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Q9Y4L5 (RN115_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
E3 ubiquitin-protein ligase RNF115

EC=6.3.2.-
Alternative name(s):
RING finger protein 115
Rabring 7
Zinc finger protein 364
Gene names
Name:RNF115
Synonyms:ZNF364
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length304 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as an E2-dependent E3 ubiquitin-protein ligase. May be involved in endocytic trafficking. Ref.8 Ref.10

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with RAB7A. Ref.9

Subcellular location

Cytoplasmcytosol By similarity. Note: The GTP-bound form of RAB7A recruits RNF115 from the cytosol onto late endosomes/lysosomes By similarity.

Tissue specificity

Expressed at extremely low levels in normal breast, prostate, lung, colon. Higher levels of expression are detected in heart, skeletal muscle, testis as well as in breast and prostate cancer cells. Ref.8

Post-translational modification

RING-type zinc finger-dependent and E2-dependent autoubiquitination.

Sequence similarities

Contains 1 RING-type zinc finger.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentCytoplasm
   Coding sequence diversityPolymorphism
   DomainZinc-finger
   LigandMetal-binding
Zinc
   Molecular functionLigase
   PTMAcetylation
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein autoubiquitination

Inferred from direct assay Ref.8. Source: UniProtKB

   Cellular_componentcytosol

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionubiquitin-protein ligase activity

Inferred from direct assay Ref.10. Source: UniProtKB

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.11
Chain2 – 304303E3 ubiquitin-protein ligase RNF115
PRO_0000056314

Regions

Zinc finger228 – 26942RING-type

Amino acid modifications

Modified residue21N-acetylalanine Ref.11

Natural variations

Natural variant1941G → R.
Corresponds to variant rs11577731 [ dbSNP | Ensembl ].
VAR_052105

Experimental info

Mutagenesis261K → R: Loss of autoubiquitination; when associated with R-32. Ref.10
Mutagenesis321K → R: Loss of autoubiquitination; when associated with R-26. Ref.10
Mutagenesis2281C → A: Loss of autoubiquitination. Ref.10
Mutagenesis2311C → A: Loss of autoubiquitination. Ref.10
Sequence conflict2991H → N in CAB45280. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q9Y4L5 [UniParc].

Last modified December 7, 2004. Version 2.
Checksum: 22F0C8FCC0F55045

FASTA30433,703
        10         20         30         40         50         60 
MAEASAAGAD SGAAVAAHRF FCHFCKGEVS PKLPEYICPR CESGFIEEVT DDSSFLGGGG 

        70         80         90        100        110        120 
SRIDNTTTTH FAELWGHLDH TMFFQDFRPF LSSSPLDQDN RANERGHQTH TDFWGARPPR 

       130        140        150        160        170        180 
LPLGRRYRSR GSSRPDRSPA IEGILQHIFA GFFANSAIPG SPHPFSWSGM LHSNPGDYAW 

       190        200        210        220        230        240 
GQTGLDAIVT QLLGQLENTG PPPADKEKIT SLPTVTVTQE QVDMGLECPV CKEDYTVEEE 

       250        260        270        280        290        300 
VRQLPCNHFF HSSCIVPWLE LHDTCPVCRK SLNGEDSTRQ SQSTEASASN RFSNDSQLHD 


RWTF 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of zinc finger protein 364, C3HC4 type (ZNF364), a novel human member of zinc finger protein family."
Zhang D.L., Cai J.J., Ma D.L.
Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Guo J.H., She X.Y., Yu L.
Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pancreas and Skin.
[7]The European IMAGE consortium
Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 73-304.
Tissue: Uterus.
[8]"A novel RING-type ubiquitin ligase breast cancer-associated gene 2 correlates with outcome in invasive breast cancer."
Burger A.M., Gao Y., Amemiya Y., Kahn H.J., Kitching R., Yang Y., Sun P., Narod S.A., Hanna W.M., Seth A.K.
Cancer Res. 65:10401-10412(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, AUTOUBIQUITINATION, TISSUE SPECIFICITY.
[9]"Novel RING E3 ubiquitin ligases in breast cancer."
Burger A., Amemiya Y., Kitching R., Seth A.K.
Neoplasia 8:689-695(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RAB7A.
[10]"Autoubiquitination of BCA2 RING E3 ligase regulates its own stability and affects cell migration."
Amemiya Y., Azmi P., Seth A.
Mol. Cancer Res. 6:1385-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, AUTOUBIQUITINATION, MUTAGENESIS OF LYS-26; LYS-32; CYS-228 AND CYS-231.
[11]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF542552 mRNA. Translation: AAQ09535.1.
AF419857 mRNA. Translation: AAP97292.1.
AK290749 mRNA. Translation: BAF83438.1.
AL390725, AL160282 Genomic DNA. Translation: CAI13717.1.
CH471244 Genomic DNA. Translation: EAW71437.1.
BC054049 mRNA. Translation: AAH54049.1.
BC064903 mRNA. Translation: AAH64903.1.
AL079314 mRNA. Translation: CAB45280.1.
RefSeqNP_055270.1. NM_014455.3.
UniGeneHs.523550.

3D structure databases

ProteinModelPortalQ9Y4L5.
SMRQ9Y4L5. Positions 218-277.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid118094. 32 interactions.
IntActQ9Y4L5. 8 interactions.
STRING9606.ENSP00000358297.

PTM databases

PhosphoSiteQ9Y4L5.

Polymorphism databases

DMDM56405389.

Proteomic databases

PaxDbQ9Y4L5.
PRIDEQ9Y4L5.

Protocols and materials databases

DNASU27246.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000369291; ENSP00000358297; ENSG00000121848.
GeneID27246.
KEGGhsa:27246.
UCSCuc001eoj.3. human.

Organism-specific databases

CTD27246.
GeneCardsGC01P145611.
HGNCHGNC:18154. RNF115.
HPAHPA019130.
neXtProtNX_Q9Y4L5.
PharmGKBPA162401519.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG235630.
HOGENOMHOG000116417.
HOVERGENHBG059832.
InParanoidQ9Y4L5.
KOK11982.
OMASTHFAEF.
OrthoDBEOG7353XB.
PhylomeDBQ9Y4L5.
TreeFamTF317985.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ9Y4L5.
BgeeQ9Y4L5.
CleanExHS_RNF115.
GenevestigatorQ9Y4L5.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF13639. zf-RING_2. 1 hit.
[Graphical view]
SMARTSM00184. RING. 1 hit.
[Graphical view]
PROSITEPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRNF115. human.
GeneWikiZNF364.
GenomeRNAi27246.
NextBio50157.
PROQ9Y4L5.

Entry information

Entry nameRN115_HUMAN
AccessionPrimary (citable) accession number: Q9Y4L5
Secondary accession number(s): A8K3Y4, Q5T2V9, Q7Z2J2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: December 7, 2004
Last modified: April 16, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM