Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Disheveled-associated activator of morphogenesis 1

Gene

DAAM1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Involved in building functional cilia (PubMed:16630611, PubMed:17482208). Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity).By similarity2 Publications

GO - Biological processi

  • actin cytoskeleton organization Source: InterPro
  • Wnt signaling pathway, planar cell polarity pathway Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiR-HSA-4086400. PCP/CE pathway.
R-HSA-5663220. RHO GTPases Activate Formins.
SignaLinkiQ9Y4D1.
SIGNORiQ9Y4D1.

Names & Taxonomyi

Protein namesi
Recommended name:
Disheveled-associated activator of morphogenesis 1
Gene namesi
Name:DAAM1
Synonyms:KIAA0666
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:18142. DAAM1.

Subcellular locationi

GO - Cellular componenti

  • ciliary basal body Source: UniProtKB
  • cytoplasm Source: HPA
  • cytosol Source: Reactome
  • membrane Source: UniProtKB
  • motile cilium Source: UniProtKB
  • plasma membrane Source: HPA
  • stress fiber Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi698I → A: Abolishes actin-binding. 1 Publication1

Organism-specific databases

DisGeNETi23002.
OpenTargetsiENSG00000100592.
PharmGKBiPA27129.

Polymorphism and mutation databases

BioMutaiDAAM1.
DMDMi34098767.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001949071 – 1078Disheveled-associated activator of morphogenesis 1Add BLAST1078

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34PhosphoserineCombined sources1
Modified residuei1027PhosphoserineCombined sources1
Modified residuei1030PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Y4D1.
PaxDbiQ9Y4D1.
PeptideAtlasiQ9Y4D1.
PRIDEiQ9Y4D1.

PTM databases

iPTMnetiQ9Y4D1.
PhosphoSitePlusiQ9Y4D1.
SwissPalmiQ9Y4D1.

Expressioni

Tissue specificityi

Expressed in all tissues examined.

Gene expression databases

BgeeiENSG00000100592.
CleanExiHS_DAAM1.
ExpressionAtlasiQ9Y4D1. baseline and differential.
GenevisibleiQ9Y4D1. HS.

Organism-specific databases

HPAiHPA026605.

Interactioni

Subunit structurei

Homodimer. Interacts with CIP4, FNBP1 and FNBP1L. Interacts with the SH3 domains of Abl, BTK, endophilin, spectrin and SRC. Binds specifically to GTP-bound CDC42 and RHOA. Interacts with INTU; INTU mediates the indirect interaction between DAAM1 and NPHP4.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-2817289,EBI-2817289
FNBP1LQ5T0N52EBI-2817289,EBI-714058
INTUQ9ULD63EBI-2817289,EBI-11762696
RHOAP615865EBI-2817289,EBI-446668
SRCP005233EBI-2817289,EBI-848039From a different organism.
TRIP10Q15642-22EBI-2817289,EBI-6550597

Protein-protein interaction databases

BioGridi116648. 19 interactors.
DIPiDIP-29641N.
IntActiQ9Y4D1. 23 interactors.
MINTiMINT-1180756.
STRINGi9606.ENSP00000247170.

Structurei

Secondary structure

11078
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi596 – 598Combined sources3
Helixi620 – 622Combined sources3
Beta strandi624 – 626Combined sources3
Helixi627 – 630Combined sources4
Helixi633 – 635Combined sources3
Helixi636 – 639Combined sources4
Helixi642 – 648Combined sources7
Beta strandi650 – 652Combined sources3
Helixi690 – 703Combined sources14
Helixi707 – 715Combined sources9
Helixi725 – 733Combined sources9
Helixi738 – 745Combined sources8
Turni746 – 749Combined sources4
Helixi751 – 753Combined sources3
Helixi756 – 765Combined sources10
Helixi770 – 806Combined sources37
Helixi808 – 824Combined sources17
Beta strandi827 – 829Combined sources3
Helixi837 – 845Combined sources9
Helixi856 – 867Combined sources12
Helixi869 – 873Combined sources5
Helixi874 – 877Combined sources4
Turni878 – 880Combined sources3
Helixi881 – 885Combined sources5
Helixi889 – 914Combined sources26
Helixi924 – 959Combined sources36
Helixi969 – 994Combined sources26
Helixi996 – 999Combined sources4
Helixi1001 – 1022Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J1DX-ray2.55G596-1078[»]
2Z6EX-ray2.80A/B/C/D594-1012[»]
ProteinModelPortaliQ9Y4D1.
SMRiQ9Y4D1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y4D1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 420GBD/FH3PROSITE-ProRule annotationAdd BLAST376
Domaini528 – 599FH1Add BLAST72
Domaini600 – 1009FH2PROSITE-ProRule annotationAdd BLAST410
Domaini1027 – 1058DADPROSITE-ProRule annotationAdd BLAST32

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni693 – 702Actin-binding10

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili437 – 526Sequence analysisAdd BLAST90

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi528 – 593Pro-richAdd BLAST66

Domaini

The C-terminal DAD domain may participate in intramolecular interactions with the N-terminus.
The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).By similarity

Sequence similaritiesi

Belongs to the formin homology family.Curated
Contains 1 DAD (diaphanous autoregulatory) domain.PROSITE-ProRule annotation
Contains 1 FH2 (formin homology 2) domain.PROSITE-ProRule annotation
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1922. Eukaryota.
ENOG410XT5Z. LUCA.
GeneTreeiENSGT00760000118986.
HOGENOMiHOG000237318.
HOVERGENiHBG101333.
InParanoidiQ9Y4D1.
KOiK04512.
OMAiWNQYSGR.
OrthoDBiEOG091G02QJ.
PhylomeDBiQ9Y4D1.
TreeFamiTF314602.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027650. DAAM1.
IPR014767. DAD_dom.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR014768. GBD/FH3_dom.
IPR010473. GTPase-bd.
[Graphical view]
PANTHERiPTHR23213:SF181. PTHR23213:SF181. 2 hits.
PfamiPF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 1 hit.
PF02181. FH2. 1 hit.
[Graphical view]
SMARTiSM01139. Drf_FH3. 1 hit.
SM01140. Drf_GBD. 1 hit.
SM00498. FH2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS51231. DAD. 1 hit.
PS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y4D1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPRKRGGRG ISFIFCCFRN NDHPEITYRL RNDSNFALQT MEPALPMPPV
60 70 80 90 100
EELDVMFSEL VDELDLTDKH REAMFALPAE KKWQIYCSKK KDQEENKGAT
110 120 130 140 150
SWPEFYIDQL NSMAARKSLL ALEKEEEEER SKTIESLKTA LRTKPMRFVT
160 170 180 190 200
RFIDLDGLSC ILNFLKTMDY ETSESRIHTS LIGCIKALMN NSQGRAHVLA
210 220 230 240 250
HSESINVIAQ SLSTENIKTK VAVLEILGAV CLVPGGHKKV LQAMLHYQKY
260 270 280 290 300
ASERTRFQTL INDLDKSTGR YRDEVSLKTA IMSFINAVLS QGAGVESLDF
310 320 330 340 350
RLHLRYEFLM LGIQPVIDKL REHENSTLDR HLDFFEMLRN EDELEFAKRF
360 370 380 390 400
ELVHIDTKSA TQMFELTRKR LTHSEAYPHF MSILHHCLQM PYKRSGNTVQ
410 420 430 440 450
YWLLLDRIIQ QIVIQNDKGQ DPDSTPLENF NIKNVVRMLV NENEVKQWKE
460 470 480 490 500
QAEKMRKEHN ELQQKLEKKE RECDAKTQEK EEMMQTLNKM KEKLEKETTE
510 520 530 540 550
HKQVKQQVAD LTAQLHELSR RAVCASIPGG PSPGAPGGPF PSSVPGSLLP
560 570 580 590 600
PPPPPPLPGG MLPPPPPPLP PGGPPPPPGP PPLGAIMPPP GAPMGLALKK
610 620 630 640 650
KSIPQPTNAL KSFNWSKLPE NKLEGTVWTE IDDTKVFKIL DLEDLERTFS
660 670 680 690 700
AYQRQQDFFV NSNSKQKEAD AIDDTLSSKL KVKELSVIDG RRAQNCNILL
710 720 730 740 750
SRLKLSNDEI KRAILTMDEQ EDLPKDMLEQ LLKFVPEKSD IDLLEEHKHE
760 770 780 790 800
LDRMAKADRF LFEMSRINHY QQRLQSLYFK KKFAERVAEV KPKVEAIRSG
810 820 830 840 850
SEEVFRSGAL KQLLEVVLAF GNYMNKGQRG NAYGFKISSL NKIADTKSSI
860 870 880 890 900
DKNITLLHYL ITIVENKYPS VLNLNEELRD IPQAAKVNMT ELDKEISTLR
910 920 930 940 950
SGLKAVETEL EYQKSQPPQP GDKFVSVVSQ FITVASFSFS DVEDLLAEAK
960 970 980 990 1000
DLFTKAVKHF GEEAGKIQPD EFFGIFDQFL QAVSEAKQEN ENMRKKKEEE
1010 1020 1030 1040 1050
ERRARMEAQL KEQRERERKM RKAKENSEES GEFDDLVSAL RSGEVFDKDL
1060 1070
SKLKRNRKRI TNQMTDSSRE RPITKLNF
Length:1,078
Mass (Da):123,473
Last modified:August 15, 2003 - v2
Checksum:iE82D9892390254B6
GO
Isoform 2 (identifier: Q9Y4D1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     656-665: Missing.

Show »
Length:1,068
Mass (Da):122,306
Checksum:iC58B1A7445080313
GO
Isoform 3 (identifier: Q9Y4D1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     788-818: Missing.
     888-916: NMTELDKEISTLRSGLKAVETELEYQKSQ → KSWNIRSLSPHSPEISLCLLSASSSQ
     917-1078: Missing.

Show »
Length:882
Mass (Da):100,745
Checksum:i1BB2D1876B212852
GO

Sequence cautioni

The sequence BAA31641 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC04230 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti940S → F in AAH24781 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008000656 – 665Missing in isoform 2. 1 Publication10
Alternative sequenceiVSP_008001788 – 818Missing in isoform 3. 1 PublicationAdd BLAST31
Alternative sequenceiVSP_008002888 – 916NMTEL…YQKSQ → KSWNIRSLSPHSPEISLCLL SASSSQ in isoform 3. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_008003917 – 1078Missing in isoform 3. 1 PublicationAdd BLAST162

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014566 mRNA. Translation: BAA31641.1. Different initiation.
AL133502 Genomic DNA. No translation available.
BC024781 mRNA. Translation: AAH24781.1.
BC038428 mRNA. Translation: AAH38428.1.
BC064999 mRNA. Translation: AAH64999.1.
BX247986 mRNA. Translation: CAD62320.1.
AK093813 mRNA. Translation: BAC04230.1. Different initiation.
CCDSiCCDS58323.1. [Q9Y4D1-2]
CCDS9737.1. [Q9Y4D1-1]
RefSeqiNP_001257449.1. NM_001270520.1. [Q9Y4D1-2]
NP_055807.1. NM_014992.2. [Q9Y4D1-1]
XP_005267487.1. XM_005267430.1. [Q9Y4D1-1]
XP_005267488.1. XM_005267431.1. [Q9Y4D1-1]
UniGeneiHs.19156.
Hs.654934.

Genome annotation databases

EnsembliENST00000360909; ENSP00000354162; ENSG00000100592. [Q9Y4D1-2]
ENST00000395125; ENSP00000378557; ENSG00000100592. [Q9Y4D1-1]
GeneIDi23002.
KEGGihsa:23002.
UCSCiuc001xea.3. human. [Q9Y4D1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014566 mRNA. Translation: BAA31641.1. Different initiation.
AL133502 Genomic DNA. No translation available.
BC024781 mRNA. Translation: AAH24781.1.
BC038428 mRNA. Translation: AAH38428.1.
BC064999 mRNA. Translation: AAH64999.1.
BX247986 mRNA. Translation: CAD62320.1.
AK093813 mRNA. Translation: BAC04230.1. Different initiation.
CCDSiCCDS58323.1. [Q9Y4D1-2]
CCDS9737.1. [Q9Y4D1-1]
RefSeqiNP_001257449.1. NM_001270520.1. [Q9Y4D1-2]
NP_055807.1. NM_014992.2. [Q9Y4D1-1]
XP_005267487.1. XM_005267430.1. [Q9Y4D1-1]
XP_005267488.1. XM_005267431.1. [Q9Y4D1-1]
UniGeneiHs.19156.
Hs.654934.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J1DX-ray2.55G596-1078[»]
2Z6EX-ray2.80A/B/C/D594-1012[»]
ProteinModelPortaliQ9Y4D1.
SMRiQ9Y4D1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116648. 19 interactors.
DIPiDIP-29641N.
IntActiQ9Y4D1. 23 interactors.
MINTiMINT-1180756.
STRINGi9606.ENSP00000247170.

PTM databases

iPTMnetiQ9Y4D1.
PhosphoSitePlusiQ9Y4D1.
SwissPalmiQ9Y4D1.

Polymorphism and mutation databases

BioMutaiDAAM1.
DMDMi34098767.

Proteomic databases

EPDiQ9Y4D1.
PaxDbiQ9Y4D1.
PeptideAtlasiQ9Y4D1.
PRIDEiQ9Y4D1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000360909; ENSP00000354162; ENSG00000100592. [Q9Y4D1-2]
ENST00000395125; ENSP00000378557; ENSG00000100592. [Q9Y4D1-1]
GeneIDi23002.
KEGGihsa:23002.
UCSCiuc001xea.3. human. [Q9Y4D1-1]

Organism-specific databases

CTDi23002.
DisGeNETi23002.
GeneCardsiDAAM1.
H-InvDBHIX0011701.
HGNCiHGNC:18142. DAAM1.
HPAiHPA026605.
MIMi606626. gene.
neXtProtiNX_Q9Y4D1.
OpenTargetsiENSG00000100592.
PharmGKBiPA27129.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1922. Eukaryota.
ENOG410XT5Z. LUCA.
GeneTreeiENSGT00760000118986.
HOGENOMiHOG000237318.
HOVERGENiHBG101333.
InParanoidiQ9Y4D1.
KOiK04512.
OMAiWNQYSGR.
OrthoDBiEOG091G02QJ.
PhylomeDBiQ9Y4D1.
TreeFamiTF314602.

Enzyme and pathway databases

ReactomeiR-HSA-4086400. PCP/CE pathway.
R-HSA-5663220. RHO GTPases Activate Formins.
SignaLinkiQ9Y4D1.
SIGNORiQ9Y4D1.

Miscellaneous databases

ChiTaRSiDAAM1. human.
EvolutionaryTraceiQ9Y4D1.
GeneWikiiDAAM1.
GenomeRNAii23002.
PROiQ9Y4D1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100592.
CleanExiHS_DAAM1.
ExpressionAtlasiQ9Y4D1. baseline and differential.
GenevisibleiQ9Y4D1. HS.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027650. DAAM1.
IPR014767. DAD_dom.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR014768. GBD/FH3_dom.
IPR010473. GTPase-bd.
[Graphical view]
PANTHERiPTHR23213:SF181. PTHR23213:SF181. 2 hits.
PfamiPF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 1 hit.
PF02181. FH2. 1 hit.
[Graphical view]
SMARTiSM01139. Drf_FH3. 1 hit.
SM01140. Drf_GBD. 1 hit.
SM00498. FH2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS51231. DAD. 1 hit.
PS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDAAM1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y4D1
Secondary accession number(s): Q86U34, Q8N1Z8, Q8TB39
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: August 15, 2003
Last modified: November 2, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.