Q9Y4G6 (TLN2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Talin-2

Gene names

Name:	TLN2
Synonyms:	KIAA0320

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	2542 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	As a major component of focal adhesion plaques that links integrin to the actin cytoskeleton, may play an important role in cell adhesion. Recruits PIP5K1C to focal adhesion plaques and strongly activates its kinase activity By similarity.
Subunit structure	Interacts directly with PIP5K1C.
Subcellular location	Cell junction › focal adhesion. Cell junction › synapse. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm › cytoskeleton. Note: Focal adhesion plaques and synapses.
Sequence similarities	Contains 1 FERM domain. Contains 1 I/LWEQ domain.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Cytoplasm Cytoskeleton Membrane Synapse
Coding sequence diversity	Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Non-traceable author statement PubMed 11907066. Source: UniProtKB cell-cell junction assembly Traceable author statement PubMed 15494027. Source: UniProtKB cytoskeletal anchoring at plasma membrane Inferred from electronic annotation. Source: InterPro
Cellular_component	actin cytoskeleton Non-traceable author statement PubMed 10320934. Source: UniProtKB cell-cell junction Inferred by curator PubMed 15494027. Source: UniProtKB cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW fascia adherens Inferred from electronic annotation. Source: Compara focal adhesion Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell ruffle Inferred from electronic annotation. Source: InterPro synapse Non-traceable author statement PubMed 11907066. Source: UniProtKB
Molecular_function	actin binding Non-traceable author statement PubMed 10320934. Source: UniProtKB structural constituent of cytoskeleton Inferred from electronic annotation. Source: InterPro structural molecule activity Non-traceable author statement PubMed 10320934. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2542	2542	Talin-2		PRO_0000219431
Regions
	Domain	88 – 406	319	FERM
	Domain	2294 – 2533	240	I/LWEQ
	Region	312 – 406	95	Interaction with PIP5K1C By similarity
	Compositional bias	861 – 1193	333	Ala-rich
Amino acid modifications
	Modified residue	28	1	Phosphotyrosine By similarity
	Modified residue	1665	1	Phosphotyrosine By similarity
	Modified residue	1843	1	Phosphothreonine Ref.6 Ref.9
Natural variations
	Natural variant	340	1	V → A. Corresponds to variant rs11634784 [ dbSNP | Ensembl ].		VAR_055313
	Natural variant	1148	1	A → S. Corresponds to variant rs2280279 [ dbSNP | Ensembl ].		VAR_014432
	Natural variant	1148	1	A → T. Corresponds to variant rs2280279 [ dbSNP | Ensembl ].		VAR_059136
	Natural variant	1877	1	V → I. Corresponds to variant rs7182971 [ dbSNP | Ensembl ].		VAR_055314
	Natural variant	2144	1	T → I. Corresponds to variant rs11633796 [ dbSNP | Ensembl ].		VAR_055315
	Natural variant	2266	1	F → L. Corresponds to variant rs3816988 [ dbSNP | Ensembl ].		VAR_014433
Experimental info
	Sequence conflict	285	1	E → A in AAM73764. Ref.1
	Sequence conflict	1269	1	L → F in AAM73764. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9Y4G6 [UniParc]. Last modified May 5, 2009. Version 4. Checksum: 802D50915F4FD4B0	FASTA	2,542	271,613
	10 20 30 40 50 60 MVALSLKICV RHCNVVKTMQ FEPSTAVYDA CRVIRERVPE AQTGQASDYG LFLSDEDPRK 70 80 90 100 110 120 GIWLEAGRTL DYYMLRNGDI LEYKKKQRPQ KIRMLDGSVK TVMVDDSKTV GELLVTICSR 130 140 150 160 170 180 IGITNYEEYS LIQETIEEKK EEGTGTLKKD RTLLRDERKM EKLKAKLHTD DDLNWLDHSR 190 200 210 220 230 240 TFREQGVDEN ETLLLRRKFF YSDQNVDSRD PVQLNLLYVQ ARDDILNGSH PVSFEKACEF 250 260 270 280 290 300 GGFQAQIQFG PHVEHKHKPG FLDLKEFLPK EYIKQRGAEK RIFQEHKNCG EMSEIEAKVK 310 320 330 340 350 360 YVKLARSLRT YGVSFFLVKE KMKGKNKLVP RLLGITKDSV MRVDEKTKEV LQEWPLTTVK 370 380 390 400 410 420 RWAASPKSFT LDFGEYQESY YSVQTTEGEQ ISQLIAGYID IILKKKQSKD RFGLEGDEES 430 440 450 460 470 480 TMLEESVSPK KSTILQQQFN RTGKAEHGSV ALPAVMRSGS SGPETFNVGS MPSPQQQVMV 490 500 510 520 530 540 GQMHRGHMPP LTSAQQALMG TINTSMHAVQ QAQDDLSELD SLPPLGQDMA SRVWVQNKVD 550 560 570 580 590 600 ESKHEIHSQV DAITAGTASV VNLTAGDPAD TDYTAVGCAI TTISSNLTEM SKGVKLLAAL 610 620 630 640 650 660 MDDEVGSGED LLRAARTLAG AVSDLLKAVQ PTSGEPRQTV LTAAGSIGQA SGDLLRQIGE 670 680 690 700 710 720 NETDERFQDV LMSLAKAVAN AAAMLVLKAK NVAQVAEDTV LQNRVIAAAT QCALSTSQLV 730 740 750 760 770 780 ACAKVVSPTI SSPVCQEQLI EAGKLVDRSV ENCVRACQAA TTDSELLKQV SAAASVVSQA 790 800 810 820 830 840 LHDLLQHVRQ FASRGEPIGR YDQATDTIMC VTESIFSSMG DAGEMVRQAR VLAQATSDLV 850 860 870 880 890 900 NAMRSDAEAE IDMENSKKLL AAAKLLADST ARMVEAAKGA AANPENEDQQ QRLREAAEGL 910 920 930 940 950 960 RVATNAAAQN AIKKKIVNRL EVAAKQAAAA ATQTIAASQN AAVSNKNPAA QQQLVQSCKA 970 980 990 1000 1010 1020 VADHIPQLVQ GVRGSQAQAE DLSAQLALII SSQNFLQPGS KMVSSAKAAV PTVSDQAAAM 1030 1040 1050 1060 1070 1080 QLSQCAKNLA TSLAELRTAS QKAHEACGPM EIDSALNTVQ TLKNELQDAK MAAVESQLKP 1090 1100 1110 1120 1130 1140 LPGETLEKCA QDLGSTSKAV GSSMAQLLTC AAQGNEHYTG VAARETAQAL KTLAQAARGV 1150 1160 1170 1180 1190 1200 AASTTDPAAA HAMLDSARDV MEGSAMLIQE AKQALIAPGD AERQQRLAQV AKAVSHSLNN 1210 1220 1230 1240 1250 1260 CVNCLPGQKD VDVALKSIGE SSKKLLVDSL PPSTKPFQEA QSELNQAAAD LNQSAGEVVH 1270 1280 1290 1300 1310 1320 ATRGQSGELA AASGKFSDDF DEFLDAGIEM AGQAQTKEDQ IQVIGNLKNI SMASSKLLLA 1330 1340 1350 1360 1370 1380 AKSLSVDPGA PNAKNLLAAA ARAVTESINQ LITLCTQQAP GQKECDNALR ELETVKGMLD 1390 1400 1410 1420 1430 1440 NPNEPVSDLS YFDCIESVME NSKVLGESMA GISQNAKTGD LPAFGECVGI ASKALCGLTE 1450 1460 1470 1480 1490 1500 AAAQAAYLVG ISDPNSQAGH QGLVDPIQFA RANQAIQMAC QNLVDPGSSP SQVLSAATIV 1510 1520 1530 1540 1550 1560 AKHTSALCNA CRIASSKTAN PVAKRHFVQS AKEVANSTAN LVKTIKALDG DFSEDNRNKC 1570 1580 1590 1600 1610 1620 RIATAPLIEA VENLTAFASN PEFVSIPAQI SSEGSQAQEP ILVSAKTMLE SSSYLIRTAR 1630 1640 1650 1660 1670 1680 SLAINPKDPP TWSVLAGHSH TVSDSIKSLI TSIRDKAPGQ RECDYSIDGI NRCIRDIEQA 1690 1700 1710 1720 1730 1740 SLAAVSQSLA TRDDISVEAL QEQLTSVVQE IGHLIDPIAT AARGEAAQLG HKVTQLASYF 1750 1760 1770 1780 1790 1800 EPLILAAVGV ASKILDHQQQ MTVLDQTKTL AESALQMLYA AKEGGGNPKA QHTHDAITEA 1810 1820 1830 1840 1850 1860 AQLMKEAVDD IMVTLNEAAS EVGLVGGMVD AIAEAMSKLD EGTPPEPKGT FVDYQTTVVK 1870 1880 1890 1900 1910 1920 YSKAIAVTAQ EMMTKSVTNP EELGGLASQM TSDYGHLAFQ GQMAAATAEP EEIGFQIRTR 1930 1940 1950 1960 1970 1980 VQDLGHGCIF LVQKAGALQV CPTDSYTKRE LIECARAVTE KVSLVLSALQ AGNKGTQACI 1990 2000 2010 2020 2030 2040 TAATAVSGII ADLDTTIMFA TAGTLNAENS ETFADHRENI LKTAKALVED TKLLVSGAAS 2050 2060 2070 2080 2090 2100 TPDKLAQAAQ SSAATITQLA EVVKLGAASL GSDDPETQVV LINAIKDVAK ALSDLISATK 2110 2120 2130 2140 2150 2160 GAASKPVDDP SMYQLKGAAK VMVTNVTSLL KTVKAVEDEA TRGTRALEAT IECIKQELTV 2170 2180 2190 2200 2210 2220 FQSKDVPEKT SSPEESIRMT KGITMATAKA VAAGNSCRQE DVIATANLSR KAVSDMLTAC 2230 2240 2250 2260 2270 2280 KQASFHPDVS DEVRTRALRF GTECTLGYLD LLEHVLVILQ KPTPEFKQQL AAFSKRVAGA 2290 2300 2310 2320 2330 2340 VTELIQAAEA MKGTEWVDPE DPTVIAETEL LGAAASIEAA AKKLEQLKPR AKPKQADETL 2350 2360 2370 2380 2390 2400 DFEEQILEAA KSIAAATSAL VKSASAAQRE LVAQGKVGSI PANAADDGQW SQGLISAARM 2410 2420 2430 2440 2450 2460 VAAATSSLCE AANASVQGHA SEEKLISSAK QVAASTAQLL VACKVKADQD SEAMRRLQAA 2470 2480 2490 2500 2510 2520 GNAVKRASDN LVRAAQKAAF GKADDDDVVV KTKFVGGIAQ IIAAQEEMLK KERELEEARK 2530 2540 KLAQIRQQQY KFLPTELRED EG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Recruitment and regulation of phosphatidylinositol phosphate kinase type 1 gamma by the FERM domain of talin." Di Paolo G., Pellegrini L., Letinic K., Cestra G., Zoncu R., Voronov S., Chang S., Guo J., Wenk M.R., De Camilli P. Nature 420:85-89(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain and Skeletal muscle.
[2]	"Analysis of the mammalian talin2 gene TLN2." Monkley S.J., Pritchard C.A., Critchley D.R. Biochem. Biophys. Res. Commun. 286:880-885(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Analysis of the DNA sequence and duplication history of human chromosome 15." Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C. Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 4:141-150(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 610-2542. Tissue: Brain.
[5]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[6]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1843, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1843, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF402000 mRNA. Translation: AAM73764.1. AC068233 Genomic DNA. No translation available. AC100839 Genomic DNA. No translation available. AC103740 Genomic DNA. No translation available. AB002318 mRNA. Translation: BAA20778.2.
IPI	IPI00219299.
RefSeq	NP_055874.2. NM_015059.2.
UniGene	Hs.569438. Hs.610975.
3D structure databases
ProteinModelPortal	Q9Y4G6.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-17039N.
IntAct	Q9Y4G6. 3 interactions.
MINT	MINT-5005773.
STRING	9606.ENSP00000303476.
PTM databases
PhosphoSite	Q9Y4G6.
Polymorphism databases
DMDM	229463036.
Proteomic databases
PaxDb	Q9Y4G6.
PRIDE	Q9Y4G6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000306829; ENSP00000303476; ENSG00000171914. ENST00000561311; ENSP00000453508; ENSG00000171914.
GeneID	83660.
KEGG	hsa:83660.
UCSC	uc002alb.4. human.
Organism-specific databases
CTD	83660.
GeneCards	GC15P062682.
H-InvDB	HIX0012315.
HGNC	HGNC:15447. TLN2.
HPA	CAB017194.
MIM	607349. gene.
neXtProt	NX_Q9Y4G6.
PharmGKB	PA37958.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG324465.
HOGENOM	HOG000006734.
HOVERGEN	HBG023870.
InParanoid	Q9Y4G6.
KO	K06271.
OMA	KQASFHP.
OrthoDB	EOG48PMJ8.
Gene expression databases
ArrayExpress	Q9Y4G6.
Bgee	Q9Y4G6.
CleanEx	HS_TLN2.
Genevestigator	Q9Y4G6.
GermOnline	ENSG00000171914. Homo sapiens.
Family and domain databases
Gene3D	1.20.1420.10. 1 hit. 1.20.80.10. 1 hit. 2.30.29.30. 1 hit.
InterPro	IPR019749. Band_41_domain. IPR014352. FERM/acyl-CoA-bd_prot_3-hlx. IPR019748. FERM_central. IPR019747. FERM_CS. IPR000299. FERM_domain. IPR018979. FERM_N. IPR002558. ILWEQ_dom. IPR002404. Insln_rcpt_S1. IPR011993. PH_like_dom. IPR015711. Talin-2. IPR015224. Talin_cent. IPR015009. Vinculin-bd_dom. IPR006077. Vinculin/catenin. [Graphical view]
PANTHER	PTHR19981:SF3. PTHR19981:SF3. 1 hit.
Pfam	PF00373. FERM_M. 1 hit. PF09379. FERM_N. 1 hit. PF01608. I_LWEQ. 1 hit. PF02174. IRS. 1 hit. PF09141. Talin_middle. 1 hit. PF08913. VBS. 1 hit. [Graphical view]
ProDom	PD011820. ILWEQ. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00295. B41. 1 hit. SM00307. ILWEQ. 1 hit. [Graphical view]
SUPFAM	SSF47031. FERM_3-hlx. 1 hit. SSF109880. Talin_cent. 1 hit. SSF47220. Vinculin/catenin. 6 hits.
PROSITE	PS00660. FERM_1. False negative. PS00661. FERM_2. 1 hit. PS50057. FERM_3. 1 hit. PS50945. I_LWEQ. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	83660.
NextBio	72631.
SOURCE	Search...

Entry information

Entry name

TLN2_HUMAN

Accession

Primary (citable) accession number: Q9Y4G6
Secondary accession number(s): A6NLB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	May 5, 2009
Last modified:	May 1, 2013
This is version 128 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.