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Q9Y535 (RPC8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase III subunit RPC8

Short name=RNA polymerase III subunit C8
Alternative name(s):
DNA-directed RNA polymerase III subunit H
RNA polymerase III subunit 22.9 kDa subunit
Short name=RPC22.9
Gene names
Name:POLR3H
Synonyms:KIAA1665, RPC8
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length204 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway By similarity. Ref.7 Ref.8

Subunit structure

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits By similarity. Interacts with CRCP/RPC9. POLR3H/RPC8 and CRCP/RPC9 probably form a Pol III subcomplex. Ref.1

Subcellular location

Nucleus.

Sequence similarities

Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.

Sequence caution

The sequence BAB33335.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processAntiviral defense
Immunity
Innate immunity
Transcription
   Cellular componentDNA-directed RNA polymerase
Nucleus
   Coding sequence diversityAlternative splicing
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdefense response to virus

Inferred from electronic annotation. Source: UniProtKB-KW

gene expression

Traceable author statement. Source: Reactome

innate immune response

Traceable author statement. Source: Reactome

nucleobase-containing compound metabolic process

Inferred from direct assay Ref.1. Source: UniProtKB

positive regulation of type I interferon production

Traceable author statement. Source: Reactome

termination of RNA polymerase III transcription

Traceable author statement. Source: Reactome

transcription elongation from RNA polymerase III promoter

Traceable author statement. Source: Reactome

transcription from RNA polymerase III promoter

Inferred from direct assay Ref.1. Source: UniProtKB

transcription initiation from RNA polymerase III promoter

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Cellular_componentDNA-directed RNA polymerase III complex

Inferred from direct assay Ref.1. Source: UniProtKB

centrosome

Inferred from direct assay. Source: HPA

cytosol

Traceable author statement. Source: Reactome

intracellular membrane-bounded organelle

Inferred from direct assay. Source: HPA

nucleoplasm

Inferred from direct assay Ref.1. Source: UniProtKB

nucleus

Inferred from direct assay. Source: HPA

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

DNA-directed RNA polymerase activity

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Y535-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Y535-2)

The sequence of this isoform differs from the canonical sequence as follows:
     70-98: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 204204DNA-directed RNA polymerase III subunit RPC8
PRO_0000073994

Natural variations

Alternative sequence70 – 9829Missing in isoform 2.
VSP_007067

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: DE85D44A5770C5D9

FASTA20422,918
        10         20         30         40         50         60 
MFVLVEMVDT VRIPPWQFER KLNDSIAEEL NKKLANKVVY NVGLCICLFD ITKLEDAYVF 

        70         80         90        100        110        120 
PGDGASHTKV HFRCVVFHPF LDEILIGKIK GCSPEGVHVS LGFFDDILIP PESLQQPAKF 

       130        140        150        160        170        180 
DEAEQVWVWE YETEEGAHDL YMDTGEEIRF RVVDESFVDT SPTGPSSADA TTSSEELPKK 

       190        200 
EAPYTLVGSI SEPGLGLLSW WTSN 

« Hide

Isoform 2 [UniParc].

Checksum: 2213B9D553C5DEE9
Show »

FASTA17519,658

References

« Hide 'large scale' references
[1]"Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits."
Hu P., Wu S., Sun Y., Yuan C.-C., Kobayashi R., Myers M.P., Hernandez N.
Mol. Cell. Biol. 22:8044-8055(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1), IDENTIFICATION IN THE RNA POL III COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH RPC9.
[2]"Identification of novel transcribed sequences on human chromosome 22 by expressed sequence tag mapping."
Hirosawa M., Nagase T., Murahashi Y., Kikuno R., Ohara O.
DNA Res. 8:1-9(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]"A genome annotation-driven approach to cloning the human ORFeome."
Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I.
Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]"The DNA sequence of human chromosome 22."
Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. expand/collapse author list , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H.
Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain and Pituitary.
[7]"RNA polymerase III detects cytosolic DNA and induces type I interferons through the RIG-I pathway."
Chiu Y.-H., Macmillan J.B., Chen Z.J.
Cell 138:576-591(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate."
Ablasser A., Bauernfeind F., Hartmann G., Latz E., Fitzgerald K.A., Hornung V.
Nat. Immunol. 10:1065-1072(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY092087 mRNA. Translation: AAM18217.1.
AB051452 mRNA. Translation: BAB33335.1. Different initiation.
CR456459 mRNA. Translation: CAG30345.1.
AL023553 Genomic DNA. Translation: CAB46023.1.
AL023553 Genomic DNA. Translation: CAQ10207.1.
CH471095 Genomic DNA. Translation: EAW60438.1.
BC017248 mRNA. Translation: AAH17248.1.
BC088367 mRNA. Translation: AAH88367.1.
RefSeqNP_001018060.1. NM_001018050.3.
NP_001018062.1. NM_001018052.3.
NP_001269813.1. NM_001282884.1.
NP_001269814.1. NM_001282885.1.
NP_612211.1. NM_138338.4.
UniGeneHs.712617.

3D structure databases

ProteinModelPortalQ9Y535.
SMRQ9Y535. Positions 1-201.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid128147. 19 interactions.
IntActQ9Y535. 1 interaction.
STRING9606.ENSP00000347345.

Polymorphism databases

DMDM29428071.

Proteomic databases

PaxDbQ9Y535.
PRIDEQ9Y535.

Protocols and materials databases

DNASU171568.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000337566; ENSP00000337627; ENSG00000100413. [Q9Y535-2]
ENST00000355209; ENSP00000347345; ENSG00000100413. [Q9Y535-1]
ENST00000396504; ENSP00000379761; ENSG00000100413. [Q9Y535-1]
ENST00000407461; ENSP00000385315; ENSG00000100413. [Q9Y535-1]
GeneID171568.
KEGGhsa:171568.
UCSCuc003baf.3. human. [Q9Y535-1]
uc003bai.2. human. [Q9Y535-2]

Organism-specific databases

CTD171568.
GeneCardsGC22M041921.
HGNCHGNC:30349. POLR3H.
HPAHPA046787.
neXtProtNX_Q9Y535.
PharmGKBPA134994174.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1095.
HOGENOMHOG000157370.
HOVERGENHBG044510.
InParanoidQ9Y535.
KOK03022.
OMANVGLCIA.
OrthoDBEOG70CR7V.
PhylomeDBQ9Y535.
TreeFamTF103053.

Enzyme and pathway databases

ReactomeREACT_1788. Transcription.
REACT_6900. Immune System.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpressQ9Y535.
BgeeQ9Y535.
CleanExHS_POLR3H.
GenevestigatorQ9Y535.

Family and domain databases

Gene3D3.30.1490.120. 1 hit.
InterProIPR012340. NA-bd_OB-fold.
IPR013238. RNA_pol_III_Rbc25.
IPR005576. RNA_pol_Rpb7_N.
IPR004519. RNAP_E/RPC8.
[Graphical view]
PfamPF08292. RNA_pol_Rbc25. 1 hit.
PF03876. SHS2_Rpb7-N. 1 hit.
[Graphical view]
SUPFAMSSF50249. SSF50249. 1 hit.
SSF88798. SSF88798. 1 hit.
TIGRFAMsTIGR00448. rpoE. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi171568.
NextBio89296.
PROQ9Y535.

Entry information

Entry nameRPC8_HUMAN
AccessionPrimary (citable) accession number: Q9Y535
Secondary accession number(s): B0QYH9 expand/collapse secondary AC list , Q5M7Y8, Q96AE3, Q9BY95
Entry history
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: November 1, 1999
Last modified: April 16, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 22

Human chromosome 22: entries, gene names and cross-references to MIM