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Q9Y4X4 (KLF12_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Krueppel-like factor 12
Alternative name(s):
Transcriptional repressor AP-2rep
Gene names
Name:KLF12
Synonyms:AP2REP
ORF Names:HSPC122
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length402 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Confers strong transcriptional repression to the AP-2-alpha gene. Binds to a regulatory element (A32) in the AP-2-alpha gene promoter.

Subcellular location

Nucleus.

Sequence similarities

Belongs to the Sp1 C2H2-type zinc-finger protein family.

Contains 3 C2H2-type zinc fingers.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionRepressor
   PTMMethylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processnegative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 16615998. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

regulation of transcription from RNA polymerase II promoter

Traceable author statement Ref.1. Source: ProtInc

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from direct assay PubMed 16615998. Source: MGI

RNA polymerase II regulatory region sequence-specific DNA binding

Inferred from electronic annotation. Source: Ensembl

RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription

Inferred from electronic annotation. Source: Ensembl

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 16615998. Source: MGI

transcription corepressor activity

Traceable author statement Ref.1. Source: ProtInc

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Y4X4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Y4X4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     270-402: Missing.
Isoform 3 (identifier: Q9Y4X4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     290-309: CSISPFSIESTRRQRRSESP → WRETLQVYLGRLHLEVRSFR
     310-402: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 402402Krueppel-like factor 12
PRO_0000047182

Regions

Zinc finger317 – 34125C2H2-type 1
Zinc finger347 – 37125C2H2-type 2
Zinc finger377 – 39923C2H2-type 3

Amino acid modifications

Modified residue2021Phosphoserine Ref.11
Modified residue3131N6-methylated lysine; by EHMT2 Ref.10

Natural variations

Alternative sequence270 – 402133Missing in isoform 2.
VSP_006876
Alternative sequence290 – 30920CSISP…RSESP → WRETLQVYLGRLHLEVRSFR in isoform 3.
VSP_047486
Alternative sequence310 – 40293Missing in isoform 3.
VSP_047487

Experimental info

Sequence conflict2461P → L in CAB46982. Ref.1
Sequence conflict2461P → L in AAK12082. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 7, 2006. Version 2.
Checksum: 9557E776878BAA8D

FASTA40244,240
        10         20         30         40         50         60 
MNIHMKRKTI KNINTFENRM LMLDGMPAVR VKTELLESEQ GSPNVHNYPD MEAVPLLLNN 

        70         80         90        100        110        120 
VKGEPPEDSL SVDHFQTQTE PVDLSINKAR TSPTAVSSSP VSMTASASSP SSTSTSSSSS 

       130        140        150        160        170        180 
SRLASSPTVI TSVSSASSSS TVLTPGPLVA SASGVGGQQF LHIIHPVPPS SPMNLQSNKL 

       190        200        210        220        230        240 
SHVHRIPVVV QSVPVVYTAV RSPGNVNNTI VVPLLEDGRG HGKAQMDPRG LSPRQSKSDS 

       250        260        270        280        290        300 
DDDDLPNVTL DSVNETGSTA LSIARAVQEV HPSPVSRVRG NRMNNQKFPC SISPFSIEST 

       310        320        330        340        350        360 
RRQRRSESPD SRKRRIHRCD FEGCNKVYTK SSHLKAHRRT HTGEKPYKCT WEGCTWKFAR 

       370        380        390        400 
SDELTRHYRK HTGVKPFKCA DCDRSFSRSD HLALHRRRHM LV 

« Hide

Isoform 2 [UniParc].

Checksum: 6026ED58AB6AA023
Show »

FASTA26928,407
Isoform 3 [UniParc].

Checksum: 9D3AA6F9D80D2AE4
Show »

FASTA30933,250

References

« Hide 'large scale' references
[1]"Genomic structure and DNA binding properties of the human zinc finger transcriptional repressor AP-2rep (KLF12)."
Roth C., Schuierer M., Gunther K., Buettner R.
Genomics 63:384-390(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Defining a common region of deletion at 13q21 in human cancers."
Chen C., Brabham W.W., Stultz B.G., Frierson H.F. Jr., Barrett J.C., Sawyers C.L., Isaacs J.T., Dong J.T.
Genes Chromosomes Cancer 31:333-344(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Shaking the family tree: Identification of novel and biologically active alternatively spliced isoforms across the KLF family of transcription factors."
Camacho-Vanegas O., Till J., Miranda-Lorenzo I., Ozturk B., Camacho S.C., Martignetti J.A.
FASEB J. 27:432-436(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), ALTERNATIVE SPLICING.
[4]"A novel gene expressed in the human hypothalamus."
Jiang C., Peng Y., Gu J., Gu Y., Fu S., Wu T., Dong H., Jin W., Fu G., Han Z., Chen Z., Wang Y.
Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Hypothalamus.
[5]"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. expand/collapse author list , Gu J., Chen S.-J., Chen Z.
Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Umbilical cord blood.
[6]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[7]"The DNA sequence and analysis of human chromosome 13."
Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[10]"Protein lysine methyltransferase G9a acts on non-histone targets."
Rathert P., Dhayalan A., Murakami M., Zhang X., Tamas R., Jurkowska R., Komatsu Y., Shinkai Y., Cheng X., Jeltsch A.
Nat. Chem. Biol. 4:344-346(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: METHYLATION AT LYS-313.
[11]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[12]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ243274 mRNA. Translation: CAB46982.1.
AF312872 expand/collapse EMBL AC list , AF312866, AF312867, AF312868, AF312869, AF312870, AF312871 Genomic DNA. Translation: AAK12082.1.
HF546212 mRNA. Translation: CCO02798.1.
AF113122 mRNA. Translation: AAF14863.1.
AF161471 mRNA. Translation: AAF29086.1.
AK291397 mRNA. Translation: BAF84086.1.
AL159972 expand/collapse EMBL AC list , AL138713, AL139036, AL160032 Genomic DNA. Translation: CAH70670.1.
AL139036 expand/collapse EMBL AC list , AL138713, AL159972, AL160032 Genomic DNA. Translation: CAH71998.1.
AL160032 expand/collapse EMBL AC list , AL138713, AL139036, AL159972 Genomic DNA. Translation: CAH74171.1.
AL138713 expand/collapse EMBL AC list , AL139036, AL159972, AL160032 Genomic DNA. Translation: CAI15634.1.
CH471093 Genomic DNA. Translation: EAW80529.1.
BC019680 mRNA. Translation: AAH19680.1.
RefSeqNP_009180.3. NM_007249.4.
XP_005266308.1. XM_005266251.2.
XP_005266309.1. XM_005266252.2.
UniGeneHs.373857.
Hs.729350.

3D structure databases

ProteinModelPortalQ9Y4X4.
SMRQ9Y4X4. Positions 306-402.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116434. 3 interactions.
IntActQ9Y4X4. 1 interaction.
MINTMINT-199288.
STRING9606.ENSP00000366894.

PTM databases

PhosphoSiteQ9Y4X4.

Polymorphism databases

DMDM91771555.

Proteomic databases

PaxDbQ9Y4X4.
PRIDEQ9Y4X4.

Protocols and materials databases

DNASU11278.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000377666; ENSP00000366894; ENSG00000118922. [Q9Y4X4-1]
ENST00000377669; ENSP00000366897; ENSG00000118922. [Q9Y4X4-1]
GeneID11278.
KEGGhsa:11278.
UCSCuc001vjf.3. human. [Q9Y4X4-1]

Organism-specific databases

CTD11278.
GeneCardsGC13M074260.
HGNCHGNC:6346. KLF12.
MIM607531. gene.
neXtProtNX_Q9Y4X4.
PharmGKBPA30132.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5048.
HOGENOMHOG000232138.
HOVERGENHBG003941.
KOK09205.
OMASLPLMYT.
OrthoDBEOG747PJ4.
PhylomeDBQ9Y4X4.
TreeFamTF350556.

Enzyme and pathway databases

SignaLinkQ9Y4X4.

Gene expression databases

BgeeQ9Y4X4.
CleanExHS_KLF12.
GenevestigatorQ9Y4X4.

Family and domain databases

Gene3D3.30.160.60. 3 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSKLF12. human.
GeneWikiKLF12.
GenomeRNAi11278.
NextBio42933.
PROQ9Y4X4.
SOURCESearch...

Entry information

Entry nameKLF12_HUMAN
AccessionPrimary (citable) accession number: Q9Y4X4
Secondary accession number(s): A8K5T2 expand/collapse secondary AC list , L0R3J4, Q5VZM7, Q9UHZ0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: March 7, 2006
Last modified: April 16, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 13

Human chromosome 13: entries, gene names and cross-references to MIM