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Protein

G-protein-signaling modulator 3

Gene

GPSM3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with subunit of G(i) alpha proteins and regulates the activation of G(i) alpha proteins.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein-signaling modulator 3
Alternative name(s):
Activator of G-protein signaling 4
G18.1b
Protein G18
Gene namesi
Name:GPSM3
Synonyms:AGS4, C6orf9, G18
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componentsi: Chromosome 6, Unplaced

Organism-specific databases

HGNCiHGNC:13945. GPSM3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi121 – 1211A → D: Restores G(i) alpha binding and GDI activity of the GoLoco 2 domain. 1 Publication

Organism-specific databases

PharmGKBiPA25940.

Polymorphism and mutation databases

BioMutaiGPSM3.
DMDMi74753502.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 160160G-protein-signaling modulator 3PRO_0000233717Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei35 – 351Phosphoserine1 Publication
Modified residuei39 – 391Phosphoserine1 Publication
Modified residuei56 – 561PhosphoserineBy similarity
Modified residuei59 – 591Phosphoserine1 Publication
Modified residuei62 – 621Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Y4H4.
PaxDbiQ9Y4H4.
PRIDEiQ9Y4H4.

PTM databases

PhosphoSiteiQ9Y4H4.

Expressioni

Tissue specificityi

Expressed in heart, placenta, lung and liver.1 Publication

Gene expression databases

BgeeiQ9Y4H4.
CleanExiHS_GPSM3.
ExpressionAtlasiQ9Y4H4. baseline and differential.
GenevestigatoriQ9Y4H4.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
GNAI1P630963EBI-347538,EBI-618639
GNAI2P048993EBI-347538,EBI-353997
GNAI3P087543EBI-347538,EBI-357563
MEOX2A4D1273EBI-347538,EBI-10172134
TADA2AO754783EBI-347538,EBI-742268
USHBP1Q8N6Y03EBI-347538,EBI-739895

Protein-protein interaction databases

BioGridi122005. 7 interactions.
IntActiQ9Y4H4. 7 interactions.
MINTiMINT-1034681.
STRINGi9606.ENSP00000364180.

Structurei

3D structure databases

ProteinModelPortaliQ9Y4H4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini62 – 8423GoLoco 1PROSITE-ProRule annotationAdd
BLAST
Domaini104 – 12623GoLoco 2PROSITE-ProRule annotationAdd
BLAST
Domaini132 – 15524GoLoco 3PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi16 – 4328Pro-richAdd
BLAST

Domaini

The GoLoco 1 and/or GoLoco 3 domains exhibit GDI activity towards GDP-bound G(i) alpha protein, but not the GoLoco 2 domain.1 Publication

Sequence similaritiesi

Contains 3 GoLoco domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG77431.
GeneTreeiENSGT00390000002471.
HOGENOMiHOG000232123.
HOVERGENiHBG052882.
InParanoidiQ9Y4H4.
KOiK15838.
OMAiCQRIEAQ.
PhylomeDBiQ9Y4H4.
TreeFamiTF339136.

Family and domain databases

InterProiIPR024800. G_prot_signal_mod_3.
IPR003109. GoLoco_motif.
[Graphical view]
PANTHERiPTHR10098:SF99. PTHR10098:SF99. 1 hit.
PfamiPF02188. GoLoco. 3 hits.
[Graphical view]
SMARTiSM00390. GoLoco. 3 hits.
[Graphical view]
PROSITEiPS50877. GOLOCO. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Y4H4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAERPQEEE DGEQGPPQDE EGWPPPNSTT RPWRSAPPSP PPPGTRHTAL
60 70 80 90 100
GPRSASLLSL QTELLLDLVA EAQSRRLEEQ RATFYTPQNP SSLAPAPLRP
110 120 130 140 150
LEDREQLYST ILSHQCQRME AQRSEPPLPP GGQELLELLL RVQGGGRMEE
160
QRSRPPTHTC
Length:160
Mass (Da):17,866
Last modified:November 1, 1999 - v1
Checksum:i42B3D207D2C48428
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 141Q → R (PubMed:15096500).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243937 mRNA. Translation: CAB51288.1.
AF155657 mRNA. Translation: AAF67476.1.
AK313922 mRNA. Translation: BAG36643.1.
AL662830 Genomic DNA. Translation: CAI17541.1.
AL662884 Genomic DNA. Translation: CAI18360.1.
AL845464 Genomic DNA. Translation: CAI41816.1.
BX284686 Genomic DNA. Translation: CAM26229.1.
BX927239 Genomic DNA. Translation: CAQ06601.1.
CR933878 Genomic DNA. Translation: CAQ09630.1.
CR812478 Genomic DNA. Translation: CAQ10705.1.
CH471081 Genomic DNA. Translation: EAX03620.1.
BC018724 mRNA. Translation: AAH18724.1.
CCDSiCCDS34419.1.
RefSeqiNP_001263430.1. NM_001276501.1.
NP_071390.1. NM_022107.1.
UniGeneiHs.520046.

Genome annotation databases

EnsembliENST00000375040; ENSP00000364180; ENSG00000213654.
ENST00000375043; ENSP00000364183; ENSG00000213654.
ENST00000383265; ENSP00000372752; ENSG00000206314.
ENST00000383269; ENSP00000372756; ENSG00000206314.
ENST00000414839; ENSP00000405026; ENSG00000236697.
ENST00000420041; ENSP00000413975; ENSG00000233490.
ENST00000424520; ENSP00000413430; ENSG00000237052.
ENST00000429209; ENSP00000392487; ENSG00000234243.
ENST00000432871; ENSP00000414939; ENSG00000234508.
ENST00000441705; ENSP00000396786; ENSG00000234243.
ENST00000445326; ENSP00000407538; ENSG00000234508.
ENST00000448684; ENSP00000403132; ENSG00000236697.
ENST00000453667; ENSP00000414024; ENSG00000233490.
ENST00000457070; ENSP00000410087; ENSG00000237052.
GeneIDi63940.
KEGGihsa:63940.
UCSCiuc003oay.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243937 mRNA. Translation: CAB51288.1.
AF155657 mRNA. Translation: AAF67476.1.
AK313922 mRNA. Translation: BAG36643.1.
AL662830 Genomic DNA. Translation: CAI17541.1.
AL662884 Genomic DNA. Translation: CAI18360.1.
AL845464 Genomic DNA. Translation: CAI41816.1.
BX284686 Genomic DNA. Translation: CAM26229.1.
BX927239 Genomic DNA. Translation: CAQ06601.1.
CR933878 Genomic DNA. Translation: CAQ09630.1.
CR812478 Genomic DNA. Translation: CAQ10705.1.
CH471081 Genomic DNA. Translation: EAX03620.1.
BC018724 mRNA. Translation: AAH18724.1.
CCDSiCCDS34419.1.
RefSeqiNP_001263430.1. NM_001276501.1.
NP_071390.1. NM_022107.1.
UniGeneiHs.520046.

3D structure databases

ProteinModelPortaliQ9Y4H4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122005. 7 interactions.
IntActiQ9Y4H4. 7 interactions.
MINTiMINT-1034681.
STRINGi9606.ENSP00000364180.

PTM databases

PhosphoSiteiQ9Y4H4.

Polymorphism and mutation databases

BioMutaiGPSM3.
DMDMi74753502.

Proteomic databases

MaxQBiQ9Y4H4.
PaxDbiQ9Y4H4.
PRIDEiQ9Y4H4.

Protocols and materials databases

DNASUi63940.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000375040; ENSP00000364180; ENSG00000213654.
ENST00000375043; ENSP00000364183; ENSG00000213654.
ENST00000383265; ENSP00000372752; ENSG00000206314.
ENST00000383269; ENSP00000372756; ENSG00000206314.
ENST00000414839; ENSP00000405026; ENSG00000236697.
ENST00000420041; ENSP00000413975; ENSG00000233490.
ENST00000424520; ENSP00000413430; ENSG00000237052.
ENST00000429209; ENSP00000392487; ENSG00000234243.
ENST00000432871; ENSP00000414939; ENSG00000234508.
ENST00000441705; ENSP00000396786; ENSG00000234243.
ENST00000445326; ENSP00000407538; ENSG00000234508.
ENST00000448684; ENSP00000403132; ENSG00000236697.
ENST00000453667; ENSP00000414024; ENSG00000233490.
ENST00000457070; ENSP00000410087; ENSG00000237052.
GeneIDi63940.
KEGGihsa:63940.
UCSCiuc003oay.4. human.

Organism-specific databases

CTDi63940.
GeneCardsiGC06M032165.
GC06Mi32169.
GC06Mj32107.
GC06Ml32197.
GC06Mm32234.
GC06Mn32116.
GC06Mo32165.
HGNCiHGNC:13945. GPSM3.
neXtProtiNX_Q9Y4H4.
PharmGKBiPA25940.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG77431.
GeneTreeiENSGT00390000002471.
HOGENOMiHOG000232123.
HOVERGENiHBG052882.
InParanoidiQ9Y4H4.
KOiK15838.
OMAiCQRIEAQ.
PhylomeDBiQ9Y4H4.
TreeFamiTF339136.

Miscellaneous databases

GenomeRNAii63940.
NextBioi65706.
PROiQ9Y4H4.

Gene expression databases

BgeeiQ9Y4H4.
CleanExiHS_GPSM3.
ExpressionAtlasiQ9Y4H4. baseline and differential.
GenevestigatoriQ9Y4H4.

Family and domain databases

InterProiIPR024800. G_prot_signal_mod_3.
IPR003109. GoLoco_motif.
[Graphical view]
PANTHERiPTHR10098:SF99. PTHR10098:SF99. 1 hit.
PfamiPF02188. GoLoco. 3 hits.
[Graphical view]
SMARTiSM00390. GoLoco. 3 hits.
[Graphical view]
PROSITEiPS50877. GOLOCO. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of AGS4: a protein containing three G-protein regulatory motifs that regulate the activation state of Gialpha."
    Cao X., Cismowski M.J., Sato M., Blumer J.B., Lanier S.M.
    J. Biol. Chem. 279:27567-27574(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Lung.
  2. "Characterisation of the novel gene G18, located in the class III region of the human major histocompatibility complex."
    Kendall E., Campbell R.D.
    Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "A novel gene expressed in human adrenal gland."
    Li Y., Fu S., Huang C., Jiang C., Ren S., Zhou J., Yu Y., Xu S., Wang Y., Fu G., Chen Z., Han Z.
    Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Adrenal gland.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Umbilical cord blood.
  5. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: B-cell.
  8. "Guanine nucleotide dissociation inhibitor activity of the triple GoLoco motif protein G18: alanine-to-aspartate mutation restores function to an inactive second GoLoco motif."
    Kimple R.J., Willard F.S., Hains M.D., Jones M.B., Nweke G.K., Siderovski D.P.
    Biochem. J. 378:801-808(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ALA-121, DOMAIN.
  9. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  10. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-35; SER-39; SER-59 AND THR-62, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiGPSM3_HUMAN
AccessioniPrimary (citable) accession number: Q9Y4H4
Secondary accession number(s): A2BFJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: November 1, 1999
Last modified: April 29, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.