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Protein

E3 ubiquitin-protein ligase RNF115

Gene

RNF115

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates E2-dependent, 'Lys-48'- and/or 'Lys-63'-linked polyubiquitination of substrates and may play a role in diverse biological processes. Through their polyubiquitination, may play a role in the endosomal trafficking and degradation of membrane receptors including EGFR, FLT3, MET and CXCR4.1 Publication2 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri228 – 269RING-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF115Curated (EC:2.3.2.271 Publication)
Alternative name(s):
RING finger protein 115Imported
RING-type E3 ubiquitin transferase RNF115Curated
Rabring 7By similarity
Zinc finger protein 3641 Publication
Gene namesi
Name:RNF115Imported
Synonyms:ZNF3641 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:18154. RNF115.

Subcellular locationi

  • Cytoplasmcytosol By similarity

  • Note: The GTP-bound form of RAB7A recruits RNF115 from the cytosol onto late endosomes/lysosomes.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi26K → R: Loss of autoubiquitination; when associated with R-32. 1 Publication1
Mutagenesisi32K → R: Loss of autoubiquitination; when associated with R-26. 1 Publication1
Mutagenesisi228C → A: Loss of autoubiquitination. 1 Publication1
Mutagenesisi231C → A: Loss of autoubiquitination. 1 Publication1

Organism-specific databases

DisGeNETi27246.
OpenTargetsiENSG00000265491.
PharmGKBiPA162401519.

Polymorphism and mutation databases

BioMutaiRNF115.
DMDMi56405389.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000563142 – 304E3 ubiquitin-protein ligase RNF115Add BLAST303

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Post-translational modificationi

RING-type zinc finger-dependent and E2-dependent autoubiquitination.2 Publications

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

EPDiQ9Y4L5.
MaxQBiQ9Y4L5.
PaxDbiQ9Y4L5.
PeptideAtlasiQ9Y4L5.
PRIDEiQ9Y4L5.

PTM databases

iPTMnetiQ9Y4L5.
PhosphoSitePlusiQ9Y4L5.

Expressioni

Tissue specificityi

Expressed at extremely low levels in normal breast, prostate, lung, colon. Higher levels of expression are detected in heart, skeletal muscle, testis as well as in breast and prostate cancer cells.1 Publication

Gene expression databases

BgeeiENSG00000265491.
CleanExiHS_RNF115.
GenevisibleiQ9Y4L5. HS.

Organism-specific databases

HPAiHPA019130.

Interactioni

Subunit structurei

Interacts with RAB7A. Interacts with EGFR and FLT3.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi118094. 49 interactors.
IntActiQ9Y4L5. 10 interactors.
STRINGi9606.ENSP00000358297.

Structurei

3D structure databases

ProteinModelPortaliQ9Y4L5.
SMRiQ9Y4L5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri228 – 269RING-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0800. Eukaryota.
ENOG41121N2. LUCA.
GeneTreeiENSGT00530000062967.
HOGENOMiHOG000116417.
HOVERGENiHBG059832.
InParanoidiQ9Y4L5.
KOiK11982.
OMAiCKGEVNP.
OrthoDBiEOG091G0SOO.
PhylomeDBiQ9Y4L5.
TreeFamiTF317985.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
PfamiView protein in Pfam
PF13639. zf-RING_2. 1 hit.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
PROSITEiView protein in PROSITE
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Y4L5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEASAAGAD SGAAVAAHRF FCHFCKGEVS PKLPEYICPR CESGFIEEVT
60 70 80 90 100
DDSSFLGGGG SRIDNTTTTH FAELWGHLDH TMFFQDFRPF LSSSPLDQDN
110 120 130 140 150
RANERGHQTH TDFWGARPPR LPLGRRYRSR GSSRPDRSPA IEGILQHIFA
160 170 180 190 200
GFFANSAIPG SPHPFSWSGM LHSNPGDYAW GQTGLDAIVT QLLGQLENTG
210 220 230 240 250
PPPADKEKIT SLPTVTVTQE QVDMGLECPV CKEDYTVEEE VRQLPCNHFF
260 270 280 290 300
HSSCIVPWLE LHDTCPVCRK SLNGEDSTRQ SQSTEASASN RFSNDSQLHD

RWTF
Length:304
Mass (Da):33,703
Last modified:December 7, 2004 - v2
Checksum:i22F0C8FCC0F55045
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti299H → N in CAB45280 (Ref. 7) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052105194G → R. Corresponds to variant dbSNP:rs11577731Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF542552 mRNA. Translation: AAQ09535.1.
AF419857 mRNA. Translation: AAP97292.1.
AK290749 mRNA. Translation: BAF83438.1.
AL390725, AL160282 Genomic DNA. Translation: CAI13717.1.
CH471244 Genomic DNA. Translation: EAW71437.1.
BC054049 mRNA. Translation: AAH54049.1.
BC064903 mRNA. Translation: AAH64903.1.
AL079314 mRNA. Translation: CAB45280.1.
CCDSiCCDS72863.1.
RefSeqiNP_055270.1. NM_014455.3.
UniGeneiHs.523550.

Genome annotation databases

EnsembliENST00000582693; ENSP00000463650; ENSG00000265491.
GeneIDi27246.
KEGGihsa:27246.
UCSCiuc031utf.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiRN115_HUMAN
AccessioniPrimary (citable) accession number: Q9Y4L5
Secondary accession number(s): A8K3Y4, Q5T2V9, Q7Z2J2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: December 7, 2004
Last modified: August 30, 2017
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways