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Protein

Helicase ARIP4

Gene

RAD54L2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA helicase that modulates androgen receptor (AR)-dependent transactivation in a promoter-dependent manner. Not able to remodel mononucleosomes in vitro (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Enzyme regulationi

Enzyme activity is enhanced by dsDNA (double-stranded DNA) and ssDNA (single-stranded DNA).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi305 – 3128ATPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKQ9Y4B4.

Names & Taxonomyi

Protein namesi
Recommended name:
Helicase ARIP4 (EC:3.6.4.12)
Alternative name(s):
Androgen receptor-interacting protein 4
RAD54-like protein 2
Gene namesi
Name:RAD54L2
Synonyms:ARIP4, KIAA0809
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:29123. RAD54L2.

Subcellular locationi

  • Nucleus By similarity

  • Note: Localizes in speckle-like nuclear compartments.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA143485591.

Polymorphism and mutation databases

BioMutaiRAD54L2.
DMDMi296439458.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14671467Helicase ARIP4PRO_0000315781Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki1018 – 1018Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei1169 – 11691PhosphoserineCombined sources
Modified residuei1172 – 11721PhosphoserineCombined sources
Modified residuei1260 – 12601PhosphothreonineCombined sources

Post-translational modificationi

Sumoylated.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9Y4B4.
MaxQBiQ9Y4B4.
PaxDbiQ9Y4B4.
PeptideAtlasiQ9Y4B4.
PRIDEiQ9Y4B4.

PTM databases

iPTMnetiQ9Y4B4.
PhosphoSiteiQ9Y4B4.

Expressioni

Gene expression databases

BgeeiENSG00000164080.
CleanExiHS_RAD54L2.
ExpressionAtlasiQ9Y4B4. baseline and differential.
GenevisibleiQ9Y4B4. HS.

Organism-specific databases

HPAiHPA035004.
HPA039992.

Interactioni

Subunit structurei

Interacts with AR via its N-terminus. Interacts with DYRK1A. Binds DNA and mononucleosomes, but does not seem to form large multiprotein complexes (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PSMA3P257883EBI-948156,EBI-348380
SIAH1Q8IUQ43EBI-948156,EBI-747107
SUMO1P631653EBI-948156,EBI-80140

Protein-protein interaction databases

BioGridi116750. 24 interactions.
DIPiDIP-47284N.
IntActiQ9Y4B4. 10 interactions.
MINTiMINT-3086380.
STRINGi9606.ENSP00000386520.

Structurei

3D structure databases

ProteinModelPortaliQ9Y4B4.
SMRiQ9Y4B4. Positions 260-934.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini292 – 512221Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini728 – 896169Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi463 – 4664DEAH box
Motifi551 – 5555LXXLL motif 1
Motifi1329 – 13335LXXLL motif 2

Domaini

Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to be important for the association with nuclear receptors.By similarity

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1015. Eukaryota.
COG0553. LUCA.
GeneTreeiENSGT00550000074619.
HOGENOMiHOG000168429.
HOVERGENiHBG104283.
InParanoidiQ9Y4B4.
KOiK10876.
OMAiDGQQPPR.
OrthoDBiEOG091G01G9.
PhylomeDBiQ9Y4B4.
TreeFamiTF313172.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Y4B4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDESASGSD PDLDPDVELE DAEEEEEEEE VAVEECDRDD EEDLLDDPSL
60 70 80 90 100
EGMCGTEHAQ LGEDGQQPPR CTSTTSSQSE PSEQLRRHQG KNLASEDPKK
110 120 130 140 150
KRAQKPSHMR RNIRKLLRED QLEPVTKAAQ QEELERRKRL EQQRKDYAAP
160 170 180 190 200
IPTVPLEFLP EEIALRASDG PQLPPRVLAQ EVICLDSSSG SEDEKSSRDE
210 220 230 240 250
VIELSSGEED TLHIVDSSES VSEDDEEEEK GGTHVNDVLN QRDALGRVLV
260 270 280 290 300
NLNHPPEEEN VFLAPQLARA VKPHQIGGIR FLYDNLVESL ERFKTSSGFG
310 320 330 340 350
CILAHSMGLG KTLQVISFID VLFRHTPAKT VLAIVPVNTL QNWLAEFNMW
360 370 380 390 400
LPPPEALPAD NKPEEVQPRF FKVHILNDEH KTMASRAKVM ADWVSEGGVL
410 420 430 440 450
LMGYEMYRLL TLKKSFATGR PKKTKKRSHP VIIDLDEEDR QQEFRREFEK
460 470 480 490 500
ALCRPGPDVV ICDEGHRIKN CQASTSQALK NIRSRRRVVL TGYPLQNNLI
510 520 530 540 550
EYWCMVDFVR PDFLGTRQEF SNMFERPILN GQCIDSTPQD VRLMRYRSHV
560 570 580 590 600
LHSLLEGFVQ RRGHTVLKIH LPAKEENVIL VRLSKIQRDL YTQFMDRFRD
610 620 630 640 650
CGSSGWLGLN PLKAFCVCCK IWNHPDVLYE ALQKESLANE QDLDVEELGS
660 670 680 690 700
AGTSARCPPQ GTKGKGEDST LASSMGEATN SKFLQGVGFN PFQERGNNIV
710 720 730 740 750
TYEWAKDLLT NYQTGVLENS PKMVLLFHLI EESVKLGDKI LVFSQSLSTL
760 770 780 790 800
ALIEEFLGKR EVPCPPGTEG QGAQKWVRNI SYFRLDGSTP AFERERLINQ
810 820 830 840 850
FNDPSNLTTW LFLLSTRAGC LGVNLIGANR VVVFDASWNP CHDAQAVCRV
860 870 880 890 900
YRYGQKKPCY IYRLVADYTL EKKIYDRQIS KQGMSDRVVD DLNPMLNFTR
910 920 930 940 950
KEVENLLHFV EKEPAPQVSL NVKGIKESVL QLACLKYPHL ITKEPFEHES
960 970 980 990 1000
LLLNRKDHKL TKAEKKAAKK SYEEDKRTSV PYTRPSYAQY YPASDQSLTS
1010 1020 1030 1040 1050
IPAFSQRNWQ PTLKGDEKPV ASVRPVQSTP IPMMPRHVPL GGSVSSASST
1060 1070 1080 1090 1100
NPSMNFPINY LQRAGVLVQK VVTTTDIVIP GLNSSTDVQA RINAGESIHI
1110 1120 1130 1140 1150
IRGTKGTYIR TSDGRIFAVR ATGKPKVPED GRMAASGSQG PSCESTSNGR
1160 1170 1180 1190 1200
HSASSPKAPD PEGLARPVSP DSPEIISELQ QYADVAAARE SRQSSPSTNA
1210 1220 1230 1240 1250
ALPGPPAQLM DSSAVPGTAL GTEPRLGGHC LNSSLLVTGQ PCGDRHPVLD
1260 1270 1280 1290 1300
LRGHKRKLAT PPAAQESSRR RSRKGHLPAP VQPYEHGYPV SGGFAMPPVS
1310 1320 1330 1340 1350
LNHNLTTPFT SQAGENSLFM GSTPSYYQLS NLLADARLVF PVTTDPLVPA
1360 1370 1380 1390 1400
GPVSSSSTAT SVTASNPSFM LNPSVPGILP SYSLPFSQPL LSEPRMFAPF
1410 1420 1430 1440 1450
PSPVLPSNLS RGMSIYPGYM SPHAGYPAGG LLRSQVPPFD SHEVAEVGFS
1460
SNDDEDKDDD VIEVTGK
Length:1,467
Mass (Da):162,769
Last modified:May 18, 2010 - v4
Checksum:i9B4144B6BBC4C3B1
GO

Sequence cautioni

The sequence AAH24298 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti123 – 1231E → Q in BAA34529 (PubMed:9872452).Curated
Sequence conflicti249 – 2491L → F in AAH24298 (PubMed:15489334).Curated
Sequence conflicti328 – 3281A → V in BAA34529 (PubMed:9872452).Curated
Sequence conflicti860 – 8601Y → F in AAH01474 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti1369 – 13691F → L.
Corresponds to variant rs35712917 [ dbSNP | Ensembl ].
VAR_038302

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC099050 Genomic DNA. No translation available.
AC113933 Genomic DNA. No translation available.
AB018352 mRNA. Translation: BAA34529.2.
BC001474 mRNA. Translation: AAH01474.2.
BC024298 mRNA. Translation: AAH24298.1. Different initiation.
CCDSiCCDS33765.2.
RefSeqiNP_001309182.1. NM_001322253.1.
NP_001309185.1. NM_001322256.1.
NP_055921.2. NM_015106.3.
XP_006713125.1. XM_006713062.3.
UniGeneiHs.105399.
Hs.655185.
Hs.659486.

Genome annotation databases

EnsembliENST00000409535; ENSP00000386520; ENSG00000164080.
GeneIDi23132.
KEGGihsa:23132.
UCSCiuc011bdt.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC099050 Genomic DNA. No translation available.
AC113933 Genomic DNA. No translation available.
AB018352 mRNA. Translation: BAA34529.2.
BC001474 mRNA. Translation: AAH01474.2.
BC024298 mRNA. Translation: AAH24298.1. Different initiation.
CCDSiCCDS33765.2.
RefSeqiNP_001309182.1. NM_001322253.1.
NP_001309185.1. NM_001322256.1.
NP_055921.2. NM_015106.3.
XP_006713125.1. XM_006713062.3.
UniGeneiHs.105399.
Hs.655185.
Hs.659486.

3D structure databases

ProteinModelPortaliQ9Y4B4.
SMRiQ9Y4B4. Positions 260-934.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116750. 24 interactions.
DIPiDIP-47284N.
IntActiQ9Y4B4. 10 interactions.
MINTiMINT-3086380.
STRINGi9606.ENSP00000386520.

PTM databases

iPTMnetiQ9Y4B4.
PhosphoSiteiQ9Y4B4.

Polymorphism and mutation databases

BioMutaiRAD54L2.
DMDMi296439458.

Proteomic databases

EPDiQ9Y4B4.
MaxQBiQ9Y4B4.
PaxDbiQ9Y4B4.
PeptideAtlasiQ9Y4B4.
PRIDEiQ9Y4B4.

Protocols and materials databases

DNASUi23132.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000409535; ENSP00000386520; ENSG00000164080.
GeneIDi23132.
KEGGihsa:23132.
UCSCiuc011bdt.3. human.

Organism-specific databases

CTDi23132.
GeneCardsiRAD54L2.
HGNCiHGNC:29123. RAD54L2.
HPAiHPA035004.
HPA039992.
neXtProtiNX_Q9Y4B4.
PharmGKBiPA143485591.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1015. Eukaryota.
COG0553. LUCA.
GeneTreeiENSGT00550000074619.
HOGENOMiHOG000168429.
HOVERGENiHBG104283.
InParanoidiQ9Y4B4.
KOiK10876.
OMAiDGQQPPR.
OrthoDBiEOG091G01G9.
PhylomeDBiQ9Y4B4.
TreeFamiTF313172.

Enzyme and pathway databases

SABIO-RKQ9Y4B4.

Miscellaneous databases

ChiTaRSiRAD54L2. human.
GenomeRNAii23132.
PROiQ9Y4B4.

Gene expression databases

BgeeiENSG00000164080.
CleanExiHS_RAD54L2.
ExpressionAtlasiQ9Y4B4. baseline and differential.
GenevisibleiQ9Y4B4. HS.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARIP4_HUMAN
AccessioniPrimary (citable) accession number: Q9Y4B4
Secondary accession number(s): Q8TB57, Q9BV54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 18, 2010
Last modified: September 7, 2016
This is version 126 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.