Q9Y4I5 (MTL5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tesmin

Alternative name(s):
Metallothionein-like 5, testis-specific
Testis-specific metallothionein-like protein

Gene names

Name:

MTL5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May have a role in spermatogenesis Probable.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Tissue specificity	Expressed specifically in testis.
Sequence similarities	Belongs to the lin-54 family. Contains 1 CRC domain.
Sequence caution	The sequence AAD24668.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Biological process	Differentiation Spermatogenesis
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Developmental protein
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW cellular metal ion homeostasis Non-traceable author statement Ref.4. Source: UniProtKB multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW response to metal ion Non-traceable author statement Ref.4. Source: UniProtKB spermatogenesis Non-traceable author statement Ref.4. Source: UniProtKB
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	metal ion binding Non-traceable author statement Ref.4. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y4I5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y4I5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     307-508: Missing.

Isoform 3 (identifier: Q9Y4I5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     251-251: D → A
     252-508: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 508	508	Tesmin		PRO_0000096626
Regions
	Domain	292 – 405	114	CRC
Amino acid modifications
	Modified residue	40	1	N6-acetyllysine Ref.5
Natural variations
	Alternative sequence	251	1	D → A in isoform 3.		VSP_035297
	Alternative sequence	252 – 508	257	Missing in isoform 3.		VSP_035298
	Alternative sequence	307 – 508	202	Missing in isoform 2.		VSP_035299
	Natural variant	223	1	C → R. Ref.1 Corresponds to variant rs12365708 [ dbSNP | Ensembl ].		VAR_054067
	Natural variant	260	1	V → A. Corresponds to variant rs35773957 [ dbSNP | Ensembl ].		VAR_062236
Experimental info
	Sequence conflict	191	1	S → W in AAD24668. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: 44DA371A81E012C0	FASTA	508	55,025
	10 20 30 40 50 60 MEEGPLPGGL PSPEDAMVTE LLSPEGPFAS ENIGLKAPVK YEEDEFHVFK EAYLGPADPK 70 80 90 100 110 120 EPVLHAFNPA LGADCKGQVK AKLAGGDSDG GELLGEYPGI PELSALEDVA LLQAPQPPAC 130 140 150 160 170 180 NVHFLSSLLP AHRSPAVLPL GAWVLEGASH PGVRMIPVEI KEAGGTTTSN NPEEATLQNL 190 200 210 220 230 240 LAQESCCKFP SSQELEDASC CSLKKDSNPM VICQLKGGTQ MLCIDNSRTR ELKALHLVPQ 250 260 270 280 290 300 YQDQNNYLQS DVPKPMTALV GRFLPASTKL NLITQQLEGA LPSVVNGSAF PSGSTLPGPP 310 320 330 340 350 360 KITLAGYCDC FASGDFCNNC NCNNCCNNLH HDIERFKAIK ACLGRNPEAF QPKIGKGQLG 370 380 390 400 410 420 NVKPQHNKGC NCRRSGCLKN YCECYEAQIM CSSICKCIGC KNYEESPERK TLMSMPNYMQ 430 440 450 460 470 480 TGGLEGSHYL PPTKFSGLPR FSHDRRPSSC ISWEVVEATC ACLLAQGEEA EKEHCSKCLA 490 500 EQMILEEFGR CLSQILHTEF KSKGLKME
	Isoform 2 [UniParc]. Checksum: D9418436E083840A	FASTA	306	32,305
	10 20 30 40 50 60 MEEGPLPGGL PSPEDAMVTE LLSPEGPFAS ENIGLKAPVK YEEDEFHVFK EAYLGPADPK 70 80 90 100 110 120 EPVLHAFNPA LGADCKGQVK AKLAGGDSDG GELLGEYPGI PELSALEDVA LLQAPQPPAC 130 140 150 160 170 180 NVHFLSSLLP AHRSPAVLPL GAWVLEGASH PGVRMIPVEI KEAGGTTTSN NPEEATLQNL 190 200 210 220 230 240 LAQESCCKFP SSQELEDASC CSLKKDSNPM VICQLKGGTQ MLCIDNSRTR ELKALHLVPQ 250 260 270 280 290 300 YQDQNNYLQS DVPKPMTALV GRFLPASTKL NLITQQLEGA LPSVVNGSAF PSGSTLPGPP KITLAG
	Isoform 3 [UniParc]. Checksum: 4D37EB2616DD1106	FASTA	251	26,737
	10 20 30 40 50 60 MEEGPLPGGL PSPEDAMVTE LLSPEGPFAS ENIGLKAPVK YEEDEFHVFK EAYLGPADPK 70 80 90 100 110 120 EPVLHAFNPA LGADCKGQVK AKLAGGDSDG GELLGEYPGI PELSALEDVA LLQAPQPPAC 130 140 150 160 170 180 NVHFLSSLLP AHRSPAVLPL GAWVLEGASH PGVRMIPVEI KEAGGTTTSN NPEEATLQNL 190 200 210 220 230 240 LAQESCCKFP SSQELEDASC CSLKKDSNPM VICQLKGGTQ MLCIDNSRTR ELKALHLVPQ 250 YQDQNNYLQS A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), VARIANT ARG-223. Tissue: Testis.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Testis.
[4]	"A novel testis-specific metallothionein-like protein, tesmin, is an early marker of male germ cell differentiation." Sugihara T., Wadhwa R., Kaul S.C., Mitsui Y. Genomics 57:130-136(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 79-508 (ISOFORM 1). Tissue: Testis.
[5]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-40, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK074817 mRNA. Translation: BAC11228.1. AK292358 mRNA. Translation: BAF85047.1. CH471076 Genomic DNA. Translation: EAW74719.1. BC027593 mRNA. Translation: AAH27593.1. BC064579 mRNA. Translation: AAH64579.1. U86074 mRNA. Translation: AAD24668.1. Frameshift.
IPI	IPI00007395. IPI00738914. IPI00903174.
RefSeq	NP_001034745.1. NM_001039656.1. NP_004914.2. NM_004923.3.
UniGene	Hs.145932.
3D structure databases
ProteinModelPortal	Q9Y4I5.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000255087.
PTM databases
PhosphoSite	Q9Y4I5.
Polymorphism databases
DMDM	205371773.
Proteomic databases
PaxDb	Q9Y4I5.
PRIDE	Q9Y4I5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000255087; ENSP00000255087; ENSG00000132749. ENST00000443940; ENSP00000403086; ENSG00000132749. ENST00000544963; ENSP00000440968; ENSG00000132749.
GeneID	9633.
KEGG	hsa:9633.
UCSC	uc001ooc.3. human. uc009ysi.1. human.
Organism-specific databases
CTD	9633.
GeneCards	GC11M068474.
H-InvDB	HIX0026150.
HGNC	HGNC:7446. MTL5.
HPA	HPA018539.
MIM	604374. gene.
neXtProt	NX_Q9Y4I5.
PharmGKB	PA31249.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG303998.
HOGENOM	HOG000082453.
HOVERGEN	HBG108153.
InParanoid	Q9Y4I5.
OMA	ILHTEFK.
OrthoDB	EOG4ZKJM3.
PhylomeDB	Q9Y4I5.
Gene expression databases
Bgee	Q9Y4I5.
CleanEx	HS_MTL5.
Genevestigator	Q9Y4I5.
GermOnline	ENSG00000132749. Homo sapiens.
Family and domain databases
InterPro	IPR005172. CRC. [Graphical view]
Pfam	PF03638. CXC. 2 hits. [Graphical view]
PROSITE	PS51634. CRC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	9633.
NextBio	36155.
SOURCE	Search...

Entry information

Entry name

MTL5_HUMAN

Accession

Primary (citable) accession number: Q9Y4I5
Secondary accession number(s): A8K8J3 , Q4G182, Q6P2E2, Q8NCC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	September 2, 2008
Last modified:	April 3, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.