Reviewed, UniProtKB/Swiss-Prot Q9Y4W2 (LAS1L_HUMAN)

Names and origin

Protein names

Recommended name:
    LAS1-like protein

Gene names

Name:	LAS1L
ORF Names:	MSTP060

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	734 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Nucleus › nucleolus. Ref.6
Sequence similarities	Belongs to the LAS1 family.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PNKP	Q96T60	1	EBI-1051591,EBI-1045072

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y4W2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y4W2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     348-365: DGQTEVQRGEGTDPKSHK → E

Isoform 3 (identifier: Q9Y4W2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     79-120: Missing.
     348-365: DGQTEVQRGEGTDPKSHK → E

Note: Gene prediction based on EST data. No experimental confirmation available.

Isoform 4 (identifier: Q9Y4W2-4)

The sequence of this isoform differs from the canonical sequence as follows:
     283-291: VLEKFRYLP → GGCAGCFSG
     292-734: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 734	734	LAS1-like protein		PRO_0000211559
Amino acid modifications
	Modified residue	523	1	Phosphoserine Ref.7 Ref.8
Natural variations
	Alternative sequence	79 – 120	42	Missing in isoform 3.		VSP_015178
	Alternative sequence	283 – 291	9	VLEKFRYLP → GGCAGCFSG in isoform 4.		VSP_015179
	Alternative sequence	292 – 734	443	Missing in isoform 4.		VSP_015180
	Alternative sequence	348 – 365	18	DGQTE…PKSHK → E in isoform 2 and isoform 3.		VSP_015181
	Natural variant	170	1	R → C in a colorectal cancer sample; somatic mutation. Ref.10		VAR_036587

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 30, 2005. Version 2. Checksum: A35CC38F95C39F7D	FASTA	734	83,065
	10 20 30 40 50 60 MSWESGAGPG LGSQGMDLVW SAWYGKCVKG KGSLPLSAHG IVVAWLSRAE WDQVTVYLFC 70 80 90 100 110 120 DDHKLQRYAL NRITVWRSRS GNELPLAVAS TADLIRCKLL DVTGGLGTDE LRLLYGMALV 130 140 150 160 170 180 RFVNLISERK TKFAKVPLKC LAQEVNIPDW IVDLRHELTH KKMPHINDCR RGCYFVLDWL 190 200 210 220 230 240 QKTYWCRQLE NSLRETWELE EFREGIEEED QEEDKNIVVD DITEQKPEPQ DDGKSTESDV 250 260 270 280 290 300 KADGDSKGSE EVDSHCKKAL SHKELYERAR ELLVSYEEEQ FTVLEKFRYL PKAIKAWNNP 310 320 330 340 350 360 SPRVECVLAE LKGVTCENRE AVLDAFLDDG FLVPTFEQLA ALQIEYEDGQ TEVQRGEGTD 370 380 390 400 410 420 PKSHKNVDLN DVLVPKPFSQ FWQPLLRGLH SQNFTQALLE RMLSELPALG ISGIRPTYIL 430 440 450 460 470 480 RWTVELIVAN TKTGRNARRF SAGQWEARRG WRLFNCSASL DWPRMVESCL GSPCWASPQL 490 500 510 520 530 540 LRIIFKAMGQ GLPDEEQEKL LRICSIYTQS GENSLVQEGS EASPIGKSPY TLDSLYWSVK 550 560 570 580 590 600 PASSSFGSEA KAQQQEEQGS VNDVKEEEKE EKEVLPDQVE EEEENDDQEE EEEDEDDEDD 610 620 630 640 650 660 EEEDRMEVGP FSTGQESPTA ENARLLAQKR GALQGSAWQV SSEDVRWDTF PLGRMPGQTE 670 680 690 700 710 720 DPAELMLENY DTMYLLDQPV LEQRLEPSTC KTDTLGLSCG VGSGNCSNSS SSNFEGLLWS 730 QGQLHGLKTG LQLF
	Isoform 2. Checksum: 651B7F0FFB3FD07F	FASTA	717	81,243
	10 20 30 40 50 60 MSWESGAGPG LGSQGMDLVW SAWYGKCVKG KGSLPLSAHG IVVAWLSRAE WDQVTVYLFC 70 80 90 100 110 120 DDHKLQRYAL NRITVWRSRS GNELPLAVAS TADLIRCKLL DVTGGLGTDE LRLLYGMALV 130 140 150 160 170 180 RFVNLISERK TKFAKVPLKC LAQEVNIPDW IVDLRHELTH KKMPHINDCR RGCYFVLDWL 190 200 210 220 230 240 QKTYWCRQLE NSLRETWELE EFREGIEEED QEEDKNIVVD DITEQKPEPQ DDGKSTESDV 250 260 270 280 290 300 KADGDSKGSE EVDSHCKKAL SHKELYERAR ELLVSYEEEQ FTVLEKFRYL PKAIKAWNNP 310 320 330 340 350 360 SPRVECVLAE LKGVTCENRE AVLDAFLDDG FLVPTFEQLA ALQIEYEENV DLNDVLVPKP 370 380 390 400 410 420 FSQFWQPLLR GLHSQNFTQA LLERMLSELP ALGISGIRPT YILRWTVELI VANTKTGRNA 430 440 450 460 470 480 RRFSAGQWEA RRGWRLFNCS ASLDWPRMVE SCLGSPCWAS PQLLRIIFKA MGQGLPDEEQ 490 500 510 520 530 540 EKLLRICSIY TQSGENSLVQ EGSEASPIGK SPYTLDSLYW SVKPASSSFG SEAKAQQQEE 550 560 570 580 590 600 QGSVNDVKEE EKEEKEVLPD QVEEEEENDD QEEEEEDEDD EDDEEEDRME VGPFSTGQES 610 620 630 640 650 660 PTAENARLLA QKRGALQGSA WQVSSEDVRW DTFPLGRMPG QTEDPAELML ENYDTMYLLD 670 680 690 700 710 QPVLEQRLEP STCKTDTLGL SCGVGSGNCS NSSSSNFEGL LWSQGQLHGL KTGLQLF
	Isoform 3. Checksum: EC904EABA17F0028	FASTA	675	76,845
	10 20 30 40 50 60 MSWESGAGPG LGSQGMDLVW SAWYGKCVKG KGSLPLSAHG IVVAWLSRAE WDQVTVYLFC 70 80 90 100 110 120 DDHKLQRYAL NRITVWRSRF VNLISERKTK FAKVPLKCLA QEVNIPDWIV DLRHELTHKK 130 140 150 160 170 180 MPHINDCRRG CYFVLDWLQK TYWCRQLENS LRETWELEEF REGIEEEDQE EDKNIVVDDI 190 200 210 220 230 240 TEQKPEPQDD GKSTESDVKA DGDSKGSEEV DSHCKKALSH KELYERAREL LVSYEEEQFT 250 260 270 280 290 300 VLEKFRYLPK AIKAWNNPSP RVECVLAELK GVTCENREAV LDAFLDDGFL VPTFEQLAAL 310 320 330 340 350 360 QIEYEENVDL NDVLVPKPFS QFWQPLLRGL HSQNFTQALL ERMLSELPAL GISGIRPTYI 370 380 390 400 410 420 LRWTVELIVA NTKTGRNARR FSAGQWEARR GWRLFNCSAS LDWPRMVESC LGSPCWASPQ 430 440 450 460 470 480 LLRIIFKAMG QGLPDEEQEK LLRICSIYTQ SGENSLVQEG SEASPIGKSP YTLDSLYWSV 490 500 510 520 530 540 KPASSSFGSE AKAQQQEEQG SVNDVKEEEK EEKEVLPDQV EEEEENDDQE EEEEDEDDED 550 560 570 580 590 600 DEEEDRMEVG PFSTGQESPT AENARLLAQK RGALQGSAWQ VSSEDVRWDT FPLGRMPGQT 610 620 630 640 650 660 EDPAELMLEN YDTMYLLDQP VLEQRLEPST CKTDTLGLSC GVGSGNCSNS SSSNFEGLLW 670 SQGQLHGLKT GLQLF
	Isoform 4. Checksum: 500B863F92E96866	FASTA	291	33,108
	10 20 30 40 50 60 MSWESGAGPG LGSQGMDLVW SAWYGKCVKG KGSLPLSAHG IVVAWLSRAE WDQVTVYLFC 70 80 90 100 110 120 DDHKLQRYAL NRITVWRSRS GNELPLAVAS TADLIRCKLL DVTGGLGTDE LRLLYGMALV 130 140 150 160 170 180 RFVNLISERK TKFAKVPLKC LAQEVNIPDW IVDLRHELTH KKMPHINDCR RGCYFVLDWL 190 200 210 220 230 240 QKTYWCRQLE NSLRETWELE EFREGIEEED QEEDKNIVVD DITEQKPEPQ DDGKSTESDV 250 260 270 280 290 KADGDSKGSE EVDSHCKKAL SHKELYERAR ELLVSYEEEQ FTGGCAGCFS G

References

[1]	Zhao B., Xu Y.Y., Liu Y.Q., Wang X.Y., Liu B., Ye J., Song L., Zhao Y., Cao H.Q., Zhao X.W., Gao Y., Liu L.S., Ding J.F., Gao R.L., Wu Q.Y., Qiang B.Q., Yuan J.G., Liew C.C., Zhao M.S., Hui R.T. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Aorta.
[2]	"The nucleotide sequence of a long cDNA clone isolated from human spleen." Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Spleen.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Teratocarcinoma.
[4]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed: 15772651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Bone marrow, Lung and Placenta.
[6]	"Functional proteomic analysis of human nucleolus." Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J. Mol. Biol. Cell 13:4100-4109(2002) [PubMed: 12429849] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-523, MASS SPECTROMETRY. Tissue: Epithelium.
[8]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-523, MASS SPECTROMETRY.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[10]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] CYS-170.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF116730 mRNA. Translation: AAO15306.1. AK074087 mRNA. Translation: BAB84913.1. Different initiation. AK022587 mRNA. Translation: BAB14114.1. AL050306 Genomic DNA. Translation: CAB51351.1. AL050306 Genomic DNA. Translation: CAI42835.1. AL050306 Genomic DNA. Translation: CAI42836.1. BC014545 mRNA. Translation: AAH14545.1. BC018610 mRNA. Translation: AAH18610.1. BC019302 mRNA. Translation: AAH19302.1.
IPI	IPI00009917. IPI00152781. IPI00641990. IPI00645869.
RefSeq	NP_112483.1.
UniGene	Hs.522675
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y4W2. 3 interactions.
PTM databases
PhosphoSite	Q9Y4W2.
2-D gel databases
SWISS-2DPAGE	Q9Y4W2.
Proteomic databases
PRIDE	Q9Y4W2.
Genome annotation databases
Ensembl	ENSG00000001497. Homo sapiens. [Contig view]
GeneID	81887.
KEGG	hsa:81887.
UCSC	uc004dwa.1. human. uc004dwc.1. human. uc004dwd.1. human.
Organism-specific databases
GeneCards	GC0XM064649.
H-InvDB	HIX0016841.
HGNC	HGNC:25726. LAS1L.
PharmGKB	PA128394732.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9Y4W2.
HOVERGEN	Q9Y4W2.
OMA	Q9Y4W2. SPCWASP.
Gene expression databases
ArrayExpress	Q9Y4W2.
Bgee	Q9Y4W2.
CleanEx	HS_LAS1L.
GermOnline	ENSG00000001497. Homo sapiens.
Family and domain databases
InterPro	IPR007174. Las1. [Graphical view]
PANTHER	PTHR15002. Las1. 1 hit.
Pfam	PF04031. Las1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	72228.

Entry information

Entry name

LAS1L_HUMAN

Accession

Primary (citable) accession number: Q9Y4W2
Secondary accession number(s): Q5JXQ0, Q8TEN5, Q9H9V5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	August 30, 2005
Last modified:	July 7, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)