292. RNA_pol_Rbc25. 1 hit.
    PF03876. SHS2_Rpb7-N. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF88798. SSF88798. 1 hit.
    TIGRFAMsiTIGR00448. rpoE. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9Y535-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MFVLVEMVDT VRIPPWQFER KLNDSIAEEL NKKLANKVVY NVGLCICLFD    50
    ITKLEDAYVF PGDGASHTKV HFRCVVFHPF LDEILIGKIK GCSPEGVHVS 100
    LGFFDDILIP PESLQQPAKF DEAEQVWVWE YETEEGAHDL YMDTGEEIRF 150
    RVVDESFVDT SPTGPSSADA TTSSEELPKK EAPYTLVGSI SEPGLGLLSW 200
    WTSN 204
    Length:204
    Mass (Da):22,918
    Last modified:November 1, 1999 - v1
    Checksum:iDE85D44A5770C5D9
    GO
    Isoform 2 (identifier: Q9Y535-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         70-98: Missing.

    Show »
    Length:175
    Mass (Da):19,658
    Checksum:i2213B9D553C5DEE9
    GO

    Sequence cautioni

    The sequence BAB33335.1 differs from that shown. Reason: Erroneous initiation.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei70 – 9829Missing in isoform 2. 1 PublicationVSP_007067Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY092087 mRNA. Translation: AAM18217.1.
    AB051452 mRNA. Translation: BAB33335.1. Different initiation.
    CR456459 mRNA. Translation: CAG30345.1.
    AL023553 Genomic DNA. Translation: CAB46023.1.
    AL023553 Genomic DNA. Translation: CAQ10207.1.
    CH471095 Genomic DNA. Translation: EAW60438.1.
    BC017248 mRNA. Translation: AAH17248.1.
    BC088367 mRNA. Translation: AAH88367.1.
    CCDSiCCDS14018.1. [Q9Y535-1]
    CCDS33651.1. [Q9Y535-2]
    RefSeqiNP_001018060.1. NM_001018050.3. [Q9Y535-1]
    NP_001018062.1. NM_001018052.3. [Q9Y535-2]
    NP_001269813.1. NM_001282884.1. [Q9Y535-1]
    NP_001269814.1. NM_001282885.1. [Q9Y535-1]
    NP_612211.1. NM_138338.4. [Q9Y535-1]
    UniGeneiHs.712617.

    Genome annotation databases

    EnsembliENST00000337566; ENSP00000337627; ENSG00000100413. [Q9Y535-2]
    ENST00000355209; ENSP00000347345; ENSG00000100413. [Q9Y535-1]
    ENST00000396504; ENSP00000379761; ENSG00000100413. [Q9Y535-1]
    ENST00000407461; ENSP00000385315; ENSG00000100413. [Q9Y535-1]
    GeneIDi171568.
    KEGGihsa:171568.
    UCSCiuc003baf.3. human. [Q9Y535-1]
    uc003bai.2. human. [Q9Y535-2]

    Polymorphism databases

    DMDMi29428071.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY092087 mRNA. Translation: AAM18217.1 .
    AB051452 mRNA. Translation: BAB33335.1 . Different initiation.
    CR456459 mRNA. Translation: CAG30345.1 .
    AL023553 Genomic DNA. Translation: CAB46023.1 .
    AL023553 Genomic DNA. Translation: CAQ10207.1 .
    CH471095 Genomic DNA. Translation: EAW60438.1 .
    BC017248 mRNA. Translation: AAH17248.1 .
    BC088367 mRNA. Translation: AAH88367.1 .
    CCDSi CCDS14018.1. [Q9Y535-1 ]
    CCDS33651.1. [Q9Y535-2 ]
    RefSeqi NP_001018060.1. NM_001018050.3. [Q9Y535-1 ]
    NP_001018062.1. NM_001018052.3. [Q9Y535-2 ]
    NP_001269813.1. NM_001282884.1. [Q9Y535-1 ]
    NP_001269814.1. NM_001282885.1. [Q9Y535-1 ]
    NP_612211.1. NM_138338.4. [Q9Y535-1 ]
    UniGenei Hs.712617.

    3D structure databases

    ProteinModelPortali Q9Y535.
    SMRi Q9Y535. Positions 1-201.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 128147. 19 interactions.
    IntActi Q9Y535. 1 interaction.
    STRINGi 9606.ENSP00000347345.

    Polymorphism databases

    DMDMi 29428071.

    Proteomic databases

    MaxQBi Q9Y535.
    PaxDbi Q9Y535.
    PRIDEi Q9Y535.

    Protocols and materials databases

    DNASUi 171568.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000337566 ; ENSP00000337627 ; ENSG00000100413 . [Q9Y535-2 ]
    ENST00000355209 ; ENSP00000347345 ; ENSG00000100413 . [Q9Y535-1 ]
    ENST00000396504 ; ENSP00000379761 ; ENSG00000100413 . [Q9Y535-1 ]
    ENST00000407461 ; ENSP00000385315 ; ENSG00000100413 . [Q9Y535-1 ]
    GeneIDi 171568.
    KEGGi hsa:171568.
    UCSCi uc003baf.3. human. [Q9Y535-1 ]
    uc003bai.2. human. [Q9Y535-2 ]

    Organism-specific databases

    CTDi 171568.
    GeneCardsi GC22M041921.
    HGNCi HGNC:30349. POLR3H.
    HPAi HPA046787.
    neXtProti NX_Q9Y535.
    PharmGKBi PA134994174.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1095.
    HOGENOMi HOG000157370.
    HOVERGENi HBG044510.
    InParanoidi Q9Y535.
    KOi K03022.
    OMAi NVGLCIA.
    OrthoDBi EOG70CR7V.
    PhylomeDBi Q9Y535.
    TreeFami TF103053.

    Enzyme and pathway databases

    Reactomei REACT_1036. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
    REACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
    REACT_347. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
    REACT_571. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
    REACT_63. RNA Polymerase III Transcription Termination.
    REACT_756. RNA Polymerase III Chain Elongation.

    Miscellaneous databases

    GenomeRNAii 171568.
    NextBioi 89296.
    PROi Q9Y535.

    Gene expression databases

    ArrayExpressi Q9Y535.
    Bgeei Q9Y535.
    CleanExi HS_POLR3H.
    Genevestigatori Q9Y535.

    Family and domain databases

    Gene3Di 3.30.1490.120. 1 hit.
    InterProi IPR012340. NA-bd_OB-fold.
    IPR013238. RNA_pol_III_Rbc25.
    IPR005576. RNA_pol_Rpb7_N.
    IPR004519. RNAP_E/RPC8.
    [Graphical view ]
    Pfami PF08292. RNA_pol_Rbc25. 1 hit.
    PF03876. SHS2_Rpb7-N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF88798. SSF88798. 1 hit.
    TIGRFAMsi TIGR00448. rpoE. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits."
      Hu P., Wu S., Sun Y., Yuan C.-C., Kobayashi R., Myers M.P., Hernandez N.
      Mol. Cell. Biol. 22:8044-8055(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1), IDENTIFICATION IN THE RNA POL III COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH RPC9.
    2. "Identification of novel transcribed sequences on human chromosome 22 by expressed sequence tag mapping."
      Hirosawa M., Nagase T., Murahashi Y., Kikuno R., Ohara O.
      DNA Res. 8:1-9(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    4. "The DNA sequence of human chromosome 22."
      Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M.
      , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H.
      Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Brain and Pituitary.
    7. "RNA polymerase III detects cytosolic DNA and induces type I interferons through the RIG-I pathway."
      Chiu Y.-H., Macmillan J.B., Chen Z.J.
      Cell 138:576-591(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate."
      Ablasser A., Bauernfeind F., Hartmann G., Latz E., Fitzgerald K.A., Hornung V.
      Nat. Immunol. 10:1065-1072(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiRPC8_HUMAN
    AccessioniPrimary (citable) accession number: Q9Y535
    Secondary accession number(s): B0QYH9
    , Q5M7Y8, Q96AE3, Q9BY95
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 28, 2003
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 22
      Human chromosome 22: entries, gene names and cross-references to MIM
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3