Q9Y4X4 (KLF12_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Krueppel-like factor 12

Alternative name(s):
Transcriptional repressor AP-2rep

Gene names

Name:	KLF12
Synonyms:	AP2REP
ORF Names:	HSPC122

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Confers strong transcriptional repression to the AP-2-alpha gene. Binds to a regulatory element (A32) in the AP-2-alpha gene promoter.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the Sp1 C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Repressor
PTM	Methylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 16615998. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from direct assay PubMed 16615998. Source: MGI sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 16615998. Source: MGI transcription corepressor activity Traceable author statement Ref.1. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y4X4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y4X4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     270-402: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 402	402	Krueppel-like factor 12		PRO_0000047182
Regions
	Zinc finger	317 – 341	25	C2H2-type 1
	Zinc finger	347 – 371	25	C2H2-type 2
	Zinc finger	377 – 399	23	C2H2-type 3
Amino acid modifications
	Modified residue	202	1	Phosphoserine Ref.10
	Modified residue	313	1	N6-methylated lysine; by EHMT2 Ref.9
Natural variations
	Alternative sequence	270 – 402	133	Missing in isoform 2.		VSP_006876
Experimental info
	Sequence conflict	246	1	P → L in CAB46982. Ref.1
	Sequence conflict	246	1	P → L in AAK12082. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 7, 2006. Version 2. Checksum: 9557E776878BAA8D	FASTA	402	44,240
	10 20 30 40 50 60 MNIHMKRKTI KNINTFENRM LMLDGMPAVR VKTELLESEQ GSPNVHNYPD MEAVPLLLNN 70 80 90 100 110 120 VKGEPPEDSL SVDHFQTQTE PVDLSINKAR TSPTAVSSSP VSMTASASSP SSTSTSSSSS 130 140 150 160 170 180 SRLASSPTVI TSVSSASSSS TVLTPGPLVA SASGVGGQQF LHIIHPVPPS SPMNLQSNKL 190 200 210 220 230 240 SHVHRIPVVV QSVPVVYTAV RSPGNVNNTI VVPLLEDGRG HGKAQMDPRG LSPRQSKSDS 250 260 270 280 290 300 DDDDLPNVTL DSVNETGSTA LSIARAVQEV HPSPVSRVRG NRMNNQKFPC SISPFSIEST 310 320 330 340 350 360 RRQRRSESPD SRKRRIHRCD FEGCNKVYTK SSHLKAHRRT HTGEKPYKCT WEGCTWKFAR 370 380 390 400 SDELTRHYRK HTGVKPFKCA DCDRSFSRSD HLALHRRRHM LV
	Isoform 2 [UniParc]. Checksum: 6026ED58AB6AA023	FASTA	269	28,407
	10 20 30 40 50 60 MNIHMKRKTI KNINTFENRM LMLDGMPAVR VKTELLESEQ GSPNVHNYPD MEAVPLLLNN 70 80 90 100 110 120 VKGEPPEDSL SVDHFQTQTE PVDLSINKAR TSPTAVSSSP VSMTASASSP SSTSTSSSSS 130 140 150 160 170 180 SRLASSPTVI TSVSSASSSS TVLTPGPLVA SASGVGGQQF LHIIHPVPPS SPMNLQSNKL 190 200 210 220 230 240 SHVHRIPVVV QSVPVVYTAV RSPGNVNNTI VVPLLEDGRG HGKAQMDPRG LSPRQSKSDS 250 260 DDDDLPNVTL DSVNETGSTA LSIARAVQE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic structure and DNA binding properties of the human zinc finger transcriptional repressor AP-2rep (KLF12)." Roth C., Schuierer M., Gunther K., Buettner R. Genomics 63:384-390(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Defining a common region of deletion at 13q21 in human cancers." Chen C., Brabham W.W., Stultz B.G., Frierson H.F. Jr., Barrett J.C., Sawyers C.L., Isaacs J.T., Dong J.T. Genes Chromosomes Cancer 31:333-344(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A novel gene expressed in the human hypothalamus." Jiang C., Peng Y., Gu J., Gu Y., Fu S., Wu T., Dong H., Jin W., Fu G., Han Z., Chen Z., Wang Y. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Hypothalamus.
[4]	"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. , Gu J., Chen S.-J., Chen Z. Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Umbilical cord blood.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[6]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[9]	"Protein lysine methyltransferase G9a acts on non-histone targets." Rathert P., Dhayalan A., Murakami M., Zhang X., Tamas R., Jurkowska R., Komatsu Y., Shinkai Y., Cheng X., Jeltsch A. Nat. Chem. Biol. 4:344-346(2008) [PubMed] [Europe PMC] [Abstract] Cited for: METHYLATION AT LYS-313.
[10]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[11]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ243274 mRNA. Translation: CAB46982.1. AF312872 , AF312866, AF312867, AF312868, AF312869, AF312870, AF312871 Genomic DNA. Translation: AAK12082.1. AF113122 mRNA. Translation: AAF14863.1. AF161471 mRNA. Translation: AAF29086.1. AK291397 mRNA. Translation: BAF84086.1. AL159972 , AL138713, AL139036, AL160032 Genomic DNA. Translation: CAH70670.1. AL139036 , AL138713, AL159972, AL160032 Genomic DNA. Translation: CAH71998.1. AL160032 , AL138713, AL139036, AL159972 Genomic DNA. Translation: CAH74171.1. AL138713 , AL139036, AL159972, AL160032 Genomic DNA. Translation: CAI15634.1. CH471093 Genomic DNA. Translation: EAW80529.1. BC019680 mRNA. Translation: AAH19680.1.
IPI	IPI00027024. IPI00239722.
RefSeq	NP_009180.3. NM_007249.4.
UniGene	Hs.373857. Hs.729350.
3D structure databases
ProteinModelPortal	Q9Y4X4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y4X4. 1 interaction.
MINT	MINT-199288.
STRING	9606.ENSP00000366894.
PTM databases
PhosphoSite	Q9Y4X4.
Polymorphism databases
DMDM	91771555.
Proteomic databases
PaxDb	Q9Y4X4.
PRIDE	Q9Y4X4.
Protocols and materials databases
DNASU	11278.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000377666; ENSP00000366894; ENSG00000118922. ENST00000377669; ENSP00000366897; ENSG00000118922.
GeneID	11278.
KEGG	hsa:11278.
UCSC	uc001vjf.3. human.
Organism-specific databases
CTD	11278.
GeneCards	GC13M074260.
HGNC	HGNC:6346. KLF12.
MIM	607531. gene.
neXtProt	NX_Q9Y4X4.
PharmGKB	PA30132.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5048.
HOGENOM	HOG000232138.
HOVERGEN	HBG003941.
KO	K09205.
OrthoDB	EOG4Z62P0.
PhylomeDB	Q9Y4X4.
Gene expression databases
Bgee	Q9Y4X4.
CleanEx	HS_KLF12.
Genevestigator	Q9Y4X4.
GermOnline	ENSG00000118922. Homo sapiens.
Family and domain databases
Gene3D	3.30.160.60. 3 hits.
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
SMART	SM00355. ZnF_C2H2. 3 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 3 hits. PS50157. ZINC_FINGER_C2H2_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	KLF12. human.
GenomeRNAi	11278.
NextBio	42933.
SOURCE	Search...

Entry information

Entry name

KLF12_HUMAN

Accession

Primary (citable) accession number: Q9Y4X4
Secondary accession number(s): A8K5T2, Q5VZM7, Q9UHZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2001
Last sequence update:	March 7, 2006
Last modified:	May 1, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.