Reviewed, UniProtKB/Swiss-Prot Q9Y4W6 (AFG32_HUMAN)

Names and origin

Protein names

Recommended name:
    AFG3-like protein 2
    EC=3.4.24.-
Alternative name(s):
    Paraplegin-like protein

Gene names

Name:

AFG3L2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	797 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Putative ATP-dependent protease.
Cofactor	Binds 1 zinc ion per subunit Potential.
Subcellular location	Mitochondrion membrane; Multi-pass membrane protein.
Tissue specificity	Ubiquitous.
Sequence similarities	In the N-terminal section; belongs to the AAA ATPase family. In the C-terminal section; belongs to the peptidase M41 family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Domain	Transmembrane
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro unfolded protein binding Ref.1 Traceable author statement. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 797	797	AFG3-like protein 2		PRO_0000084673
Regions
	Transmembrane	143 – 163	21	Potential
	Transmembrane	251 – 271	21	Potential
	Nucleotide binding	348 – 355	8	ATP Potential
Sites
	Active site	575	1	By similarity
	Metal binding	574	1	Zinc; catalytic By similarity
	Metal binding	578	1	Zinc; catalytic By similarity
Amino acid modifications
	Modified residue	306	1	N6-acetyllysine Ref.4
	Modified residue	308	1	N6-acetyllysine By similarity
	Modified residue	543	1	N6-acetyllysine Ref.4
Experimental info
	Sequence conflict	74	1	E → G in CAB48398. Ref.1
	Sequence conflict	633	1	V → A in CAB48398. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9Y4W6-1 [UniParc]. Last modified February 20, 2007. Version 2. Checksum: EACBB7C5F2EE5E08	FASTA	797	88,584
	10 20 30 40 50 60 MAHRCLRLWG RGGCWPRGLQ QLLVPGGVGP GEQPCLRTLY RFVTTQARAS RNSLLTDIIA 70 80 90 100 110 120 AYQRFCSRPP KGFEKYFPNG KNGKKASEPK EVMGEKKESK PAATTRSSGG GGGGGGKRGG 130 140 150 160 170 180 KKDDSHWWSR FQKGDIPWDD KDFRMFFLWT ALFWGGVMFY LLLKRSGREI TWKDFVNNYL 190 200 210 220 230 240 SKGVVDRLEV VNKRFVRVTF TPGKTPVDGQ YVWFNIGSVD TFERNLETLQ QELGIEGENR 250 260 270 280 290 300 VPVVYIAESD GSFLLSMLPT VLIIAFLLYT IRRGPAGIGR TGRGMGGLFS VGETTAKVLK 310 320 330 340 350 360 DEIDVKFKDV AGCEEAKLEI MEFVNFLKNP KQYQDLGAKI PKGAILTGPP GTGKTLLAKA 370 380 390 400 410 420 TAGEANVPFI TVSGSEFLEM FVGVGPARVR DLFALARKNA PCILFIDEID AVGRKRGRGN 430 440 450 460 470 480 FGGQSEQENT LNQLLVEMDG FNTTTNVVIL AGTNRPDILD PALLRPGRFD RQIFIGPPDI 490 500 510 520 530 540 KGRASIFKVH LRPLKLDSTL EKDKLARKLA SLTPGFSGAD VANVCNEAAL IAARHLSDSI 550 560 570 580 590 600 NQKHFEQAIE RVIGGLEKKT QVLQPEEKKT VAYHEAGHAV AGWYLEHADP LLKVSIIPRG 610 620 630 640 650 660 KGLGYAQYLP KEQYLYTKEQ LLDRMCMTLG GRVSEEIFFG RITTGAQDDL RKVTQSAYAQ 670 680 690 700 710 720 IVQFGMNEKV GQISFDLPRQ GDMVLEKPYS EATARLIDDE VRILINDAYK RTVALLTEKK 730 740 750 760 770 780 ADVEKVALLL LEKEVLDKND MVELLGPRPF AEKSTYEEFV EGTGSLDEDT SLPEGLKDWN 790 KEREKEKEEP PGEKVAN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of AFG3L2, a novel paraplegin-related gene." Banfi S., Bassi M.T., Andolfi G., Marchitiello A., Zanotta S., Ballabio A., Casari G., Franco B. Genomics 59:51-58(1999) [PubMed: 10395799] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.
[3]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[4]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-306 AND LYS-543, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y18314 mRNA. Translation: CAB48398.1. BC065016 mRNA. Translation: AAH65016.1.
IPI	IPI00001091.
RefSeq	NP_006787.1.
UniGene	Hs.528996
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y4W6. 2 interactions.
STRING	Q9Y4W6.
Protein family/group databases
MEROPS	M41.007.
PTM databases
PhosphoSite	Q9Y4W6.
Proteomic databases
PeptideAtlas	Q9Y4W6.
PRIDE	Q9Y4W6.
Genome annotation databases
Ensembl	ENST00000269143; ENSP00000269143; ENSG00000141385; Homo sapiens. [Genome view]
GeneID	10939.
KEGG	hsa:10939.
UCSC	uc002kqz.1. human.
Organism-specific databases
CTD	10939.
GeneCards	GC18M012319.
H-InvDB	HIX0027367.
HGNC	HGNC:315. AFG3L2.
HPA	HPA004479. HPA004480.
MIM	604581. gene.
PharmGKB	PA24612.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q9Y4W6.
OMA	LNGKKPN
OrthoDB	EOG9QNQG0
Gene expression databases
ArrayExpress	Q9Y4W6.
Bgee	Q9Y4W6.
CleanEx	HS_AFG3L2.
Genevestigator	Q9Y4W6.
GermOnline	ENSG00000141385. Homo sapiens.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR005936. Pept_M41_FtsH. IPR011546. Pept_M41_FtsH_extracell. IPR000642. Peptidase_M41. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF06480. FtsH_ext. 1 hit. PF01434. Peptidase_M41. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01241. FtsH_fam. 1 hit.
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB00171. Adenosine triphosphate.
NextBio	41551.
SOURCE	Search...

Entry information

Entry name

AFG32_HUMAN

Accession

Primary (citable) accession number: Q9Y4W6
Secondary accession number(s): Q6P1L0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	February 20, 2007
Last modified:	November 24, 2009
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.